FastQCFastQC Report
Fri 27 May 2016
SRR940009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274011
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59450.4666364733114549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40600.3186785671395302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.20737654541444303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.068.956221
GTATCAA58800.058.9086571
ATCAACG74100.046.330293
TCAACGC74200.046.2678534
CAACGCA76400.044.8790665
TATCAAC78600.043.907012
AACGCAG79850.043.1467256
ACGCAGA92200.037.3614437
CGCAGAG93600.036.7390568
GCAGAGT103900.033.039719
GTACATG71900.031.6748641
GTGGTAT14000.031.512581
TACATGG73750.030.6305662
TGGTATC14400.029.3882482
ACATGGG75850.028.9955643
GAGTACT63850.028.4659912-13
GTACTTT66200.027.09600814-15
CAGAGTA101650.026.39642710-11
AGTACTT66700.026.13471612-13
AGAGTAC100450.025.9121910-11
Produced by FastQC (version 0.11.5)