FastQCFastQC Report
Fri 27 May 2016
SRR940010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600952
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.049.2757871
GTATCAA32200.048.3855861
TCAACGC36650.040.5205734
ATCAACG36650.040.512963
CAACGCA37700.039.5143135
TATCAAC38400.039.0163462
AACGCAG40000.037.0932736
GTACATG85300.034.710751
TACATGG87850.032.410132
ACGCAGA47200.031.4349777
ACATGGG89050.031.1377753
CGCAGAG48850.030.129248
TACGGTT1004.377974E-429.8208644
CATGGGG53550.027.5098174
CATGGGA40100.025.8794524
GAGTACA64900.025.3860871
TAAGGTG7300.025.3043175
AGTACAT64200.024.4525892
GCAGAGT58850.024.1897019
ATGGGGA26200.024.1079545
Produced by FastQC (version 0.11.5)