##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940008_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713163 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.125866036235756 34.0 33.0 34.0 31.0 34.0 2 33.16630559914073 34.0 33.0 34.0 31.0 34.0 3 33.227015422841625 34.0 33.0 34.0 31.0 34.0 4 36.459570953624905 37.0 37.0 37.0 35.0 37.0 5 36.47910365512512 37.0 37.0 37.0 35.0 37.0 6 36.558290320726115 37.0 37.0 37.0 35.0 37.0 7 36.54767283215759 37.0 37.0 37.0 35.0 37.0 8 36.553343345069784 37.0 37.0 37.0 35.0 37.0 9 38.427464688998164 39.0 39.0 39.0 37.0 39.0 10-11 38.4105849574361 39.0 39.0 39.0 37.0 39.0 12-13 38.39201557007304 39.0 39.0 39.0 37.0 39.0 14-15 39.982346251838635 41.0 40.0 41.0 38.0 41.0 16-17 39.99622106026252 41.0 40.0 41.0 38.0 41.0 18-19 40.04988691224867 41.0 40.0 41.0 38.0 41.0 20-21 40.049781045847865 41.0 40.0 41.0 38.0 41.0 22-23 39.99934727404535 41.0 40.0 41.0 38.0 41.0 24-25 39.96457682184858 41.0 40.0 41.0 38.0 41.0 26-27 39.867094198661455 41.0 40.0 41.0 37.5 41.0 28-29 39.77374036510587 41.0 40.0 41.0 37.0 41.0 30-31 39.58313036430662 41.0 40.0 41.0 36.5 41.0 32-33 39.359940855035944 41.0 39.5 41.0 35.0 41.0 34-35 39.22665715972366 41.0 39.0 41.0 35.0 41.0 36-37 39.10984795902199 41.0 39.0 41.0 35.0 41.0 38-39 38.979635791537135 41.0 39.0 41.0 35.0 41.0 40-41 38.83020922285648 41.0 39.0 41.0 35.0 41.0 42-43 38.6340471953817 41.0 39.0 41.0 35.0 41.0 44-45 38.36804349075877 41.0 38.5 41.0 35.0 41.0 46-47 37.98497818871702 41.0 38.0 41.0 34.0 41.0 48-49 37.640015676640544 41.0 38.0 41.0 33.0 41.0 50-51 37.63011821981791 41.0 38.0 41.0 33.0 41.0 52-53 37.52662855476238 41.0 38.0 41.0 33.0 41.0 54-55 37.376670550771706 41.0 37.5 41.0 33.0 41.0 56-57 37.20503797869492 40.5 37.0 41.0 33.0 41.0 58-59 37.00472192191687 40.0 36.5 41.0 33.0 41.0 60-61 36.7852293234506 40.0 36.0 41.0 33.0 41.0 62-63 36.534543295151316 39.5 35.5 41.0 32.0 41.0 64-65 36.24532259805963 39.0 35.0 41.0 32.0 41.0 66-67 35.940099247998006 39.0 35.0 41.0 31.5 41.0 68-69 35.59361183908868 37.5 35.0 41.0 31.0 41.0 70-71 35.23940179173625 37.0 35.0 40.0 31.0 41.0 72-73 34.87737866378373 36.5 35.0 39.0 31.0 41.0 74-75 34.515453690110114 36.0 35.0 39.0 30.5 41.0 76-77 34.149817783592255 35.5 35.0 38.0 30.0 40.5 78-79 33.80855078011619 35.0 35.0 37.0 30.0 39.0 80-81 33.495385346687925 35.0 35.0 37.0 29.5 39.0 82-83 33.20178837096148 35.0 35.0 36.0 29.0 38.0 84-85 32.9450139729627 35.0 35.0 36.0 29.0 37.0 86-87 32.72540639376973 35.0 35.0 36.0 29.0 37.0 88-89 32.60744037478108 35.0 35.0 35.0 29.0 36.5 90-91 32.583438849183146 35.0 35.0 35.0 29.0 36.0 92-93 32.488501226227385 35.0 35.0 35.0 29.0 36.0 94-95 32.4059632931041 35.0 35.0 35.0 29.0 36.0 96-97 32.33141722158889 35.0 35.0 35.0 29.0 36.0 98-99 32.26983031929586 35.0 35.0 35.0 29.0 35.5 100-101 32.207361430696764 35.0 35.0 35.0 29.0 35.0 102-103 31.738347334340116 35.0 33.5 35.0 26.5 35.0 104-105 32.10267778894867 35.0 34.5 35.0 29.0 35.0 106-107 32.14191916854912 35.0 35.0 35.0 29.0 35.0 108-109 32.122657793519856 35.0 35.0 35.0 29.0 35.0 110-111 32.07910463666792 35.0 35.0 35.0 29.0 35.0 112-113 32.04191608370036 35.0 35.0 35.0 29.0 35.0 114-115 31.981099692496667 35.0 35.0 35.0 28.0 35.0 116-117 31.92266073814822 35.0 35.0 35.0 27.0 35.0 118-119 31.856835674312883 35.0 35.0 35.0 27.0 35.0 120-121 31.775340980953864 35.0 34.5 35.0 27.0 35.0 122-123 31.672596587315944 35.0 34.0 35.0 25.0 35.0 124-125 30.653060380305767 34.5 32.5 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17586061069072656 1101 2 0.19872987705685574 1101 3 0.18917839541012427 1101 4 0.07813970165551609 1101 5 0.10472384286605063 1101 6 0.16721676520391782 1101 7 0.1894746614675782 1101 8 0.14743699688109757 1101 9 0.23746404076410954 1101 10-11 0.2150189007562986 1101 12-13 0.23884749576777864 1101 14-15 0.3785167760773973 1101 16-17 0.32182084660024657 1101 18-19 0.303579140967706 1101 20-21 0.3333150145447803 1101 22-23 0.3305789741481746 1101 24-25 0.3797007313881693 1101 26-27 0.3008900780733228 1101 28-29 0.3403948196321096 1101 30-31 0.40232515923509027 1101 32-33 0.42159707150691617 1101 34-35 0.4077464994735749 1101 36-37 0.41394811829695755 1101 38-39 0.5221087310782409 1101 40-41 0.45503933390671136 1101 42-43 0.5234433921182955 1101 44-45 0.6135802861159405 1101 46-47 0.6993200955768515 1101 48-49 0.7486549263183591 1101 50-51 0.691849989189059 1101 52-53 0.6812921950406761 1101 54-55 0.6398732165337719 1101 56-57 0.6290269246816536 1101 58-59 0.6424725550921195 1101 60-61 0.5461781900931371 1101 62-63 0.6129666769190791 1101 64-65 0.6730939783438004 1101 66-67 0.6613141081051452 1101 68-69 0.6634870564246 1101 70-71 0.621760530297621 1101 72-73 0.5304944154342337 1101 74-75 0.6086256456554153 1101 76-77 0.6529152079142264 1101 78-79 0.6901394379797878 1101 80-81 0.6154212622313935 1101 82-83 0.6437617915081546 1101 84-85 0.6048128160330606 1101 86-87 0.7107679200526462 1101 88-89 0.4280703341301404 1101 90-91 0.5513646582415745 1101 92-93 0.522973704047331 1101 94-95 0.521792530614789 1101 96-97 0.5009886449399588 1101 98-99 0.5064670171847965 1101 100-101 0.4923202897830379 1101 102-103 0.42484115922881927 1101 104-105 0.5654073459778601 1101 106-107 0.48108538393948663 1101 108-109 0.47358457491844064 1101 110-111 0.4651790277061778 1101 112-113 0.39694142565424784 1101 114-115 0.421751725826212 1101 116-117 0.5094645963447455 1101 118-119 0.45449464055729294 1101 120-121 0.4967449311160088 1101 122-123 0.6209073749532408 1101 124-125 0.6373631743329469 1102 1 0.22354026682168637 1102 2 0.23301253946805645 1102 3 0.14975571446706226 1102 4 0.037928129535679034 1102 5 0.08172760071635565 1102 6 0.13110150730213377 1102 7 0.1420382360583332 1102 8 0.1657783950073224 1102 9 0.12273893008769221 1102 10-11 0.18483512643322086 1102 12-13 0.17343837911997895 1102 14-15 0.2730131983979973 1102 16-17 0.27186781972903873 1102 18-19 0.23611285866564202 1102 20-21 0.2500187878383002 1102 22-23 0.21699730525082117 1102 24-25 0.17358843343697572 1102 26-27 0.13790619049996877 1102 28-29 0.149386634725289 1102 30-31 0.03872354284468571 1102 32-33 -0.029020657310127262 1102 34-35 0.01228551934384825 1102 36-37 -4.40828767267476E-5 1102 38-39 0.008586261192512268 1102 40-41 0.06979726142653675 1102 42-43 0.10875688949295181 1102 44-45 0.2658954822448507 1102 46-47 0.3409492266383154 1102 48-49 0.3250216513260824 1102 50-51 0.23784826469611886 1102 52-53 0.25300021521981364 1102 54-55 0.367182621455008 1102 56-57 0.4119415751501023 1102 58-59 0.3174390947811929 1102 60-61 0.2570341305852608 1102 62-63 0.17315276772522026 1102 64-65 0.13253107295926014 1102 66-67 0.2645970793807564 1102 68-69 0.1729247945075798 1102 70-71 0.2718982323464232 1102 72-73 0.33421301421936533 1102 74-75 0.1573091111140812 1102 76-77 0.14309460665818108 1102 78-79 0.2094421765775465 1102 80-81 0.15444370637841587 1102 82-83 0.14604867506627528 1102 84-85 0.17997883065264375 1102 86-87 0.18607549752614716 1102 88-89 0.11906243234902547 1102 90-91 0.03188367914139434 1102 92-93 0.036028373047642503 1102 94-95 0.09369101717919648 1102 96-97 0.11403623579757038 1102 98-99 0.15458165735776674 1102 100-101 0.21689238388372445 1102 102-103 0.25270652008539685 1102 104-105 0.1966547721077916 1102 106-107 0.19741723300774794 1102 108-109 0.15801330445616912 1102 110-111 0.09428949820962629 1102 112-113 0.0700187447111773 1102 114-115 -0.014949730984245235 1102 116-117 -0.011202293390660145 1102 118-119 0.05157329802666055 1102 120-121 0.11587843775659579 1102 122-123 0.1287428789169951 1102 124-125 0.016244570400072433 1103 1 0.15842443770188197 1103 2 0.09859271695232508 1103 3 0.09709216065424187 1103 4 0.013526622983206948 1103 5 0.08676191090106755 1103 6 0.06900910197760624 1103 7 0.054972917721734404 1103 8 0.079817193892886 1103 9 0.10393540104012544 1103 10-11 0.06098051906556634 1103 12-13 0.14042901476129543 1103 14-15 0.17394382991177082 1103 16-17 0.16029329181654361 1103 18-19 0.15460424242376547 1103 20-21 0.16396149240955538 1103 22-23 0.13557188475758153 1103 24-25 0.11607695371351667 1103 26-27 0.17022354680994312 1103 28-29 0.18009425003846502 1103 30-31 0.287705846012571 1103 32-33 0.3031380466419904 1103 34-35 0.22367108326913865 1103 36-37 0.18423708005795447 1103 38-39 0.1277464355811233 1103 40-41 0.19755690955366845 1103 42-43 0.2724603256708491 1103 44-45 0.23052116586882931 1103 46-47 0.19128652413083813 1103 48-49 0.2126751442576662 1103 50-51 0.26637021805490235 1103 52-53 0.22885410026779596 1103 54-55 0.22162295269017562 1103 56-57 0.13089214784641712 1103 58-59 0.31876506733304666 1103 60-61 0.3296085553092283 1103 62-63 0.4231927532788191 1103 64-65 0.27453525209454455 1103 66-67 0.18317488352892752 1103 68-69 0.29973768791383293 1103 70-71 0.2037667185707619 1103 72-73 0.17731421636568712 1103 74-75 0.12250491134109609 1103 76-77 0.24891056992305494 1103 78-79 0.26617909743195156 1103 80-81 0.3375852810353095 1103 82-83 0.29946550511477454 1103 84-85 0.21956581857958213 1103 86-87 0.2054865490172162 1103 88-89 0.27489064925130435 1103 90-91 0.3172418723103192 1103 92-93 0.18130749800894108 1103 94-95 0.2395531573092029 1103 96-97 0.22250349923787383 1103 98-99 0.1603382872205259 1103 100-101 0.251021343743389 1103 102-103 0.2328407941541215 1103 104-105 0.21618126576169772 1103 106-107 0.1834100356080981 1103 108-109 0.2581455147543821 1103 110-111 0.2961417322411961 1103 112-113 0.18453737024001526 1103 114-115 0.251265700859733 1103 116-117 0.2862811964452696 1103 118-119 0.28663933868293867 1103 120-121 0.2822821886594902 1103 122-123 0.25495598901915173 1103 124-125 0.2061604717518435 1104 1 0.024272590054010834 1104 2 0.05914890310442189 1104 3 0.02649813870948492 1104 4 -0.13313247652492777 1104 5 0.004732651221409867 1104 6 0.00880352750415625 1104 7 0.08193722595732567 1104 8 0.0895157687446968 1104 9 0.17016317251194124 1104 10-11 0.1589260355241393 1104 12-13 0.16218837911998207 1104 14-15 0.2740106731454759 1104 16-17 0.2219309510421681 1104 18-19 0.19979972735250584 1104 20-21 0.2052081817776923 1104 22-23 0.2523129618164788 1104 24-25 0.2343081304066743 1104 26-27 0.202830432924209 1104 28-29 0.22501794785659968 1104 30-31 0.17046596708711093 1104 32-33 0.0944641911747155 1104 34-35 0.19352289308122295 1104 36-37 0.16888268480004598 1104 38-39 0.20481100866726365 1104 40-41 0.1988250392043085 1104 42-43 0.27481749555355606 1104 44-45 0.30556719941656496 1104 46-47 0.10033053976962947 1104 48-49 0.10663781294224606 1104 50-51 0.09680028489813708 1104 52-53 0.1158284980480957 1104 54-55 0.14911443963682558 1104 56-57 0.05184056504908341 1104 58-59 0.08006535740746301 1104 60-61 -0.0015643542632304275 1104 62-63 -0.040508848436395795 1104 64-65 -0.032898219970029174 1104 66-67 0.07442031170398167 1104 68-69 0.03786166319444817 1104 70-71 0.051367929316121774 1104 72-73 -0.022516783760430314 1104 74-75 -0.23074644444147907 1104 76-77 -0.19970842364485009 1104 78-79 -0.23810832847295416 1104 80-81 -0.20616235422764362 1104 82-83 -0.0426129410953493 1104 84-85 -0.12332925015543594 1104 86-87 -0.23945480550416676 1104 88-89 -0.19072292118633527 1104 90-91 -0.25254813904042095 1104 92-93 -0.2063958693766068 1104 94-95 -0.23954130605312685 1104 96-97 -0.2290824510711147 1104 98-99 -0.12604965577354932 1104 100-101 -0.18993842419708074 1104 102-103 -0.18788691425803705 1104 104-105 -0.12161542991240992 1104 106-107 -0.17514589830538796 1104 108-109 -0.12495386726100577 1104 110-111 -0.09944787552775125 1104 112-113 0.00870561339804965 1104 114-115 -0.0458209431054577 1104 116-117 -0.0682477479361161 1104 118-119 -0.026355994902630187 1104 120-121 -0.09241701678886116 1104 122-123 -0.009173787749670481 1104 124-125 0.04159810575360723 1105 1 0.07957173535315576 1105 2 0.11134670530222479 1105 3 0.11250546471681133 1105 4 0.05778327439082176 1105 5 0.0070647635535223685 1105 6 0.09032977903040518 1105 7 0.0715587155788171 1105 8 -0.048457369228444236 1105 9 0.02276390511266868 1105 10-11 0.06013482673292714 1105 12-13 0.08504552197712201 1105 14-15 0.1126797818145846 1105 16-17 0.14229114640236418 1105 18-19 0.11658238913516783 1105 20-21 0.12969536126487924 1105 22-23 0.0844985540020744 1105 24-25 0.11201875311729736 1105 26-27 0.06059600068977744 1105 28-29 0.05883968167832876 1105 30-31 0.12455021617135742 1105 32-33 0.16213818384871104 1105 34-35 0.17373656829489903 1105 36-37 0.1000121109294696 1105 38-39 0.1755130843693422 1105 40-41 0.021102206481479868 1105 42-43 -0.038845508109446314 1105 44-45 -0.273053069203705 1105 46-47 -0.23936420992512097 1105 48-49 -0.07093239462795964 1105 50-51 -0.19310203500418055 1105 52-53 -0.29005672783713266 1105 54-55 -0.2423141317917441 1105 56-57 -0.3250214618129377 1105 58-59 -0.36053293319083224 1105 60-61 -0.3856669183657928 1105 62-63 -0.4361254540530055 1105 64-65 -0.4244244714962804 1105 66-67 -0.46725856497489104 1105 68-69 -0.3631578728250915 1105 70-71 -0.18767968973149607 1105 72-73 -0.1134081146517616 1105 74-75 -0.2007952844903116 1105 76-77 -0.19293186686829955 1105 78-79 -0.14049538586001376 1105 80-81 -0.20387297693827122 1105 82-83 -0.17504273352513877 1105 84-85 -0.07850019032638045 1105 86-87 -0.15839253444188728 1105 88-89 -0.14133342179682984 1105 90-91 -0.16535033684261435 1105 92-93 -0.1560295690103004 1105 94-95 -0.18418111069293275 1105 96-97 -0.29443654142519904 1105 98-99 -0.182069191793083 1105 100-101 -0.13252694678560317 1105 102-103 -0.16163538800650912 1105 104-105 -0.19885596715294795 1105 106-107 -0.20556103872052844 1105 108-109 -0.34034458765172104 1105 110-111 -0.26235385843373393 1105 112-113 -0.14731392262149257 1105 114-115 -0.1533301006146175 1105 116-117 -0.17604994573831334 1105 118-119 -0.15579433434097112 1105 120-121 -0.18681262118446185 1105 122-123 -0.0906939342698152 1105 124-125 0.054681133836638196 1106 1 0.050145605927028214 1106 2 0.04936054331606243 1106 3 0.07803253024387402 1106 4 0.09152360813115479 1106 5 0.12224587873463832 1106 6 0.09224268094330768 1106 7 0.14437108839118906 1106 8 0.10697608620500887 1106 9 0.06476634711511053 1106 10-11 0.10384667044477425 1106 12-13 0.12922541615701988 1106 14-15 0.1415768107116051 1106 16-17 0.17788333199455053 1106 18-19 0.10374152629430711 1106 20-21 0.15682193839145242 1106 22-23 0.20765687716039594 1106 24-25 0.19115998225851882 1106 26-27 0.23965582974960142 1106 28-29 0.19803382087246746 1106 30-31 0.14398448560562827 1106 32-33 0.18586630757683054 1106 34-35 0.2329144274727568 1106 36-37 0.1881683990857539 1106 38-39 0.17229778115403604 1106 40-41 0.11493615031542959 1106 42-43 0.13518786592392473 1106 44-45 0.175725929575286 1106 46-47 0.07593900537809617 1106 48-49 0.06732564363007754 1106 50-51 -0.09039548229763028 1106 52-53 -0.014965152745553212 1106 54-55 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2216 50-51 -0.9185053550883566 2216 52-53 -0.8954109480849652 2216 54-55 -0.9334995421260857 2216 56-57 -0.9436677091825914 2216 58-59 -0.965442916824216 2216 60-61 -0.8158399368366744 2216 62-63 -0.8434436677541939 2216 64-65 -0.8304801179774657 2216 66-67 -0.869269320177132 2216 68-69 -0.8350072999936629 2216 70-71 -0.7929988386676712 2216 72-73 -0.8644485635645509 2216 74-75 -0.8510875454208744 2216 76-77 -0.9011268633611706 2216 78-79 -0.836029638841076 2216 80-81 -0.8163700543289636 2216 82-83 -0.8250310430879111 2216 84-85 -0.8630337418812104 2216 86-87 -0.8088367710563418 2216 88-89 -0.8522055284136201 2216 90-91 -0.8252988989188381 2216 92-93 -0.8457069129429655 2216 94-95 -0.8920370845063559 2216 96-97 -0.8047241261808722 2216 98-99 -0.7738274335513253 2216 100-101 -0.9048556818802993 2216 102-103 -0.8961104873752248 2216 104-105 -0.9710210361265297 2216 106-107 -1.0012823386971448 2216 108-109 -1.0027130702329714 2216 110-111 -1.0091506786348106 2216 112-113 -0.9256983041973612 2216 114-115 -0.8849293524266315 2216 116-117 -0.9856945564467559 2216 118-119 -0.9622357652504903 2216 120-121 -0.9681313025031457 2216 122-123 -1.0212761180434882 2216 124-125 -1.118302265742507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 2.0 4 15.0 5 33.0 6 114.0 7 225.0 8 381.0 9 667.0 10 1358.0 11 2089.0 12 2555.0 13 4230.0 14 7854.0 15 8204.0 16 3367.0 17 2352.0 18 2175.0 19 1894.0 20 1677.0 21 1468.0 22 1537.0 23 1638.0 24 1941.0 25 2358.0 26 2829.0 27 3470.0 28 4209.0 29 4845.0 30 6140.0 31 7524.0 32 9822.0 33 12910.0 34 19043.0 35 37098.0 36 80022.0 37 268162.0 38 206907.0 39 2036.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41648667904607 18.5117501528726 13.844928276101944 22.226834891979376 2 15.447252514572812 19.93583334618723 41.751093399220075 22.865820740019885 3 19.434461503082755 25.873540476834822 29.23196865784865 25.460029362233772 4 12.132300801078403 16.711717088034376 38.06337014596337 33.092611964923854 5 12.990197453694613 38.09330093873268 33.83126939327457 15.085232214298138 6 30.527437698963638 37.33139803946317 17.451582593574262 14.689581667998935 7 27.591868899536294 33.77965486151132 20.640021986558473 17.98845425239391 8 26.140167114670838 35.27370320670029 19.399912782912182 19.186216895716687 9 27.130123127531853 16.13796565441561 20.074933780916844 36.65697743713569 10-11 24.87313559452748 27.1329275355003 26.805442794985158 21.188494074987066 12-13 26.388357219878205 24.64050995354498 27.90301796363524 21.068114862941574 14-15 23.043119174718825 25.168930524999194 27.29011460213163 24.497835698150354 16-17 21.338459791099652 28.75240302707796 27.617669452845984 22.291467728976404 18-19 21.02492698022752 26.21266106065514 31.77387217228039 20.988539786836952 20-21 22.157907799479222 25.283700921107798 32.04106214147397 20.517329137939015 22-23 21.687959134167084 25.315601061748854 31.89516281691563 21.101276987168433 24-25 21.238622867423015 25.527544193964076 32.09567798665943 21.13815495195348 26-27 21.25594008662816 25.700996826812382 31.93764959763757 21.105413488921887 28-29 21.058413837538904 25.67721336611693 32.168520033681006 21.095852762663156 30-31 21.32234939442273 25.392109091546455 32.17955234606419 21.105989167966626 32-33 21.103690325620267 25.5533104680283 32.04552401838573 21.29747518796571 34-35 21.457036289191766 25.700333725952103 31.760684839306748 21.081945145549387 36-37 21.536277303942704 25.55813798591465 31.511843539775853 21.393741170366788 38-39 21.366642267598877 25.604367713582988 31.74557100829849 21.283419010519644 40-41 21.680457342851494 25.627941999234398 31.401867455266185 21.289733202647923 42-43 21.45411178744652 25.707593138677737 31.209397089280483 21.62889798459526 44-45 21.615135453474675 25.869650569297214 30.986594873520666 21.528619103707445 46-47 21.89895143039029 26.07941814061882 30.196224700699144 21.82540572829175 48-49 21.974026374664955 26.39219290846972 29.699928415830406 21.93385230103492 50-51 22.036825145760943 26.534731628792656 29.523286695440255 21.905156530006142 52-53 22.269453126424114 26.56636701567524 29.27563544379055 21.888544414110097 54-55 21.866389591159386 26.56468437089417 29.378066444837998 22.19085959310845 56-57 22.02606602673629 26.755853077295743 29.18205904644251 22.03602184952545 58-59 22.205395385099695 26.459570368781172 29.171986401111134 22.163047845008002 60-61 22.105284560255654 26.467251767853462 29.489674726180777 21.937788945710107 62-63 21.997877060191094 26.47329779208651 29.631766695365148 21.89705845235725 64-65 22.00907524234066 26.27354171428411 29.634059642515098 22.083323400860127 66-67 22.08884024754735 26.590376726567026 29.248348714482724 22.072434311402898 68-69 22.07622757496978 26.304837384351764 29.568017567033205 22.050917473645242 70-71 21.980695465250225 26.2685120981114 29.628962270831387 22.12183016580698 72-73 22.12656854043185 26.450825407375312 29.274794121400017 22.14781193079282 74-75 21.93903913904615 26.67007869875379 29.034266383888664 22.356615778311394 76-77 22.027929126178876 26.56744630861425 29.216555547998844 22.188069017208026 78-79 21.90953611228936 26.606885575766924 29.17469142510595 22.30888688683776 80-81 21.823131598246125 26.744587142069904 29.05100236551812 22.38127889416585 82-83 21.994831476114157 26.719347470353906 29.000382801687692 22.28543825184425 84-85 22.076797579226557 26.51254521247321 28.843437066410715 22.567220141889518 86-87 21.898646938522255 26.479312861550802 29.035048912551943 22.586991287375003 88-89 21.935448137382338 26.460220174069605 28.907556897932167 22.69677479061589 90-91 21.9989834013987 26.573677107251154 29.020875326450845 22.406464164899305 92-93 21.91030733042841 26.621819163368542 28.92628907359174 22.54158443261131 94-95 21.777429403347245 26.487144773179967 28.978543889919912 22.756881933552876 96-97 21.905158530677262 26.427084582179138 28.993975392567013 22.67378149457659 98-99 21.783659556090264 26.353232010073434 28.797343664772292 23.065764769064014 100-101 21.67161412359902 26.47810242007064 28.984198518988 22.86608493734234 102-103 21.979126791490867 26.16281270901603 28.840251106689497 23.01780939280361 104-105 21.750006660468927 26.108617525025835 29.06481407476271 23.076561739742527 106-107 21.652614206329286 26.22205925450214 28.94746067157346 23.177865867595116 108-109 21.831846990857592 26.1829042571092 28.66460541813899 23.320643333894218 110-111 21.60522909909796 26.353933112065548 28.731440077513838 23.309397711322656 112-113 21.583960996211935 26.275114402558188 28.802171738098593 23.338752863131283 114-115 21.448768726069858 26.36369818977527 28.72514437837546 23.46238870577941 116-117 21.41269132371366 26.126803864228958 28.94434811994282 23.516156692114563 118-119 21.291560595405944 26.312099450263997 28.625393582510526 23.770946371819527 120-121 21.150989153213057 26.490250090968303 28.698658997518788 23.660101758299852 122-123 21.322043689994267 26.41563405738676 28.5030308724117 23.759291380207273 124-125 21.333020170930585 26.257966360749062 28.494997581170995 23.914015887149358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 270.0 2 542.0 3 819.0 4 427.0 5 234.5 6 670.0 7 1780.0 8 3141.5 9 3265.0 10 2052.5 11 1039.0 12 814.5 13 860.5 14 939.0 15 1047.0 16 1094.5 17 1115.5 18 1220.5 19 1348.5 20 1452.0 21 1582.5 22 1827.5 23 2209.0 24 2697.0 25 3329.0 26 4165.5 27 5168.5 28 6156.0 29 7242.0 30 8492.5 31 9690.0 32 11019.0 33 12326.0 34 13944.5 35 15547.0 36 17285.5 37 18834.5 38 19748.5 39 20669.0 40 21636.0 41 22708.5 42 23359.5 43 24029.0 44 25102.0 45 26435.0 46 26964.5 47 26833.5 48 26884.0 49 26374.0 50 25941.5 51 25065.0 52 23761.5 53 23285.0 54 22479.5 55 20819.5 56 18673.5 57 16530.0 58 15135.5 59 13701.0 60 11542.0 61 9227.0 62 7415.5 63 5880.0 64 4392.0 65 3334.0 66 2476.5 67 1896.0 68 1376.5 69 917.0 70 698.5 71 511.5 72 399.0 73 352.5 74 256.5 75 183.0 76 139.0 77 80.0 78 45.5 79 25.0 80 19.0 81 10.5 82 7.0 83 6.0 84 3.5 85 0.0 86 0.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021173280161758252 2 0.0028044079684448014 3 2.804407968444801E-4 4 0.1399399576253956 5 0.0402432543471829 6 0.013040497053268329 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.1033059763336012E-4 30-31 7.011019921112003E-5 32-33 1.4022039842224006E-4 34-35 4.2066119526672024E-4 36-37 0.001472314183433521 38-39 0.002944628366867042 40-41 0.0 42-43 8.413223905334405E-4 44-45 4.2066119526672024E-4 46-47 1.4022039842224006E-4 48-49 0.002313636573966961 50-51 0.0012619835858001607 52-53 0.0 54-55 0.0 56-57 0.0017527549802780012 58-59 0.002313636573966961 60-61 0.0014022039842224007 62-63 1.4022039842224006E-4 64-65 0.001963085577911361 66-67 9.114325897445606E-4 68-69 0.0012619835858001607 70-71 0.002033195777122481 72-73 0.0 74-75 7.011019921112003E-5 76-77 0.005118044542411763 78-79 7.712121913223205E-4 80-81 0.0 82-83 0.0 84-85 3.505509960556002E-4 86-87 3.505509960556002E-4 88-89 0.0 90-91 7.011019921112003E-5 92-93 7.712121913223205E-4 94-95 0.0013320937850112807 96-97 6.309917929000804E-4 98-99 0.0 100-101 2.804407968444801E-4 102-103 0.0 104-105 0.0 106-107 2.804407968444801E-4 108-109 4.2066119526672024E-4 110-111 0.0 112-113 2.1033059763336012E-4 114-115 0.0027342977692336816 116-117 9.114325897445606E-4 118-119 3.505509960556002E-4 120-121 3.505509960556002E-4 122-123 2.804407968444801E-4 124-125 0.0011217631873779205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 713163.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.13304441181061 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74363021424078 46.91571754210753 2 13.7948616791601 16.866437833737574 3 4.411285626859687 8.090259604199852 4 1.9059263675752482 4.660603250984741 5 0.9521393932644815 2.9103589907335974 6 0.5120242956645745 1.878096240407309 7 0.3549581909752142 1.5189772397256602 8 0.24753479632943945 1.2106044557980893 9 0.1652367849569246 0.9091284941912824 >10 0.8509316022904596 8.970129157769295 >50 0.04128673848157133 1.6733968517991016 >100 0.018578740072646236 1.8581838923805665 >500 2.2936716127044596E-4 0.1111937689524978 >1k 0.00114683580635223 1.544373482021199 >5k 2.2936716127044596E-4 0.8825391951917039 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6294 0.8825471876695791 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4579 0.6420692043754372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3117 0.43706698188212234 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1236 0.17331241244988874 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.14877384272599672 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1021 0.1431650267891071 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 793 0.11119477594883638 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.011019921112004E-5 0.0 16-17 0.0 0.0 0.0 2.804407968444802E-4 0.0 18-19 0.0 0.0 0.0 4.907713944778402E-4 0.0 20-21 0.0 0.0 0.0 5.608815936889604E-4 0.0 22-23 0.0 0.0 0.0 7.011019921112005E-4 0.0 24-25 0.0 0.0 0.0 0.0010516529881668007 0.0 26-27 0.0 0.0 0.0 0.0014022039842224007 0.0 28-29 0.0 0.0 0.0 0.0023837467731780814 0.0 30-31 0.0 0.0 0.0 0.006730579124267524 0.0 32-33 0.0 0.0 0.0 0.013741599045379527 0.0 34-35 0.0 0.0 0.0 0.021243390360969373 0.0 36-37 0.0 0.0 0.0 0.03596653219530458 0.0 38-39 0.0 0.0 0.0 0.05854201634128523 0.0 40-41 0.0 0.0 0.0 0.08721708781863333 0.0 42-43 0.0 0.0 0.0 0.1137187431204367 0.0 44-45 0.0 0.0 0.0 0.1419731534025181 0.0 46-47 0.0 0.0 0.0 0.17927177938283395 0.0 48-49 0.0 0.0 0.0 0.2235814252842618 0.0 50-51 0.0 0.0 0.0 0.2691530547714899 0.0 52-53 0.0 0.0 0.0 0.3136029210713399 0.0 54-55 0.0 0.0 0.0 0.360366423945157 0.0 56-57 0.0 0.0 0.0 0.4153328201266751 0.0 58-59 0.0 0.0 0.0 0.4867050029235953 0.0 60-61 0.0 0.0 0.0 0.5676121728132277 0.0 62-63 0.0 0.0 0.0 0.6435415185588709 0.0 64-65 0.0 0.0 0.0 0.7225557130698032 0.0 66-67 0.0 0.0 0.0 0.8072488337168361 0.0 68-69 0.0 0.0 0.0 0.9075765287879489 0.0 70-71 0.0 0.0 0.0 1.0334944465711207 0.0 72-73 0.0 0.0 0.0 1.1562574053897916 0.0 74-75 0.0 0.0 0.0 1.2784594826147737 0.0 76-77 0.0 0.0 0.0 1.4176983382480584 0.0 78-79 0.0 0.0 0.0 1.5777599230470454 0.0 80-81 0.0 0.0 0.0 1.7595556696014796 0.0 82-83 0.0 0.0 0.0 1.952428827631271 0.0 84-85 0.0 0.0 0.0 2.1379404147438947 0.0 86-87 0.0 0.0 0.0 2.326466740422596 0.0 88-89 0.0 0.0 0.0 2.5525020226792474 0.0 90-91 0.0 0.0 0.0 2.80244488286689 0.0 92-93 0.0 0.0 0.0 3.073420802817869 0.0 94-95 0.0 0.0 0.0 3.336965041652469 0.0 96-97 0.0 0.0 0.0 3.6080110718026592 0.0 98-99 0.0 0.0 0.0 3.90724140203572 0.0 100-101 0.0 0.0 0.0 4.26781815657851 0.0 102-103 0.0 0.0 0.0 4.653634582837304 0.0 104-105 0.0 0.0 0.0 5.030476903597074 0.0 106-107 0.0 0.0 0.0 5.393016743717776 0.0 108-109 0.0 0.0 0.0 5.782268569737914 0.0 110-111 0.0 0.0 0.0 6.230623293693027 0.0 112-113 0.0 0.0 0.0 6.710808048089988 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2015 0.0 74.312096 1 GTATCAA 4170 0.0 64.23491 1 TAACGCA 120 0.0 54.673973 4 TATCACG 70 8.9848254E-7 51.12372 2 ATCAACG 5320 0.0 50.226807 3 TCAACGC 5395 0.0 49.418015 4 TATCAAC 5415 0.0 49.34564 2 ATAACGC 135 0.0 48.59909 3 CAACGCA 5495 0.0 48.53172 5 GTGGTAT 805 0.0 47.4291 1 AACGCAG 5635 0.0 47.30273 6 ACGCAGA 6410 0.0 41.58069 7 TGGTATC 875 0.0 41.580624 2 TATAACG 175 0.0 40.898975 2 CGCAGAG 6540 0.0 40.481255 8 GCAGAGT 6935 0.0 38.003967 9 ATCACGC 115 6.5277527E-7 36.30525 3 ACTGTCG 120 9.2894516E-7 34.704712 8 TACATGG 4015 0.0 34.01881 2 GTACATG 4065 0.0 33.754223 1 >>END_MODULE