##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940007_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 281557 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.08161757654756 34.0 33.0 34.0 31.0 34.0 2 33.08926789246937 34.0 33.0 34.0 31.0 34.0 3 33.15961954417755 34.0 33.0 34.0 31.0 34.0 4 36.411085499561366 37.0 37.0 37.0 35.0 37.0 5 36.45862116729472 37.0 37.0 37.0 35.0 37.0 6 36.56626544536275 37.0 37.0 37.0 35.0 37.0 7 36.549966791804145 37.0 37.0 37.0 35.0 37.0 8 36.56648920112091 37.0 37.0 37.0 35.0 37.0 9 38.41663322169223 39.0 39.0 39.0 37.0 39.0 10-11 38.41500122532915 39.0 39.0 39.0 37.0 39.0 12-13 38.38661798499061 39.0 39.0 39.0 37.0 39.0 14-15 39.84269970201416 41.0 40.0 41.0 37.5 41.0 16-17 39.887825555748925 41.0 40.0 41.0 38.0 41.0 18-19 40.03125122088955 41.0 40.0 41.0 38.0 41.0 20-21 40.004210515099956 41.0 40.0 41.0 37.5 41.0 22-23 39.86624200428332 41.0 40.0 41.0 36.5 41.0 24-25 39.796428431898335 41.0 40.0 41.0 36.0 41.0 26-27 39.627325905589274 41.0 40.0 41.0 35.5 41.0 28-29 39.460839190643455 41.0 39.5 41.0 35.0 41.0 30-31 39.0119389679532 41.0 38.5 41.0 35.0 41.0 32-33 38.49524430221945 41.0 36.0 41.0 35.0 41.0 34-35 38.21903202548685 41.0 35.0 41.0 35.0 41.0 36-37 38.0337125342293 41.0 35.0 41.0 35.0 41.0 38-39 37.86740162737918 41.0 35.0 41.0 35.0 41.0 40-41 37.642866275745234 40.0 35.0 41.0 35.0 41.0 42-43 37.32395394183061 40.0 35.0 41.0 33.5 41.0 44-45 36.82195612256133 40.0 35.0 41.0 33.0 41.0 46-47 36.05429451230124 40.0 35.0 41.0 28.5 41.0 48-49 35.41054386855947 40.0 35.0 41.0 22.5 41.0 50-51 35.317317630177904 40.0 35.0 41.0 21.5 41.0 52-53 35.24785922566301 40.0 35.0 41.0 22.0 41.0 54-55 35.12879097305341 40.0 35.0 41.0 20.5 41.0 56-57 34.97613804664775 40.0 35.0 41.0 20.0 41.0 58-59 34.79487989998473 39.0 35.0 41.0 19.5 41.0 60-61 34.59733553063856 39.0 35.0 41.0 18.0 41.0 62-63 34.35857215412865 39.0 35.0 41.0 16.5 41.0 64-65 34.08740858866943 37.5 35.0 41.0 11.5 41.0 66-67 33.802519560870444 37.0 35.0 41.0 7.5 41.0 68-69 33.49217565182183 37.0 35.0 40.0 6.0 41.0 70-71 33.16174877555877 36.0 35.0 39.0 2.0 41.0 72-73 32.82578483220094 36.0 35.0 39.0 2.0 41.0 74-75 32.49656197501749 35.0 35.0 39.0 2.0 41.0 76-77 32.164591894358864 35.0 35.0 37.0 2.0 39.5 78-79 31.8492951693618 35.0 34.5 37.0 2.0 39.0 80-81 31.546358286243994 35.0 34.0 36.5 2.0 39.0 82-83 31.263412026694418 35.0 34.0 36.0 2.0 38.0 84-85 31.00719392520875 35.0 34.0 36.0 2.0 37.0 86-87 30.793475211058507 35.0 34.0 35.0 2.0 37.0 88-89 30.655444545864604 35.0 34.0 35.0 2.0 36.0 90-91 30.59261712548436 35.0 34.0 35.0 2.0 36.0 92-93 30.468155648767393 35.0 34.0 35.0 2.0 36.0 94-95 30.35785293919171 35.0 34.0 35.0 2.0 36.0 96-97 30.26111941809296 35.0 34.0 35.0 2.0 36.0 98-99 30.176236072979894 35.0 34.0 35.0 2.0 35.0 100-101 30.086248965573578 35.0 34.0 35.0 2.0 35.0 102-103 29.606157900531684 35.0 32.5 35.0 2.0 35.0 104-105 29.922976519852106 35.0 34.0 35.0 2.0 35.0 106-107 29.92873556686568 35.0 34.0 35.0 2.0 35.0 108-109 29.874387779383923 35.0 34.0 35.0 2.0 35.0 110-111 29.794432388468408 35.0 34.0 35.0 2.0 35.0 112-113 29.715878134800413 35.0 34.0 35.0 2.0 35.0 114-115 29.618979460642073 35.0 34.0 35.0 2.0 35.0 116-117 29.52226724961553 35.0 34.0 35.0 2.0 35.0 118-119 29.417482072901755 35.0 33.0 35.0 2.0 35.0 120-121 29.293512503684866 35.0 33.0 35.0 2.0 35.0 122-123 29.166197253131692 35.0 33.0 35.0 2.0 35.0 124-125 28.29151823609429 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13216142215765103 1101 2 0.19811011089090158 1101 3 0.19416818962607607 1101 4 0.07054079487330966 1101 5 -0.03186278075742166 1101 6 0.04815692312902087 1101 7 0.10812853603216155 1101 8 0.007765834351950218 1101 9 0.11203900284812107 1101 10-11 0.13061922073460153 1101 12-13 0.005239543059403218 1101 14-15 0.14719917340215005 1101 16-17 0.075552295263698 1101 18-19 0.05924299750076045 1101 20-21 0.1046748623134377 1101 22-23 0.18200656452894748 1101 24-25 0.2276711116029304 1101 26-27 0.3183738066887969 1101 28-29 0.2216410541149827 1101 30-31 0.1977187450890554 1101 32-33 0.2431490429709129 1101 34-35 0.1760130816764729 1101 36-37 0.23650426817833647 1101 38-39 0.3011169691768103 1101 40-41 0.3218172604973901 1101 42-43 0.02621944653972008 1101 44-45 0.013304168720274845 1101 46-47 0.41581370591736544 1101 48-49 0.3305079232559791 1101 50-51 0.25522585330788417 1101 52-53 0.09236619847089145 1101 54-55 0.14148729726396425 1101 56-57 0.14814032229594432 1101 58-59 0.16030746021614561 1101 60-61 0.03155388639594037 1101 62-63 0.10789203343507126 1101 64-65 0.07300887156087299 1101 66-67 0.3820862850199873 1101 68-69 0.3242684900056645 1101 70-71 0.3027484608359359 1101 72-73 0.4612786010763088 1101 74-75 0.483889100998411 1101 76-77 0.508312889202692 1101 78-79 0.5649615898805713 1101 80-81 0.3884320500417573 1101 82-83 0.4523866695167733 1101 84-85 0.3989567575139681 1101 86-87 0.5948927411317051 1101 88-89 0.6280312315761272 1101 90-91 0.6376586309203098 1101 92-93 0.7729098918607846 1101 94-95 0.8498896970510259 1101 96-97 0.825813297038529 1101 98-99 0.835340232502876 1101 100-101 0.5272361883254888 1101 102-103 0.5433974978382565 1101 104-105 0.6457142996939425 1101 106-107 0.7893719460636319 1101 108-109 0.5517631314484603 1101 110-111 0.6137832545592516 1101 112-113 0.6480773555306669 1101 114-115 0.5084292122426568 1101 116-117 0.36689459295001114 1101 118-119 0.38497755763645714 1101 120-121 0.5259381466084747 1101 122-123 0.5163033695422463 1101 124-125 0.5430330473721519 1102 1 0.3160364571814682 1102 2 0.19891565866338823 1102 3 0.30333742470592995 1102 4 0.20166996270743454 1102 5 0.24650651036053972 1102 6 0.10167331427499704 1102 7 0.08448746010052588 1102 8 0.24039402151922218 1102 9 0.33731218861444745 1102 10-11 0.22299453594894914 1102 12-13 0.26394296138386864 1102 14-15 0.3113382880000799 1102 16-17 0.36516423138026255 1102 18-19 0.3457378139759655 1102 20-21 0.3895060475193759 1102 22-23 0.40207871359030634 1102 24-25 0.4381992707511486 1102 26-27 0.5110223076694567 1102 28-29 0.4797490675641285 1102 30-31 0.511567162012561 1102 32-33 0.4547804523292811 1102 34-35 0.42095214023629524 1102 36-37 0.49135506672134 1102 38-39 0.5343580731274997 1102 40-41 0.47394320614042584 1102 42-43 0.346162007481162 1102 44-45 0.33974364756588926 1102 46-47 0.35744651622837864 1102 48-49 0.06555275374071101 1102 50-51 -0.09986310718525004 1102 52-53 -0.27897381273673005 1102 54-55 -0.38877327992852884 1102 56-57 -0.3960247099259675 1102 58-59 -0.40564238567905875 1102 60-61 -0.2849913643745765 1102 62-63 -0.17988115233124802 1102 64-65 -0.068715000672249 1102 66-67 0.0981131833108222 1102 68-69 0.11442329527324802 1102 70-71 -0.015705587917217656 1102 72-73 0.03279723374652832 1102 74-75 0.010839927560468254 1102 76-77 -0.02373390261575281 1102 78-79 -0.005558864028085253 1102 80-81 0.12557830949818793 1102 82-83 -0.026826695571486425 1102 84-85 0.11894134703484482 1102 86-87 0.15536135979239418 1102 88-89 -0.007265770385206594 1102 90-91 0.05943293744875078 1102 92-93 6.872804850530656E-4 1102 94-95 0.11719146225136612 1102 96-97 0.15036148980961173 1102 98-99 0.22569817590438745 1102 100-101 0.1589257372187376 1102 102-103 0.2536104426407242 1102 104-105 0.12385943838825497 1102 106-107 0.2014796793222473 1102 108-109 0.012939231196284595 1102 110-111 0.16504060956065203 1102 112-113 0.21518298736030772 1102 114-115 0.17202545768956057 1102 116-117 0.1724073416191061 1102 118-119 0.08188145228482213 1102 120-121 0.19813273893125682 1102 122-123 0.19327871277564768 1102 124-125 0.4195390714685381 1103 1 0.0044018288712095455 1103 2 -0.019645303831573813 1103 3 0.10981342954529083 1103 4 -0.08805311835185137 1103 5 -0.026413236917321115 1103 6 0.06013547316732115 1103 7 0.005562201329183836 1103 8 -0.033097039107204296 1103 9 -0.08330886260211656 1103 10-11 0.005349355145071399 1103 12-13 0.05627896084616424 1103 14-15 0.18898481307065396 1103 16-17 0.008059781857475912 1103 18-19 0.08182465360495428 1103 20-21 -0.10998145080029076 1103 22-23 -0.15015444946924106 1103 24-25 0.05375579716599077 1103 26-27 -0.0029664677035725617 1103 28-29 -0.12998510369545357 1103 30-31 0.22360843100032923 1103 32-33 0.25837468542511743 1103 34-35 0.14533545788651736 1103 36-37 0.029943922750376828 1103 38-39 0.21809412636045522 1103 40-41 0.014649282225924765 1103 42-43 0.13938018196697755 1103 44-45 0.1706096913889823 1103 46-47 0.2708001255844721 1103 48-49 0.7982285166120633 1103 50-51 0.6258644771674895 1103 52-53 0.3875556267040565 1103 54-55 0.47127175300600754 1103 56-57 0.4851589169055899 1103 58-59 0.6384831688309589 1103 60-61 0.47050332577194354 1103 62-63 0.5806965462841589 1103 64-65 0.40958349374904657 1103 66-67 0.4859845369873952 1103 68-69 0.5444962217418592 1103 70-71 0.41554357191609625 1103 72-73 0.28652757384600136 1103 74-75 0.26659506938060673 1103 76-77 0.3006208493670428 1103 78-79 0.2452056876589701 1103 80-81 0.28778089049025724 1103 82-83 0.4878174098190229 1103 84-85 0.43759976886573426 1103 86-87 0.36084092693851844 1103 88-89 0.46724357226837654 1103 90-91 0.591720543309755 1103 92-93 0.6871622771243864 1103 94-95 0.7358061954144262 1103 96-97 0.7432083106188614 1103 98-99 0.7666899086641656 1103 100-101 0.8798895764444374 1103 102-103 0.907411356742081 1103 104-105 0.782214055944376 1103 106-107 0.6671924767412563 1103 108-109 0.9438691276877513 1103 110-111 0.9092686644067349 1103 112-113 0.8280341770363435 1103 114-115 1.0009339131271204 1103 116-117 1.0524315384197536 1103 118-119 0.833024078981957 1103 120-121 0.7846783095724703 1103 122-123 0.8427275634276157 1103 124-125 0.7172269327402141 1104 1 0.14532068746094495 1104 2 0.14435329689138854 1104 3 0.24155369707373353 1104 4 0.0867815504026268 1104 5 0.15963230621872526 1104 6 0.10150576518311283 1104 7 0.13646956585751013 1104 8 0.05180076369467912 1104 9 0.1923487143164735 1104 10-11 0.13521975543825704 1104 12-13 0.08086625740960329 1104 14-15 0.2522562894745093 1104 16-17 0.1451722737366694 1104 18-19 0.17219046460260046 1104 20-21 0.16837056732368438 1104 22-23 0.26415648987875784 1104 24-25 0.13913251919294822 1104 26-27 0.1781399606417864 1104 28-29 0.2868037220778987 1104 30-31 0.14643580352452545 1104 32-33 0.212894017103757 1104 34-35 -0.12240537601596202 1104 36-37 -0.043867941119955844 1104 38-39 0.1499460028909141 1104 40-41 0.1407341383835785 1104 42-43 0.3553269428072099 1104 44-45 0.35190352290936744 1104 46-47 0.21056041610103904 1104 48-49 0.46903609936097723 1104 50-51 0.58141244405126 1104 52-53 0.42327050734905214 1104 54-55 0.5258185704836507 1104 56-57 0.41329604605553527 1104 58-59 0.36496615262266374 1104 60-61 0.2513249012912553 1104 62-63 0.223547739834153 1104 64-65 0.09551243046507096 1104 66-67 0.28343085638903887 1104 68-69 0.08620383947270227 1104 70-71 0.11417427938391 1104 72-73 0.13347748305957197 1104 74-75 0.2543180791588924 1104 76-77 0.26182839213661424 1104 78-79 0.22901405333670155 1104 80-81 0.2565095473508805 1104 82-83 0.4468052660932784 1104 84-85 0.3931019260845048 1104 86-87 0.32868670661901334 1104 88-89 0.37319633001020946 1104 90-91 0.49317146033595805 1104 92-93 0.5728213532683739 1104 94-95 0.47039332539726786 1104 96-97 0.4909594725185471 1104 98-99 0.3835503640955302 1104 100-101 0.23725493767447148 1104 102-103 0.20843083001422258 1104 104-105 0.3823206675283899 1104 106-107 0.39155155788266427 1104 108-109 0.2986816412224229 1104 110-111 0.11173905470507961 1104 112-113 0.032386928114952696 1104 114-115 0.16756865184544978 1104 116-117 0.2912331575831857 1104 118-119 0.44165961728716496 1104 120-121 0.5278651630315174 1104 122-123 0.20426993320323206 1104 124-125 0.16089353832732556 1105 1 -0.003533901509896964 1105 2 0.0827844012727681 1105 3 -0.08026854034465458 1105 4 0.15584812589229813 1105 5 0.1867574629291866 1105 6 0.15009007948324893 1105 7 0.09425950056827048 1105 8 0.1015817760849842 1105 9 0.11506806826232463 1105 10-11 0.06049125122946464 1105 12-13 0.03722341073349611 1105 14-15 0.0763905150398827 1105 16-17 0.16660293851467145 1105 18-19 0.16474977035488791 1105 20-21 0.09116318849954297 1105 22-23 0.1595268150224456 1105 24-25 0.07213370775023265 1105 26-27 0.044353998643046566 1105 28-29 0.12285608372494039 1105 30-31 0.06900048220701649 1105 32-33 -0.031891798360511814 1105 34-35 0.07118699767946879 1105 36-37 -0.10395928093336693 1105 38-39 0.2924466948592013 1105 40-41 0.11072713729270589 1105 42-43 0.20426212573106284 1105 44-45 0.04002120636236839 1105 46-47 0.13739494613245995 1105 48-49 0.06281001297077182 1105 50-51 0.0710654830131503 1105 52-53 0.15632542520834392 1105 54-55 0.08404566633346633 1105 56-57 -6.922963140922889E-4 1105 58-59 6.774146099957079E-4 1105 60-61 -0.05333652269806066 1105 62-63 0.007405340902224111 1105 64-65 -0.10838620025180745 1105 66-67 -0.11793924081215579 1105 68-69 -0.004146866334949095 1105 70-71 -0.005646134494533328 1105 72-73 -0.11422681040010474 1105 74-75 -0.09097816467445341 1105 76-77 -0.01629401298232125 1105 78-79 0.15071987726740943 1105 80-81 -0.32184975514508807 1105 82-83 -0.1685377059512021 1105 84-85 -0.018688399384810594 1105 86-87 0.03948466816185814 1105 88-89 -0.12477660995950757 1105 90-91 0.06891756496156276 1105 92-93 -0.06117219921538464 1105 94-95 -0.0870359717257756 1105 96-97 -0.10211026160281733 1105 98-99 -0.1799071978966893 1105 100-101 -0.0578329015935104 1105 102-103 -0.16708000539501455 1105 104-105 -0.12485952101261333 1105 106-107 -0.3050651242515272 1105 108-109 -0.28364630289910053 1105 110-111 -0.3744663540446531 1105 112-113 -0.2940470743924166 1105 114-115 -0.22765920923988503 1105 116-117 -0.2740845779709602 1105 118-119 -0.707732264703747 1105 120-121 -0.8775061390085668 1105 122-123 -0.8447254916652973 1105 124-125 -0.7315908720342854 1106 1 0.06128390664601824 1106 2 0.0786048418583718 1106 3 0.10978980414956396 1106 4 0.018269037544264677 1106 5 0.11177170268041436 1106 6 0.001457865317391338 1106 7 0.04077191609190578 1106 8 0.11036423886920232 1106 9 0.10120547801308533 1106 10-11 0.2052556410753681 1106 12-13 0.23520983293826703 1106 14-15 0.2981380788878525 1106 16-17 0.3204268890065194 1106 18-19 0.36519158750108716 1106 20-21 0.3528282387620081 1106 22-23 0.32282597295282756 1106 24-25 0.32253011163576417 1106 26-27 0.2646698902054254 1106 28-29 0.29410256227295406 1106 30-31 0.12454320915786354 1106 32-33 0.036528281997867396 1106 34-35 0.3278219520352934 1106 36-37 0.47989745947212015 1106 38-39 0.35679882466415336 1106 40-41 0.4527428702935268 1106 42-43 0.5279470654622784 1106 44-45 0.4215310818222733 1106 46-47 -0.08469514146125334 1106 48-49 -0.3007500787794086 1106 50-51 -0.30307820762248383 1106 52-53 -0.16760967231470403 1106 54-55 -0.283543890156011 1106 56-57 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-0.22449028280145455 2216 8 -0.26067603127005157 2216 9 -0.23534439577705513 2216 10-11 -0.3398978167404536 2216 12-13 -0.37741992918540035 2216 14-15 -0.3933895619071919 2216 16-17 -0.38169694710454394 2216 18-19 -0.45847819401376455 2216 20-21 -0.4374753335620838 2216 22-23 -0.3765897840555752 2216 24-25 -0.5091719662298786 2216 26-27 -0.38449595456481234 2216 28-29 -0.3823300450054319 2216 30-31 -0.42339146261291916 2216 32-33 -0.5410552588991422 2216 34-35 -0.5598136608765572 2216 36-37 -0.4209630243044842 2216 38-39 -0.3871268034597506 2216 40-41 -0.48850685806274186 2216 42-43 -0.5099158923619029 2216 44-45 -0.46797678330869985 2216 46-47 -0.5326858136332291 2216 48-49 -0.5128286130733883 2216 50-51 -0.3925492963705821 2216 52-53 -0.30527036003306307 2216 54-55 -0.28503166200870567 2216 56-57 -0.264140703648323 2216 58-59 -0.29722820391275917 2216 60-61 -0.1580296009275841 2216 62-63 -0.27401904646735886 2216 64-65 -0.13442515904005603 2216 66-67 -0.10382361223775405 2216 68-69 -0.04967821706802056 2216 70-71 -0.19871136620228214 2216 72-73 -0.08642448119140766 2216 74-75 0.0026451066159722814 2216 76-77 -0.02539248443911646 2216 78-79 -0.31961044057252863 2216 80-81 -0.3647555469720629 2216 82-83 -0.2188565857127358 2216 84-85 -0.2707897130307835 2216 86-87 -0.1716735239285363 2216 88-89 -0.3527323134013294 2216 90-91 -0.41769468418058153 2216 92-93 -0.3158254174747128 2216 94-95 -0.32488301342277026 2216 96-97 -0.3431201052824129 2216 98-99 -0.4104927928500679 2216 100-101 -0.43718283747094944 2216 102-103 -0.554475589603534 2216 104-105 -0.4110064483288234 2216 106-107 -0.4058198641218951 2216 108-109 -0.34876536289702287 2216 110-111 -0.2943954726194029 2216 112-113 -0.1933096649438717 2216 114-115 -0.1936829172569361 2216 116-117 -0.47911213334165836 2216 118-119 -0.45357296346636033 2216 120-121 -0.5334712701998612 2216 122-123 -0.5893400630839629 2216 124-125 -0.7138965113535498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 15.0 5 34.0 6 143.0 7 218.0 8 350.0 9 571.0 10 1235.0 11 1726.0 12 2081.0 13 3335.0 14 6478.0 15 6811.0 16 2421.0 17 1584.0 18 1414.0 19 1234.0 20 1066.0 21 1003.0 22 1069.0 23 1079.0 24 1257.0 25 1367.0 26 1577.0 27 1836.0 28 1948.0 29 2197.0 30 2755.0 31 3337.0 32 4086.0 33 5449.0 34 8323.0 35 26843.0 36 27805.0 37 86891.0 38 71175.0 39 844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.70373922743 18.276246705174387 14.316416934870801 18.703597132524813 2 15.498735579928397 21.610572824913337 42.50369381144513 20.38699778371313 3 18.310744576567362 30.973944792510196 30.032036255664945 20.683274375257497 4 10.838359984066466 18.051840892277927 38.5374580322085 32.57234109144711 5 10.766175023184585 38.731225816079615 33.51549369841208 16.98710546232372 6 25.9957445750436 39.4703097082654 17.998174216671462 16.535771500019536 7 23.37572853809353 37.14665236524043 22.11949978157176 17.358119315094278 8 26.017467155851214 33.08530777071783 20.23142738415312 20.665797689277838 9 28.009958907077433 15.003001168502292 21.652809200268507 35.33423072415177 10-11 23.630739068820876 28.246323124624855 26.62444904584152 21.49848876071275 12-13 26.980682419545598 26.533703655032554 28.6160528773925 17.86956104802935 14-15 23.072415177033427 25.013407587096044 31.71666838331137 20.197508852559164 16-17 17.966166708694864 28.619959723963532 31.54618780566635 21.867685761675258 18-19 17.593240445096374 23.124092102132074 40.695134555347586 18.587532897423966 20-21 19.035754749482344 21.901426709334167 41.80219280642996 17.260625734753532 22-23 18.229878852239512 22.277904651633595 41.8886761827978 17.603540313329095 24-25 17.920882805257904 22.59027479338109 42.11598361965783 17.372858781703172 26-27 17.665517106660463 22.61957614266383 42.2237770682313 17.491129682444406 28-29 17.681416274944016 22.364507175317122 42.22116065926611 17.732915890472746 30-31 17.879423417407935 22.262395868665326 42.27834299099112 17.579837722935622 32-33 17.785417846861996 22.347379655593105 41.99352527823012 17.873677219314775 34-35 18.0104810596845 22.446551390400828 41.74310215925457 17.7998653906601 36-37 18.17182494299495 22.152588846189364 41.503761267820735 18.17182494299495 38-39 18.025345589384404 22.648003182405844 41.22654041229204 18.10011081591771 40-41 18.183351861257226 22.507165511779142 41.109970627617145 18.19951199934649 42-43 18.20745502654899 22.59434213563957 40.60503276446875 18.593170073342684 44-45 18.433100342383042 22.8908281123471 39.8822969498075 18.79377459546236 46-47 18.595557197223293 23.355170276658505 38.68708411988357 19.36218840623463 48-49 18.898707516577815 23.913251950801097 37.4409964802114 19.747044052409688 50-51 19.174357708475036 24.129241468226837 36.72538319058215 19.971017632715977 52-53 19.15171705906797 24.54547391824746 36.32656975319385 19.976239269490726 54-55 19.284549842483052 24.41317388663752 36.176333744144166 20.125942526735262 56-57 19.2636844899938 24.669464270175332 35.823946325792356 20.242904914038512 58-59 19.270398613395088 24.116412301856503 36.364637312600564 20.248551772147852 60-61 19.65043943690986 24.320244642670044 36.33359201714772 19.69572390327238 62-63 19.7856917071854 24.150349662768107 36.56488739402678 19.499071236019706 64-65 19.759972864644656 23.967196054718233 36.61355027410616 19.659280806530955 66-67 19.832644305218146 24.29925130347071 36.194043103325804 19.67406128798534 68-69 20.082435194607086 24.266349854113503 36.124799994317264 19.526414956962153 70-71 19.96046904385166 24.32663296057737 36.11772624191311 19.595171753657855 72-73 19.761362708083976 24.384760457030016 35.91794911865093 19.935927716235078 74-75 19.657688598913538 24.738196418836004 35.68466719823552 19.91944778401493 76-77 19.54703350098031 24.686727474213622 36.055110391270986 19.711128633535075 78-79 19.344246574369393 24.659212797545056 36.0078350866974 19.988705541388153 80-81 19.028651392080466 24.94113092553195 35.71035349858111 20.319864183806477 82-83 19.40299832715933 24.735843896617737 35.4040212106252 20.45713656559773 84-85 19.22107719437198 24.519677292754004 35.37792726478439 20.88131824808963 86-87 19.084235163750147 24.4888246429675 35.51696459331503 20.909975599967325 88-89 19.23749009969562 24.48474021246142 35.48393398139631 20.793835706446654 90-91 19.212805932724102 24.695887511232183 35.37507502921255 20.71623152683116 92-93 19.146381961574047 24.60997781956957 35.367752957242736 20.87588726161365 94-95 18.97014061295742 24.508707064034123 35.398663839490254 21.1224884835182 96-97 19.414925733607053 24.459428741910965 35.225036760266235 20.900608764215743 98-99 19.24050902659142 24.48474021246142 35.13125228639316 21.143498474553997 100-101 19.03330237447901 24.549957113403526 35.372829254519075 21.043911257598385 102-103 19.491079958942596 24.385825960640297 35.155226117624494 20.967867962792614 104-105 19.35380757715134 24.490955650188063 35.03802072049354 21.117216052167056 106-107 19.208933198132524 24.54693818114659 35.086918611362194 21.15721000935869 108-109 19.23968944011138 24.667206523746607 34.7145860858941 21.378517950247907 110-111 19.14372578199086 24.905436554587528 34.69190963108713 21.258928032334484 112-113 19.17969991706786 24.81873023258292 34.55109205822653 21.45047779212269 114-115 18.908863596406327 24.95946553105038 34.46859444398074 21.66307642856255 116-117 18.787193197740393 24.887809954413637 34.58241506498765 21.74258178285832 118-119 18.72025458523349 24.99183110997457 34.31608632030573 21.97182798448621 120-121 18.630718878804075 25.13225587049136 34.285836057771704 21.95118919293286 122-123 18.66069747866329 25.20022588676538 34.07409512105897 22.06498151351236 124-125 18.5196826902074 25.290663086542747 34.036394374801326 22.153259848448524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1157.0 1 846.5 2 2389.0 3 3186.5 4 1234.5 5 511.0 6 1355.5 7 3607.0 8 6074.0 9 5975.5 10 3319.0 11 1245.5 12 781.5 13 713.0 14 731.5 15 768.5 16 799.0 17 869.0 18 926.0 19 945.5 20 1043.0 21 1185.0 22 1245.0 23 1368.0 24 1577.5 25 1758.5 26 2070.5 27 2399.5 28 2613.5 29 3012.5 30 3560.5 31 4028.0 32 4524.5 33 5170.0 34 5948.0 35 6509.0 36 7210.5 37 7925.0 38 8176.5 39 8340.0 40 8039.5 41 7798.5 42 7955.0 43 8181.5 44 8467.0 45 8728.5 46 8700.5 47 8453.5 48 8439.5 49 8487.0 50 8653.0 51 8288.5 52 7411.5 53 6860.5 54 6582.5 55 6273.5 56 5723.0 57 5125.0 58 4741.5 59 4318.0 60 3730.0 61 3275.5 62 2785.0 63 1983.5 64 1321.5 65 1078.0 66 871.5 67 641.0 68 452.0 69 319.5 70 277.0 71 232.0 72 198.0 73 197.5 74 159.5 75 113.0 76 75.0 77 42.0 78 25.5 79 12.0 80 7.5 81 2.5 82 4.0 83 3.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01953423285515899 2 0.0017758393504689993 3 3.5516787009379984E-4 4 0.13816030146648814 5 0.04262014441125598 6 0.012075707583189195 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.327518051406997E-4 30-31 5.327518051406997E-4 32-33 1.7758393504689992E-4 34-35 5.327518051406997E-4 36-37 0.0010655036102813994 38-39 0.0031965108308441986 40-41 0.0 42-43 7.103357401875997E-4 44-45 3.5516787009379984E-4 46-47 1.7758393504689992E-4 48-49 0.002131007220562799 50-51 0.002308591155609699 52-53 0.0 54-55 0.0 56-57 0.002308591155609699 58-59 0.002131007220562799 60-61 0.0012430875453282996 62-63 0.0 64-65 0.001953423285515899 66-67 3.5516787009379984E-4 68-69 8.879196752344996E-4 70-71 0.001953423285515899 72-73 0.0 74-75 1.7758393504689992E-4 76-77 0.004617182311219398 78-79 0.0010655036102813994 80-81 0.0 82-83 0.0 84-85 5.327518051406997E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 8.879196752344996E-4 94-95 0.0014206714803751993 96-97 0.0010655036102813994 98-99 0.0 100-101 1.7758393504689992E-4 102-103 0.0 104-105 0.0 106-107 1.7758393504689992E-4 108-109 3.5516787009379984E-4 110-111 0.0 112-113 5.327518051406997E-4 114-115 0.002308591155609699 116-117 0.0012430875453282996 118-119 3.5516787009379984E-4 120-121 1.7758393504689992E-4 122-123 0.0 124-125 8.879196752344996E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 281557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.694828708379674 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23818212120983 42.792239695745856 2 12.573225290290333 13.753808071285942 3 3.9735660291223787 6.520005399728547 4 1.7719112580516643 3.876575309823413 5 0.9837199951379147 2.690219831553817 6 0.5698659900051152 1.8701233626036584 7 0.3494755441155811 1.3380153516218647 8 0.27038950089182734 1.1831125948658214 9 0.18059863527233597 0.8890030279068798 >10 1.0117877437202358 9.843069054468247 >50 0.048704553896714714 1.8174814626293403 >100 0.022079397766510668 2.179414941312025 >500 0.0012987881039123925 0.4191182579446202 >1k 0.0038963643117371767 5.474465855678331 >5k 0.0012987881039123925 5.353347782831624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8847 3.142170146719847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6225 2.210919991333904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4790 1.7012540977493014 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4335 1.5396527168566223 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1994 0.7082047329670369 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1690 0.6002337004585218 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1414 0.502207368312633 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1190 0.4226497654116218 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 656 0.2329901227815327 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 524 0.1861079639291511 No Hit GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.14774983395902075 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.11578472565057873 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.7758393504689992E-4 0.0 22-23 0.0 0.0 0.0 0.0019534232855158993 0.0 24-25 0.0 0.0 0.0 0.0028413429607503987 0.0 26-27 0.0 0.0 0.0 0.0031965108308441986 0.0 28-29 0.0 0.0 0.0 0.0060378537915945964 0.0 30-31 0.0 0.0 0.0 0.011542955778048494 0.0 32-33 0.0 0.0 0.0 0.02255315975095629 0.0 34-35 0.0 0.0 0.0 0.03853571390517728 0.0 36-37 0.0 0.0 0.0 0.06357504874679017 0.0 38-39 0.0 0.0 0.0 0.10637277709309305 0.0 40-41 0.0 0.0 0.0 0.15858245399688162 0.0 42-43 0.0 0.0 0.0 0.2042215253039349 0.0 44-45 0.0 0.0 0.0 0.2578518736880987 0.0 46-47 0.0 0.0 0.0 0.317520075863857 0.0 48-49 0.0 0.0 0.0 0.3892639856228046 0.0 50-51 0.0 0.0 0.0 0.4688215885238158 0.0 52-53 0.0 0.0 0.0 0.5489119432299676 0.0 54-55 0.0 0.0 0.0 0.630067801546401 0.0 56-57 0.0 0.0 0.0 0.7153080903689129 0.0 58-59 0.0 0.0 0.0 0.8209705317218183 0.0 60-61 0.0 0.0 0.0 0.9561119062925092 0.0 62-63 0.0 0.0 0.0 1.0775793178645887 0.0 64-65 0.0 0.0 0.0 1.1857279343081508 0.0 66-67 0.0 0.0 0.0 1.3158969586975284 0.0 68-69 0.0 0.0 0.0 1.4718156536687066 0.0 70-71 0.0 0.0 0.0 1.6570356979226233 0.0 72-73 0.0 0.0 0.0 1.8355075526447575 0.0 74-75 0.0 0.0 0.0 2.006520882094922 0.0 76-77 0.0 0.0 0.0 2.182328977791353 0.0 78-79 0.0 0.0 0.0 2.402177889379415 0.0 80-81 0.0 0.0 0.0 2.630550829849729 0.0 82-83 0.0 0.0 0.0 2.886804448122405 0.0 84-85 0.0 0.0 0.0 3.1348892053829243 0.0 86-87 0.0 0.0 0.0 3.3808429554228807 0.0 88-89 0.0 0.0 0.0 3.653967047525013 0.0 90-91 0.0 0.0 0.0 3.9718422912589633 0.0 92-93 0.0 0.0 0.0 4.3177757967303245 0.0 94-95 0.0 0.0 0.0 4.64968017133298 0.0 96-97 0.0 0.0 0.0 4.981229378065542 0.0 98-99 0.0 0.0 0.0 5.338350671444859 0.0 100-101 0.0 0.0 0.0 5.750522984688713 0.0 102-103 0.0 0.0 0.0 6.203539602993354 0.0 104-105 0.0 0.0 0.0 6.6334703097419 0.0 106-107 0.0 0.0 0.0 7.057718330568943 0.0 108-109 0.0 0.0 0.0 7.508426357717976 0.0 110-111 0.0 0.0 0.0 8.005483791914248 0.0 112-113 0.0 0.0 0.0 8.538590764925042 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 35 7.408744E-9 102.17491 2 TAACGCA 105 0.0 85.14575 4 TATAACG 120 0.0 74.50253 2 GGTATCA 1965 0.0 73.40301 1 ATCACGC 50 8.8004526E-8 71.52243 3 ATAACGC 140 0.0 63.859318 3 GTATAAC 170 0.0 59.60203 1 GTATCAA 3915 0.0 58.003506 1 GTATAGG 65 5.402235E-7 55.017254 1 GTATCAC 90 9.559335E-8 46.357132 1 TATCAAC 5075 0.0 44.863007 2 TCACGCA 80 2.2608601E-6 44.70152 4 ATCAACG 5150 0.0 44.209663 3 TCAACGC 5195 0.0 43.941437 4 CAACGCA 5275 0.0 43.334053 5 AACGCAG 5400 0.0 42.859287 6 GTATAGT 85 3.4308232E-6 42.07202 1 CACGCAG 85 3.4603218E-6 42.01969 5 TGGTATC 810 0.0 39.734684 2 GCGTAAA 30 0.0013083569 39.66412 68-69 >>END_MODULE