FastQCFastQC Report
Fri 27 May 2016
SRR940006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603214
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20090.12531078196672435No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18610.11607932565459135No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17430.10871911048680961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.051.987541
GTATCAA25850.050.1067661
ATCAACG31300.040.3868683
TCAACGC32400.039.204654
CAACGCA32250.039.1702235
TATCAAC34000.037.03792
AACGCAG34500.036.788366
GTACATG68650.033.4747541
TACATGG70800.032.0326732
ACGCAGA41900.030.148927
ACATGGG73000.030.140623
CGCAGAG41850.029.7577938
TGTACGC1107.615516E-427.1281492
ATAGCGT2006.033042E-826.814056
CATGGGG43550.026.428484
GTGGTAT10500.023.8757611
GCAGAGT52400.023.0740939
CATGGGA32350.022.305614
GTATAGG5100.022.2372261
GTACGCT1400.003117168621.2956373
Produced by FastQC (version 0.11.5)