FastQCFastQC Report
Fri 27 May 2016
SRR940010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600952
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70400.4397383556783714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48370.3021327310250401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.20650213123191702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.071.542341
GTATCAA64500.062.517241
ATCAACG81500.049.0300143
TATCAAC82250.048.582932
TCAACGC82250.048.5814134
CAACGCA83250.047.8348545
AACGCAG86950.045.7821666
GTGGTAT11650.040.9615941
ACGCAGA98050.040.5309947
TATAACG1053.20475E-739.7606542
CGCAGAG100750.039.385758
TATATCG851.7064899E-435.0149655
GTACATG80700.034.888421
GCAGAGT113200.034.7912459
TACATGG82000.033.8935362
ACATGGG84600.031.8649233
TGGTATC15350.030.694712
TAAGGTG6900.030.1940615
GGACCGA5350.028.9174066
CATGGGG53550.028.7336944
Produced by FastQC (version 0.11.5)