FastQCFastQC Report
Fri 27 May 2016
SRR940011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519058
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69391.3368448227365728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48020.9251374605535412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.665821545954402No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.32693841536013313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.24525197569443108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.234463200644244No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.2026748455856571No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.20228953219100756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.072.759911
TATCACG753.1832315E-1063.572172
TATAACG950.062.735692
GTATCAA37800.059.920031
ATAACGC1103.6379788E-1254.1808243
ATCACGC1004.0781742E-947.6791273
TATCAAC48500.046.6960532
TCAACGC49250.046.347994
ATCAACG49100.046.246813
CAACGCA49600.046.0788425
AACGCAG51500.044.4815526
TAACGCA1352.7284841E-1144.147344
ACGCAGA59150.038.7249267
CGCAGAG59400.038.561948
GTGGTAT7300.037.55911
TGGTATC7350.035.6782572
GCAGAGT64200.035.2154439
GTATAAC2750.034.6790771
TCACGCA1551.9388426E-730.7607274
ATACGTC800.005351386529.759255