FastQCFastQC Report
Fri 27 May 2016
SRR940009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274011
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26550.055.071591
GGTATCA15200.046.7228621
ATCAACG30400.045.883373
TCAACGC30750.045.1761554
CAACGCA31850.043.5884675
AACGCAG33800.041.0737466
TATCAAC36300.039.7723122
GTACATG71700.036.956421
TACATGG73000.035.141292
ACGCAGA39600.035.0578967
CGCAGAG40700.033.963998
ACATGGG77200.032.893143
CATGGGA48450.029.4105134
GCAGAGT49000.027.109059
CATGGGG30450.026.628714
CCCGTAC1159.941808E-425.9170283
ATGGGGA16350.022.2298835
GAGTACA55850.021.7988241
AGTACAT53750.021.754532
GTACTAG3859.094947E-1221.7016811
Produced by FastQC (version 0.11.5)