FastQCFastQC Report
Fri 27 May 2016
SRR940007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281557
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88473.142170146719847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62252.210919991333904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47901.7012540977493014No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43351.5396527168566223No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.7082047329670369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.6002337004585218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.502207368312633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11900.4226497654116218No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.2329901227815327No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.1861079639291511No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.14774983395902075No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.11578472565057873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG357.408744E-9102.174912
TAACGCA1050.085.145754
TATAACG1200.074.502532
GGTATCA19650.073.403011
ATCACGC508.8004526E-871.522433
ATAACGC1400.063.8593183
GTATAAC1700.059.602031
GTATCAA39150.058.0035061
GTATAGG655.402235E-755.0172541
GTATCAC909.559335E-846.3571321
TATCAAC50750.044.8630072
TCACGCA802.2608601E-644.701524
ATCAACG51500.044.2096633
TCAACGC51950.043.9414374
CAACGCA52750.043.3340535
AACGCAG54000.042.8592876
GTATAGT853.4308232E-642.072021
CACGCAG853.4603218E-642.019695
TGGTATC8100.039.7346842
GCGTAAA300.001308356939.6641268-69
Produced by FastQC (version 0.11.5)