##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940009_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1274011 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.651765958064725 34.0 31.0 34.0 30.0 34.0 2 31.722602866066307 34.0 31.0 34.0 30.0 34.0 3 31.87210863956434 34.0 31.0 34.0 30.0 34.0 4 35.03230034905507 37.0 35.0 37.0 33.0 37.0 5 35.16200723541633 37.0 37.0 37.0 33.0 37.0 6 35.232292342844765 37.0 37.0 37.0 33.0 37.0 7 35.261952997266114 37.0 37.0 37.0 35.0 37.0 8 35.25220425883293 37.0 37.0 37.0 35.0 37.0 9 36.889794515118 39.0 39.0 39.0 35.0 39.0 10-11 36.918767969821296 39.0 39.0 39.0 35.0 39.0 12-13 36.871532113929945 39.0 39.0 39.0 35.0 39.0 14-15 38.41050430490789 41.0 40.0 41.0 34.5 41.0 16-17 38.469994764566394 41.0 40.0 41.0 35.0 41.0 18-19 38.51173145286815 41.0 40.0 41.0 35.0 41.0 20-21 38.52860100893949 41.0 40.0 41.0 35.0 41.0 22-23 38.5845769777498 41.0 40.0 41.0 34.5 41.0 24-25 38.67157073212084 41.0 40.0 41.0 34.5 41.0 26-27 38.72231872409265 41.0 40.0 41.0 34.0 41.0 28-29 38.68821462295067 41.0 40.0 41.0 34.0 41.0 30-31 38.63956237426521 41.0 40.0 41.0 34.0 41.0 32-33 38.59401567176421 41.0 40.0 41.0 34.0 41.0 34-35 38.52072470331889 41.0 39.5 41.0 34.0 41.0 36-37 38.40436699526142 41.0 39.0 41.0 33.0 41.0 38-39 38.319102425332275 41.0 39.0 41.0 33.0 41.0 40-41 38.22750980956994 41.0 39.0 41.0 33.0 41.0 42-43 38.08585169201836 41.0 39.0 41.0 33.0 41.0 44-45 37.95982373778563 40.5 38.0 41.0 32.5 41.0 46-47 37.81388308264214 40.0 38.0 41.0 32.0 41.0 48-49 37.67861384242365 40.0 38.0 41.0 32.0 41.0 50-51 37.524967209859255 40.0 38.0 41.0 31.0 41.0 52-53 37.656567329481454 40.0 38.0 41.0 32.0 41.0 54-55 37.46593004299021 40.0 37.0 41.0 31.0 41.0 56-57 37.357582469853085 40.0 37.0 41.0 31.0 41.0 58-59 37.16803858051461 40.0 36.5 41.0 31.0 41.0 60-61 36.932065735696156 40.0 36.0 41.0 31.0 41.0 62-63 36.649211427530844 39.0 35.0 41.0 31.0 41.0 64-65 36.32631978844766 39.0 35.0 41.0 31.0 41.0 66-67 35.99185446593475 38.5 35.0 41.0 30.5 41.0 68-69 35.62241495560085 37.0 35.0 40.0 30.0 41.0 70-71 35.24216509904546 37.0 35.0 39.5 30.0 41.0 72-73 34.85336115622236 36.5 35.0 39.0 30.0 41.0 74-75 34.46009885314962 36.0 35.0 39.0 29.5 41.0 76-77 34.079989105274606 35.5 35.0 37.5 29.0 39.5 78-79 33.73407097740915 35.0 34.5 37.0 29.0 39.0 80-81 33.40794467237724 35.0 34.0 37.0 29.0 39.0 82-83 33.09361575371013 35.0 34.0 36.0 28.5 38.0 84-85 32.84442010312313 35.0 34.0 36.0 28.0 37.0 86-87 32.63477277668717 35.0 34.0 35.5 28.0 37.0 88-89 32.45986494622103 35.0 34.0 35.0 28.0 36.0 90-91 32.30465788756926 35.0 34.0 35.0 27.0 36.0 92-93 32.158098713433404 35.0 34.0 35.0 27.0 36.0 94-95 32.03877635279444 35.0 34.0 35.0 27.0 36.0 96-97 31.934925601113335 35.0 34.0 35.0 27.0 35.5 98-99 31.86120135540431 35.0 34.0 35.0 26.5 35.0 100-101 31.76395808199458 35.0 33.5 35.0 25.5 35.0 102-103 31.087124443980468 34.0 32.0 35.0 25.0 35.0 104-105 31.960019575969127 35.0 34.0 35.0 27.0 35.0 106-107 32.05250464870397 35.0 34.0 35.0 27.0 35.0 108-109 32.03238158854201 35.0 34.0 35.0 27.0 35.0 110-111 31.976293768264167 35.0 34.0 35.0 27.0 35.0 112-113 31.91812040869349 35.0 34.0 35.0 27.0 35.0 114-115 31.85209468364088 35.0 34.0 35.0 26.0 35.0 116-117 31.772594977594384 35.0 34.0 35.0 25.0 35.0 118-119 31.678474126204563 35.0 34.0 35.0 25.0 35.0 120-121 31.578619807835253 35.0 34.0 35.0 25.0 35.0 122-123 31.439865903826576 35.0 34.0 35.0 25.0 35.0 124-125 30.222642112195267 34.5 31.5 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10007381290336781 1101 2 0.16746378125481343 1101 3 0.2991553060928105 1101 4 0.20010908172662312 1101 5 0.2442264049644507 1101 6 0.3454272810282788 1101 7 0.3205847379178479 1101 8 0.3538188996806042 1101 9 0.36490679053112984 1101 10-11 0.3074688463673567 1101 12-13 0.3574655511710816 1101 14-15 0.565045657030737 1101 16-17 0.5343678543456889 1101 18-19 0.539536191700293 1101 20-21 0.4163049905534635 1101 22-23 0.46259253659035693 1101 24-25 0.3884983797262507 1101 26-27 0.42318069406905323 1101 28-29 0.43678218943906444 1101 30-31 0.4600940102340445 1101 32-33 0.4722424170946766 1101 34-35 0.4374109616124713 1101 36-37 0.5927807926025537 1101 38-39 0.5836245557774191 1101 40-41 0.5801110331308621 1101 42-43 0.6379804516047898 1101 44-45 0.6664652490022576 1101 46-47 0.6366747301930928 1101 48-49 0.6225945913874682 1101 50-51 0.6892765554082487 1101 52-53 0.7895930268900173 1101 54-55 0.8855678212058322 1101 56-57 0.802563983779585 1101 58-59 0.712794067253725 1101 60-61 0.6708456572765655 1101 62-63 0.6723977919564632 1101 64-65 0.6307494300787511 1101 66-67 0.5260447365941445 1101 68-69 0.4642947210121875 1101 70-71 0.4750895779071982 1101 72-73 0.5420443526023178 1101 74-75 0.4003256744352015 1101 76-77 0.37965903305570237 1101 78-79 0.25712468373190944 1101 80-81 0.2885798738094749 1101 82-83 0.3048869495077682 1101 84-85 0.3173728046376354 1101 86-87 0.32191540320712875 1101 88-89 0.3276051393650832 1101 90-91 0.4385143992931617 1101 92-93 0.49134614869092985 1101 94-95 0.4526750960191137 1101 96-97 0.4706377121822669 1101 98-99 0.376712162902443 1101 100-101 0.4659409849978502 1101 102-103 0.44917599364081084 1101 104-105 0.5091728002242384 1101 106-107 0.5539484590763877 1101 108-109 0.5183851232075014 1101 110-111 0.5302487907866151 1101 112-113 0.574914472769148 1101 114-115 0.50168899658253 1101 116-117 0.528836994920713 1101 118-119 0.6477660183594267 1101 120-121 0.7141712071243091 1101 122-123 0.6521846925997608 1101 124-125 0.5502962637100524 1102 1 0.08475192018421396 1102 2 0.1497709717811837 1102 3 0.17071434066554403 1102 4 0.15924902853780054 1102 5 0.11253138765300008 1102 6 0.36907372590760446 1102 7 0.30420534105899577 1102 8 0.3107058483483769 1102 9 0.45670266588864905 1102 10-11 0.38900517533519974 1102 12-13 0.4046392678653419 1102 14-15 0.5378767072590449 1102 16-17 0.47180575890688203 1102 18-19 0.47377084973631867 1102 20-21 0.37169537642330397 1102 22-23 0.2885616017717467 1102 24-25 0.31257133873071297 1102 26-27 0.3500981259522362 1102 28-29 0.4120926666331002 1102 30-31 0.44934020924051765 1102 32-33 0.501836225113145 1102 34-35 0.5390486504076932 1102 36-37 0.5311407203460448 1102 38-39 0.5581466090665899 1102 40-41 0.5532500516465859 1102 42-43 0.4834318039054608 1102 44-45 0.5313681543161266 1102 46-47 0.5615756283816609 1102 48-49 0.5092007934046308 1102 50-51 0.48847370529033185 1102 52-53 0.48100378508159736 1102 54-55 0.5647714942451216 1102 56-57 0.5338444042723367 1102 58-59 0.40836961043201114 1102 60-61 0.44949235304655133 1102 62-63 0.5158464095489137 1102 64-65 0.5342989057172645 1102 66-67 0.4908422678300326 1102 68-69 0.5268831599704811 1102 70-71 0.6096604294301073 1102 72-73 0.5246074516037709 1102 74-75 0.47357947542872836 1102 76-77 0.6309818540703063 1102 78-79 0.47699083356381067 1102 80-81 0.4312789555496508 1102 82-83 0.5304338911506008 1102 84-85 0.4792995194753047 1102 86-87 0.42164255961237274 1102 88-89 0.45665702355395865 1102 90-91 0.4734735041152831 1102 92-93 0.37293140133783 1102 94-95 0.31749771632119206 1102 96-97 0.347859348992948 1102 98-99 0.3929134273913846 1102 100-101 0.37849241758353 1102 102-103 0.44195285148616037 1102 104-105 0.45162287298252934 1102 106-107 0.45791566766688163 1102 108-109 0.4253140960611361 1102 110-111 0.3678886623305928 1102 112-113 0.4458707425267896 1102 114-115 0.3224690156502277 1102 116-117 0.333955164422445 1102 118-119 0.4153885784477467 1102 120-121 0.4641906511506022 1102 122-123 0.4569014370425357 1102 124-125 0.3591614852465028 1103 1 -0.13206782723254662 1103 2 0.015997531291326084 1103 3 0.002430758575993508 1103 4 -0.1269737039535883 1103 5 0.01038825715165359 1103 6 0.08735730799715213 1103 7 -0.020957307237978284 1103 8 -0.05050731659613206 1103 9 0.012366653642189362 1103 10-11 0.018836786510092907 1103 12-13 -0.014060502513522977 1103 14-15 0.12394635900033535 1103 16-17 0.08519554076681146 1103 18-19 0.10425783787255938 1103 20-21 -0.04705701546342311 1103 22-23 0.0899967338039005 1103 24-25 0.030969731382839427 1103 26-27 0.11532966058675953 1103 28-29 0.22773082201005934 1103 30-31 0.20505681850190172 1103 32-33 0.24277575821685815 1103 34-35 0.17841910582292542 1103 36-37 0.2557532346973659 1103 38-39 0.2591607690359794 1103 40-41 0.20900971486894093 1103 42-43 0.15239468182356575 1103 44-45 0.20165135370380227 1103 46-47 0.1682968300655503 1103 48-49 0.1862099782496358 1103 50-51 0.15422188745642273 1103 52-53 0.020350321629628354 1103 54-55 0.1488654475554938 1103 56-57 0.1287066315972467 1103 58-59 0.12337630771482822 1103 60-61 0.07650632166499349 1103 62-63 -0.03818152768797489 1103 64-65 -0.026282801133099554 1103 66-67 -0.0737960788978711 1103 68-69 -0.042515041330702275 1103 70-71 -0.021955529238084637 1103 72-73 0.038241205909166354 1103 74-75 -0.053903302986888946 1103 76-77 -0.11408129173910453 1103 78-79 -0.14898505621804503 1103 80-81 -0.07738445815107298 1103 82-83 0.00757702165193308 1103 84-85 -0.014534196559893076 1103 86-87 -0.0266639845896961 1103 88-89 -0.041381629335440095 1103 90-91 -0.011084475218815726 1103 92-93 -0.05358027106936447 1103 94-95 -0.12640297254218424 1103 96-97 -0.09413261426767505 1103 98-99 -0.18721477773682338 1103 100-101 -0.237935060411111 1103 102-103 -0.2815593949738435 1103 104-105 -0.21532603631712277 1103 106-107 -0.12932122479389108 1103 108-109 -0.15524464867671384 1103 110-111 -0.13690467865677647 1103 112-113 -0.1532299080663968 1103 114-115 -0.18892593268502011 1103 116-117 -0.2169059147968433 1103 118-119 -0.15884123402833694 1103 120-121 -0.17273051226309377 1103 122-123 -0.18602336203898062 1103 124-125 -0.06712056603554828 1104 1 -0.28540815827321 1104 2 -0.19399277013184957 1104 3 -0.20006501364249374 1104 4 -0.30865935343597783 1104 5 -0.3578960155011188 1104 6 -0.20377225394022958 1104 7 -0.13017870487656324 1104 8 -0.21063741870741381 1104 9 -0.13796665820695608 1104 10-11 -0.27020823360832225 1104 12-13 -0.3061735415748572 1104 14-15 -0.28720040563423765 1104 16-17 -0.16971340463572204 1104 18-19 -0.29772169997183795 1104 20-21 -0.3553839035400017 1104 22-23 -0.23534122016469183 1104 24-25 -0.26223765856440195 1104 26-27 -0.13030195155610613 1104 28-29 -0.04291433284490154 1104 30-31 -0.05944769471240363 1104 32-33 0.027081404568683354 1104 34-35 0.03229573593577584 1104 36-37 -0.0065508755349412695 1104 38-39 -0.035223263914623715 1104 40-41 -0.11977645146165372 1104 42-43 -0.07793424111265068 1104 44-45 -0.02614857324995512 1104 46-47 -0.04916402267261333 1104 48-49 -0.037484933987443014 1104 50-51 -0.15827331696564073 1104 52-53 -0.05581861008440825 1104 54-55 0.0454046899616003 1104 56-57 -0.19239394970352208 1104 58-59 -0.15613433616632477 1104 60-61 -0.19855095671791645 1104 62-63 -0.17834303508006855 1104 64-65 -0.21487005456366148 1104 66-67 -0.21361288014763602 1104 68-69 -0.19753631835090601 1104 70-71 -0.22349870785457426 1104 72-73 -0.22261252973095225 1104 74-75 -0.20104563727473845 1104 76-77 -0.2617123913305974 1104 78-79 -0.3036579824566772 1104 80-81 -0.3203439742657537 1104 82-83 -0.2840708463492021 1104 84-85 -0.3191615839904216 1104 86-87 -0.36778130821217303 1104 88-89 -0.3164590491457062 1104 90-91 -0.29841785765886897 1104 92-93 -0.3404101178257122 1104 94-95 -0.4418950295305315 1104 96-97 -0.4056506717286723 1104 98-99 -0.3242700933856 1104 100-101 -0.2523956461314967 1104 102-103 -0.40932682709147983 1104 104-105 -0.3539650436248145 1104 106-107 -0.38762582302420157 1104 108-109 -0.31382386088218794 1104 110-111 -0.2892567369955614 1104 112-113 -0.2856682330976277 1104 114-115 -0.3227179534571647 1104 116-117 -0.2646575944000631 1104 118-119 -0.31266706937964983 1104 120-121 -0.2534260859062911 1104 122-123 -0.20599579322051653 1104 124-125 -0.26864226046285467 1105 1 0.053691103744338164 1105 2 0.0689323158522761 1105 3 0.1277540518568827 1105 4 0.14569723736867957 1105 5 0.07822147374019295 1105 6 0.17984388234562942 1105 7 0.016551448621711984 1105 8 0.18013100802624393 1105 9 0.23465323517495307 1105 10-11 0.16984730252208635 1105 12-13 0.2779036388734042 1105 14-15 0.41729908993052334 1105 16-17 0.39058757877173633 1105 18-19 0.3517410894845412 1105 20-21 0.2726418727669113 1105 22-23 0.22275464999909644 1105 24-25 0.12147164420141365 1105 26-27 0.2179808899992608 1105 28-29 0.13763302579259573 1105 30-31 0.19195221516480387 1105 32-33 0.1421282736181908 1105 34-35 0.25519115711878726 1105 36-37 0.2962962327037246 1105 38-39 0.1794555047437214 1105 40-41 0.1760573736867599 1105 42-43 0.1653488639813645 1105 44-45 0.14575304740138506 1105 46-47 0.21732403160298475 1105 48-49 0.08138213192171406 1105 50-51 0.06450351182555636 1105 52-53 0.18385253080037955 1105 54-55 0.3353259698537485 1105 56-57 0.20511048776766216 1105 58-59 0.0869889754232176 1105 60-61 0.11650151716762736 1105 62-63 0.09234252173997959 1105 64-65 -0.02625024729579195 1105 66-67 0.0471784707366254 1105 68-69 0.038497989184591574 1105 70-71 0.14814163835355032 1105 72-73 0.09919321481768861 1105 74-75 0.08874424447436269 1105 76-77 0.06930569929848218 1105 78-79 0.0750897412746383 1105 80-81 0.1392119371288416 1105 82-83 0.10039362455798795 1105 84-85 0.11280294444985373 1105 86-87 0.15111701115360887 1105 88-89 0.1990318268493354 1105 90-91 0.1711361904061235 1105 92-93 0.07305752371124896 1105 94-95 0.11277680546309199 1105 96-97 0.11039753283076692 1105 98-99 0.119945270397956 1105 100-101 0.0627086075307659 1105 102-103 0.1311502966402891 1105 104-105 0.17641112624551525 1105 106-107 0.1419027545871785 1105 108-109 0.144390972854616 1105 110-111 0.2171643264979508 1105 112-113 0.1699971426008382 1105 114-115 0.13064498528829205 1105 116-117 0.2456324762802602 1105 118-119 0.21904034184679944 1105 120-121 0.08987680314542246 1105 122-123 -0.017263146869339607 1105 124-125 0.054419746840508765 1106 1 0.09120496257289545 1106 2 0.1843023125103791 1106 3 0.3274882778924031 1106 4 0.20318717223756266 1106 5 0.30376790242944196 1106 6 0.3709608931702135 1106 7 0.31074013522788135 1106 8 0.38939537495208754 1106 9 0.40274343481380015 1106 10-11 0.268633319340573 1106 12-13 0.3945731024058503 1106 14-15 0.45438445457727994 1106 16-17 0.5129016911827975 1106 18-19 0.4518961362495659 1106 20-21 0.36121968802747517 1106 22-23 0.4158151239746104 1106 24-25 0.4519955617067879 1106 26-27 0.4713249374799986 1106 28-29 0.5115345388252734 1106 30-31 0.4418622134579948 1106 32-33 0.39147208587534266 1106 34-35 0.40849525961890265 1106 36-37 0.48172520737271896 1106 38-39 0.5056191801982877 1106 40-41 0.41752872589248113 1106 42-43 0.3966679519449485 1106 44-45 0.4104183055951225 1106 46-47 0.41977372746175945 1106 48-49 0.3947842107818502 1106 50-51 0.34983796677378365 1106 52-53 0.24097062594832153 1106 54-55 0.3498743334004004 1106 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74-75 -0.6229359475842031 2216 76-77 -0.5154402415822332 2216 78-79 -0.5230683626476278 2216 80-81 -0.5368190861756759 2216 82-83 -0.5706359070489881 2216 84-85 -0.48864119898537695 2216 86-87 -0.500689344479099 2216 88-89 -0.5105482753069168 2216 90-91 -0.552632558430048 2216 92-93 -0.6222811717241612 2216 94-95 -0.6073177621548567 2216 96-97 -0.6325472383188 2216 98-99 -0.6186069019742071 2216 100-101 -0.6650061318426417 2216 102-103 -0.7049454803539312 2216 104-105 -0.6244815896651907 2216 106-107 -0.6636952159032319 2216 108-109 -0.6470658049585332 2216 110-111 -0.6184578114950838 2216 112-113 -0.6589636147410083 2216 114-115 -0.6398149981728878 2216 116-117 -0.6562747941935392 2216 118-119 -0.693380993632978 2216 120-121 -0.6467254502189093 2216 122-123 -0.6343481896480228 2216 124-125 -0.7337872327022126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15211.0 3 2592.0 4 1720.0 5 1345.0 6 1733.0 7 2076.0 8 2114.0 9 2334.0 10 2749.0 11 3403.0 12 3971.0 13 4132.0 14 4235.0 15 3974.0 16 3367.0 17 3129.0 18 2758.0 19 2516.0 20 2191.0 21 2154.0 22 2230.0 23 3116.0 24 5981.0 25 10970.0 26 12020.0 27 8504.0 28 8857.0 29 10763.0 30 12210.0 31 14405.0 32 19028.0 33 26021.0 34 39658.0 35 71238.0 36 180510.0 37 490634.0 38 288837.0 39 1325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.848267044472856 19.28767093164619 13.459502590087613 23.40455943379334 2 15.157238889041855 20.166698789683398 41.0987645094422 23.577297811832544 3 19.279807232548116 25.87191705158131 28.0698365889143 26.778439126956272 4 12.375510178422969 16.986092972306956 37.517150010136774 33.1212468391333 5 13.778012963846948 39.021948722458106 33.22189910308268 13.978139210612264 6 31.31814198347159 36.84103894635336 18.166248378413187 13.674570691761867 7 29.010314216500387 32.92838156711701 20.488030875903522 17.57327334047908 8 25.739165829153627 36.499591000277384 19.692884819234695 18.0683583513343 9 26.582767965493176 17.99040813375167 19.723451814312032 35.70337208644312 10-11 24.693378001432396 28.148402063151018 26.794577003782027 20.363642931634562 12-13 25.204508631624574 25.268304096689587 27.225627482379693 22.301559789306154 14-15 22.66450993999265 26.426077290468847 24.951367671394614 25.958045098143884 16-17 22.534024087947156 29.49128560918291 25.684348801766355 22.29034150110358 18-19 22.527367880787292 28.50217965236302 27.123428288239538 21.84702417861015 20-21 23.68224650389522 27.56657580700212 26.894133024211243 21.85704466489142 22-23 23.289053945048714 27.45489714265732 26.655565714402464 22.6004831978915 24-25 22.817158419551447 27.65007293616573 26.86399624203967 22.66877240224315 26-27 22.575557505978185 27.89543554001498 26.812022000348563 22.716984953658272 28-29 22.640761487305404 27.7666006242111 26.935588196507183 22.657049691976315 30-31 22.888054619564684 27.625308844279683 26.880510871122397 22.606125665033233 32-33 22.671879973577607 27.68306788615764 26.802164500975138 22.84288763928961 34-35 23.084419840754606 27.75447195700076 26.649778668841645 22.51132953340299 36-37 23.075446749738905 27.382439598367757 26.603917025098678 22.938196626794664 38-39 22.751084967145818 27.672795025302488 26.700023903228427 22.876096104323267 40-41 22.923500023745447 27.454641292662814 26.92757089585483 22.69428778773691 42-43 22.720660791118696 27.26732853586756 26.857827387003507 23.154183286010234 44-45 22.758812340691765 27.547695324551153 26.88742231331842 22.80607002143866 46-47 22.945401475440594 27.71934755285216 26.508275703432826 22.826975268274417 48-49 22.74100074424622 27.60191776864305 26.691315243230342 22.96576624388038 50-51 22.891852842714847 27.581334391533147 26.73563805798354 22.791174707768466 52-53 22.94703795989694 27.507540323656908 26.771917181261372 22.773504535184774 54-55 22.851090170507558 27.552638158852798 26.802175670005322 22.794096000634315 56-57 22.72514771844529 27.829319005504434 26.683252321906686 22.762280954143595 58-59 22.853714324743702 27.400119880872797 26.659755394344874 23.086410400038627 60-61 22.794719100947187 27.682682902704663 26.778040842343902 22.74455715400424 62-63 22.6657523029522 27.61787970987925 26.93811283337213 22.778255153796422 64-65 22.553076384924804 27.44727694727263 26.879275810328135 23.12037085747443 66-67 22.629499526551584 27.706193459031404 26.615652534401562 23.04865448001545 68-69 22.59575143024503 27.46584764887033 26.929633357883976 23.008767563000664 70-71 22.60090541648216 27.544265852639775 26.908896195868937 22.945932535009124 72-73 22.76356225371109 27.530855910757207 26.7115644063532 22.994017429178506 74-75 22.587922952952063 27.849366223110504 26.6008096581208 22.961901165816634 76-77 22.782682624022307 27.633358153037218 26.666449477903072 22.9175097450374 78-79 22.786056878877027 27.531917132047145 26.730661880568963 22.951364108506862 80-81 22.66790239184264 27.72000854173438 26.680319310129335 22.93176975629364 82-83 22.968126027747868 27.75571858639206 26.550115625928626 22.726039759931446 84-85 22.844475323543612 27.619613985091675 26.668472308906132 22.867438382458577 86-87 22.62415941397463 27.656683664856928 26.871508599199945 22.847648321968492 88-89 22.87443631248751 27.609509560948915 26.726178301716057 22.789875824847513 90-91 22.800268048627192 27.72171621784486 26.70255827839606 22.775457455131885 92-93 22.794360132414557 27.52584062513741 26.800049624050704 22.879749618397334 94-95 22.61739322963284 27.572295134995933 26.917885979705858 22.89242565566537 96-97 22.85399776938801 27.514139575382867 26.768286052236835 22.863576602992286 98-99 22.562378173380996 27.62903070668763 26.647822186627096 23.160768933304283 100-101 22.540935825171946 27.556474814354516 26.792342028127592 23.110247332345946 102-103 22.577538704576398 27.444131775361917 26.927769777846173 23.05055974221551 104-105 22.407241367939566 27.56143213141114 26.886852448256317 23.144474052392983 106-107 22.4355176782399 27.709564846647517 26.721802344640388 23.1331151304722 108-109 22.528856375253 27.752523706114836 26.5009554675196 23.217664451112565 110-111 22.42191403979068 27.872396300502338 26.657863198979697 23.047826460727283 112-113 22.458486941822635 27.87415534141421 26.769077124252792 22.898280592510357 114-115 22.26157869161197 28.06358320242859 26.57091583079963 23.103922275159814 116-117 22.32719288329534 27.961656335763763 26.780078941914574 22.93107183902632 118-119 22.227596251614496 27.9971576967922 26.588882825657674 23.186363225935626 120-121 22.15866266361832 28.04743047006507 26.80349157098711 22.9904152953295 122-123 22.211030501060247 28.075169635700313 26.666975463961396 23.046824399278044 124-125 22.295592657740006 28.116321144777682 26.468705188893104 23.119381008589208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 647.0 1 601.5 2 584.0 3 548.0 4 425.5 5 341.0 6 306.5 7 284.0 8 273.5 9 276.5 10 261.0 11 236.0 12 226.5 13 237.0 14 254.5 15 309.5 16 361.0 17 372.5 18 454.5 19 586.5 20 767.0 21 1060.5 22 1461.0 23 2083.5 24 2765.5 25 3674.0 26 5076.0 27 6740.0 28 8376.0 29 10617.5 30 13504.0 31 16462.5 32 19889.0 33 23431.0 34 26766.0 35 29343.5 36 33197.5 37 36948.0 38 37898.0 39 39009.5 40 39797.0 41 40577.0 42 41587.5 43 42590.0 44 44498.0 45 46513.0 46 47409.0 47 47810.0 48 47998.0 49 47521.5 50 46981.5 51 45737.5 52 44874.5 53 45024.5 54 43962.0 55 40991.0 56 37234.0 57 33589.0 58 31366.5 59 28336.5 60 23629.5 61 19000.5 62 15017.5 63 11375.5 64 8470.5 65 6677.0 66 4898.5 67 3693.0 68 2719.0 69 1800.5 70 1385.5 71 1024.5 72 809.0 73 728.0 74 578.0 75 440.5 76 317.5 77 192.0 78 133.0 79 88.0 80 62.0 81 45.5 82 29.0 83 22.0 84 21.0 85 18.0 86 14.0 87 12.0 88 12.5 89 10.0 90 9.5 91 6.5 92 3.5 93 5.0 94 5.5 95 3.0 96 1.0 97 1.5 98 5.5 99 5.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054552119251717615 2 0.2241738886084971 3 0.027629274786481438 4 0.111223529467171 5 0.02472506124358424 6 0.04450511023845163 7 0.04128692766388987 8 0.10941820753509977 9 0.13210247007286435 10-11 0.04858670765009093 12-13 0.03214257961665951 14-15 0.013108207071995453 16-17 0.03359468638810811 18-19 0.05945788537147638 20-21 0.1925415086682925 22-23 0.11361754333361329 24-25 0.07692241275781764 26-27 0.016718850936137914 28-29 0.006514857407039656 30-31 0.008516409983901239 32-33 0.00718204159932685 34-35 0.010047009013265976 36-37 0.004670289346010357 38-39 0.009772286110559485 40-41 0.006475611278081587 42-43 0.011302885139924224 44-45 0.011302885139924224 46-47 0.017425281257383178 48-49 0.018759649641957567 50-51 0.011734592558462997 52-53 0.015149005777815106 54-55 0.015423728680521597 56-57 0.017229050612592826 58-59 0.01934834157632862 60-61 0.01075343933451124 62-63 0.02083969447673529 64-65 0.033123732840611264 66-67 0.029591581234384948 68-69 0.03555699283601162 70-71 0.02692284446523617 72-73 0.01785698867592195 74-75 0.010988916108259661 76-77 0.01236253062179212 78-79 0.02527450704899722 80-81 0.020800448347777216 82-83 0.022880493182554938 84-85 0.01773925028904774 86-87 0.027158321238984595 88-89 0.02876741252626547 90-91 0.02837495123668477 92-93 0.03414413219352109 94-95 0.02692284446523617 96-97 0.02900288930001389 98-99 0.03363393251706618 100-101 0.021938586087561253 102-103 0.03053348832937863 104-105 0.025823952854410203 106-107 0.03339845574331776 108-109 0.022684262537764587 110-111 0.02123215576631599 112-113 0.027119075110026526 114-115 0.014442575456569842 116-117 0.012794238040330893 118-119 0.03108293413479162 120-121 0.02472506124358424 122-123 0.01978004899486739 124-125 0.020525725445070725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1274011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.23850552601807 #Duplication Level Percentage of deduplicated Percentage of total 1 73.2081146783833 38.97490617854474 2 14.042916666699359 14.952477931229705 3 5.175104320764148 8.265444599361663 4 2.604934918858479 5.547313682902584 5 1.353015810476385 3.6016269851418423 6 0.8451721903477294 2.6997422595758636 7 0.5748104254141768 2.1421433606877818 8 0.38365313099512544 1.6340095467646496 9 0.26404396807609654 1.2651575628177905 >10 1.414282823991692 13.80058562500449 >50 0.0939818535566351 3.395991748478538 >100 0.039674238189235704 3.60933776373423 >500 2.949742475747997E-4 0.1112627557561192 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.849225791614044E-5 0.0 24-25 0.0 0.0 0.0 1.9623064479035109E-4 0.0 26-27 0.0 0.0 0.0 3.53215160622632E-4 0.0 28-29 0.0 0.0 0.0 0.0011381377397840363 0.0 30-31 0.0 0.0 0.0 0.003767628379974741 0.0 32-33 0.0 0.0 0.0 0.009419070949936853 0.0 34-35 0.0 0.0 0.0 0.0176607580311316 0.0 36-37 0.0 0.0 0.0 0.032770517679988634 0.0 38-39 0.0 0.0 0.0 0.05616121053899849 0.0 40-41 0.0 0.0 0.0 0.08555656112859308 0.0 42-43 0.0 0.0 0.0 0.11212619043320662 0.0 44-45 0.0 0.0 0.0 0.14269892489154332 0.0 46-47 0.0 0.0 0.0 0.1786876251460937 0.0 48-49 0.0 0.0 0.0 0.21895415345707375 0.0 50-51 0.0 0.0 0.0 0.26589252369092575 0.0 52-53 0.0 0.0 0.0 0.3124776787641551 0.0 54-55 0.0 0.0 0.0 0.36098589415632987 0.0 56-57 0.0 0.0 0.0 0.41981584146447715 0.0 58-59 0.0 0.0 0.0 0.48637727617736426 0.0 60-61 0.0 0.0 0.0 0.5609056750687396 0.0 62-63 0.0 0.0 0.0 0.6383382875030121 0.0 64-65 0.0 0.0 0.0 0.719852497348924 0.0 66-67 0.0 0.0 0.0 0.8102755784683178 0.0 68-69 0.0 0.0 0.0 0.9167110802026042 0.0 70-71 0.0 0.0 0.0 1.041749247063016 0.0 72-73 0.0 0.0 0.0 1.1643934000569853 0.0 74-75 0.0 0.0 0.0 1.2930029646525814 0.0 76-77 0.0 0.0 0.0 1.4314240614876952 0.0 78-79 0.0 0.0 0.0 1.591705252152454 0.0 80-81 0.0 0.0 0.0 1.7748669359997677 0.0 82-83 0.0 0.0 0.0 1.9725496875615673 0.0 84-85 0.0 0.0 0.0 2.166778779775057 0.0 86-87 0.0 0.0 0.0 2.374901001639703 0.0 88-89 0.0 0.0 0.0 2.6031172415308816 0.0 90-91 0.0 0.0 0.0 2.8639470145862163 0.0 92-93 0.0 0.0 0.0 3.14106393115915 0.0 94-95 0.0 0.0 0.0 3.423832290302046 0.0 96-97 0.0 0.0 0.0 3.7099758165353363 0.0 98-99 0.0 0.0 0.0 4.024415801747395 0.0 100-101 0.0 0.0 0.0 4.392976198792632 0.0 102-103 0.0 0.0 0.0 4.790460992879968 0.0 104-105 0.0 0.0 0.0 5.184649112134824 0.0 106-107 7.849225791614044E-5 0.0 0.0 5.573499757851384 0.0 108-109 7.849225791614044E-5 0.0 0.0 5.994257506410856 0.0 110-111 7.849225791614044E-5 0.0 0.0 6.471411942283073 0.0 112-113 7.849225791614044E-5 0.0 0.0 6.971996317143258 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2655 0.0 55.07159 1 GGTATCA 1520 0.0 46.722862 1 ATCAACG 3040 0.0 45.88337 3 TCAACGC 3075 0.0 45.176155 4 CAACGCA 3185 0.0 43.588467 5 AACGCAG 3380 0.0 41.073746 6 TATCAAC 3630 0.0 39.772312 2 GTACATG 7170 0.0 36.95642 1 TACATGG 7300 0.0 35.14129 2 ACGCAGA 3960 0.0 35.057896 7 CGCAGAG 4070 0.0 33.96399 8 ACATGGG 7720 0.0 32.89314 3 CATGGGA 4845 0.0 29.410513 4 GCAGAGT 4900 0.0 27.10905 9 CATGGGG 3045 0.0 26.62871 4 CCCGTAC 115 9.941808E-4 25.917028 3 ATGGGGA 1635 0.0 22.229883 5 GAGTACA 5585 0.0 21.798824 1 AGTACAT 5375 0.0 21.75453 2 GTACTAG 385 9.094947E-12 21.701681 1 >>END_MODULE