FastQCFastQC Report
Fri 27 May 2016
SRR940006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603214
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.2526799291922351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.1843796274234132No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18610.11607932565459135No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17680.11027847810710235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.10584987406547099No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16840.10503900290291877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.075.934871
GTATCAA54600.055.8104321
ATCAACG66200.045.477683
TCAACGC66900.045.0909424
CAACGCA67800.044.5163275
TATCAAC68500.044.0377242
AACGCAG71550.042.247936
ACGCAGA81300.037.101167
CGCAGAG82550.036.539368
GCAGAGT93600.031.971449
GTGGTAT12350.031.868681
GTACATG68350.031.1470431
TAAGGTG9850.030.8230065
TACATGG69400.030.4953632
ACATGGG68050.029.7862433
TGGTATC12150.029.4401422
TACCGTA1254.887394E-528.5569157
GAGTACT61700.028.3486412-13
GTACTTT62050.027.80521614-15
CAGAGTA91600.027.37613910-11
Produced by FastQC (version 0.11.5)