##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940003_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88899 Sequences flagged as poor quality 0 Sequence length 125 %GC 26 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.78766915263389 26.0 10.0 34.0 7.0 34.0 2 22.725441231059968 26.0 12.0 34.0 7.0 34.0 3 24.11639051057942 28.0 16.0 34.0 7.0 34.0 4 24.89448700210351 31.0 12.0 37.0 7.0 37.0 5 28.04764957986029 32.0 26.0 37.0 7.0 37.0 6 25.608870740953215 31.0 13.0 37.0 7.0 37.0 7 26.75496912226234 32.0 17.0 37.0 7.0 37.0 8 25.342658522593055 32.0 12.0 37.0 2.0 37.0 9 25.72980573459769 32.0 12.0 39.0 2.0 39.0 10-11 26.920347810436564 32.0 12.5 39.0 2.0 39.0 12-13 26.97932485179811 32.0 13.5 39.0 2.0 39.0 14-15 27.74339981327124 32.0 12.5 41.0 2.0 41.0 16-17 28.035939661863463 32.0 13.0 41.0 2.0 41.0 18-19 27.556440454898258 32.0 13.0 41.0 2.0 41.0 20-21 27.219276932248956 31.5 13.0 41.0 2.0 41.0 22-23 27.180806308282435 31.0 17.5 41.0 2.0 41.0 24-25 27.18087942496541 30.0 20.5 41.0 2.0 41.0 26-27 27.04960685721999 30.0 23.5 41.0 2.0 41.0 28-29 26.844857647442602 30.0 23.0 41.0 2.0 41.0 30-31 26.628820346685565 30.0 22.0 41.0 2.0 41.0 32-33 26.452035455966886 30.0 18.0 40.0 2.0 41.0 34-35 26.141385167437207 30.0 2.0 40.0 2.0 41.0 36-37 25.688039235536955 30.0 2.0 40.0 2.0 41.0 38-39 25.323935027390633 30.0 2.0 40.0 2.0 41.0 40-41 25.067717297157447 30.0 2.0 40.0 2.0 41.0 42-43 24.70450736228754 29.0 2.0 40.0 2.0 41.0 44-45 24.531687645530322 29.0 2.0 40.0 2.0 41.0 46-47 24.31317562627251 29.0 2.0 40.0 2.0 41.0 48-49 24.129860853327934 28.5 2.0 39.5 2.0 41.0 50-51 24.001833541434664 29.0 2.0 39.0 2.0 41.0 52-53 23.982395752483157 29.0 2.0 39.0 2.0 41.0 54-55 23.92071339385145 29.5 2.0 39.0 2.0 41.0 56-57 23.912507452277303 30.0 2.0 39.0 2.0 41.0 58-59 23.721464808378048 30.0 2.0 38.5 2.0 41.0 60-61 23.483936827185907 30.0 2.0 38.0 2.0 41.0 62-63 23.214271251645126 30.0 2.0 37.0 2.0 41.0 64-65 22.979476709524292 29.0 2.0 36.5 2.0 41.0 66-67 22.74813552458408 29.0 2.0 36.0 2.0 40.0 68-69 22.51176053723889 28.5 2.0 35.5 2.0 40.0 70-71 22.249997187819886 28.0 2.0 35.0 2.0 39.0 72-73 21.984825476102095 28.0 2.0 35.0 2.0 39.0 74-75 21.748782326010417 28.0 2.0 35.0 2.0 38.0 76-77 21.485039201790798 28.0 2.0 35.0 2.0 37.0 78-79 21.275897366674542 28.0 2.0 35.0 2.0 37.0 80-81 21.112447834058877 28.0 2.0 35.0 2.0 36.0 82-83 20.924875420420925 28.0 2.0 35.0 2.0 36.0 84-85 20.741886860369632 28.0 2.0 35.0 2.0 35.5 86-87 20.555759907310545 28.0 2.0 35.0 2.0 35.0 88-89 20.401629939594372 28.0 2.0 35.0 2.0 35.0 90-91 20.257888165221207 28.0 2.0 35.0 2.0 35.0 92-93 20.121525551468523 28.0 2.0 35.0 2.0 35.0 94-95 20.048431365932124 28.0 2.0 35.0 2.0 35.0 96-97 19.952704754834137 28.0 2.0 35.0 2.0 35.0 98-99 19.858772314649208 27.0 2.0 35.0 2.0 35.0 100-101 19.729023948525857 27.0 2.0 35.0 2.0 35.0 102-103 19.305880831055468 25.5 2.0 33.5 2.0 35.0 104-105 19.747696824486212 27.0 2.0 35.0 2.0 35.0 106-107 19.77031237696712 27.0 2.0 35.0 2.0 35.0 108-109 19.74932788895263 27.0 2.0 35.0 2.0 35.0 110-111 19.688877265211083 26.5 2.0 35.0 2.0 35.0 112-113 19.608904487114593 25.5 2.0 35.0 2.0 35.0 114-115 19.4765520422052 25.0 2.0 35.0 2.0 35.0 116-117 19.360409003475855 25.0 2.0 35.0 2.0 35.0 118-119 19.266105355515812 25.0 2.0 35.0 2.0 35.0 120-121 19.118089067368587 25.0 2.0 35.0 2.0 35.0 122-123 18.914909054095098 25.0 2.0 35.0 2.0 35.0 124-125 18.241031957614823 25.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11313480340990978 1101 2 0.07777709409768363 1101 3 -0.3762050800600427 1101 4 -0.25488395637409766 1101 5 0.4209942205859676 1101 6 -0.145842910047417 1101 7 -0.31063601794992834 1101 8 0.07162431733815211 1101 9 0.5095258892910195 1101 10-11 0.9428206510043502 1101 12-13 0.6612801740984331 1101 14-15 0.7077806837990614 1101 16-17 0.6250784039839026 1101 18-19 0.06078049840302313 1101 20-21 -0.4291859034867542 1101 22-23 -0.9688949247227967 1101 24-25 -1.7551237977697198 1101 26-27 -1.7511018500150755 1101 28-29 -1.5997353618314243 1101 30-31 -1.1753311041252985 1101 32-33 -1.2388137759257134 1101 34-35 -1.1122781745238228 1101 36-37 -0.7991991451361216 1101 38-39 -0.4517704518039629 1101 40-41 -0.3884962020753733 1101 42-43 0.09727645305735066 1101 44-45 0.021464391633546853 1101 46-47 -0.43612486611336365 1101 48-49 -0.5245139715754803 1101 50-51 -0.5386608108793425 1101 52-53 -0.5997326905064853 1101 54-55 -0.5444448708078262 1101 56-57 -0.5389354475527028 1101 58-59 -0.2983082432134978 1101 60-61 -0.08918603097645317 1101 62-63 -0.17632598955883338 1101 64-65 -0.22706877811820192 1101 66-67 -0.33465396615778786 1101 68-69 -0.2248860280260203 1101 70-71 -0.18462585946937438 1101 72-73 -0.2436652664737089 1101 74-75 -0.14027640403663355 1101 76-77 0.052928928500296735 1101 78-79 -0.16876083266611275 1101 80-81 -0.21255153865736176 1101 82-83 -0.019313361908881888 1101 84-85 0.16233771986097523 1101 86-87 0.3856809667906944 1101 88-89 0.5029449890979514 1101 90-91 0.27261715735128433 1101 92-93 0.47606791603278253 1101 94-95 0.42396774373183277 1101 96-97 0.4454090776852162 1101 98-99 0.3123306738556373 1101 100-101 -0.0602056118645784 1101 102-103 -0.14425953974214778 1101 104-105 -0.27484821017538863 1101 106-107 -0.3518476459879736 1101 108-109 -0.3042834302667998 1101 110-111 -0.1498375593162926 1101 112-113 5.225817839438207E-4 1101 114-115 0.1304593803691141 1101 116-117 0.19384939237736631 1101 118-119 0.1450269637668491 1101 120-121 -0.19699405715873297 1101 122-123 0.028442766825641286 1101 124-125 0.0023990411559999814 1102 1 -0.01121172648683455 1102 2 0.19823163955222967 1102 3 -0.7380931919481526 1102 4 -0.0061077325978722286 1102 5 0.47921100380275305 1102 6 -0.5411226303271377 1102 7 0.06663670932280041 1102 8 -0.39725680154296583 1102 9 -0.5013132715481419 1102 10-11 -0.6593646636809645 1102 12-13 -0.3637198259015655 1102 14-15 0.18671425023262955 1102 16-17 0.284606376011876 1102 18-19 0.22791336553589048 1102 20-21 -0.7138012881021396 1102 22-23 -0.7122515680794379 1102 24-25 -0.7623790425249624 1102 26-27 -0.6316962556094801 1102 28-29 -0.4724626345586991 1102 30-31 -0.38057585937005456 1102 32-33 -0.47666342627536196 1102 34-35 -1.1872781745238257 1102 36-37 -0.7736746696116441 1102 38-39 -0.9574522699857795 1102 40-41 -1.0403318664110373 1102 42-43 -1.007968302187404 1102 44-45 -0.9564202237510671 1102 46-47 -0.8825409500294477 1102 48-49 -0.829059426120935 1102 50-51 -0.9429440276625556 1102 52-53 -0.837232690506486 1102 54-55 -1.0506511645141217 1102 56-57 -1.19470467832193 1102 58-59 -1.0781159355211898 1102 60-61 -1.099762954053375 1102 62-63 -1.3331441713770147 1102 64-65 -1.4505827641321893 1102 66-67 -1.2431329871368106 1102 68-69 -1.5245363776763732 1102 70-71 -1.6422307545742711 1102 72-73 -1.222511420319865 1102 74-75 -0.8569722082324382 1102 76-77 -0.9695361064647372 1102 78-79 -0.851016077421356 1102 80-81 -0.9848417484475718 1102 82-83 -0.7953623129578347 1102 84-85 -0.6308440983208428 1102 86-87 -0.35785050174077426 1102 88-89 -0.58831375216079 1102 90-91 -0.9539562692221395 1102 92-93 -0.6779530629881982 1102 94-95 -1.7116965919325047 1102 96-97 -1.3560769363007985 1102 98-99 -1.2279665289415682 1102 100-101 -0.894558758717725 1102 102-103 -0.9120917075743158 1102 104-105 -0.8790440143711926 1102 106-107 -0.7679315620718867 1102 108-109 -0.8475526610360298 1102 110-111 -0.8320928040715394 1102 112-113 -0.6332186769573127 1102 114-115 -0.7575650951553641 1102 116-117 -0.46576599223801907 1102 118-119 -0.8485919173520315 1102 120-121 -1.202938113102789 1102 122-123 -0.9054733170904434 1102 124-125 -0.4614995602426042 1103 1 0.1825173705031311 1103 2 0.17540555259570922 1103 3 0.7747830622324479 1103 4 0.15183541121483657 1103 5 0.7466859201907106 1103 6 0.12016499509092426 1103 7 -0.3181458993728512 1103 8 -0.588059477128251 1103 9 0.33798438731473723 1103 10-11 0.5995400185932773 1103 12-13 0.22867147844626246 1103 14-15 0.3091640830085467 1103 16-17 -0.4567397778342759 1103 18-19 -0.5332906478420334 1103 20-21 -0.10527286000849045 1103 22-23 0.04276515432858474 1103 24-25 0.3800540678429307 1103 26-27 0.11688233970824768 1103 28-29 0.13840693065869303 1103 30-31 0.21775189982727028 1103 32-33 0.30782654027982304 1103 34-35 -0.4057564353933927 1103 36-37 -0.2626378803139886 1103 38-39 0.16048251262291657 1103 40-41 0.34114806669933273 1103 42-43 0.3251420657055739 1103 44-45 -0.17201386923601802 1103 46-47 -0.12940549852443084 1103 48-49 0.16007100866167434 1103 50-51 0.3870308887254055 1103 52-53 0.2915716573196008 1103 54-55 0.4682033505360508 1103 56-57 0.7132384654907789 1103 58-59 0.7843004524386785 1103 60-61 0.8064661429365927 1103 62-63 0.5744645242751574 1103 64-65 0.5160142258343683 1103 66-67 0.607243267043792 1103 68-69 0.7828214818554038 1103 70-71 0.8515401484357632 1103 72-73 0.8737260378741141 1103 74-75 0.7694073904297696 1103 76-77 0.8343518534014827 1103 78-79 0.9359822503378403 1103 80-81 1.0281599237932326 1103 82-83 0.8075641084468508 1103 84-85 0.6528515538530684 1103 86-87 0.6967481604665835 1103 88-89 0.8412849100465678 1103 90-91 0.8781507541892282 1103 92-93 1.2167793784833734 1103 94-95 0.8508452140875598 1103 96-97 0.9436304215587334 1103 98-99 0.9071923339346881 1103 100-101 1.0569880640247504 1103 102-103 0.9648313693487616 1103 104-105 1.071792106030145 1103 106-107 0.8196938559883122 1103 108-109 0.5565861349505923 1103 110-111 0.5070794367311358 1103 112-113 0.7143565738788062 1103 114-115 0.37433289815567505 1103 116-117 0.7351537402034545 1103 118-119 0.6873194538854257 1103 120-121 0.8958913183353374 1103 122-123 0.7873360474580515 1103 124-125 0.9259168277172627 1104 1 -0.21755958217097415 1104 2 0.37632898468497444 1104 3 -0.6167521436159547 1104 4 -0.1481261124481108 1104 5 0.2658856123800142 1104 6 0.292692890435287 1104 7 0.32548626684492277 1104 8 0.18562270832769556 1104 9 -0.9014153818272419 1104 10-11 -0.1075011510873658 1104 12-13 -0.08164017988386618 1104 14-15 -0.2648259131438202 1104 16-17 -1.3045273884537458 1104 18-19 -1.0736523254103325 1104 20-21 -1.1721062574690535 1104 22-23 -0.4520405401692962 1104 24-25 -0.262847047970844 1104 26-27 -0.8724212385911017 1104 28-29 -0.8463564398684333 1104 30-31 -1.0078733406498372 1104 32-33 -1.2820157067382638 1104 34-35 -1.035729501957455 1104 36-37 -0.6273166028995973 1104 38-39 -1.1770319160034788 1104 40-41 -1.4535806751244493 1104 42-43 -1.4494250755025853 1104 44-45 -1.599332068543447 1104 46-47 -1.6753847213024216 1104 48-49 -1.3887939393952706 1104 50-51 -1.6380172066959133 1104 52-53 -1.7669893276746258 1104 54-55 -1.5172525940580286 1104 56-57 -1.515926597995179 1104 58-59 -1.4239719600276572 1104 60-61 -1.2679470929233538 1104 62-63 -1.2214185076602 1104 64-65 -1.166248182784333 1104 66-67 -1.3480087529638993 1104 68-69 -1.1732770175674538 1104 70-71 -1.1598873236688938 1104 72-73 -1.1958776558542432 1104 74-75 -1.0538323171500679 1104 76-77 -0.8460252147016334 1104 78-79 -0.9366610740176782 1104 80-81 -1.0275153359220468 1104 82-83 -1.0736979154084807 1104 84-85 -1.087702505400486 1104 86-87 -1.192516941495711 1104 88-89 -1.171873997225461 1104 90-91 -1.2779862215706785 1104 92-93 -1.107922429904466 1104 94-95 -1.6242221195022815 1104 96-97 -1.499924791984938 1104 98-99 -1.1801874275120205 1104 100-101 -1.242627172604724 1104 102-103 -1.2997704005627426 1104 104-105 -1.368492618864046 1104 106-107 -1.297543543011301 1104 108-109 -1.1007436072579502 1104 110-111 -1.2363218714643232 1104 112-113 -1.00872922835282 1104 114-115 -0.8569098875311276 1104 116-117 -1.159690430631482 1104 118-119 -1.475166117488179 1104 120-121 -1.0349667039809347 1104 122-123 -1.1568347874784628 1104 124-125 -1.1071343458110157 1105 1 -0.4698915601666691 1105 2 -1.1812728649522732 1105 3 -0.6800543839093471 1105 4 -1.1706087720989125 1105 5 -1.2862948867031392 1105 6 -1.1591926233971286 1105 7 -0.4941741014880101 1105 8 -1.3281299824161472 1105 9 -1.2755682958031649 1105 10-11 -1.7814627232790272 1105 12-13 -1.3445756817574228 1105 14-15 -1.188329062310686 1105 16-17 -0.7053884264829264 1105 18-19 -0.7776306400081161 1105 20-21 -1.511618335919188 1105 22-23 -1.9917040975319686 1105 24-25 -1.550537393183312 1105 26-27 -1.424402423315648 1105 28-29 -0.9206608327568979 1105 30-31 -0.8317193105135061 1105 32-33 -0.7309513680633017 1105 34-35 -1.1974133096589625 1105 36-37 -1.523154919091894 1105 38-39 -1.6798621798956894 1105 40-41 -1.547218560797731 1105 42-43 -1.5446973389164391 1105 44-45 -1.3528599326907766 1105 46-47 -1.3896875196760163 1105 48-49 -1.0520323990939104 1105 50-51 -1.2945985668170934 1105 52-53 -1.6204534112272064 1105 54-55 -1.7048871312500857 1105 56-57 -2.077674186291439 1105 58-59 -2.0586686035738566 1105 60-61 -2.3585554003458213 1105 62-63 -2.235171198404039 1105 64-65 -2.0757993268487525 1105 66-67 -2.080927512431334 1105 68-69 -1.7494151025550941 1105 70-71 -1.9194743443178588 1105 72-73 -1.8130346358430813 1105 74-75 -1.6409725547327838 1105 76-77 -2.267136591565226 1105 78-79 -2.0389492028544822 1105 80-81 -1.589701415807241 1105 82-83 -1.3560864986820214 1105 84-85 -2.0486368911136346 1105 86-87 -2.212926731817003 1105 88-89 -2.104426018273056 1105 90-91 -2.0433533586192283 1105 92-93 -2.02540628544142 1105 94-95 -1.9407333209692332 1105 96-97 -1.9189234366472974 1105 98-99 -2.306752045227082 1105 100-101 -2.1386249402839077 1105 102-103 -1.911745207227817 1105 104-105 -1.802120937448116 1105 106-107 -1.9280147221550479 1105 108-109 -1.8403194663028373 1105 110-111 -1.7857916952704294 1105 112-113 -1.8704847892234255 1105 114-115 -1.7090983591886264 1105 116-117 -1.411105562577589 1105 118-119 -1.589452970713083 1105 120-121 -1.9993691595338348 1105 122-123 -1.9233180849352074 1105 124-125 -1.817052228295271 1106 1 2.485141058659053 1106 2 2.469783363690162 1106 3 1.956867020253437 1106 4 1.892764946447219 1106 5 1.9808688287364404 1106 6 2.3895802874447476 1106 7 1.6787056748400424 1106 8 2.336828079093639 1106 9 2.476140309353717 1106 10-11 2.5085730021015245 1106 12-13 1.847487070650157 1106 14-15 1.9852885207896556 1106 16-17 1.9820533256140003 1106 18-19 1.2471441347666605 1106 20-21 1.5501244413408344 1106 22-23 1.0898041348383316 1106 24-25 1.678105042355675 1106 26-27 2.210810375690258 1106 28-29 1.915468399924059 1106 30-31 1.8793710902320697 1106 32-33 1.8666721174912162 1106 34-35 2.084273549614103 1106 36-37 2.0184340837040047 1106 38-39 2.294702902428014 1106 40-41 2.234701290087635 1106 42-43 1.9588751991388555 1106 44-45 2.247326460599062 1106 46-47 1.8994550711907117 1106 48-49 2.0028371256032003 1106 50-51 1.500524142098719 1106 52-53 1.363198343976272 1106 54-55 1.491291806308162 1106 56-57 1.6050300696886808 1106 58-59 1.8016917567865036 1106 60-61 1.9202967276442386 1106 62-63 2.0677178975885013 1106 64-65 1.490564450721923 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70-71 -2.3434670183105375 2216 72-73 -2.2348740576825037 2216 74-75 -2.069197482957712 2216 76-77 -1.8809372053658393 2216 78-79 -2.017911681816962 2216 80-81 -2.2465175726233966 2216 82-83 -2.123659016254539 2216 84-85 -2.20364629612304 2216 86-87 -2.2603230292133034 2216 88-89 -2.0004016642487024 2216 90-91 -2.1407694560353256 2216 92-93 -2.030975041010173 2216 94-95 -1.8270812073171214 2216 96-97 -1.7729725406964008 2216 98-99 -1.9140929025679405 2216 100-101 -2.185080736739703 2216 102-103 -2.2335202790028887 2216 104-105 -1.9559670912942693 2216 106-107 -2.1745249686652954 2216 108-109 -2.1064812324646027 2216 110-111 -1.9028345623132985 2216 112-113 -1.9367901055287398 2216 114-115 -1.9697903698806343 2216 116-117 -1.810546212018238 2216 118-119 -1.6597182909784038 2216 120-121 -1.430960091124767 2216 122-123 -1.5730557346728595 2216 124-125 -1.7643841756272174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9095.0 3 2945.0 4 3844.0 5 2743.0 6 3340.0 7 2323.0 8 1606.0 9 1140.0 10 1025.0 11 1004.0 12 838.0 13 690.0 14 624.0 15 535.0 16 502.0 17 462.0 18 350.0 19 332.0 20 300.0 21 295.0 22 291.0 23 1228.0 24 4576.0 25 1522.0 26 4597.0 27 2408.0 28 1469.0 29 1084.0 30 699.0 31 978.0 32 1398.0 33 1704.0 34 2385.0 35 6776.0 36 5666.0 37 11512.0 38 6570.0 39 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57128869982106 38.14555971954939 7.883453188831494 12.399698391798058 2 9.546894553489892 50.3455506826458 22.86609770121422 17.24145706265009 3 13.1703310304476 59.937664558813594 14.237009699124602 12.654994711614195 4 7.1635302994470536 42.4777864117031 18.261878216606416 32.096805072243434 5 7.241631504922644 66.39887482419128 15.29901547116737 11.060478199718705 6 15.317616609463794 58.74641309852022 9.94654813481123 15.98942215720475 7 15.312243267272132 64.72872142881259 9.20917879289195 10.74985651102333 8 14.508028736797062 62.128684998761344 9.776366461725559 13.586919802716032 9 15.188034284298377 54.23203847410094 8.681451113338664 21.898476128262022 10-11 12.584410030162518 61.098793499302204 11.302503038761087 15.014293431774187 12-13 14.67192546164493 60.45101106147389 11.765897352222984 13.111166124658197 14-15 14.873545890241433 58.27464392592759 12.338838512251648 14.512971671579326 16-17 13.404938868977624 59.55450028976048 13.436446805039019 13.604114036222873 18-19 13.87367816803516 57.408168203996865 15.900252688686411 12.817900939281554 20-21 16.873288327378873 54.775214416932464 15.518038070980175 12.833459184708495 22-23 16.2134009009009 55.55067567567568 14.89132882882883 13.344594594594595 24-25 15.410854807659833 55.61484683711034 15.60167516633456 13.372623188895268 26-27 15.439352432596626 54.91947596091645 15.925928009315252 13.715243597171675 28-29 14.931686381712536 55.29156331032776 16.795194141172104 12.9815561667876 30-31 14.84208454031555 56.41420817279298 16.373147340889275 12.370559946002194 32-33 14.209940152094678 56.46458623948162 16.20224542141025 13.123228187013455 34-35 14.078232402209498 56.616116729854085 16.328792089009887 12.976858778926525 36-37 14.516002024860791 57.30131053490073 15.803476011024243 12.37921142921424 38-39 14.537866174683886 57.69473068442604 15.663389281375151 12.104013859514918 40-41 13.258975527169847 58.1920141291081 16.35253024652819 12.196480097193865 42-43 13.099557310563235 58.810194794602225 15.97281988108698 12.11742801374756 44-45 13.178307655095992 59.05375836019282 15.638130916822762 12.129803067888421 46-47 12.552880288028803 59.305243024302435 15.536678667866788 12.60519801980198 48-49 12.834705908824986 59.21200666036632 15.379595877773278 12.573691553035419 50-51 12.352345595680054 59.617504781190235 15.542805714928566 12.487343908201147 52-53 12.037396425738732 59.822468231601334 15.18920408840587 12.950931254254069 54-55 12.018541124055645 59.64605354199598 14.854275540454639 13.481129793493732 56-57 12.719458617178667 59.35466087631562 14.396935314203423 13.528945192302283 58-59 12.415899732229247 58.58722801017079 15.13467293715263 13.862199320447335 60-61 12.805440554405545 58.37177121771218 14.787710377103771 14.035077850778507 62-63 12.772205376882443 58.04197771164654 14.91103222866658 14.27478468280444 64-65 12.631584869779505 58.02674738518148 15.208988561751353 14.132679183287667 66-67 13.301789662602747 57.7749897323664 14.928857957838002 13.994362647192856 68-69 13.843955190709556 57.314705931998354 14.66373339484952 14.177605482442567 70-71 13.301041332635736 57.57483699290589 14.825628822017068 14.298492852441308 72-73 14.0850537211003 56.82567362322102 14.801710074815775 14.287562580862911 74-75 14.358195522555969 56.550230622117226 15.182810214872314 13.908763640454493 76-77 14.43019462256722 56.257171785352675 16.174485318933513 13.138148273146586 78-79 14.514931250984539 55.96799963993969 16.759682246776336 12.757386862299436 80-81 14.002419059942056 56.5120531068043 16.531743129588477 12.953784703665159 82-83 14.019611704012917 56.017687664204416 16.30258057619929 13.660120055583372 84-85 14.802209671249521 55.146711370131186 16.32726536306564 13.723813595553654 86-87 14.97473923463819 54.45635907418451 16.92808839580075 13.64081329537655 88-89 15.48865496812814 53.67693805100623 17.30194719341971 13.53245978744592 90-91 15.485279307764582 53.80633836494264 17.202365212694733 13.506017114598043 92-93 14.527726515731196 54.577013098078055 16.92453976684521 13.970720619345547 94-95 14.252199743457028 55.08416409748633 16.728176969642412 13.935459189414226 96-97 14.361423821499098 55.40965529657322 15.96145082617428 14.267470055753403 98-99 13.989354519265396 55.93491177529708 15.590677889809147 14.485055815628375 100-101 13.003021080050184 57.946228150615184 15.316369528160179 13.73438124117445 102-103 12.438745829652925 59.47519733547876 15.185970755529048 12.900086079339271 104-105 11.827981186848797 60.94020748475369 14.85248779170511 12.379323536692397 106-107 10.914195374168031 62.70190895741556 14.665158854738076 11.71873681367833 108-109 10.736367165304275 63.60301770454174 14.164195982019793 11.496419148134187 110-111 10.494219571882647 64.12534105932323 13.96247644229417 11.417962926499959 112-113 10.585329627670946 64.48696454491241 13.41408526774161 11.513620559675042 114-115 10.64995555955582 64.7384764240631 13.42663951486786 11.184928501513225 116-117 10.606077652644489 64.35651361902725 13.596525769832251 11.440882958496 118-119 10.38782020625981 64.66295706577696 13.729274152258675 11.219948575704553 120-121 10.865700526576354 64.43921868670958 13.507583599621945 11.187497187092129 122-123 10.61099578642994 64.02770042585747 13.493550256245815 11.86775353146676 124-125 10.712377009062934 64.17637561389988 13.589900819658299 11.521346557378893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10409.0 1 9331.0 2 7144.5 3 4517.5 4 2190.5 5 1036.0 6 591.0 7 550.5 8 666.0 9 661.5 10 462.5 11 282.0 12 216.5 13 186.0 14 175.0 15 181.5 16 192.5 17 190.0 18 193.0 19 211.5 20 220.5 21 245.5 22 299.5 23 357.5 24 396.0 25 446.5 26 552.0 27 666.5 28 767.0 29 851.0 30 964.5 31 1138.0 32 1298.0 33 1445.0 34 1566.0 35 1571.5 36 1671.0 37 1840.5 38 1897.5 39 1875.5 40 1780.0 41 1678.5 42 1611.5 43 1550.0 44 1476.5 45 1428.0 46 1423.0 47 1357.5 48 1798.5 49 2100.5 50 2506.5 51 2522.5 52 1654.5 53 1331.0 54 1244.5 55 1227.5 56 1128.5 57 972.5 58 888.5 59 824.0 60 776.5 61 704.0 62 559.5 63 388.5 64 298.5 65 283.0 66 244.0 67 192.0 68 131.5 69 83.5 70 73.5 71 61.0 72 58.5 73 71.5 74 64.0 75 38.5 76 22.5 77 18.0 78 14.5 79 8.5 80 8.0 81 10.0 82 6.5 83 2.0 84 1.0 85 1.5 86 1.5 87 2.0 88 2.0 89 1.5 90 2.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047244625923801166 2 0.22497440916095796 3 0.028121801145119744 4 0.11473694867208854 5 0.026996929099314955 6 0.03824564955736285 7 0.047244625923801166 8 0.10461310025984545 9 0.12598566913013645 10-11 0.05286898615282511 12-13 0.03599590546575327 14-15 0.014623336595462265 16-17 0.036558341488655666 18-19 0.061305526496361035 20-21 0.19122824778681427 22-23 0.11136233253467417 24-25 0.08099078729794486 26-27 0.014060900572559872 28-29 0.008436540343535923 30-31 0.007311668297731133 32-33 0.007874104320633528 34-35 0.011248720458047898 36-37 0.004499488183219159 38-39 0.007874104320633528 40-41 0.005061924206121554 42-43 0.011811156480950291 44-45 0.011811156480950291 46-47 0.021372568870291004 48-49 0.016873080687071846 50-51 0.010123848412243107 52-53 0.015185772618364662 54-55 0.01743551670997424 56-57 0.01743551670997424 58-59 0.019122824778681425 60-61 0.012373592503852688 62-63 0.02081013284738861 64-65 0.03430859739704609 66-67 0.030933981259631718 68-69 0.037683213534460455 70-71 0.02530962103060777 72-73 0.015748208641267056 74-75 0.010123848412243107 76-77 0.010123848412243107 78-79 0.028121801145119744 80-81 0.024184748984802978 82-83 0.026434493076412557 84-85 0.019122824778681425 86-87 0.02924667319092453 88-89 0.030933981259631718 90-91 0.030933981259631718 92-93 0.03487103341994848 94-95 0.028121801145119744 96-97 0.028684237168022136 98-99 0.03937052160316764 100-101 0.026434493076412557 102-103 0.030933981259631718 104-105 0.028121801145119744 106-107 0.0320588533054365 108-109 0.026434493076412557 110-111 0.024184748984802978 112-113 0.02924667319092453 114-115 0.017997952732876635 116-117 0.017997952732876635 118-119 0.03430859739704609 120-121 0.025872057053510162 122-123 0.0219350048931934 124-125 0.023059876938998188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 88899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66982755711538 #Duplication Level Percentage of deduplicated Percentage of total 1 85.13379583746283 40.5831336685452 2 8.195290008966918 7.813361230160069 3 2.8764925197036195 4.113657071508116 4 1.2341309169852281 2.35323231982362 5 0.6913964793052999 1.647937547104017 6 0.4035112558402945 1.154118718995714 7 0.2147340601255368 0.716543493177651 8 0.19821605550049554 0.7559140147808187 9 0.12506489216102695 0.5365639658488847 >10 0.7574684978054651 6.971956939898087 >50 0.080230308178772 2.701942654023105 >100 0.07079144839303413 7.304919065456304 >500 0.007079144839303412 2.488216965320195 >1k 0.009438859785737884 8.333052115321882 >5k 0.0 0.0 >10k+ 0.002359714946434471 12.525450230036334 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11135 12.525450230036334 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2874 3.2328822596429654 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1594 1.7930460410128346 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1590 1.7885465528296156 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1350 1.5185772618364661 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 892 1.0033858648578724 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 686 0.7716622234220858 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 634 0.7131688770402367 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 496 0.5579365347191757 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.4938188281083027 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 426 0.47919549151284035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 362 0.4072036805813339 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 342 0.3847062396652381 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.33296212555821775 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 270 0.3037154523672932 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 236 0.2654698028099304 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 235 0.2643449307641256 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.26322005871832077 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.2587205705351016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 214 0.24072261780222498 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.22272466506934835 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.21260081665710526 No Hit AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 182 0.20472671233647172 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 181 0.20360184029066694 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.20360184029066694 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 180 0.20247696824486217 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 167 0.1878536316493999 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 157 0.176604911191352 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 155 0.1743551670997424 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.15973183050428014 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 136 0.1529825982294514 No Hit AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 135 0.1518577261836466 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 133 0.14960798209203702 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.14398362186301308 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 122 0.13723438958818435 No Hit AAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.13161002935916039 No Hit GAACAAAAAACACCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 110 0.12373592503852687 No Hit AACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.11361207662628377 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 99 0.11136233253467417 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 96 0.10798771639725982 No Hit AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.10686284435145503 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.10461310025984545 No Hit AAACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.10123848412243108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.002812180114511974 0.0 22-23 0.0 0.0 0.0 0.007874104320633528 0.0 24-25 0.0 0.0 0.0 0.015748208641267056 0.0 26-27 0.0 0.0 0.0 0.023059876938998188 0.0 28-29 0.0 0.0 0.0 0.04049539364897243 0.0 30-31 0.0 0.0 0.0 0.08492783945826163 0.0 32-33 0.0 0.0 0.0 0.12204861696981968 0.0 34-35 0.0 0.0 0.0 0.16254401061879212 0.0 36-37 0.0 0.0 0.0 0.21316325268000766 0.0 38-39 0.0 0.0 0.0 0.3144017368024387 0.0 40-41 0.0 0.0 0.0 0.4510736903677207 0.0 42-43 0.0 0.0 0.0 0.5534370465359565 0.0 44-45 0.0 0.0 0.0 0.6383648859942181 0.0 46-47 0.0 0.0 0.0 0.7351038819334301 0.0 48-49 0.0 0.0 0.0 0.8577149349261521 0.0 50-51 0.0 0.0 0.0 0.9887625282624102 0.0 52-53 0.0 0.0 0.0 1.0871888322703294 0.0 54-55 0.0 0.0 0.0 1.1754912878660053 0.0 56-57 0.0 0.0 0.0 1.2891033644922891 0.0 58-59 0.0 0.0 0.0 1.4336494223782044 0.0 60-61 0.0 0.0 0.0 1.5973183050428013 0.0 62-63 0.0 0.0 0.0 1.7413019269058143 0.0 64-65 0.0 0.0 0.0 1.8498520793259767 0.0 66-67 0.0 0.0 0.0 1.976962620501918 0.0 68-69 0.0 0.0 0.0 2.147943171464246 0.0 70-71 0.0 0.0 0.0 2.3335470590220364 0.0 72-73 0.0 0.0 0.0 2.4983408137324377 0.0 74-75 0.0 0.0 0.0 2.668196492648961 0.0 76-77 0.0 0.0 0.0 2.8408643516799965 0.0 78-79 0.0 0.0 0.0 3.0304052913981034 0.0 80-81 0.0 0.0 0.0 3.2255705913452344 0.0 82-83 0.0 0.0 0.0 3.451669872551997 0.0 84-85 0.0 0.0 0.0 3.674956973644248 0.0 86-87 0.0 0.0 0.0 3.9072430511029372 0.0 88-89 0.0 0.0 0.0 4.148528104928064 0.0 90-91 0.0 0.0 0.0 4.388688286707387 0.0 92-93 0.0 0.0 0.0 4.662032193837951 0.0 94-95 0.0 0.0 0.0 4.942125333243343 0.0 96-97 0.0 0.0 0.0 5.207595136053274 0.0 98-99 0.0 0.0 0.0 5.516372512626688 0.0 100-101 0.0 0.0 0.0 5.888705159788074 0.0 102-103 0.0 0.0 0.0 6.240227674102071 0.0 104-105 0.0 0.0 0.0 6.584438520118336 0.0 106-107 0.0 0.0 0.0 6.875217943958875 0.0 108-109 0.0 0.0 0.0 7.186807500646801 0.0 110-111 0.0 0.0 0.0 7.579950280655575 0.0 112-113 0.0 0.0 0.0 7.994465629534641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAAGA 60 0.0 89.64027 2 ATGGATA 20 8.385213E-4 89.43793 5 GTACAAA 65 1.8189894E-12 82.744865 1 GTGTAAG 30 4.1516498E-5 79.68024 1 CACTACC 25 0.002024435 71.63119 4 ACTACCT 25 0.0020335314 71.55034 5 GAACAAA 610 0.0 70.536606 1 AGAACAA 230 0.0 70.15325 2 GAGAACA 355 0.0 65.65203 1 GAACAAG 80 5.4205884E-10 59.760185 1 CTAGTAA 30 0.004151295 59.76018 1 TAGTAAC 30 0.004151295 59.76018 2 CATGGAT 30 0.004169938 59.692654 4 AGCATCT 40 1.7447844E-4 59.625286 6 ACTTGGT 15 0.004242652 59.457516 104-105 AGGTGTT 15 0.004242652 59.457516 38-39 GAAACAA 215 0.0 58.370407 1 GTACATA 45 3.0876775E-4 53.12016 1 TGTAAGC 45 3.0876775E-4 53.12016 2 GCATCTG 45 3.1223617E-4 53.00025 7 >>END_MODULE