##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940004_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1147917 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.577800485575178 34.0 31.0 34.0 30.0 34.0 2 31.73920152763658 34.0 31.0 34.0 30.0 34.0 3 31.81883794734288 34.0 31.0 34.0 30.0 34.0 4 34.91599392639015 37.0 37.0 37.0 33.0 37.0 5 35.11059771743079 37.0 37.0 37.0 33.0 37.0 6 35.033423148189286 37.0 37.0 37.0 33.0 37.0 7 34.99031027504602 37.0 37.0 37.0 33.0 37.0 8 34.95726084725638 37.0 37.0 37.0 33.0 37.0 9 36.567752720797756 39.0 39.0 39.0 33.0 39.0 10-11 36.61166878790017 39.0 39.0 39.0 33.0 39.0 12-13 36.56025392079741 39.0 39.0 39.0 33.0 39.0 14-15 38.08018436873049 41.0 40.0 41.0 33.0 41.0 16-17 38.14086994094521 41.0 40.0 41.0 33.0 41.0 18-19 38.17514506710851 41.0 40.0 41.0 33.0 41.0 20-21 38.205507453936136 41.0 40.0 41.0 33.5 41.0 22-23 38.26739651037488 41.0 40.0 41.0 33.0 41.0 24-25 38.3671872617968 41.0 40.0 41.0 33.0 41.0 26-27 38.43572139797564 41.0 40.0 41.0 33.0 41.0 28-29 38.400904420790006 41.0 40.0 41.0 33.0 41.0 30-31 38.35159467104329 41.0 39.0 41.0 33.0 41.0 32-33 38.30661276033023 41.0 39.0 41.0 33.0 41.0 34-35 38.22871165772438 41.0 39.0 41.0 33.0 41.0 36-37 38.0922601546976 41.0 39.0 41.0 32.0 41.0 38-39 37.98941125534337 41.0 39.0 41.0 32.0 41.0 40-41 37.88576918017592 41.0 38.5 41.0 31.5 41.0 42-43 37.72307449057728 40.5 38.0 41.0 31.0 41.0 44-45 37.573051884413246 40.0 38.0 41.0 31.0 41.0 46-47 37.40063436642196 40.0 38.0 41.0 30.0 41.0 48-49 37.245986861419425 40.0 37.5 41.0 30.0 41.0 50-51 37.06431954575113 40.0 37.0 41.0 30.0 41.0 52-53 37.1800204195948 40.0 37.0 41.0 30.0 41.0 54-55 36.95553946844589 40.0 36.5 41.0 30.0 41.0 56-57 36.82515634841195 40.0 36.0 41.0 30.0 41.0 58-59 36.611498043848115 39.5 35.5 41.0 30.5 41.0 60-61 36.34303438314791 39.0 35.0 41.0 30.5 41.0 62-63 36.026148231971476 39.0 35.0 41.0 30.0 41.0 64-65 35.693319290506196 38.0 35.0 41.0 30.0 41.0 66-67 35.35566465171262 37.0 35.0 40.0 30.0 41.0 68-69 34.99979876593865 37.0 35.0 40.0 30.0 41.0 70-71 34.634506240433765 36.0 35.0 39.0 29.5 41.0 72-73 34.26949901430156 36.0 35.0 39.0 29.0 41.0 74-75 33.90254391214696 35.0 34.0 38.0 29.0 40.0 76-77 33.54787759045297 35.0 34.0 37.0 29.0 39.0 78-79 33.21072516566964 35.0 34.0 37.0 28.0 39.0 80-81 32.90299908442858 35.0 34.0 36.0 28.0 38.5 82-83 32.6169561910835 35.0 34.0 36.0 27.5 37.0 84-85 32.380602865886644 35.0 34.0 35.5 27.0 37.0 86-87 32.17933700781502 35.0 34.0 35.0 27.0 36.5 88-89 32.01591709156672 35.0 34.0 35.0 27.0 36.0 90-91 31.87802646010121 35.0 34.0 35.0 26.0 36.0 92-93 31.732007627729182 35.0 33.0 35.0 25.5 36.0 94-95 31.621459129884826 35.0 33.0 35.0 25.0 35.5 96-97 31.52630634444825 35.0 33.0 35.0 25.0 35.0 98-99 31.4479509407039 35.0 33.0 35.0 25.0 35.0 100-101 31.350804108659425 35.0 33.0 35.0 24.5 35.0 102-103 30.659920098752785 34.0 32.0 35.0 24.0 35.0 104-105 31.565049563687968 35.0 33.5 35.0 25.0 35.0 106-107 31.67013338072352 35.0 34.0 35.0 25.0 35.0 108-109 31.649612733324794 35.0 34.0 35.0 25.0 35.0 110-111 31.602964761389543 35.0 34.0 35.0 25.0 35.0 112-113 31.550485357390823 35.0 34.0 35.0 25.0 35.0 114-115 31.48463869774557 35.0 34.0 35.0 25.0 35.0 116-117 31.402568739725957 35.0 34.0 35.0 24.5 35.0 118-119 31.315029309610363 35.0 34.0 35.0 24.0 35.0 120-121 31.22569750251978 35.0 34.0 35.0 23.5 35.0 122-123 31.092267994985697 35.0 33.5 35.0 22.5 35.0 124-125 29.81529762169216 34.0 31.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.028418942813647874 1101 2 0.10888561686812537 1101 3 0.163093707809022 1101 4 0.056324038233050544 1101 5 0.16372321101759013 1101 6 0.16985207532286495 1101 7 0.21134982385659384 1101 8 0.13435625491971592 1101 9 0.12640676559595931 1101 10-11 0.021529452280134365 1101 12-13 0.06848325290356172 1101 14-15 0.15691724131600182 1101 16-17 0.15228603845434208 1101 18-19 0.1512061788914636 1101 20-21 -0.09305824060808732 1101 22-23 -0.036495498371010626 1101 24-25 -0.008611210883991305 1101 26-27 -0.025186755769603053 1101 28-29 -0.05906131070715048 1101 30-31 0.06958449250694798 1101 32-33 0.11672770155936973 1101 34-35 0.11121625222665443 1101 36-37 0.11915937773103735 1101 38-39 0.16026551186758553 1101 40-41 0.09913522279276776 1101 42-43 0.07172404414595945 1101 44-45 0.12459068996535905 1101 46-47 0.09090223550893484 1101 48-49 0.07249014129767062 1101 50-51 0.025762194643895953 1101 52-53 0.0017282194686671914 1101 54-55 0.13528483158283677 1101 56-57 0.07233799671048757 1101 58-59 0.04910147989237856 1101 60-61 0.022990584406066716 1101 62-63 0.1426954103451692 1101 64-65 0.03611222183278073 1101 66-67 0.03556310243676819 1101 68-69 -0.022956662318044607 1101 70-71 -0.08215750443967096 1101 72-73 -0.08403386610008567 1101 74-75 -0.08817315848474294 1101 76-77 -0.09754708639473364 1101 78-79 -0.20206138814749863 1101 80-81 -0.14277562417311884 1101 82-83 -0.06875323220950946 1101 84-85 -0.05954791022101347 1101 86-87 -0.13696477974651344 1101 88-89 -0.1973585594302456 1101 90-91 -0.3426578326574514 1101 92-93 -0.23313370321690385 1101 94-95 -0.0997415621307205 1101 96-97 -0.2295776862017398 1101 98-99 -0.09050461000797583 1101 100-101 -0.23316618711949033 1101 102-103 -0.23273163917919248 1101 104-105 -0.21943435199843364 1101 106-107 -0.13096975870145044 1101 108-109 -0.11370760496516752 1101 110-111 -0.10892938740789404 1101 112-113 -0.10810353987546151 1101 114-115 -0.164620939343596 1101 116-117 -0.1478107803077826 1101 118-119 -0.27922674171366424 1101 120-121 -0.24322504911334164 1101 122-123 -0.2608275070174706 1101 124-125 -0.375960147571039 1102 1 -0.04800258923100387 1102 2 0.0578692979655564 1102 3 0.05238045423986293 1102 4 0.12298974156426823 1102 5 0.15678334241654568 1102 6 0.14908063629236779 1102 7 0.1130896076841239 1102 8 0.13783774924566927 1102 9 0.16567135531138888 1102 10-11 0.17655565500444936 1102 12-13 0.13640032898779708 1102 14-15 0.27585448964461534 1102 16-17 0.2772528033812236 1102 18-19 0.22101620914019549 1102 20-21 0.09791000785539694 1102 22-23 0.11083124574783199 1102 24-25 0.12181381271772551 1102 26-27 0.16483357060844384 1102 28-29 0.20068379073207865 1102 30-31 0.2572114119528379 1102 32-33 0.2527246574193356 1102 34-35 0.34092118257750315 1102 36-37 0.4255198000572946 1102 38-39 0.4669674366118244 1102 40-41 0.35772287668561376 1102 42-43 0.33997491596454665 1102 44-45 0.25940852323125796 1102 46-47 0.3281014340138455 1102 48-49 0.16075816048731895 1102 50-51 0.19004701440383798 1102 52-53 0.19555612922340515 1102 54-55 0.3034740499324542 1102 56-57 0.22284422949086036 1102 58-59 0.24520526965405054 1102 60-61 0.21381770422572544 1102 62-63 0.09382106782162225 1102 64-65 0.15905212896723953 1102 66-67 0.2582891491007331 1102 68-69 0.21454984019625556 1102 70-71 0.25070042284777116 1102 72-73 0.2951046230045904 1102 74-75 0.28112726730952176 1102 76-77 0.31759991859534153 1102 78-79 0.2642329493667077 1102 80-81 0.34882071900550926 1102 82-83 0.26014683137062633 1102 84-85 0.3362452038571746 1102 86-87 0.195989962753913 1102 88-89 0.27573114636710727 1102 90-91 0.2340593127869326 1102 92-93 0.3538550064915853 1102 94-95 0.2176663911667731 1102 96-97 0.277367962412022 1102 98-99 0.21943267685405843 1102 100-101 0.2316975972475035 1102 102-103 0.23484537949024897 1102 104-105 0.28523397160521213 1102 106-107 0.32541378367567475 1102 108-109 0.3228769855209279 1102 110-111 0.29456174027258797 1102 112-113 0.30882370903114875 1102 114-115 0.3382122302227124 1102 116-117 0.33185272661859955 1102 118-119 0.25384167437018235 1102 120-121 0.20046269533303018 1102 122-123 0.19180047209741602 1102 124-125 0.040195951305712185 1103 1 -0.034132726217304565 1103 2 0.07352035514244903 1103 3 0.19105302719995976 1103 4 0.038063818395713156 1103 5 0.1297806622497788 1103 6 0.17849918900231643 1103 7 0.03713293704683451 1103 8 0.12656429778646583 1103 9 0.1727868258295544 1103 10-11 0.014788047797779313 1103 12-13 0.0793518626387808 1103 14-15 0.1816494196624845 1103 16-17 0.08888454102267929 1103 18-19 0.12696655160595327 1103 20-21 0.02811027587206638 1103 22-23 -0.01804848176855245 1103 24-25 0.019242527726667902 1103 26-27 -0.007264776622044167 1103 28-29 0.0852356072895546 1103 30-31 0.015733039409653315 1103 32-33 0.014410370641485315 1103 34-35 0.005067437788937923 1103 36-37 0.010612303035259174 1103 38-39 0.05666926805911743 1103 40-41 0.052079636661787276 1103 42-43 -0.064765629002693 1103 44-45 -0.03305909142031993 1103 46-47 -0.03719878188847758 1103 48-49 0.018527852268135803 1103 50-51 -0.055262693755786074 1103 52-53 -0.07857002473728869 1103 54-55 0.11795811783001398 1103 56-57 0.034025550217485545 1103 58-59 0.008708470964357673 1103 60-61 -0.044097163254910754 1103 62-63 -0.21521053581150085 1103 64-65 -0.12229000176533589 1103 66-67 -0.20751825113750044 1103 68-69 -0.3567314284160119 1103 70-71 -0.26669646518081436 1103 72-73 -0.3144202057625307 1103 74-75 -0.2953933550847623 1103 76-77 -0.3432612711009 1103 78-79 -0.31364450745282824 1103 80-81 -0.37522819403798024 1103 82-83 -0.3012206120480698 1103 84-85 -0.3295547514733812 1103 86-87 -0.3368951250959924 1103 88-89 -0.33977938817726283 1103 90-91 -0.29811850883307756 1103 92-93 -0.17366974782645528 1103 94-95 -0.3984797523710455 1103 96-97 -0.36630479667076443 1103 98-99 -0.37292698663611645 1103 100-101 -0.3667464170467163 1103 102-103 -0.40390722622744235 1103 104-105 -0.32189452750139935 1103 106-107 -0.2988938399097911 1103 108-109 -0.22618123365715093 1103 110-111 -0.2637160664576079 1103 112-113 -0.2818254869188195 1103 114-115 -0.19478360062898403 1103 116-117 -0.24216082311338383 1103 118-119 -0.2260589361122456 1103 120-121 -0.21781734486947002 1103 122-123 -0.2863952545255728 1103 124-125 -0.43351887150548407 1104 1 -0.18680769256992846 1104 2 -0.015201739479824283 1104 3 0.019701938780507078 1104 4 -0.04268348249959786 1104 5 -0.06806224839449726 1104 6 0.06237734161510389 1104 7 0.1481381889632729 1104 8 0.21492066006629784 1104 9 0.20090707130910346 1104 10-11 0.09618498076635262 1104 12-13 0.0526543159713313 1104 14-15 0.1852075381890188 1104 16-17 0.17999898232995548 1104 18-19 0.12358078444579235 1104 20-21 0.035865630193569586 1104 22-23 0.02214321683874232 1104 24-25 -0.021894588985837515 1104 26-27 -0.0488019130840982 1104 28-29 -0.0723328578049518 1104 30-31 -0.030970523554827878 1104 32-33 0.022365506255042078 1104 34-35 -0.0012611032316058868 1104 36-37 -0.06345169646734661 1104 38-39 -0.07469538903024642 1104 40-41 -0.03351636076345699 1104 42-43 -0.01316719580005099 1104 44-45 -0.0584089144058666 1104 46-47 -0.03998794816503448 1104 48-49 -0.1122027163725079 1104 50-51 -0.044671085118643816 1104 52-53 -0.01599367811080299 1104 54-55 0.05666686322178549 1104 56-57 -0.0803448964148501 1104 58-59 -0.13401217535784582 1104 60-61 -0.005222101080413211 1104 62-63 0.0059308965426509985 1104 64-65 0.07826892868700241 1104 66-67 0.08862894150104239 1104 68-69 0.029260657136099155 1104 70-71 0.10159691798963877 1104 72-73 0.014724730307456468 1104 74-75 0.044777039613585146 1104 76-77 0.040430815182673996 1104 78-79 0.0671980667176939 1104 80-81 0.08189608912663715 1104 82-83 0.10303294652761963 1104 84-85 -0.05078350242428087 1104 86-87 -0.019778211438087112 1104 88-89 -0.0393663849295649 1104 90-91 -0.008566841825057736 1104 92-93 0.14235728345097343 1104 94-95 0.1088650673960565 1104 96-97 0.1359568007018126 1104 98-99 0.040216153688067635 1104 100-101 0.04088056522257588 1104 102-103 -0.03222167558082489 1104 104-105 0.05059837729115557 1104 106-107 0.06764677100951033 1104 108-109 0.009644809411490485 1104 110-111 0.08867298863631135 1104 112-113 -0.025425170926073548 1104 114-115 -0.10788559606464432 1104 116-117 -0.09443970133914448 1104 118-119 0.004135743213634413 1104 120-121 0.061924466383381116 1104 122-123 0.04142435896848795 1104 124-125 -0.04857780927246935 1105 1 0.03218533487167363 1105 2 0.20316391856989924 1105 3 0.21328114371938867 1105 4 0.31098752213149083 1105 5 0.10873080371290911 1105 6 0.13823452302852957 1105 7 0.1317273853406178 1105 8 0.13909118299923762 1105 9 0.31074676144939417 1105 10-11 0.3134741909065042 1105 12-13 0.2676051899094105 1105 14-15 0.3994814430183382 1105 16-17 0.36911912803694236 1105 18-19 0.31407229529785496 1105 20-21 0.24912296424899694 1105 22-23 0.2791743118396255 1105 24-25 0.2902341951150831 1105 26-27 0.23388385349516483 1105 28-29 0.31128431102533227 1105 30-31 0.3551538886215013 1105 32-33 0.4210659043039442 1105 34-35 0.36829342875261517 1105 36-37 0.4564949860712346 1105 38-39 0.356182266788835 1105 40-41 0.3291308520899321 1105 42-43 0.3154733787344668 1105 44-45 0.24958298520073186 1105 46-47 0.33550469767153857 1105 48-49 0.3180900202628294 1105 50-51 0.2406550844069315 1105 52-53 0.21201085825249066 1105 54-55 0.402264731953224 1105 56-57 0.31108623862328955 1105 58-59 0.23343727314692586 1105 60-61 0.19063645661343998 1105 62-63 0.2350811416814338 1105 64-65 0.318567492171951 1105 66-67 0.26267434803104095 1105 68-69 0.17888155443518627 1105 70-71 0.22640991365167906 1105 72-73 0.23522496356017797 1105 74-75 0.12202122572244889 1105 76-77 0.18423156552361064 1105 78-79 0.28115203103092057 1105 80-81 0.1445378504796011 1105 82-83 0.2186288848917073 1105 84-85 0.20163660628399782 1105 86-87 0.27148160349688055 1105 88-89 0.21035657160678767 1105 90-91 0.2488985858317463 1105 92-93 0.23346123909541916 1105 94-95 0.1571591052419805 1105 96-97 0.16330367162994364 1105 98-99 0.054489254197651604 1105 100-101 0.09397951978566255 1105 102-103 0.07641153495969633 1105 104-105 0.11093754998575278 1105 106-107 0.1578552506843529 1105 108-109 0.18753888586476108 1105 110-111 0.13303524351987406 1105 112-113 0.24679192748154932 1105 114-115 0.20938234428700397 1105 116-117 0.329716522576323 1105 118-119 0.15761380047762685 1105 120-121 0.09396767086469282 1105 122-123 0.1440144472470486 1105 124-125 -0.12974528830206467 1106 1 -0.06226177939499422 1106 2 0.08506045693007636 1106 3 0.11054810699468831 1106 4 0.10878207672763551 1106 5 0.062353407978079645 1106 6 0.07487705794721933 1106 7 0.2023527955028257 1106 8 0.049059610198355585 1106 9 0.12410551838016204 1106 10-11 0.1060845536061521 1106 12-13 0.1306730223410284 1106 14-15 0.23154359647971745 1106 16-17 0.14529199816472982 1106 18-19 0.1596148979094565 1106 20-21 0.15270319585177106 1106 22-23 0.21127987144296867 1106 24-25 0.2231019459018171 1106 26-27 0.27346095365483336 1106 28-29 0.20473385913639675 1106 30-31 0.33544284950946945 1106 32-33 0.3199270098170359 1106 34-35 0.22165559616598074 1106 36-37 0.280524419569403 1106 38-39 0.29724034317330705 1106 40-41 0.2852325154753004 1106 42-43 0.2876185206053776 1106 44-45 0.3363642647517864 1106 46-47 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2216 74-75 -0.17296361832884344 2216 76-77 -0.17859490636660524 2216 78-79 -0.10182359940402819 2216 80-81 -0.10014349945024037 2216 82-83 -0.18873225435545038 2216 84-85 -0.1419485633951041 2216 86-87 -0.16512695286149182 2216 88-89 -0.09671551668786904 2216 90-91 -0.10268190951268252 2216 92-93 -0.1204432934335955 2216 94-95 -0.13646770578134237 2216 96-97 -0.21725319694096967 2216 98-99 -0.19264649658453337 2216 100-101 -0.14565516182686977 2216 102-103 -0.13505052639938597 2216 104-105 -0.1979052333066882 2216 106-107 -0.2603232593808471 2216 108-109 -0.11607059335583259 2216 110-111 -0.2181185218330306 2216 112-113 -0.2319503538638763 2216 114-115 -0.14901270076818207 2216 116-117 -0.11319732110732517 2216 118-119 -0.16787426608849998 2216 120-121 -0.20624860152221913 2216 122-123 -0.3209242912077954 2216 124-125 -0.15545131541771084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16581.0 3 3160.0 4 2250.0 5 1620.0 6 2040.0 7 2271.0 8 2376.0 9 2425.0 10 2888.0 11 3583.0 12 4160.0 13 3863.0 14 3854.0 15 3975.0 16 3627.0 17 3787.0 18 2993.0 19 2654.0 20 2401.0 21 2125.0 22 2075.0 23 3048.0 24 6037.0 25 10543.0 26 12139.0 27 7910.0 28 7840.0 29 9355.0 30 10567.0 31 13125.0 32 17238.0 33 24515.0 34 38348.0 35 73261.0 36 187935.0 37 435704.0 38 214768.0 39 876.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.230231202134306 18.788844913195703 13.250435132753621 23.730488751916372 2 15.386408092403814 20.116491092088715 40.05701482193342 24.440085993574048 3 19.240119620643622 25.602372894352527 27.440599218562113 27.716908266441738 4 12.74179257031825 16.421836245600204 35.7074209606469 35.12895022343465 5 14.19281645726793 39.04418677803618 31.860905449478977 14.902091315216909 6 32.40571611828231 35.83946554747223 17.279249005657558 14.475569328587913 7 29.630233206678984 33.394046083591874 19.31502074110224 17.660699968626904 8 26.60910073137573 36.15456994287299 18.83918192655198 18.397147399199298 9 26.92082945225312 18.190753784271248 18.697997885901472 36.19041887757416 10-11 25.224697973240623 28.098609563494364 25.509653133874888 21.167039329390125 12-13 25.490793255751026 25.106149550065332 26.422774323333382 22.980282870850253 14-15 22.968580856648117 26.14743582762489 23.983577099419534 26.90040621630746 16-17 23.250048472094235 29.426199330322618 24.56134388608949 22.762408311493658 18-19 23.359521029692026 27.926613437090474 26.13156430192945 22.582301231288042 20-21 24.38647468651641 27.060490156885812 25.74069936124936 22.812335795348414 22-23 24.22391746553615 26.830328624781707 25.7246947144891 23.221059195193046 24-25 23.7685384530551 27.14871257927459 25.736078424363896 23.34667054330641 26-27 23.31927098852882 27.403667710451618 25.765500380746264 23.5115609202733 28-29 23.376560582690136 27.303690338925392 26.00617590213343 23.313573176251047 30-31 23.519210966045613 27.025641338381686 25.920385700371305 23.534761995201396 32-33 23.250332472440615 27.273150993671152 25.777384381701452 23.699132152186785 34-35 23.715432237515007 27.192072262327304 25.6170403321781 23.475455167979593 36-37 23.889713847884146 26.92852012229624 25.45596468585034 23.72580134396928 38-39 23.45671106836328 27.037292110716304 25.77276071567189 23.73323610524853 40-41 23.562040527054698 26.8166891071616 26.067157700414818 23.55411266536888 42-43 23.430636997696023 26.809975792789416 25.811324897183606 23.948062312330954 44-45 23.225939946245227 27.016479280017077 25.836792835019885 23.920787938717815 46-47 23.580305483782855 27.210544727570866 25.67354159832468 23.5356081903216 48-49 23.570164895269222 27.072299764273524 25.66970324427772 23.68783209617954 50-51 23.50327475011925 27.043707622642494 25.819232050949754 23.6337855762885 52-53 23.765658114557173 26.848860940913553 25.818948523982428 23.566532420546842 54-55 23.546152740325763 26.80993060426132 25.927520006273063 23.716396649139853 56-57 23.473916611921496 27.23395153266152 25.701551393954393 23.590580461462594 58-59 23.518423159796917 26.694434932775007 25.804534757382726 23.98260715004535 60-61 23.558072420364827 26.93789273073782 25.98451402123605 23.51952082766131 62-63 23.25008255663298 27.121661902052548 25.947658659036314 23.680596882278163 64-65 23.206126360543255 26.930009659579348 25.911984980378026 23.951878999499375 66-67 23.265330307878383 27.30033042987253 25.665608803097957 23.76873045915113 68-69 23.29854395265635 26.912453721031664 26.063060091527458 23.72594223478453 70-71 23.25512294457016 26.88664736313327 26.062340595539858 23.79588909675671 72-73 23.27536296282743 27.06079722655319 25.762334114432562 23.901505696186813 74-75 23.249597817739232 27.24916045950494 25.68582257979501 23.81541914296082 76-77 23.508028015504536 26.97908072274356 25.742533714702915 23.770357547048988 78-79 23.38886020690241 27.111143060665672 25.86855227128657 23.63144446114535 80-81 23.265819840001253 27.434517749248165 25.89713395483911 23.402528455911472 82-83 23.5087074208146 27.216609645364244 25.696007695588584 23.578675238232577 84-85 23.430682182538025 27.098537284095187 25.732098545996962 23.73868198736983 86-87 23.42608381962495 27.06048372691222 25.921398080044895 23.592034373417935 88-89 23.36824393210863 27.01899968455853 25.895784063757514 23.716972319575323 90-91 23.31459438075408 27.351159083294448 25.852609271707845 23.48163726424363 92-93 23.38073778045404 26.951561017884835 25.8608331848268 23.806868016834322 94-95 23.15456519892212 26.874908778801395 26.009205988903144 23.961320033373344 96-97 23.448537875639044 27.0236168876039 25.82334083587557 23.704504400881483 98-99 23.209033846861708 26.96651390657147 25.790836311414484 24.033615935152337 100-101 22.96101766466714 27.062094047459823 26.08064135981385 23.89624692805919 102-103 23.24705546098543 26.784728524678446 26.07219212776683 23.896023886569296 104-105 22.94634435375128 27.122383494860113 26.109263119830434 23.82200903155817 106-107 23.048923687587276 27.11902069839942 26.019463161816127 23.812592452197176 108-109 23.192955062514102 27.067095597044773 25.734559247025036 24.005390093416086 110-111 22.907004960879757 27.344655019105947 26.03418463081374 23.714155389200556 112-113 23.00114410793129 27.397463792425885 26.03207161958803 23.56932048005479 114-115 22.83482883810649 27.425065757776146 25.84910082394341 23.891004580173956 116-117 22.776060223638765 27.389671529205945 26.115394952990034 23.718873294165256 118-119 22.740929996671237 27.560227052895936 25.756813939667506 23.94202901076532 120-121 22.69525298684234 27.46971333371382 26.05435327669337 23.78068040275047 122-123 22.790225354603827 27.471749535711748 25.961631036019533 23.776394073664893 124-125 22.985057070663064 27.528535331532634 25.705149429293368 23.781258168510934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1127.0 1 1033.0 2 896.5 3 777.0 4 599.5 5 431.0 6 362.0 7 356.5 8 385.5 9 370.5 10 308.0 11 304.5 12 306.0 13 313.5 14 355.0 15 373.5 16 392.0 17 459.0 18 571.5 19 698.5 20 919.5 21 1204.5 22 1494.5 23 1952.5 24 2495.0 25 3132.0 26 3991.5 27 4875.0 28 5816.0 29 7388.0 30 9707.5 31 12042.0 32 14378.0 33 16538.0 34 18850.0 35 20642.5 36 22985.5 37 26037.5 38 28001.5 39 28635.0 40 28811.0 41 30799.0 42 32973.5 43 34980.5 44 37812.5 45 40314.0 46 40702.5 47 41351.0 48 43577.0 49 44523.0 50 43883.5 51 42717.5 52 42843.0 53 44833.0 54 45200.5 55 43302.0 56 39959.0 57 36075.0 58 33797.5 59 30840.0 60 26938.0 61 22683.0 62 18297.5 63 14142.5 64 10757.0 65 8584.0 66 6605.5 67 5289.0 68 3977.0 69 2548.5 70 1843.5 71 1397.0 72 1106.0 73 957.5 74 744.5 75 564.5 76 404.0 77 249.0 78 177.0 79 117.5 80 63.0 81 38.5 82 32.5 83 24.0 84 18.0 85 16.0 86 12.5 87 8.5 88 11.5 89 13.0 90 6.5 91 4.5 92 7.0 93 6.0 94 4.5 95 2.5 96 4.0 97 5.0 98 2.5 99 4.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04930670074578563 2 0.19600720261133864 3 0.025176036246523047 4 0.1108965195218818 5 0.02317240706427381 6 0.037023582715475074 7 0.038068954462735546 8 0.1022722026069829 9 0.11577492100909735 10-11 0.04734662871967224 12-13 0.031491823886221744 14-15 0.011281303439185935 16-17 0.030446452138961265 18-19 0.05422865938913702 20-21 0.1953974024254367 22-23 0.10841376162213819 24-25 0.07626858039387865 26-27 0.014896547398461735 28-29 0.005967330390611865 30-31 0.007230487918551602 32-33 0.00675135920105722 34-35 0.0086243169148989 36-37 0.004225044145177744 38-39 0.008754988383306458 40-41 0.005793101766068453 42-43 0.010323046004197168 44-45 0.00975680297443108 46-47 0.017117962361390238 48-49 0.01642104786321659 50-51 0.010410160316468874 52-53 0.013023589684620055 54-55 0.013241375465299321 56-57 0.01524500464754856 58-59 0.017771319703428035 60-61 0.009103445632393284 62-63 0.019165148699775335 64-65 0.03062068076350468 66-67 0.027963694239217643 68-69 0.03419236756664463 70-71 0.02491469330970793 72-73 0.015288561803684413 74-75 0.010148817379653756 76-77 0.010802174721691551 78-79 0.02391287871858331 80-81 0.019208705855911185 82-83 0.02147367797497554 84-85 0.016464605019352443 86-87 0.02491469330970793 88-89 0.027789465614674227 90-91 0.026787651023549613 92-93 0.03314699581938415 94-95 0.025655164964017433 96-97 0.02665697955514205 98-99 0.03188383829144442 100-101 0.020079848978628245 102-103 0.02844282295671203 104-105 0.02391287871858331 106-107 0.03175316682303686 108-109 0.02230126394155675 110-111 0.020689649164530186 112-113 0.025960065056968403 114-115 0.012544460967125673 116-117 0.011194189126914228 118-119 0.02988020910919518 120-121 0.022519049722236017 122-123 0.016769505112303414 124-125 0.018903805762960215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1147917.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.77359237935629 #Duplication Level Percentage of deduplicated Percentage of total 1 71.77651228465413 33.57245328014272 2 13.537050357136648 12.66352950847058 3 5.412259664486829 7.5945248219381565 4 2.740356913177134 5.127053489235934 5 1.6046340669300103 3.7527249882306513 6 1.0049661972630664 2.820352755948866 7 0.6855168220173794 2.244485908156285 8 0.4993151730329198 1.8683811497815634 9 0.3665585539582783 1.543073434740971 >10 2.1560371949990618 18.567187988377295 >50 0.1501384331462971 4.759321132927509 >100 0.06460630177184425 4.80790577266342 >500 0.001861852205816179 0.5495966923939434 >1k 1.861852205816179E-4 0.12940907699214066 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1486 0.1294518680357552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.355715613585303E-5 0.0 18-19 0.0 0.0 0.0 8.711431227170606E-5 0.0 20-21 0.0 0.0 0.0 8.711431227170606E-5 0.0 22-23 0.0 0.0 0.0 3.0490009295097123E-4 0.0 24-25 0.0 0.0 0.0 4.355715613585303E-4 0.0 26-27 0.0 0.0 0.0 6.969144981736485E-4 0.0 28-29 0.0 0.0 0.0 0.0020036291822492394 0.0 30-31 0.0 0.0 0.0 0.004268601301313597 0.0 32-33 0.0 0.0 0.0 0.010279488848061315 0.0 34-35 0.0 0.0 0.0 0.017335748142069507 0.0 36-37 0.0 0.0 0.0 0.03105625232486321 0.0 38-39 0.0 0.0 0.0 0.05353174489096337 0.0 40-41 0.0 0.0 0.0 0.07923046701111666 0.0 42-43 0.0 0.0 0.0 0.10501630344354165 0.0 44-45 0.0 0.0 0.0 0.13393825511774807 0.0 46-47 0.0 0.0 0.0 0.17000358039823438 0.0 48-49 0.0 0.0 0.0 0.21268959341137034 0.0 50-51 0.0 0.0 0.0 0.2659599953655186 0.0 52-53 0.0 0.0 0.0 0.312958166836104 0.0 54-55 0.0 0.0 0.0 0.3628746677677916 0.0 56-57 0.0 0.0 0.0 0.4233320004843556 0.0 58-59 0.0 0.0 0.0 0.49642090848031695 0.0 60-61 0.0 0.0 0.0 0.5778727904543621 0.0 62-63 0.0 0.0 0.0 0.6577566148075166 0.0 64-65 0.0 0.0 0.0 0.7362901673204596 0.0 66-67 0.0 0.0 0.0 0.8280215381425661 0.0 68-69 0.0 0.0 0.0 0.9387002718837686 0.0 70-71 0.0 0.0 0.0 1.0638399814620745 0.0 72-73 0.0 0.0 0.0 1.1928127207803352 0.0 74-75 0.0 0.0 0.0 1.3181266589831844 0.0 76-77 0.0 0.0 0.0 1.4546347863129476 0.0 78-79 0.0 0.0 0.0 1.6204133225660042 0.0 80-81 0.0 0.0 0.0 1.8082753369799385 0.0 82-83 0.0 0.0 0.0 2.0145620284393386 0.0 84-85 0.0 0.0 0.0 2.21431514647836 0.0 86-87 0.0 0.0 0.0 2.4259158109863344 0.0 88-89 0.0 0.0 0.0 2.6573349815361214 0.0 90-91 0.0 0.0 0.0 2.923382091213912 0.0 92-93 0.0 0.0 0.0 3.2127322794243836 0.0 94-95 0.0 0.0 0.0 3.499730381203519 0.0 96-97 0.0 0.0 0.0 3.7952656855852815 0.0 98-99 0.0 0.0 0.0 4.107744723703892 0.0 100-101 0.0 0.0 0.0 4.489436082922372 0.0 102-103 0.0 0.0 0.0 4.895040320859435 0.0 104-105 0.0 0.0 0.0 5.302909530915563 0.0 106-107 0.0 0.0 0.0 5.702241538369063 0.0 108-109 0.0 0.0 0.0 6.1230907809536745 0.0 110-111 0.0 0.0 0.0 6.597341096960843 0.0 112-113 0.0 0.0 0.0 7.107438952467818 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1510 0.0 56.48752 1 GTATCAA 2605 0.0 55.182674 1 TATCACG 65 3.4717108E-5 45.876797 2 ATCAACG 3435 0.0 41.28463 3 TCAACGC 3435 0.0 41.114754 4 CAACGCA 3550 0.0 39.77245 5 AACGCAG 3645 0.0 38.8993 6 TATCAAC 3805 0.0 38.24473 2 GTACATG 7930 0.0 35.72842 1 TACATGG 7850 0.0 34.644287 2 ACATGGG 8160 0.0 31.76415 3 CGCAGAG 4510 0.0 31.042282 8 ACGCAGA 4530 0.0 31.036743 7 GCAGAGT 5045 0.0 27.384174 9 CATGGGA 4910 0.0 27.064466 4 TAACGCC 90 7.3128285E-7 26.437286 64-65 CATGGGG 3590 0.0 26.226402 4 GTTCGCG 205 7.675408E-8 26.154776 7 TCGCGCA 185 9.1929724E-7 25.750822 9 ATGGGAT 2100 0.0 23.546217 5 >>END_MODULE