FastQCFastQC Report
Fri 27 May 2016
SRR940007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281557
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79282.815770874103645No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16750.5949061824071148No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13500.47947662462662977No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8120.2883963105161655No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7930.2816481209843833No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5420.1925009855908395No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4300.15272218414033392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.14632916247864552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.12111224370198574No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.11116754333935935No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.10548485741785854No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGGC250.002034718271.580732
ACTGCGC300.004208826559.586928
GTACAAG1750.051.13821
ATCGCGT255.331623E-447.5975524-25
GTATCAA10300.047.4972951
CGCGTCA300.001311134439.64701526-27
GGTATCA6050.038.459311
GAGAACA5900.037.4146731
GTACATG23450.036.636321
GAACAAA5250.035.2285351
GTAGCAC851.6873097E-435.0698553
CATCGCG350.002770316334.0436122-23
GCGTCAA350.002794565633.98315426-27
CGTCAAC350.002794565633.98315428-29
TACATGG23400.033.9039762
ATCAACG12350.033.7920043
TCAACGC12350.033.7920044
GAAAACA6450.033.2992941
CAACGCA12700.032.8431855
AACGCAG12900.032.333996
Produced by FastQC (version 0.11.5)