FastQCFastQC Report
Fri 27 May 2016
SRR940005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627596
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43220.2655450123986542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.18640989533029081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.11366457032334805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.067.4872061
GTATCAA57900.060.849221
ATCAACG68000.050.5730823
TCAACGC69950.049.3336644
CAACGCA70350.048.994355
AACGCAG73850.046.7371446
TATCAAC75450.045.8164332
ACGCAGA87250.039.478847
CGCAGAG88300.038.9420138
GCAGAGT99950.034.2839559
GTACATG86900.033.8199731
TACATGG89250.032.989162
ACATGGG88900.031.2418443
GAGTACT54100.027.21630712-13
CATGGGG42650.026.8308684
CAGAGTA99250.026.46375310-11
GTGGTAT13200.026.19391
CATGGGA46200.025.9302434
AGAGTAC97600.025.72254210-11
GTACTTT57300.025.64445914-15
Produced by FastQC (version 0.11.5)