FastQCFastQC Report
Fri 27 May 2016
SRR940004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147917
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71630.6239998188022304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.435658675670802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.285996287188011No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.123876552050366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.067.703011
GTATCAA58500.061.7592471
TAATGCG350.00778303651.021895
ATCAACG76600.047.0799523
TCAACGC77850.046.400584
TATCAAC78550.045.9152032
CAACGCA79250.045.592435
AACGCAG82350.043.929286
TATAACG1501.8189894E-1243.7168352
GTGGTAT12550.040.8545041
TATCACG1053.2151365E-739.742582
ACGCAGA96650.037.1801727
CGCAGAG96800.037.0610968
TAACGCA1703.2014214E-1035.0639234
TGGTATC13600.034.1903082
GCAGAGT105100.033.9078649
GTACATG76850.032.3502351
GTCGCGC750.00391227931.730368
TACATGG79000.031.693452
ACATGGG77700.031.3003033
Produced by FastQC (version 0.11.5)