##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 281557 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.761899011567817 33.0 30.0 34.0 12.0 34.0 2 28.88815053435006 33.0 30.0 34.0 12.0 34.0 3 29.40912497291845 34.0 30.0 34.0 15.0 34.0 4 31.684380072241144 37.0 35.0 37.0 12.0 37.0 5 32.638506590139826 37.0 35.0 37.0 17.0 37.0 6 32.05783553596608 37.0 35.0 37.0 12.0 37.0 7 32.32536573411423 37.0 35.0 37.0 13.0 37.0 8 31.942839282987105 37.0 35.0 37.0 8.0 37.0 9 33.073125512773615 39.0 35.0 39.0 8.0 39.0 10-11 33.445581178944224 39.0 35.0 39.0 10.0 39.0 12-13 33.35317537834257 39.0 35.0 39.0 8.0 39.0 14-15 34.63094506618553 41.0 36.0 41.0 8.0 41.0 16-17 34.76998973564855 41.0 36.0 41.0 8.0 41.0 18-19 34.723823240054415 41.0 35.5 41.0 10.0 41.0 20-21 34.73734270502953 41.0 35.0 41.0 12.5 41.0 22-23 34.83850516946835 41.0 35.0 41.0 15.0 41.0 24-25 35.02012558735886 41.0 35.0 41.0 19.5 41.0 26-27 35.11334117070433 41.0 35.0 41.0 23.5 41.0 28-29 35.070989178035 41.0 35.0 41.0 24.5 41.0 30-31 34.99682302340201 41.0 35.0 41.0 24.5 41.0 32-33 34.92613041053855 40.0 34.5 41.0 24.0 41.0 34-35 34.80587944892153 40.0 34.0 41.0 23.5 41.0 36-37 34.619506884929166 40.0 34.0 41.0 21.0 41.0 38-39 34.48343141886012 40.0 34.0 41.0 17.0 41.0 40-41 34.37943116313926 40.0 33.0 41.0 14.5 41.0 42-43 34.17915910455076 40.0 33.0 41.0 8.0 41.0 44-45 34.05213686749042 40.0 33.0 41.0 3.5 41.0 46-47 33.913685328370455 40.0 33.0 41.0 2.0 41.0 48-49 33.77276537255334 40.0 33.0 41.0 2.0 41.0 50-51 33.63072663794543 40.0 32.0 41.0 2.0 41.0 52-53 33.730077746246764 40.0 33.0 41.0 2.0 41.0 54-55 33.55369250276143 40.0 32.0 41.0 2.0 41.0 56-57 33.46065450335101 39.0 32.0 41.0 2.0 41.0 58-59 33.26333921728104 39.0 32.0 41.0 2.0 41.0 60-61 33.02412832925482 39.0 32.0 41.0 2.0 41.0 62-63 32.719399624232395 38.0 31.0 41.0 2.0 41.0 64-65 32.40539393444311 37.0 31.0 41.0 2.0 41.0 66-67 32.07865192483227 37.0 31.0 40.0 2.0 41.0 68-69 31.721402771019726 36.0 31.0 40.0 2.0 41.0 70-71 31.36334028278466 36.0 31.0 39.0 2.0 41.0 72-73 30.990632447426275 35.0 30.0 39.0 2.0 41.0 74-75 30.62642555503859 35.0 30.0 38.5 2.0 40.5 76-77 30.27588729813146 35.0 30.0 37.0 2.0 39.5 78-79 29.95215000870161 35.0 30.0 37.0 2.0 39.0 80-81 29.64669676122419 35.0 30.0 36.0 2.0 39.0 82-83 29.352358492241358 35.0 29.5 36.0 2.0 37.5 84-85 29.10737967800481 35.0 29.0 35.5 2.0 37.0 86-87 28.896274999378456 35.0 29.0 35.0 2.0 37.0 88-89 28.713155773076146 35.0 29.0 35.0 2.0 36.0 90-91 28.55505989906129 35.0 29.0 35.0 2.0 36.0 92-93 28.402213406166425 35.0 28.5 35.0 2.0 36.0 94-95 28.266848275837575 35.0 28.0 35.0 2.0 36.0 96-97 28.16071523705679 35.0 28.0 35.0 2.0 35.0 98-99 28.07499191993096 35.0 28.0 35.0 2.0 35.0 100-101 27.97584680899427 35.0 28.0 35.0 2.0 35.0 102-103 27.369072692207972 33.5 27.0 34.5 2.0 35.0 104-105 28.090695667307152 35.0 28.0 35.0 2.0 35.0 106-107 28.162389853564285 35.0 28.0 35.0 2.0 35.0 108-109 28.129815632358635 35.0 28.0 35.0 2.0 35.0 110-111 28.068870601689888 35.0 28.0 35.0 2.0 35.0 112-113 27.995434317029947 35.0 28.0 35.0 2.0 35.0 114-115 27.91127373853252 35.0 28.0 35.0 2.0 35.0 116-117 27.828567572463122 35.0 28.0 35.0 2.0 35.0 118-119 27.730622928927357 35.0 27.0 35.0 2.0 35.0 120-121 27.61123857691338 35.0 27.0 35.0 2.0 35.0 122-123 27.46402682227755 35.0 27.0 35.0 2.0 35.0 124-125 26.406139786970314 33.5 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0839569262330357 1101 2 0.9925010859296322 1101 3 1.697064417734552 1101 4 1.4424089785004135 1101 5 1.563167762524003 1101 6 1.2373449862351649 1101 7 1.02976955598416 1101 8 1.3360118851822733 1101 9 1.3604507489440536 1101 10-11 1.365600818633716 1101 12-13 1.3221299520183223 1101 14-15 1.3426103203637965 1101 16-17 1.3732615426230836 1101 18-19 1.3449388282747492 1101 20-21 1.3129530118637192 1101 22-23 1.6667323892893364 1101 24-25 1.1810593532197444 1101 26-27 0.9848052397243947 1101 28-29 1.170556583866201 1101 30-31 1.286696403278519 1101 32-33 1.191160211106073 1101 34-35 1.2049519303967955 1101 36-37 1.2133543385988972 1101 38-39 1.1689709607593528 1101 40-41 1.090023809714694 1101 42-43 1.2935270215082681 1101 44-45 1.3216658989903465 1101 46-47 1.3266403847941106 1101 48-49 1.2714420326336366 1101 50-51 1.273024841798005 1101 52-53 1.2393346804442658 1101 54-55 1.3019184777782513 1101 56-57 1.3106337885730497 1101 58-59 1.3017881679526297 1101 60-61 1.2409066208135542 1101 62-63 1.3297306487606235 1101 64-65 1.3542215275629275 1101 66-67 1.171167645061871 1101 68-69 1.0482566072876836 1101 70-71 1.1001874948810055 1101 72-73 0.9578816755602269 1101 74-75 1.0220102067749792 1101 76-77 0.8285022237392212 1101 78-79 0.7588396747187396 1101 80-81 0.7114474740224637 1101 82-83 0.6891441048891807 1101 84-85 0.7575888762929139 1101 86-87 0.7184634982194886 1101 88-89 0.7462238774546677 1101 90-91 0.7825509555552053 1101 92-93 0.7858635995721599 1101 94-95 0.6788085200093299 1101 96-97 0.6799200268294179 1101 98-99 0.6160871210169176 1101 100-101 0.6557744196815989 1101 102-103 0.36288548310514557 1101 104-105 0.7514936110860297 1101 106-107 0.7999390831359108 1101 108-109 0.9048649557732098 1101 110-111 1.0345844544052518 1101 112-113 0.953355443812189 1101 114-115 0.9375971604774591 1101 116-117 1.0244186100729706 1101 118-119 1.0300095447050595 1101 120-121 0.7502085214597436 1101 122-123 0.778800651874004 1101 124-125 0.7634774644125102 1102 1 0.5171314849329498 1102 2 0.18562240842893019 1102 3 0.5631683684210174 1102 4 0.29271718808292846 1102 5 0.5437645683519676 1102 6 0.5435582112281594 1102 7 0.530960365734785 1102 8 0.179910736401105 1102 9 0.5332932818664418 1102 10-11 0.5653941781181686 1102 12-13 0.4216326138283577 1102 14-15 0.48932508640470473 1102 16-17 0.5256151430713913 1102 18-19 0.47267068593796324 1102 20-21 0.33011468179927306 1102 22-23 0.14056959859166795 1102 24-25 0.22478658773922433 1102 26-27 0.45787192507042107 1102 28-29 0.46670046170313384 1102 30-31 0.5083271121605648 1102 32-33 0.5579996619326337 1102 34-35 0.7092423478806893 1102 36-37 0.5076419541774868 1102 38-39 0.7842343398571359 1102 40-41 0.751728769087066 1102 42-43 0.8181837881095575 1102 44-45 0.7563639936947482 1102 46-47 0.671117128980157 1102 48-49 0.8174633271138916 1102 50-51 0.9045567835183732 1102 52-53 0.9579358292254341 1102 54-55 1.035667427063764 1102 56-57 0.9120452483657076 1102 58-59 0.9630903534387656 1102 60-61 0.842002866260458 1102 62-63 0.9397404554291526 1102 64-65 1.0096222000201962 1102 66-67 0.8120334337982129 1102 68-69 0.8818059208208879 1102 70-71 0.8573498652928819 1102 72-73 0.698968114338598 1102 74-75 0.7064281109498189 1102 76-77 0.7006477827193294 1102 78-79 0.735076015430419 1102 80-81 0.749973671836976 1102 82-83 0.8140215215324993 1102 84-85 0.6931800783102844 1102 86-87 0.601676583117218 1102 88-89 0.6897654857483104 1102 90-91 0.5501153993770025 1102 92-93 0.5838112039431351 1102 94-95 0.586906026313617 1102 96-97 0.5642363619372865 1102 98-99 0.7031738399858156 1102 100-101 0.6518867760839484 1102 102-103 0.6355809159995118 1102 104-105 0.7733659842998115 1102 106-107 0.9412076457584959 1102 108-109 0.5812974298555886 1102 110-111 0.5790296771567256 1102 112-113 0.703845777238918 1102 114-115 0.7338636216710697 1102 116-117 0.707995942659128 1102 118-119 0.5533178943828432 1102 120-121 0.5601692947856058 1102 122-123 0.45691426913374045 1102 124-125 0.4540420483295762 1103 1 0.19430583632426845 1103 2 0.44772080875155496 1103 3 0.3346262256598962 1103 4 0.8501845896153917 1103 5 0.1726461384554696 1103 6 0.34012024913648276 1103 7 0.7050429037103214 1103 8 0.3230583368850404 1103 9 0.27769777171722865 1103 10-11 0.3018254860896761 1103 12-13 0.5611002842141559 1103 14-15 0.6585010509160654 1103 16-17 0.7342285319677444 1103 18-19 0.7700728905353529 1103 20-21 0.48796519934195715 1103 22-23 0.6139867245494486 1103 24-25 0.4986389200419481 1103 26-27 0.5853084084687339 1103 28-29 0.5465751088331174 1103 30-31 0.35133356464073273 1103 32-33 0.4155578014675214 1103 34-35 0.47991885009872703 1103 36-37 0.41356849000219853 1103 38-39 0.50859245250669 1103 40-41 0.4770110650946009 1103 42-43 0.22193429220956773 1103 44-45 0.22352187781895339 1103 46-47 0.22042651196174035 1103 48-49 0.1441873491598571 1103 50-51 0.2331543772809752 1103 52-53 0.23350210157386897 1103 54-55 0.35219182550441275 1103 56-57 0.22835960513408793 1103 58-59 0.26875139658972813 1103 60-61 0.4111569864379021 1103 62-63 0.45516591584050303 1103 64-65 0.7013818451337883 1103 66-67 0.7191950146558526 1103 68-69 0.6620267166267695 1103 70-71 0.8146609958211783 1103 72-73 0.737523363700074 1103 74-75 0.9623176794402042 1103 76-77 0.9080429299165331 1103 78-79 0.617595507297608 1103 80-81 0.5649017535683889 1103 82-83 0.7241656941363459 1103 84-85 0.5203073803670151 1103 86-87 0.616959215191418 1103 88-89 0.7098790762846718 1103 90-91 0.6644368807589096 1103 92-93 0.5110359653357932 1103 94-95 0.6868522556455154 1103 96-97 0.92362606485435 1103 98-99 0.6677272073739076 1103 100-101 0.6216407752773918 1103 102-103 0.6681121702003452 1103 104-105 0.7359197549679131 1103 106-107 0.8272390343859968 1103 108-109 0.6848983842849456 1103 110-111 0.7263108977508885 1103 112-113 0.7439049249738332 1103 114-115 0.6846634603590687 1103 116-117 0.46013836771625805 1103 118-119 0.5510242393216771 1103 120-121 0.7130628288627641 1103 122-123 0.8266800306608282 1103 124-125 0.6610591205166969 1104 1 -0.0420803641388261 1104 2 -0.12697393229890253 1104 3 -0.06540042723610995 1104 4 0.4852981142943058 1104 5 -0.04300910633472199 1104 6 0.1110386079844119 1104 7 0.4483921732543905 1104 8 0.6504974933208771 1104 9 0.37146599147035886 1104 10-11 0.426223713672087 1104 12-13 0.44002950411721287 1104 14-15 0.5752107509044038 1104 16-17 0.35780635009504635 1104 18-19 0.09544228301590607 1104 20-21 0.02586228584726058 1104 22-23 0.15556524231527646 1104 24-25 0.413199730289989 1104 26-27 0.1981875875595307 1104 28-29 0.2417867494854491 1104 30-31 0.11357307422744611 1104 32-33 0.365125524810459 1104 34-35 0.295643225837928 1104 36-37 0.32226982764441914 1104 38-39 0.23579589739349416 1104 40-41 0.13262649712138597 1104 42-43 0.27653108386721215 1104 44-45 0.2684819817652482 1104 46-47 0.2686432666384917 1104 48-49 0.2434568932287604 1104 50-51 0.17413070617771353 1104 52-53 -0.14431519282402405 1104 54-55 0.01917221896778898 1104 56-57 0.10489006439679827 1104 58-59 0.20772459377118224 1104 60-61 0.11978669442211043 1104 62-63 0.26828746970434025 1104 64-65 0.2798621303197777 1104 66-67 0.3100647319685734 1104 68-69 0.22815921483602608 1104 70-71 0.1666999423654687 1104 72-73 0.4079311530089278 1104 74-75 0.461634698980113 1104 76-77 0.60738576944944 1104 78-79 0.44841208539892463 1104 80-81 0.6720130793833867 1104 82-83 0.5636319698938692 1104 84-85 0.6925433917611272 1104 86-87 0.531594986709468 1104 88-89 0.6333935937839641 1104 90-91 0.4963195747003759 1104 92-93 0.3712165387062214 1104 94-95 0.34230110707227723 1104 96-97 0.38274718560862553 1104 98-99 0.31226086498413963 1104 100-101 0.34440068265857704 1104 102-103 0.24979913193678271 1104 104-105 0.19574467875728985 1104 106-107 0.4753583394117342 1104 108-109 0.42285044238825975 1104 110-111 0.3879315604085143 1104 112-113 0.48206104431217156 1104 114-115 0.17540574351277272 1104 116-117 0.11609945448809711 1104 118-119 -0.0034267943109469456 1104 120-121 -0.08218879113357147 1104 122-123 -0.02173578783295227 1104 124-125 0.06410504678812501 1105 1 -0.5746435774767207 1105 2 -0.7933882340621992 1105 3 -0.4040008603268461 1105 4 -0.8190946631849734 1105 5 -0.5495543122156334 1105 6 -0.5353900215927574 1105 7 -0.960105197266131 1105 8 -0.7730685041717535 1105 9 -0.6875548327045706 1105 10-11 -0.6605206162583883 1105 12-13 -0.7145847032127719 1105 14-15 -0.7841935051075737 1105 16-17 -0.7486753575198648 1105 18-19 -0.925617975210379 1105 20-21 -0.8942316590232195 1105 22-23 -0.846685689806705 1105 24-25 -0.9439576640016796 1105 26-27 -1.0111531701864394 1105 28-29 -0.904251121531658 1105 30-31 -0.7348899421030026 1105 32-33 -0.789244458419482 1105 34-35 -0.6501634463644876 1105 36-37 -0.7176323198995007 1105 38-39 -0.8707387254430898 1105 40-41 -0.8351684848874328 1105 42-43 -0.7793066862039382 1105 44-45 -0.9465084934948464 1105 46-47 -0.895817242324533 1105 48-49 -0.703066169667089 1105 50-51 -0.8229182526135403 1105 52-53 -0.7024323878288214 1105 54-55 -0.4782105127601781 1105 56-57 -0.5972094487831612 1105 58-59 -0.5728034187330948 1105 60-61 -0.49518180066898054 1105 62-63 -0.38176788775833614 1105 64-65 -0.567371624707171 1105 66-67 -0.6331938687901442 1105 68-69 -0.8306743708622051 1105 70-71 -0.750988395119677 1105 72-73 -0.7001827856745386 1105 74-75 -0.7252415119643842 1105 76-77 -0.795697966853659 1105 78-79 -0.7359445469135544 1105 80-81 -0.8578636688541295 1105 82-83 -0.8623925502057759 1105 84-85 -0.7194463833898865 1105 86-87 -0.878721853356307 1105 88-89 -0.9096793966587349 1105 90-91 -1.0352626718357136 1105 92-93 -0.8269265440531868 1105 94-95 -0.7848256777988531 1105 96-97 -0.8051303441460114 1105 98-99 -1.0406755227786029 1105 100-101 -0.9860385950893473 1105 102-103 -1.0317098473692994 1105 104-105 -0.927995531926701 1105 106-107 -0.8134977149037041 1105 108-109 -0.651104685503757 1105 110-111 -0.6334095555328041 1105 112-113 -0.5777860714012064 1105 114-115 -0.7947134378480456 1105 116-117 -0.6741893812142123 1105 118-119 -0.6852923408030733 1105 120-121 -0.5641080669277052 1105 122-123 -0.6230440149288015 1105 124-125 -0.7743773446542619 1106 1 0.2881852838050136 1106 2 0.5954031574308942 1106 3 0.3509907497656748 1106 4 0.45256130442536247 1106 5 0.7544451335886393 1106 6 0.18121583748758496 1106 7 0.2003729504886067 1106 8 0.44853281358252417 1106 9 0.15859427673368742 1106 10-11 0.47346004846858847 1106 12-13 0.5291036232700961 1106 14-15 0.37393315940388305 1106 16-17 0.5544235202337973 1106 18-19 0.628292953221397 1106 20-21 0.39983459815438493 1106 22-23 0.6112146781176762 1106 24-25 0.6741283594900622 1106 26-27 0.7135116183724932 1106 28-29 0.7128073307228462 1106 30-31 0.6958548353720104 1106 32-33 0.6689637960179269 1106 34-35 0.8387295476779144 1106 36-37 0.9318427335957793 1106 38-39 0.9108581279567503 1106 40-41 0.9060836895610578 1106 42-43 0.7964487903274176 1106 44-45 1.0249504267471536 1106 46-47 0.92984201967149 1106 48-49 0.8182395770346815 1106 50-51 0.7873628477728545 1106 52-53 0.7589663405998692 1106 54-55 0.9878488477595297 1106 56-57 1.1037750183422617 1106 58-59 0.999807766783924 1106 60-61 1.0917691991924627 1106 62-63 1.1819145888807796 1106 64-65 1.138400477695889 1106 66-67 1.1678806551403298 1106 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0.28423427883784314 2204 54-55 0.5077216906296513 2204 56-57 0.5497623026292757 2204 58-59 0.4831174852217046 2204 60-61 0.47499899029146775 2204 62-63 0.6043451065919427 2204 64-65 0.7713741380046955 2204 66-67 0.7469869221703007 2204 68-69 0.6380035952394962 2204 70-71 0.6735087800215709 2204 72-73 0.7543595872068174 2204 74-75 0.8653234597870281 2204 76-77 0.8240082478356072 2204 78-79 0.6229481084536772 2204 80-81 0.6551984780385247 2204 82-83 0.8002967153309477 2204 84-85 0.6702033341242384 2204 86-87 0.6929052652877594 2204 88-89 0.41208331520567043 2204 90-91 0.6517316784467724 2204 92-93 0.599028258206701 2204 94-95 0.5689370340655522 2204 96-97 0.4813216332551171 2204 98-99 0.5689063981253533 2204 100-101 0.5819953032157343 2204 102-103 0.6281545594103619 2204 104-105 0.5618349765478747 2204 106-107 0.5393045449833025 2204 108-109 0.4635958794679915 2204 110-111 0.3953475066140868 2204 112-113 0.3307450020451199 2204 114-115 0.257569048027662 2204 116-117 0.20263547754285227 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0.11378260971176957 2205 52-53 0.24800620866240308 2205 54-55 0.23254625203316692 2205 56-57 0.40942896929593786 2205 58-59 0.17666134487082985 2205 60-61 0.20961302537918414 2205 62-63 0.2886082644866903 2205 64-65 0.24456712046083595 2205 66-67 0.22861850111767268 2205 68-69 0.20605622681843982 2205 70-71 0.26428070984612617 2205 72-73 0.22560520124190475 2205 74-75 0.32400767031334254 2205 76-77 0.35500824783560603 2205 78-79 0.27410600319052136 2205 80-81 0.2312160218981738 2205 82-83 0.3215949609449851 2205 84-85 0.3296243867558175 2205 86-87 0.17320351090179642 2205 88-89 0.008890332749530216 2205 90-91 0.22246852055203448 2205 92-93 0.1752738722417888 2205 94-95 0.299972121784851 2205 96-97 0.26233917711476806 2205 98-99 0.2524853454937741 2205 100-101 0.21143389970696091 2205 102-103 0.321382629585802 2205 104-105 0.33990515198647486 2205 106-107 0.44832208884295 2205 108-109 0.3161046513978185 2205 110-111 0.3902597873158413 2205 112-113 0.3524993880100311 2205 114-115 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48-49 0.5354365857848151 2206 50-51 0.5086972612053628 2206 52-53 0.5584193730229288 2206 54-55 0.5683441312520969 2206 56-57 0.5134215982885735 2206 58-59 0.09982182592604971 2206 60-61 0.23195558324805887 2206 62-63 0.17714117538801588 2206 64-65 0.32101869164925034 2206 66-67 0.3418960130793991 2206 68-69 0.37167435691024764 2206 70-71 0.21787569238848192 2206 72-73 0.05207293692016535 2206 74-75 0.21918095364452128 2206 76-77 0.3244799923073529 2206 78-79 0.2905549880605598 2206 80-81 0.14162272046277025 2206 82-83 0.26712063015486365 2206 84-85 0.26409031201121635 2206 86-87 0.2670707034531965 2206 88-89 0.4507434298657884 2206 90-91 0.3117611624762553 2206 92-93 0.22219615337459686 2206 94-95 0.4273580004865174 2206 96-97 0.4475001754336638 2206 98-99 0.5113240885430415 2206 100-101 0.5016382188586519 2206 102-103 0.5024313816871881 2206 104-105 0.44504545975835796 2206 106-107 0.45805311173187135 2206 108-109 0.39062618249829484 2206 110-111 0.4376734689400479 2206 112-113 0.34230929360941076 2206 114-115 0.4120391544977693 2206 116-117 0.428758327665701 2206 118-119 0.2744516867375637 2206 120-121 0.3651732945570423 2206 122-123 0.38093646786524005 2206 124-125 0.2403542590603891 2207 1 0.32363408145586803 2207 2 0.2835000331749242 2207 3 0.2438242726883466 2207 4 -0.15134467689788167 2207 5 0.2553247399482643 2207 6 0.33908203150361516 2207 7 0.3608316685119348 2207 8 0.3643485327433922 2207 9 -0.017254245792173606 2207 10-11 0.4085444377704661 2207 12-13 0.15148133814535925 2207 14-15 0.46793199541351527 2207 16-17 0.42519744155862327 2207 18-19 0.19752392594698875 2207 20-21 0.461069750663583 2207 22-23 0.5526547193864246 2207 24-25 0.32332745475209634 2207 26-27 0.29321850443371034 2207 28-29 0.306859075385681 2207 30-31 0.4467443621041127 2207 32-33 0.4434704228267421 2207 34-35 0.3785102413103516 2207 36-37 0.3457967295218154 2207 38-39 0.4525567602680667 2207 40-41 0.4282358813020153 2207 42-43 0.25682682265460244 2207 44-45 0.6195808598044792 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2216 68-69 -0.7672970182574446 2216 70-71 -0.7755239398148319 2216 72-73 -0.6624154639179274 2216 74-75 -0.488633595427693 2216 76-77 -0.6914641448024312 2216 78-79 -0.747794222834667 2216 80-81 -0.7421614401618832 2216 82-83 -0.8023556364072917 2216 84-85 -0.6953917579003033 2216 86-87 -0.6969127306222624 2216 88-89 -0.607423842258541 2216 90-91 -0.7420535976268496 2216 92-93 -0.8705688788076174 2216 94-95 -0.8570588759548947 2216 96-97 -0.8265290824913016 2216 98-99 -0.8393696754942823 2216 100-101 -0.9556713634509322 2216 102-103 -0.7135059722869777 2216 104-105 -0.9164881318365836 2216 106-107 -0.9417731646281489 2216 108-109 -0.8523325458898832 2216 110-111 -0.8707670128133174 2216 112-113 -0.898897124744245 2216 114-115 -0.9273041626062835 2216 116-117 -0.862315442702549 2216 118-119 -0.9148034496034612 2216 120-121 -1.0383290644667866 2216 122-123 -1.466247023490027 2216 124-125 -1.426789739371948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13056.0 3 3268.0 4 3637.0 5 2076.0 6 1904.0 7 1685.0 8 1529.0 9 1311.0 10 1641.0 11 2007.0 12 2134.0 13 1874.0 14 1863.0 15 1734.0 16 1281.0 17 1130.0 18 882.0 19 846.0 20 669.0 21 591.0 22 705.0 23 1636.0 24 4907.0 25 4188.0 26 5742.0 27 3171.0 28 2302.0 29 2787.0 30 2526.0 31 3011.0 32 3877.0 33 5421.0 34 8044.0 35 16304.0 36 31738.0 37 83772.0 38 55923.0 39 385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49531674175988 23.98517560441747 12.272055374731726 20.247452279090922 2 13.51107030112785 28.069913195742004 37.41195898596683 21.00705751716333 3 17.06764246127611 36.2416512718488 24.517194827341196 22.17351143953389 4 10.603646914914801 24.04524507154439 33.56790316615936 31.783204847381448 5 11.535045649934277 47.14199438701197 28.297275214039573 13.025684749014173 6 25.90001421262081 43.401435474701536 16.51968447981808 14.17886583285958 7 24.790631073653095 42.10793736520265 17.700335061345008 15.401096499799246 8 22.657152710375403 43.60989934973744 17.309285351138225 16.42366258874893 9 23.904833031046625 27.633628507414915 16.922010028806145 31.539528432732318 10-11 21.12481366520323 37.81206081399212 22.69828776311184 18.364837757692808 12-13 21.837416283242437 35.592368229379474 23.389529409674726 19.180686077703363 14-15 20.287973458274575 35.56065487586984 21.99407500026641 22.15729666558918 16-17 19.48502047305639 38.2655191096663 22.616465488910798 19.632994928366507 18-19 19.600192601200025 36.43645137619243 24.990716408947073 18.972639613660473 20-21 21.77104240509032 34.967473772615335 24.39360293803647 18.867880884257875 22-23 20.965227146257906 35.300873454831205 24.120189375958926 19.61371002295197 24-25 20.320322455094608 35.14198936168322 24.89021349498741 19.647474688234762 26-27 20.061525564013937 35.35872742967365 24.802759414441454 19.776987591870952 28-29 19.907508537878343 35.136632692748265 24.987612750608708 19.968246018764685 30-31 20.11291849373336 35.08518615212206 25.008125229770844 19.793770124373737 32-33 19.814231039045225 35.109978421674235 24.9585749425022 20.117215596778347 34-35 20.144251603782298 35.015735892754385 24.985613912944636 19.85439859051868 36-37 20.23609308041621 34.741237082277394 24.86578355233598 20.156886284970422 38-39 19.973643642150282 35.238841172751364 24.883492110852995 19.904023074245362 40-41 20.031363940703326 34.96013795871539 25.207302150543363 19.80119595003792 42-43 19.989982985283515 34.922971998536525 25.04715456395793 20.039890452222036 44-45 19.927217055860645 35.092639296396015 25.043513473192146 19.93663017455119 46-47 19.666997632322616 35.32226522604836 24.884236028838416 20.12650111279061 48-49 19.703724755324252 35.267766745412885 24.91198777953427 20.11652071972859 50-51 19.930519540491193 35.223502582428125 24.833936018300783 20.0120418587799 52-53 20.04518505625042 35.19336859567545 24.77807103858136 19.983375309492768 54-55 19.83734119097224 35.27927121464817 24.795255577520475 20.088132016859113 56-57 19.923339662488477 35.613131723987266 24.447649479665294 20.015879133858967 58-59 19.747707359009016 35.28473508739833 24.819492821453757 20.14806473213889 60-61 19.71753690189274 35.75864892273647 24.770401050001155 19.75341312536964 62-63 19.72138444541544 35.7331642385941 24.769714990106536 19.775736325883926 64-65 19.5872186288112 35.716354036947095 24.625297337243943 20.07112999699776 66-67 19.711732323887244 36.06987489097592 24.263476798075846 19.954915987060996 68-69 20.0 35.933699890743384 24.37932474084865 19.686975368407964 70-71 19.652687356448432 36.035107851305 24.55702183671403 19.75518295553254 72-73 19.69805154437754 36.133461217385126 24.449832152182022 19.71865508605531 74-75 19.708333851353633 36.41120273656803 24.213511982253515 19.666951429824824 76-77 19.718597534442676 36.31111276878784 24.4641115947975 19.506178101971987 78-79 19.968772148593413 36.25219598585332 24.14811735845772 19.630914507095543 80-81 19.715055081298647 36.552063461613606 24.103796542009924 19.62908491507782 82-83 20.075100892400386 36.319487864491556 23.971181720002274 19.63422952310578 84-85 19.99737122090803 36.14482441354247 24.133435405746724 19.72436895980277 86-87 20.010267597027013 36.04477240752057 24.28641977063112 19.658540224821294 88-89 20.097595983891512 35.94139977581702 24.289391455110998 19.671612785180475 90-91 20.04846021572612 36.17427203092359 24.17024315375105 19.607024599599242 92-93 20.16787912702854 36.01851111644061 24.142262015793072 19.67134774073778 94-95 19.823393362773693 35.97094570349554 24.264494285419914 19.94116664831085 96-97 19.952570012523648 35.957970280760655 24.278824375816036 19.810635330899657 98-99 19.733420026007803 36.260987585893254 24.105892970076816 19.89969941802213 100-101 19.307853129723807 36.54740102988953 24.25693594541834 19.88780989496832 102-103 19.63306142170935 36.528294915157886 24.19403547167666 19.6446081914561 104-105 19.17171893013399 37.01294433421145 24.16417828257977 19.651158453074792 106-107 19.001794247748307 37.29192943809846 24.156081789273596 19.550194524879643 108-109 19.00725446032073 37.7235489303046 23.499193554117138 19.77000305525753 110-111 18.878702886284067 38.14321468347505 23.56915873407783 19.40892369616305 112-113 19.030607713214785 38.013252091734316 23.687136944202656 19.26900325084824 114-115 18.76231090713069 38.40985263322422 23.418653433200063 19.409183026445028 116-117 18.752530957607792 38.431481205197606 23.474498603977068 19.341489233217533 118-119 18.700703474738862 38.40758900021317 23.45146734882399 19.440240176223973 120-121 18.651712138356626 38.35786773626834 23.599922550985788 19.390497574389247 122-123 18.818762811153388 38.33292479741948 23.528826649330533 19.319485742096607 124-125 18.790154552285067 38.56524465301671 23.343416562903542 19.30118423179468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7423.0 1 6824.5 2 5634.0 3 4018.0 4 2223.5 5 1189.5 6 816.5 7 695.5 8 744.5 9 762.0 10 546.5 11 353.5 12 333.5 13 327.5 14 350.5 15 364.0 16 334.5 17 370.0 18 442.0 19 496.5 20 570.0 21 678.5 22 815.5 23 1031.0 24 1237.0 25 1470.0 26 1802.5 27 2175.0 28 2542.5 29 2946.5 30 3542.5 31 4108.5 32 4855.5 33 5763.0 34 6467.0 35 6864.5 36 7684.0 37 8505.5 38 8769.0 39 8831.5 40 8409.5 41 8205.0 42 8255.5 43 8424.0 44 8702.0 45 8944.5 46 8701.0 47 8178.0 48 8094.5 49 8253.5 50 8625.5 51 8355.0 52 7532.5 53 7148.5 54 6958.0 55 6688.0 56 6109.5 57 5508.0 58 5199.5 59 4716.0 60 4052.5 61 3495.0 62 2927.5 63 2097.5 64 1442.0 65 1175.5 66 951.0 67 735.5 68 520.5 69 362.5 70 298.5 71 241.5 72 214.5 73 228.0 74 185.5 75 124.0 76 84.5 77 57.0 78 37.5 79 24.0 80 17.0 81 9.0 82 8.5 83 8.5 84 7.5 85 7.0 86 7.0 87 6.5 88 5.0 89 3.0 90 2.5 91 2.5 92 2.5 93 2.5 94 1.5 95 1.0 96 0.5 97 1.0 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04581665524210018 2 0.20599736465440388 3 0.027347925997222584 4 0.11827090074123534 5 0.02379624729628459 6 0.04155464080097458 7 0.041199472930880776 8 0.10157801084682676 9 0.13034660832442455 10-11 0.050256253618272684 12-13 0.03622712274956758 14-15 0.013496379063564394 16-17 0.03178752437339508 18-19 0.05203209296874168 20-21 0.19640783216187133 22-23 0.11276579875478146 24-25 0.07653867600521387 26-27 0.01704805776450239 28-29 0.006215437726641497 30-31 0.009411948557485696 32-33 0.008701612817298096 34-35 0.012430875453282994 36-37 0.005505101986453898 38-39 0.011010203972907796 40-41 0.008346444947204296 42-43 0.012786043323376795 44-45 0.012430875453282994 46-47 0.020422152530393492 48-49 0.02202040794581559 50-51 0.013851546933658193 52-53 0.01704805776450239 54-55 0.016870473829455494 56-57 0.01971181679020589 58-59 0.02077732040048729 60-61 0.011898123648142295 62-63 0.020599736465440388 64-65 0.03569437094442688 66-67 0.029301349282738486 68-69 0.03889088177527108 70-71 0.028946181412644684 72-73 0.018468729244877594 74-75 0.012963627258423696 76-77 0.013673962998611292 78-79 0.027525509932269487 80-81 0.02273074368600319 82-83 0.024506583036472192 84-85 0.020422152530393492 86-87 0.03160994043834819 88-89 0.03214269224348889 90-91 0.03160994043834819 92-93 0.03711504242480208 94-95 0.02983410108787919 96-97 0.03178752437339508 98-99 0.03658229061966138 100-101 0.025039334841612887 102-103 0.033030611918723383 104-105 0.029301349282738486 106-107 0.03622712274956758 108-109 0.026637590257034986 110-111 0.025039334841612887 112-113 0.032675444048629584 114-115 0.017225641699549293 116-117 0.015272218414033395 118-119 0.03445128339909859 120-121 0.028946181412644684 122-123 0.02273074368600319 124-125 0.023618663361237687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 281557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.07555628555549 #Duplication Level Percentage of deduplicated Percentage of total 1 81.46457890606065 51.384236320683065 2 10.836565768419767 13.670448281361697 3 3.390867425391866 6.4164254744148375 4 1.5037713797414372 3.794048652139539 5 0.7791364308036504 2.457223189764123 6 0.46431741513640834 1.7572247551680094 7 0.30853364757854296 1.3622652017679688 8 0.20945056916510538 1.056896893153219 9 0.1661210514373839 0.943035995713893 >10 0.8219480973489823 9.161542238878077 >50 0.035532850120859806 1.472468491609531 >100 0.01579237783149325 1.8637986053496818 >500 0.001692040481945705 0.7638052310910034 >1k 0.0011280269879638035 1.0761578127854146 >5k 5.640134939819018E-4 2.820422856119923 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7928 2.815770874103645 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1675 0.5949061824071148 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1350 0.47947662462662977 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 812 0.2883963105161655 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 793 0.2816481209843833 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 542 0.1925009855908395 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 430 0.15272218414033392 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 412 0.14632916247864552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 341 0.12111224370198574 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 313 0.11116754333935935 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 297 0.10548485741785854 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.7758393504689992E-4 0.0 22-23 0.0 0.0 0.0 0.0023085911556096988 0.0 24-25 0.0 0.0 0.0 0.0031965108308441986 0.0 26-27 0.0 0.0 0.0 0.0039068465710317985 0.0 28-29 0.0 0.0 0.0 0.006748189531782197 0.0 30-31 0.0 0.0 0.0 0.012253291518236094 0.0 32-33 0.0 0.0 0.0 0.02290832762105009 0.0 34-35 0.0 0.0 0.0 0.03853571390517728 0.0 36-37 0.0 0.0 0.0 0.06321988087669636 0.0 38-39 0.0 0.0 0.0 0.10512968954776475 0.0 40-41 0.0 0.0 0.0 0.15662903071136575 0.0 42-43 0.0 0.0 0.0 0.202268102018419 0.0 44-45 0.0 0.0 0.0 0.25501053072734825 0.0 46-47 0.0 0.0 0.0 0.31432356503301284 0.0 48-49 0.0 0.0 0.0 0.3855347229868197 0.0 50-51 0.0 0.0 0.0 0.46367165440745567 0.0 52-53 0.0 0.0 0.0 0.5435844251785606 0.0 54-55 0.0 0.0 0.0 0.6240299477548064 0.0 56-57 0.0 0.0 0.0 0.7083823169020838 0.0 58-59 0.0 0.0 0.0 0.8131568385797547 0.0 60-61 0.0 0.0 0.0 0.9468775416700703 0.0 62-63 0.0 0.0 0.0 1.0647932745412119 0.0 64-65 0.0 0.0 0.0 1.1704557158941173 0.0 66-67 0.0 0.0 0.0 1.298848900933026 0.0 68-69 0.0 0.0 0.0 1.4522814208135477 0.0 70-71 0.0 0.0 0.0 1.6339497863665262 0.0 72-73 0.0 0.0 0.0 1.8102906338680977 0.0 74-75 0.0 0.0 0.0 1.9789953721626528 0.0 76-77 0.0 0.0 0.0 2.151962124898333 0.0 78-79 0.0 0.0 0.0 2.3684369417205042 0.0 80-81 0.0 0.0 0.0 2.5950340428403487 0.0 82-83 0.0 0.0 0.0 2.848446318152275 0.0 84-85 0.0 0.0 0.0 3.0926242288417622 0.0 86-87 0.0 0.0 0.0 3.334315964440593 0.0 88-89 0.0 0.0 0.0 3.602290122426365 0.0 90-91 0.0 0.0 0.0 3.9134171766285335 0.0 92-93 0.0 0.0 0.0 4.252424908633065 0.0 94-95 0.0 0.0 0.0 4.575094918613282 0.0 96-97 0.0 0.0 0.0 4.895989089243031 0.0 98-99 0.0 0.0 0.0 5.241034675039157 0.0 100-101 0.0 0.0 0.0 5.6441502075956205 0.0 102-103 0.0 0.0 0.0 6.0902410524334325 0.0 104-105 0.0 0.0 0.0 6.508451219468881 0.0 106-107 0.0 0.0 0.0 6.923464875673487 0.0 108-109 0.0 0.0 0.0 7.362452363109424 0.0 110-111 0.0 0.0 0.0 7.849565096943071 0.0 112-113 0.0 0.0 0.0 8.371661865980958 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGGC 25 0.0020347182 71.58073 2 ACTGCGC 30 0.0042088265 59.58692 8 GTACAAG 175 0.0 51.1382 1 ATCGCGT 25 5.331623E-4 47.59755 24-25 GTATCAA 1030 0.0 47.497295 1 CGCGTCA 30 0.0013111344 39.647015 26-27 GGTATCA 605 0.0 38.45931 1 GAGAACA 590 0.0 37.414673 1 GTACATG 2345 0.0 36.63632 1 GAACAAA 525 0.0 35.228535 1 GTAGCAC 85 1.6873097E-4 35.069855 3 CATCGCG 35 0.0027703163 34.04361 22-23 GCGTCAA 35 0.0027945656 33.983154 26-27 CGTCAAC 35 0.0027945656 33.983154 28-29 TACATGG 2340 0.0 33.903976 2 ATCAACG 1235 0.0 33.792004 3 TCAACGC 1235 0.0 33.792004 4 GAAAACA 645 0.0 33.299294 1 CAACGCA 1270 0.0 32.843185 5 AACGCAG 1290 0.0 32.33399 6 >>END_MODULE