FastQCFastQC Report
Fri 27 May 2016
SRR940008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713163
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23150.32461022234748577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16100.055.239511
GGTATCA9050.048.1463851
TCAACGC19950.042.732974
CAACGCA20350.041.860635
ATCAACG20500.041.2956623
AACGCAG21150.039.9955866
TATCAAC22900.038.0545652
GTACATG45450.036.903011
TACATGG45450.035.064432
ACGCAGA24850.033.5610667
ACATGGG46200.032.647393
CGCAGAG26000.032.3057568
TATTCCG1352.307368E-630.8886155
AATAGCG1004.40612E-429.7854465
CATGGGA30050.027.576574
GCAGAGT29950.027.0315639
GTGTAAG3600.026.5281451
ATTCCGT1401.04686136E-425.5303866
GAGTACA31850.024.9247951
ATAGCGT1200.001278477124.8212076
Produced by FastQC (version 0.11.5)