FastQCFastQC Report
Fri 27 May 2016
SRR940008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713163
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62940.8825471876695791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.6420692043754372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.43706698188212234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.17331241244988874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.14877384272599672No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.1431650267891071No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.11119477594883638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.074.3120961
GTATCAA41700.064.234911
TAACGCA1200.054.6739734
TATCACG708.9848254E-751.123722
ATCAACG53200.050.2268073
TCAACGC53950.049.4180154
TATCAAC54150.049.345642
ATAACGC1350.048.599093
CAACGCA54950.048.531725
GTGGTAT8050.047.42911
AACGCAG56350.047.302736
ACGCAGA64100.041.580697
TGGTATC8750.041.5806242
TATAACG1750.040.8989752
CGCAGAG65400.040.4812558
GCAGAGT69350.038.0039679
ATCACGC1156.5277527E-736.305253
ACTGTCG1209.2894516E-734.7047128
TACATGG40150.034.018812
GTACATG40650.033.7542231
Produced by FastQC (version 0.11.5)