FastQCFastQC Report
Fri 27 May 2016
SRR940004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147917
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14860.1294518680357552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.056.487521
GTATCAA26050.055.1826741
TATCACG653.4717108E-545.8767972
ATCAACG34350.041.284633
TCAACGC34350.041.1147544
CAACGCA35500.039.772455
AACGCAG36450.038.89936
TATCAAC38050.038.244732
GTACATG79300.035.728421
TACATGG78500.034.6442872
ACATGGG81600.031.764153
CGCAGAG45100.031.0422828
ACGCAGA45300.031.0367437
GCAGAGT50450.027.3841749
CATGGGA49100.027.0644664
TAACGCC907.3128285E-726.43728664-65
CATGGGG35900.026.2264024
GTTCGCG2057.675408E-826.1547767
TCGCGCA1859.1929724E-725.7508229
ATGGGAT21000.023.5462175
Produced by FastQC (version 0.11.5)