FastQCFastQC Report
Fri 27 May 2016
SRR940005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627596
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34450.052.6639061
GGTATCA19850.050.2093541
ATCAACG37200.047.759563
CAACGCA38600.046.0032545
TCAACGC39450.045.039794
AACGCAG40800.043.2305876
TATCAAC45150.039.1197172
ACGCAGA50100.035.3246847
GTACATG84100.034.9848181
CGCAGAG50750.034.6374248
TACATGG84750.033.7254332
ACATGGG86300.032.0548823
GCAGAGT60650.027.8924529
CATGGGA48000.027.2038254
CCGTAAA900.00939786826.520352
CATGGGG40750.025.898394
ATACCGT2151.2151577E-724.943866
TACCGTG2451.596527E-824.3216767
CAGAGTA58350.021.72119110-11
AGAGTAC57300.020.76922410-11
Produced by FastQC (version 0.11.5)