##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713163 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32205120007628 34.0 31.0 34.0 28.0 34.0 2 31.318746205285468 34.0 31.0 34.0 28.0 34.0 3 31.513862889690014 34.0 31.0 34.0 28.0 34.0 4 34.56817726101887 37.0 35.0 37.0 32.0 37.0 5 34.73571960407368 37.0 35.0 37.0 32.0 37.0 6 34.627034773256604 37.0 37.0 37.0 31.0 37.0 7 34.59572916710486 37.0 36.0 37.0 31.0 37.0 8 34.5202541354501 37.0 37.0 37.0 31.0 37.0 9 36.06976806143897 39.0 38.0 39.0 31.0 39.0 10-11 36.177463637345184 39.0 38.5 39.0 31.0 39.0 12-13 36.14142839715465 39.0 39.0 39.0 31.0 39.0 14-15 37.62032312388612 41.0 39.0 41.0 31.0 41.0 16-17 37.69167427362328 41.0 39.0 41.0 31.0 41.0 18-19 37.722083170327124 41.0 39.5 41.0 31.0 41.0 20-21 37.755413559032085 41.0 40.0 41.0 31.0 41.0 22-23 37.81034139460404 41.0 39.0 41.0 31.0 41.0 24-25 37.912810956261055 41.0 39.0 41.0 31.0 41.0 26-27 37.97542777738049 41.0 39.0 41.0 31.0 41.0 28-29 37.9377176045308 41.0 39.0 41.0 31.0 41.0 30-31 37.88236139564167 41.0 39.0 41.0 31.0 41.0 32-33 37.84159582031036 41.0 39.0 41.0 30.5 41.0 34-35 37.751537867219696 41.0 39.0 41.0 30.0 41.0 36-37 37.609036082915125 41.0 38.5 41.0 30.0 41.0 38-39 37.51303488823733 41.0 38.0 41.0 30.0 41.0 40-41 37.42362825889734 41.0 38.0 41.0 30.0 41.0 42-43 37.256452592184395 40.5 38.0 41.0 30.0 41.0 44-45 37.11141492197436 40.0 38.0 41.0 30.0 41.0 46-47 36.9613748890506 40.0 38.0 41.0 30.0 41.0 48-49 36.82414609282871 40.0 37.0 41.0 30.0 41.0 50-51 36.67709555879932 40.0 37.0 41.0 30.0 41.0 52-53 36.80614600028325 40.0 37.0 41.0 30.0 41.0 54-55 36.61998939933788 40.0 36.5 41.0 30.0 41.0 56-57 36.519480819952804 40.0 36.0 41.0 30.0 41.0 58-59 36.3376107565872 40.0 36.0 41.0 30.0 41.0 60-61 36.10183506435415 39.5 35.0 41.0 30.0 41.0 62-63 35.82188433780216 39.0 35.0 41.0 29.5 41.0 64-65 35.52428336859876 39.0 35.0 41.0 29.0 41.0 66-67 35.198672673708536 38.0 35.0 41.0 29.0 41.0 68-69 34.83313982918351 37.0 35.0 40.0 28.0 41.0 70-71 34.45542463644356 37.0 35.0 39.5 28.0 41.0 72-73 34.070153667534626 36.0 35.0 39.0 27.5 41.0 74-75 33.68939639325091 36.0 34.0 39.0 27.0 41.0 76-77 33.31332668688645 35.0 34.0 37.5 26.0 39.5 78-79 32.95985560103371 35.0 34.0 37.0 26.0 39.0 80-81 32.63034257245539 35.0 34.0 37.0 26.0 39.0 82-83 32.32263101142375 35.0 34.0 36.0 25.5 38.0 84-85 32.067922760995735 35.0 34.0 36.0 25.0 37.0 86-87 31.840352065376358 35.0 34.0 35.5 25.0 37.0 88-89 31.656421463255946 35.0 33.0 35.0 25.0 36.0 90-91 31.492264040619048 35.0 33.0 35.0 24.5 36.0 92-93 31.33697836259032 35.0 33.0 35.0 24.0 36.0 94-95 31.21457997680755 35.0 33.0 35.0 23.5 36.0 96-97 31.10259225450563 35.0 33.0 35.0 23.0 35.5 98-99 31.027377331689948 35.0 33.0 35.0 21.5 35.0 100-101 30.926244771531895 35.0 33.0 35.0 20.0 35.0 102-103 30.23661491131761 34.0 31.0 35.0 20.0 35.0 104-105 31.09532252794943 35.0 33.0 35.0 23.0 35.0 106-107 31.19013675695458 35.0 34.0 35.0 23.0 35.0 108-109 31.17261201155977 35.0 34.0 35.0 23.0 35.0 110-111 31.116356989916753 35.0 34.0 35.0 22.0 35.0 112-113 31.060152447617163 35.0 34.0 35.0 20.0 35.0 114-115 30.9888643970593 35.0 33.5 35.0 19.5 35.0 116-117 30.9040345615238 35.0 33.0 35.0 18.5 35.0 118-119 30.806374559532674 35.0 33.0 35.0 17.0 35.0 120-121 30.69990941762262 35.0 33.0 35.0 5.0 35.0 122-123 30.55574812490272 35.0 33.0 35.0 2.0 35.0 124-125 29.343990083613424 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26344718206517115 1101 2 0.04098899253855848 1101 3 0.1495502576411134 1101 4 0.10026522973401342 1101 5 0.06553244606074315 1101 6 0.13216383650453878 1101 7 0.17868771928415583 1101 8 0.03303635078309952 1101 9 0.3454760591989867 1101 10-11 0.18988724634252208 1101 12-13 0.201357608273959 1101 14-15 0.20484729663728274 1101 16-17 0.1055874691303984 1101 18-19 0.18061649918576705 1101 20-21 0.034706501357462116 1101 22-23 0.05744683831038344 1101 24-25 0.14498625022324063 1101 26-27 0.08033762285568713 1101 28-29 0.06865369172048474 1101 30-31 0.07849549397632671 1101 32-33 0.134713984457008 1101 34-35 0.16557882211068886 1101 36-37 0.1554908212903996 1101 38-39 0.11287305315332219 1101 40-41 0.09398030254362766 1101 42-43 0.11150796871472579 1101 44-45 0.04997202371053078 1101 46-47 0.23061150435972166 1101 48-49 -0.014245514013083493 1101 50-51 0.052535814501638356 1101 52-53 0.13912438408804206 1101 54-55 0.15211532172607178 1101 56-57 0.013896704321624043 1101 58-59 0.0661120979492722 1101 60-61 0.0791139023327645 1101 62-63 0.16646180933028631 1101 64-65 0.09766107451680739 1101 66-67 0.07615801840786673 1101 68-69 0.19992838066524143 1101 70-71 0.2364421577223439 1101 72-73 0.2847680055022792 1101 74-75 0.344106153550257 1101 76-77 0.2252459725142586 1101 78-79 0.11483783018947236 1101 80-81 0.12957444368617388 1101 82-83 0.21606867307490774 1101 84-85 0.19895758809216346 1101 86-87 0.22161934597847832 1101 88-89 0.23092289574645974 1101 90-91 0.27118991013473703 1101 92-93 0.28704538720539574 1101 94-95 0.2618944979557689 1101 96-97 0.18628899981843006 1101 98-99 0.17523407281703385 1101 100-101 0.19630140372859373 1101 102-103 0.24326354776110293 1101 104-105 0.30184758297837533 1101 106-107 0.32374074204785686 1101 108-109 0.2548748079145646 1101 110-111 0.3012593857330472 1101 112-113 0.29332558224365 1101 114-115 0.22202936418817032 1101 116-117 0.2734691221706882 1101 118-119 0.31633312560171234 1101 120-121 0.1732092425402385 1101 122-123 0.18236373571289022 1101 124-125 0.2513677234794116 1102 1 0.1766228272007595 1102 2 0.3718278167947098 1102 3 0.34168193683394676 1102 4 0.3060409941734221 1102 5 0.2683508337885314 1102 6 0.21772056460090994 1102 7 0.33710684828652404 1102 8 0.16907310564771194 1102 9 0.34929696931944676 1102 10-11 0.33561848282970175 1102 12-13 0.38103304325799314 1102 14-15 0.3924618338554424 1102 16-17 0.4760928485188458 1102 18-19 0.4252866577361658 1102 20-21 0.15679867164470807 1102 22-23 0.22641368686386443 1102 24-25 0.3299677183587306 1102 26-27 0.44375327713346024 1102 28-29 0.33927425179461324 1102 30-31 0.27853410202739326 1102 32-33 0.4188782488449476 1102 34-35 0.4603648048142759 1102 36-37 0.38440310379837683 1102 38-39 0.5015493117757899 1102 40-41 0.4976557890050728 1102 42-43 0.4475936271106846 1102 44-45 0.5091761830845627 1102 46-47 0.42628740264038356 1102 48-49 0.3590911224535063 1102 50-51 0.3494716221820653 1102 52-53 0.3607172235815028 1102 54-55 0.42710631318082903 1102 56-57 0.36305955308101545 1102 58-59 0.25861184056225994 1102 60-61 0.41913114726223455 1102 62-63 0.5109363537552838 1102 64-65 0.5045288033338622 1102 66-67 0.4607694927206438 1102 68-69 0.4888118358284288 1102 70-71 0.5236049807430305 1102 72-73 0.500329623951778 1102 74-75 0.47619073092079134 1102 76-77 0.4914484846114533 1102 78-79 0.4424947075702974 1102 80-81 0.38795303423491845 1102 82-83 0.3907012531222662 1102 84-85 0.44765199249862775 1102 86-87 0.4729931491289001 1102 88-89 0.46638632105275235 1102 90-91 0.5250076029176043 1102 92-93 0.4920908160121513 1102 94-95 0.42611448663074114 1102 96-97 0.3907733249496985 1102 98-99 0.2748544269815554 1102 100-101 0.4388275285098153 1102 102-103 0.4932538957483352 1102 104-105 0.6419143749067189 1102 106-107 0.5908904383311864 1102 108-109 0.6098011952305278 1102 110-111 0.5635566934649532 1102 112-113 0.5712848893578304 1102 114-115 0.5495234442870043 1102 116-117 0.5184668056876234 1102 118-119 0.5258776792926447 1102 120-121 0.5359280214962752 1102 122-123 0.5076719566538976 1102 124-125 0.4472585399109974 1103 1 -0.0192083563716281 1103 2 0.23367772908446227 1103 3 0.28165459187470887 1103 4 0.14724417238000598 1103 5 0.10373159944738575 1103 6 0.2144868941850575 1103 7 0.2701813504018915 1103 8 0.04000747774554014 1103 9 0.13992358164015428 1103 10-11 0.22885318513080222 1103 12-13 0.331031753401426 1103 14-15 0.4525588310693536 1103 16-17 0.37141776338830823 1103 18-19 0.38648841710053006 1103 20-21 0.2232733646588443 1103 22-23 0.20534001025670534 1103 24-25 0.30411125359761115 1103 26-27 0.4131456257042956 1103 28-29 0.4189345035746115 1103 30-31 0.3878288084560424 1103 32-33 0.46393422861999056 1103 34-35 0.4566584019662727 1103 36-37 0.3690305932215523 1103 38-39 0.412937455414216 1103 40-41 0.3688455527033483 1103 42-43 0.3712547559931565 1103 44-45 0.320005818829074 1103 46-47 0.24816337003280609 1103 48-49 0.19539220077359687 1103 50-51 0.05314384383102322 1103 52-53 0.10166823482905585 1103 54-55 0.3197218843293115 1103 56-57 0.2530289834803554 1103 58-59 0.13187053419552797 1103 60-61 0.23402512105235473 1103 62-63 0.12513573978355907 1103 64-65 0.08899183604462024 1103 66-67 0.06099199297861446 1103 68-69 0.17455376132631528 1103 70-71 0.2608108934455444 1103 72-73 0.21132436133698462 1103 74-75 0.11613371926048188 1103 76-77 0.26119618866675864 1103 78-79 0.3528915964362582 1103 80-81 0.3368186053834066 1103 82-83 0.3717074702309233 1103 84-85 0.33410785360687356 1103 86-87 0.4845470393363094 1103 88-89 0.5190105342402376 1103 90-91 0.39840108656222384 1103 92-93 0.38158944876418843 1103 94-95 0.3896005819769357 1103 96-97 0.28462135404885913 1103 98-99 0.3494042670393398 1103 100-101 0.28458970475582035 1103 102-103 0.32782011698453317 1103 104-105 0.4032541489238497 1103 106-107 0.5394741758195778 1103 108-109 0.5640577219047636 1103 110-111 0.6450517662128625 1103 112-113 0.6193597784301623 1103 114-115 0.7373110823016553 1103 116-117 0.6785754116106446 1103 118-119 0.609484247242289 1103 120-121 0.6707064241379044 1103 122-123 0.6454899062978967 1103 124-125 0.6109090919696101 1104 1 0.03823898881692145 1104 2 0.15715609962299126 1104 3 0.20536880552081627 1104 4 -0.08618122804880102 1104 5 0.13102760146529846 1104 6 0.11107915045949568 1104 7 0.26018765636733576 1104 8 0.38114381271842035 1104 9 0.24467575719823031 1104 10-11 0.04483565454687266 1104 12-13 0.1415633462883008 1104 14-15 0.2468052681988766 1104 16-17 0.2634539596299561 1104 18-19 0.09514776884727638 1104 20-21 -0.162330116234088 1104 22-23 -0.03393984848966625 1104 24-25 0.061533374924387374 1104 26-27 0.0761901458203269 1104 28-29 0.18440051442087224 1104 30-31 0.2410593545526467 1104 32-33 0.23930754177423808 1104 34-35 0.4089127846122551 1104 36-37 0.27511017450544273 1104 38-39 0.1828371905636672 1104 40-41 0.09305982940910695 1104 42-43 0.2536037281207868 1104 44-45 0.15619638510476364 1104 46-47 0.10068134203432066 1104 48-49 0.06256334467573055 1104 50-51 0.17192111713156066 1104 52-53 0.03911368822797101 1104 54-55 0.0747578283323378 1104 56-57 -0.0533167095452427 1104 58-59 0.04507648702691114 1104 60-61 -0.05538905475796696 1104 62-63 0.02059544466437302 1104 64-65 -0.0996504895954402 1104 66-67 -0.08487444667329669 1104 68-69 0.048559310575910786 1104 70-71 -0.019097039458984 1104 72-73 -0.30608451746235943 1104 74-75 -0.03348098625092888 1104 76-77 -0.016935353772389306 1104 78-79 0.03159824292383462 1104 80-81 0.04129899383088542 1104 82-83 -0.05893258526157297 1104 84-85 0.010821184417821428 1104 86-87 0.06197042185617008 1104 88-89 0.1360959170123479 1104 90-91 0.16800002716002993 1104 92-93 0.06927515944649443 1104 94-95 -0.0759688467025974 1104 96-97 -0.050224149797777784 1104 98-99 0.04659685122398116 1104 100-101 -0.08969519876291088 1104 102-103 -0.18723852849408473 1104 104-105 -0.06996693822459221 1104 106-107 -0.06239238995163987 1104 108-109 -0.07242102699169095 1104 110-111 -0.22872866007040216 1104 112-113 -0.10751561569267665 1104 114-115 -0.2079007981372385 1104 116-117 -0.1869624872416722 1104 118-119 -0.04784706818210438 1104 120-121 -0.1165214734532185 1104 122-123 -0.03526996253802395 1104 124-125 -0.14382731867485887 1105 1 -0.19021033963240797 1105 2 -0.13949835703146363 1105 3 -0.1380133678613582 1105 4 -0.21923373110130484 1105 5 -0.44690890647120796 1105 6 -0.31026395088360914 1105 7 -0.44843261225293674 1105 8 -0.5399428783682723 1105 9 -0.5218443893219131 1105 10-11 -0.5141692294580125 1105 12-13 -0.44043299070803954 1105 14-15 -0.35439131299770565 1105 16-17 -0.35969011851412347 1105 18-19 -0.41145784775833505 1105 20-21 -0.38613964004360923 1105 22-23 -0.5070778216276395 1105 24-25 -0.497379933988924 1105 26-27 -0.44026895063876736 1105 28-29 -0.4690549190345621 1105 30-31 -0.29766469917140626 1105 32-33 -0.3708695034028153 1105 34-35 -0.26102006032058966 1105 36-37 -0.215372120976852 1105 38-39 -0.34022141749493784 1105 40-41 -0.2869096455603639 1105 42-43 -0.26354523402817165 1105 44-45 -0.3229916490832636 1105 46-47 -0.3625237861708044 1105 48-49 -0.38658195446956967 1105 50-51 -0.3196967094862657 1105 52-53 -0.21199131787703607 1105 54-55 -0.1895645159899999 1105 56-57 -0.2392568804854207 1105 58-59 -0.25487467292425237 1105 60-61 -0.3224647568336678 1105 62-63 -0.2634704894015556 1105 64-65 -0.2665064114513527 1105 66-67 -0.4014495382483858 1105 68-69 -0.3379364159198204 1105 70-71 -0.3391947195566658 1105 72-73 -0.30781223419007375 1105 74-75 -0.2274431352130719 1105 76-77 -0.376758308595349 1105 78-79 -0.44070334437775216 1105 80-81 -0.3774128498809617 1105 82-83 -0.3647994961284837 1105 84-85 -0.3819871183904837 1105 86-87 -0.34912847924272583 1105 88-89 -0.37442911351268293 1105 90-91 -0.45654209738209417 1105 92-93 -0.3700532898819553 1105 94-95 -0.4497783705121172 1105 96-97 -0.3857064452800749 1105 98-99 -0.26926395463682695 1105 100-101 -0.2523875064552179 1105 102-103 -0.2758832171387766 1105 104-105 -0.3149974632551178 1105 106-107 -0.4156280431872972 1105 108-109 -0.3358032003738636 1105 110-111 -0.36126223760397735 1105 112-113 -0.41319937637643633 1105 114-115 -0.45627076150720214 1105 116-117 -0.3209063211855039 1105 118-119 -0.31838430871934165 1105 120-121 -0.2775959545276976 1105 122-123 -0.2944946267626847 1105 124-125 -0.24144026128779927 1106 1 -0.08080863023069895 1106 2 -0.08443120196431053 1106 3 -0.1635729934209813 1106 4 -0.09237170423927665 1106 5 -0.07865493821724101 1106 6 -0.10733354795320338 1106 7 0.0117220479017206 1106 8 0.15537661695123006 1106 9 -0.012361279838806638 1106 10-11 0.019835654546866976 1106 12-13 0.032013081738035964 1106 14-15 0.017810559204164633 1106 16-17 -0.19292170174570344 1106 18-19 -0.07977286607336254 1106 20-21 -0.22820313210709742 1106 22-23 -0.18023614478596528 1106 24-25 -0.11836080496979662 1106 26-27 -0.0639156742854965 1106 28-29 -0.14671059669023379 1106 30-31 -0.06616286766957558 1106 32-33 -0.15135383388714274 1106 34-35 -0.1610607603612877 1106 36-37 -0.28153003713477176 1106 38-39 -0.30094587821940166 1106 40-41 -0.24500895365967068 1106 42-43 -0.30324812373105914 1106 44-45 -0.24885652494813826 1106 46-47 -0.3062763299233495 1106 48-49 -0.21574353363115506 1106 50-51 -0.3369942267837871 1106 52-53 -0.3439815498672658 1106 54-55 -0.23995116637665603 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-1.514523373033171 2216 98-99 -1.6093504638722713 2216 100-101 -1.648950517911846 2216 102-103 -1.6192547392337033 2216 104-105 -1.6137933482212148 2216 106-107 -1.6779614544568773 2216 108-109 -1.6470377105445486 2216 110-111 -1.499162635078843 2216 112-113 -1.5881116980972685 2216 114-115 -1.5466495316732072 2216 116-117 -1.5450003123007718 2216 118-119 -1.6045779023597468 2216 120-121 -1.6215636889209648 2216 122-123 -1.6102767170128587 2216 124-125 -1.5811643669693538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14165.0 3 2775.0 4 2167.0 5 1580.0 6 1599.0 7 1767.0 8 1719.0 9 1702.0 10 2084.0 11 2503.0 12 2951.0 13 2835.0 14 2783.0 15 2654.0 16 2179.0 17 2016.0 18 1767.0 19 1616.0 20 1528.0 21 1342.0 22 1484.0 23 2255.0 24 4596.0 25 6940.0 26 8162.0 27 5661.0 28 5384.0 29 6570.0 30 7034.0 31 8280.0 32 11276.0 33 15153.0 34 23226.0 35 40880.0 36 96619.0 37 256933.0 38 158084.0 39 894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44770223141261 19.256139681435197 13.172853812031615 23.123304275120578 2 14.675878697950479 20.987440949418136 41.06744904296606 23.269231309665326 3 19.248009784175892 26.561877619258667 27.81042598192396 26.37968661464148 4 12.071272051422788 17.46805377550118 37.09173195352409 33.36894221955195 5 13.357195151712355 40.25770202834806 32.59634723433858 13.788755585601 6 30.93869718364192 37.56429985228744 17.73757103318833 13.759431930882304 7 28.562450903377844 33.94947256200202 19.91779822691056 17.57027830770957 8 25.1401227662122 38.256427820457574 18.990021209666892 17.613428203663332 9 25.920902622164565 19.391606153016696 19.16050297934954 35.5269882454692 10-11 24.336114341669813 29.360447580073824 26.19572369113271 20.107714387123657 12-13 24.786407508244228 26.571032215260278 26.807312991725613 21.835247284769885 14-15 22.189507607258204 27.651181021752453 24.637166950182944 25.522144420806402 16-17 22.21312050271415 30.76914984640848 25.232070469751587 21.785659181125776 18-19 22.037525876467797 29.612079215838676 26.911877254874177 21.43851765281935 20-21 23.347415419673805 28.504423423562653 26.652654826849343 21.495506329914203 22-23 22.923874157473715 28.597849561988102 26.327459250259523 22.150817030278656 24-25 22.28053656051742 28.61391807334416 26.843251526424584 22.26229383971384 26-27 22.286656321370796 28.843180414153096 26.656714052609548 22.213449211866564 28-29 22.20117989715515 28.978647142879176 26.66337593060286 22.156797029362806 30-31 22.386629121780615 28.504898317491744 26.872523145356485 22.235949415371152 32-33 22.043520787539045 28.693780390754547 26.80227034493397 22.46042847677244 34-35 22.50238749332133 28.886074848792088 26.52305245754048 22.0884852003461 36-37 22.596791608823075 28.576626982457615 26.419306437816388 22.40727497090292 38-39 22.26283565093985 28.62435702546095 26.79605156136936 22.31675576222984 40-41 22.495812360076034 28.61933739581734 26.61866569066084 22.266184553445783 42-43 22.236292600290007 28.60989339234478 26.60689794441593 22.546916062949283 44-45 22.380291269624237 28.64448137318833 26.736083356121643 22.239144001065792 46-47 22.366419761397367 28.604336584965633 26.509814151367443 22.51942950226955 48-49 22.25733817928866 28.651839427124493 26.572837639693454 22.517984753893394 50-51 22.311000292394993 28.871937302659674 26.60121319381581 22.21584921112952 52-53 22.52007208420108 28.56869385951995 26.6112712203126 22.29996283596637 54-55 22.329849962380155 28.540314940863563 26.69837999760187 22.431455099154412 56-57 22.333264146392086 28.909997139026483 26.532934853950106 22.22380386063132 58-59 22.516461554643946 28.64008975842362 26.513376108832087 22.330072578100346 60-61 22.227784871324005 28.703409600057217 26.734161700599994 22.334643828018784 62-63 22.112375308988273 28.83729247383461 26.771199663400008 22.27913255377711 64-65 22.08425238544432 28.680317147496027 26.689694109065915 22.545736357993736 66-67 22.249713688997172 28.913098211161113 26.395174406005484 22.44201369383623 68-69 22.279368290081013 28.737302573116068 26.70094495578213 22.282384181020788 70-71 22.118321293246307 28.603137178446925 26.83152270341272 22.44701882489405 72-73 22.265830436117977 28.690005203066864 26.5622874420966 22.48187691871856 74-75 22.14377568950599 28.881911362351932 26.498177631495913 22.476135316646168 76-77 22.302570409647203 28.79576819894626 26.580860131514548 22.32080125989199 78-79 22.316428159674153 28.753452074891616 26.540267077339102 22.38985268809513 80-81 22.15631219241272 29.032592856471656 26.46355095096976 22.34754400014586 82-83 22.351496570111795 28.971861031673345 26.457194069540265 22.219448328674595 84-85 22.465220876099437 28.76126083483922 26.433115882729542 22.3404024063318 86-87 22.236832508611887 28.76678897229609 26.63492611169085 22.361452407401174 88-89 22.367339553515826 28.654123442382897 26.56343800669895 22.415098997402335 90-91 22.305927170616897 28.857191347548365 26.561320926735803 22.275560555098927 92-93 22.284042268921176 28.849092769579393 26.574196095868842 22.292668865630585 94-95 22.09870863588357 28.894394831738357 26.650462083097814 22.35643444928026 96-97 22.201744143234343 28.7681243271864 26.729433381372665 22.300698148206592 98-99 22.11550802589101 28.63553189982207 26.47698907928965 22.771970994997275 100-101 21.947882553418278 28.986612997103805 26.62540935898065 22.440095090497262 102-103 22.107183282908828 28.767636303960884 26.651630485856593 22.473549927273698 104-105 21.921548111286278 28.850010940121066 26.724892422142805 22.503548526449848 106-107 21.774026697226407 29.039633451415693 26.609418581735273 22.57692126962263 108-109 21.890964886628925 29.111467824606358 26.387237978626644 22.610329310138074 110-111 21.707378476422495 29.231507174969966 26.510537524921123 22.550576823686413 112-113 21.80939510334782 29.362170062268877 26.486468669547158 22.341966164836144 114-115 21.728837947036492 29.50248016638221 26.401886525200652 22.36679536138065 116-117 21.753218295781 29.375525455722183 26.545514720473335 22.32574152802349 118-119 21.779447883309558 29.481126451582135 26.246582759293002 22.492842905815305 120-121 21.56653737483108 29.670056866434358 26.39818565989679 22.365220098837778 122-123 21.75896318237152 29.502347733889835 26.30583932187796 22.432849761860684 124-125 21.891913118724744 29.569829134987984 26.2101711174597 22.328086628827563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2065.0 1 1910.0 2 1877.0 3 1667.0 4 1040.5 5 621.5 6 461.0 7 416.5 8 421.0 9 400.5 10 322.0 11 270.0 12 256.5 13 258.5 14 281.5 15 303.5 16 327.5 17 368.5 18 422.5 19 494.5 20 615.5 21 812.5 22 1126.0 23 1573.5 24 2108.0 25 2726.5 26 3602.0 27 4605.0 28 5684.0 29 6965.5 30 8262.0 31 9538.5 32 11050.5 33 12711.5 34 14501.0 35 15996.0 36 17714.0 37 19227.5 38 20180.5 39 21235.0 40 22063.0 41 23140.5 42 24132.0 43 24975.5 44 25857.0 45 26946.0 46 27298.5 47 27044.0 48 27246.5 49 26964.5 50 26413.5 51 25538.0 52 24168.0 53 23702.5 54 23079.5 55 21319.5 56 19068.0 57 17110.5 58 15956.5 59 14281.5 60 11919.0 61 9511.0 62 7653.5 63 6003.5 64 4518.0 65 3445.0 66 2540.5 67 2008.0 68 1510.0 69 1038.5 70 779.0 71 601.5 72 441.5 73 383.0 74 297.5 75 214.0 76 159.5 77 98.0 78 72.5 79 56.5 80 41.0 81 31.0 82 27.0 83 23.0 84 16.5 85 11.5 86 10.0 87 8.0 88 4.5 89 4.5 90 6.0 91 4.0 92 4.5 93 4.5 94 4.0 95 3.0 96 1.5 97 3.0 98 3.0 99 2.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047815155861983864 2 0.2082272916570265 3 0.024538569723892012 4 0.11133499634725863 5 0.023697247333358575 6 0.0409443563392941 7 0.03968237275349394 8 0.10544573961352455 9 0.12998430933741656 10-11 0.04935758024462851 12-13 0.033652895621337615 14-15 0.013180717451690568 16-17 0.03267135283238194 18-19 0.056298489966529384 20-21 0.19406503141638026 22-23 0.11168554734331422 24-25 0.07712121913223205 26-27 0.01647589681461321 28-29 0.007011019921112005 30-31 0.008553444303756645 32-33 0.007782232112434324 34-35 0.010095868686401286 36-37 0.004627273147933922 38-39 0.009955648287979046 40-41 0.0073615709171676045 42-43 0.011357852272201447 44-45 0.010937191076934727 46-47 0.019140084384635773 48-49 0.01801832119725785 50-51 0.012129064463523766 52-53 0.015143803029601928 54-55 0.01521391322881305 56-57 0.016686227412246568 58-59 0.01935041498226913 60-61 0.010306199284034645 62-63 0.02110316996254713 64-65 0.03442410781265994 66-67 0.030077275461570498 68-69 0.03821005857006042 70-71 0.027693528688392414 72-73 0.01710688860751329 74-75 0.011217631873779206 76-77 0.012058954264312647 78-79 0.026080994106536658 80-81 0.02159394135702497 82-83 0.023276586138091852 84-85 0.01787810079883561 86-87 0.027342977692336814 88-89 0.030147385660781616 90-91 0.029306063270248175 92-93 0.03526543020319338 94-95 0.027483198090759057 96-97 0.028815291875770335 98-99 0.03526543020319338 100-101 0.022154822950713932 102-103 0.030287606059203855 104-105 0.026782096098647852 106-107 0.03400344661739322 108-109 0.024188018727836415 110-111 0.02292603514203625 112-113 0.028955512274192578 114-115 0.01486336223275745 116-117 0.012830166455634966 118-119 0.03281157323080418 120-121 0.024748900321525373 122-123 0.01892975378700241 124-125 0.02019173737280257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 713163.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.39364759554785 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94416381637913 51.62446828333497 2 12.485022179077717 16.328822812024143 3 4.0486026115580875 7.942586773039329 4 1.7020275973182044 4.452071715876947 5 0.8486914223913579 2.7749513896612346 6 0.5031120658373235 1.9740199880660403 7 0.329826837953616 1.509800600608484 8 0.20542639357817383 1.074686495078033 9 0.14546045184564954 0.8560970574375206 >10 0.7434233832682219 8.423510544060512 >50 0.03200141032379905 1.4050442570490465 >100 0.011812292558010536 1.2172390157090447 >500 2.147689553560261E-4 0.09157030548422379 >1k 2.147689553560261E-4 0.3251307625705185 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2315 0.32461022234748577 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.011019921112004E-5 0.0 16-17 0.0 0.0 0.0 2.804407968444802E-4 0.0 18-19 0.0 0.0 0.0 4.907713944778402E-4 0.0 20-21 0.0 0.0 0.0 5.608815936889604E-4 0.0 22-23 0.0 0.0 0.0 7.011019921112005E-4 0.0 24-25 0.0 0.0 0.0 0.0010516529881668007 0.0 26-27 0.0 0.0 0.0 0.0014022039842224007 0.0 28-29 0.0 0.0 0.0 0.002243526374755841 0.0 30-31 0.0 0.0 0.0 0.006590358725845283 0.0 32-33 0.0 0.0 0.0 0.013531268447746167 0.0 34-35 0.0 0.0 0.0 0.02089283936491377 0.0 36-37 0.0 0.0 0.0 0.035405650601615615 0.0 38-39 0.0 0.0 0.0 0.05791102454838515 0.0 40-41 0.0 0.0 0.0 0.0867964266233666 0.0 42-43 0.0 0.0 0.0 0.11343830232359223 0.0 44-45 0.0 0.0 0.0 0.14190304320330696 0.0 46-47 0.0 0.0 0.0 0.17920166918362282 0.0 48-49 0.0 0.0 0.0 0.22302054369057284 0.0 50-51 0.0 0.0 0.0 0.26796118138490077 0.0 52-53 0.0 0.0 0.0 0.31234093748553976 0.0 54-55 0.0 0.0 0.0 0.35910444035935685 0.0 56-57 0.0 0.0 0.0 0.41442138753693053 0.0 58-59 0.0 0.0 0.0 0.48481202754489505 0.0 60-61 0.0 0.0 0.0 0.5650882056416275 0.0 62-63 0.0 0.0 0.0 0.6409474411880594 0.0 64-65 0.0 0.0 0.0 0.7199616356989917 0.0 66-67 0.0 0.0 0.0 0.8037433237562801 0.0 68-69 0.0 0.0 0.0 0.9044916800226597 0.0 70-71 0.0 0.0 0.0 1.02907750402082 0.0 72-73 0.0 0.0 0.0 1.151279581245802 0.0 74-75 0.0 0.0 0.0 1.2729207768770954 0.0 76-77 0.0 0.0 0.0 1.4113884203190574 0.0 78-79 0.0 0.0 0.0 1.5698374705361888 0.0 80-81 0.0 0.0 0.0 1.7495299111142895 0.0 82-83 0.0 0.0 0.0 1.9405100937653805 0.0 84-85 0.0 0.0 0.0 2.1234276035071926 0.0 86-87 0.0 0.0 0.0 2.310271284404828 0.0 88-89 0.0 0.0 0.0 2.533011387298556 0.0 90-91 0.0 0.0 0.0 2.7805003905138097 0.0 92-93 0.0 0.0 0.0 3.048531682097922 0.0 94-95 0.0 0.0 0.0 3.3078693089798543 0.0 96-97 0.0 0.0 0.0 3.5754098291694887 0.0 98-99 0.0 0.0 0.0 3.8725368534262152 0.0 100-101 0.0 0.0 0.0 4.229397767410816 0.0 102-103 0.0 0.0 0.0 4.610516810322465 0.0 104-105 0.0 0.0 0.0 4.982381306938246 0.0 106-107 0.0 0.0 0.0 5.340153653512591 0.0 108-109 0.0 0.0 0.0 5.723516222798995 0.0 110-111 0.0 0.0 0.0 6.167734445000653 0.0 112-113 0.0 0.0 0.0 6.642240273261512 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1610 0.0 55.23951 1 GGTATCA 905 0.0 48.146385 1 TCAACGC 1995 0.0 42.73297 4 CAACGCA 2035 0.0 41.86063 5 ATCAACG 2050 0.0 41.295662 3 AACGCAG 2115 0.0 39.995586 6 TATCAAC 2290 0.0 38.054565 2 GTACATG 4545 0.0 36.90301 1 TACATGG 4545 0.0 35.06443 2 ACGCAGA 2485 0.0 33.561066 7 ACATGGG 4620 0.0 32.64739 3 CGCAGAG 2600 0.0 32.305756 8 TATTCCG 135 2.307368E-6 30.888615 5 AATAGCG 100 4.40612E-4 29.785446 5 CATGGGA 3005 0.0 27.57657 4 GCAGAGT 2995 0.0 27.031563 9 GTGTAAG 360 0.0 26.528145 1 ATTCCGT 140 1.04686136E-4 25.530386 6 GAGTACA 3185 0.0 24.924795 1 ATAGCGT 120 0.0012784771 24.821207 6 >>END_MODULE