##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940003_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88899 Sequences flagged as poor quality 0 Sequence length 125 %GC 29 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8414267877029 34.0 33.0 34.0 31.0 34.0 2 32.78689299092228 34.0 31.0 34.0 31.0 34.0 3 32.82409250947705 34.0 31.0 34.0 31.0 34.0 4 36.195367776915376 37.0 35.0 37.0 35.0 37.0 5 36.3192386865994 37.0 37.0 37.0 35.0 37.0 6 36.514887681526226 37.0 37.0 37.0 35.0 37.0 7 36.47909425302872 37.0 37.0 37.0 35.0 37.0 8 36.51846477463189 37.0 37.0 37.0 35.0 37.0 9 38.29795610749277 39.0 39.0 39.0 37.0 39.0 10-11 38.3065276324818 39.0 39.0 39.0 37.0 39.0 12-13 38.274620636902554 39.0 39.0 39.0 37.0 39.0 14-15 39.25296685002081 41.0 38.5 41.0 36.0 41.0 16-17 39.381624090259734 40.5 39.0 41.0 36.5 41.0 18-19 39.76845071373131 41.0 40.0 41.0 37.0 41.0 20-21 39.745267100867274 41.0 40.0 41.0 37.0 41.0 22-23 39.4236099393694 41.0 40.0 41.0 35.0 41.0 24-25 39.24243804767208 41.0 39.0 41.0 35.0 41.0 26-27 38.85042013970911 41.0 37.5 41.0 35.0 41.0 28-29 38.45524696565765 40.5 36.5 41.0 35.0 41.0 30-31 37.3332095974083 38.0 35.0 41.0 35.0 41.0 32-33 36.046119753877996 35.0 35.0 41.0 33.5 41.0 34-35 35.485916601986524 35.0 35.0 41.0 33.0 41.0 36-37 35.13618825858559 35.0 35.0 40.5 33.0 41.0 38-39 34.848901562447274 35.0 35.0 40.0 33.0 41.0 40-41 34.40966715036165 35.0 35.0 40.0 30.5 41.0 42-43 33.79726993554483 35.0 35.0 40.0 23.0 41.0 44-45 32.79120125085772 35.0 35.0 40.0 10.5 41.0 46-47 31.12952901607442 35.0 33.0 40.0 2.0 41.0 48-49 29.839896961720605 35.0 29.5 40.0 2.0 41.0 50-51 29.62837602222747 35.0 29.0 40.0 2.0 41.0 52-53 29.662982710716655 35.0 31.0 40.0 2.0 41.0 54-55 29.60794272151543 35.0 31.0 40.0 2.0 41.0 56-57 29.510500680547587 35.0 31.0 39.5 2.0 41.0 58-59 29.37637656216605 35.0 31.0 39.0 2.0 41.0 60-61 29.189968391095512 35.0 31.0 39.0 2.0 41.0 62-63 28.954110844891392 35.0 30.0 38.0 2.0 41.0 64-65 28.716116041800245 35.0 29.5 37.0 2.0 41.0 66-67 28.4321758399982 35.0 29.0 37.0 2.0 41.0 68-69 28.1958964667769 35.0 28.5 36.0 2.0 40.0 70-71 27.924425471602603 35.0 26.5 35.5 2.0 39.0 72-73 27.63883733225346 35.0 26.0 35.0 2.0 39.0 74-75 27.39547126514359 35.0 25.0 35.0 2.0 38.5 76-77 27.117909087841255 35.0 24.0 35.0 2.0 37.0 78-79 26.87877253962362 35.0 21.5 35.0 2.0 37.0 80-81 26.65247078144861 35.0 18.5 35.0 2.0 36.0 82-83 26.42796881854689 35.0 9.5 35.0 2.0 36.0 84-85 26.19089078617307 35.0 3.5 35.0 2.0 36.0 86-87 25.951624877670167 35.0 2.0 35.0 2.0 35.0 88-89 25.780025647082645 35.0 2.0 35.0 2.0 35.0 90-91 25.59921933880021 35.0 2.0 35.0 2.0 35.0 92-93 25.40318788737781 35.0 2.0 35.0 2.0 35.0 94-95 25.219423165614913 35.0 2.0 35.0 2.0 35.0 96-97 25.02965725148764 35.0 2.0 35.0 2.0 35.0 98-99 24.83225345616936 35.0 2.0 35.0 2.0 35.0 100-101 24.63121632414313 35.0 2.0 35.0 2.0 35.0 102-103 24.07964656520321 33.5 2.0 35.0 2.0 35.0 104-105 24.262022069989538 35.0 2.0 35.0 2.0 35.0 106-107 24.126857444965637 35.0 2.0 35.0 2.0 35.0 108-109 23.93683843462806 35.0 2.0 35.0 2.0 35.0 110-111 23.727764091834555 35.0 2.0 35.0 2.0 35.0 112-113 23.514184636497596 35.0 2.0 35.0 2.0 35.0 114-115 23.241926230891238 35.0 2.0 35.0 2.0 35.0 116-117 22.99008987727646 35.0 2.0 35.0 2.0 35.0 118-119 22.78467136863182 35.0 2.0 35.0 2.0 35.0 120-121 22.549702471343885 34.5 2.0 35.0 2.0 35.0 122-123 22.2851719366922 34.0 2.0 35.0 2.0 35.0 124-125 21.696374537396373 32.5 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12700682182363465 1101 2 0.3063512338940626 1101 3 0.06986500444934762 1101 4 -0.11444334795689315 1101 5 0.027875857833116413 1101 6 -0.019274818753210354 1101 7 -0.02336688303197576 1101 8 0.1089632188752887 1101 9 -0.11995925895277537 1101 10-11 0.15904932000445626 1101 12-13 0.0810083976128837 1101 14-15 0.06255876833235874 1101 16-17 0.3444298516816886 1101 18-19 0.17078654543395544 1101 20-21 0.06732408987462435 1101 22-23 -0.01397585128298573 1101 24-25 0.17722324726297245 1101 26-27 0.10774298962916617 1101 28-29 0.145281779678875 1101 30-31 0.21913423158267165 1101 32-33 0.4310460480335436 1101 34-35 -0.05917027096239025 1101 36-37 0.5632910657077517 1101 38-39 0.7975057758374149 1101 40-41 0.5298822095593536 1101 42-43 0.9787398818282682 1101 44-45 1.1038359495439778 1101 46-47 1.9326049570715718 1101 48-49 2.096018017399068 1101 50-51 1.8394309155617563 1101 52-53 1.662400181120848 1101 54-55 1.313949159360618 1101 56-57 1.6133605283392392 1101 58-59 1.8976140611337904 1101 60-61 1.6707241865697178 1101 62-63 1.5750807634094528 1101 64-65 1.0399995925524372 1101 66-67 1.2072025726870983 1101 68-69 1.1169168598578096 1101 70-71 1.179465195720642 1101 72-73 1.151449581301467 1101 74-75 1.2339140422208565 1101 76-77 1.2734000133820764 1101 78-79 1.1090222182396552 1101 80-81 1.1544424795776393 1101 82-83 0.9411568021658319 1101 84-85 0.7329175119209026 1101 86-87 0.6201349839297166 1101 88-89 0.4209501939076681 1101 90-91 0.6569037621724831 1101 92-93 0.8270991769955174 1101 94-95 0.5886848473717663 1101 96-97 0.7222520696599481 1101 98-99 0.5460348392664081 1101 100-101 0.4450820434361482 1101 102-103 0.5712038439517144 1101 104-105 0.6330994264499878 1101 106-107 0.6507934246172589 1101 108-109 0.488885225514192 1101 110-111 0.5828613399945404 1101 112-113 0.7775846669628734 1101 114-115 0.8371056531501324 1101 116-117 0.43173811447181265 1101 118-119 0.742189920425588 1101 120-121 0.8741355971924527 1101 122-123 1.0475649095874715 1101 124-125 1.0867819556549705 1102 1 0.21406975888657342 1102 2 0.15128130382412763 1102 3 0.15168318626752608 1102 4 0.1595077009941619 1102 5 0.23154718650444295 1102 6 0.1836972092188205 1102 7 0.2640457043806066 1102 8 0.24340377831584448 1102 9 0.2489218599283447 1102 10-11 0.3569514179065578 1102 12-13 0.2635258801303664 1102 14-15 0.07418464245823486 1102 16-17 0.2725767048285448 1102 18-19 0.4440382936857077 1102 20-21 0.5725688451193705 1102 22-23 0.8330521207449877 1102 24-25 0.8345659046056326 1102 26-27 0.8646485840347609 1102 28-29 0.9252642971613909 1102 30-31 0.9628405252889678 1102 32-33 0.9123397543272418 1102 34-35 0.5819136451215243 1102 36-37 0.6778889678056572 1102 38-39 0.10921906255070013 1102 40-41 -0.00892897925184144 1102 42-43 -0.09888249579410768 1102 44-45 0.5584688166768359 1102 46-47 -0.5371502876836693 1102 48-49 -1.117093870712818 1102 50-51 -2.190988664857823 1102 52-53 -1.698001916781248 1102 54-55 -1.2148095818981233 1102 56-57 -1.094331779353066 1102 58-59 -1.0052705542508278 1102 60-61 -0.9562863029407715 1102 62-63 -1.0361080477793578 1102 64-65 -0.7615738340209894 1102 66-67 -1.2337065182219895 1102 68-69 -1.1041495737086215 1102 70-71 -1.0332970420415997 1102 72-73 -0.9771343347824448 1102 74-75 -0.7715929507861397 1102 76-77 -0.9511629236808616 1102 78-79 -0.8600337258162867 1102 80-81 -0.9848057721706098 1102 82-83 -0.9778117293027009 1102 84-85 -0.7096524181490267 1102 86-87 -0.6222601209653895 1102 88-89 -0.93910225364478 1102 90-91 -0.7940577762890584 1102 92-93 -0.9648588649625225 1102 94-95 -0.847591376404452 1102 96-97 -0.5414717065638293 1102 98-99 -0.32205956632799726 1102 100-101 -0.7955648097107009 1102 102-103 -0.6866632889154154 1102 104-105 -0.6175124616618959 1102 106-107 -0.294398883075047 1102 108-109 -0.8330553339263673 1102 110-111 -1.1917015970683984 1102 112-113 -1.2607894589112512 1102 114-115 -1.0926146265701462 1102 116-117 -1.0093458016121026 1102 118-119 -0.8229324571967886 1102 120-121 -0.924378388821534 1102 122-123 -0.6974525729300112 1102 124-125 -0.31146979259678176 1103 1 0.31791591273272957 1103 2 0.35220103626560473 1103 3 0.3516831862675289 1103 4 0.36026020935535996 1103 5 0.3425003637619639 1103 6 0.31471727610845335 1103 7 0.3035105873237498 1103 8 0.15837033349979635 1103 9 0.29624627464406217 1103 10-11 0.29323904332461126 1103 12-13 0.35867638180260997 1103 14-15 0.3639421675418504 1103 16-17 0.4855365710492876 1103 18-19 0.41485769167901765 1103 20-21 0.36554543374813875 1103 22-23 0.5281190103770967 1103 24-25 0.6129939982511132 1103 26-27 0.24094457065683628 1103 28-29 0.5110920563587129 1103 30-31 0.49996427110836805 1103 32-33 0.6176073128556752 1103 34-35 0.5432012705395834 1103 36-37 0.9138839510832426 1103 38-39 1.1567943133868255 1103 40-41 1.2917399170692292 1103 42-43 1.695933557717595 1103 44-45 0.8097648032989184 1103 46-47 0.9286523879016109 1103 48-49 0.9276385707587522 1103 50-51 1.4145297297910027 1103 52-53 1.2216887186702543 1103 54-55 1.3740282107440187 1103 56-57 0.8720561805131517 1103 58-59 1.2780488437424857 1103 60-61 1.4497755699689243 1103 62-63 1.5023534906821823 1103 64-65 1.832489711129515 1103 66-67 1.5989021774301868 1103 68-69 1.5117785199368612 1103 70-71 1.342706302440007 1103 72-73 1.1577736919734072 1103 74-75 1.253281631153662 1103 76-77 1.3672735311686353 1103 78-79 1.5074411905716723 1103 80-81 1.7311223214748743 1103 82-83 1.7235678693595062 1103 84-85 1.4722455751620096 1103 86-87 1.4883168021115338 1103 88-89 1.2662071109037178 1103 90-91 1.1025559360855262 1103 92-93 1.3804588607899824 1103 94-95 1.4689220015219675 1103 96-97 1.811580132901053 1103 98-99 1.7444538115984223 1103 100-101 1.8691927153729075 1103 102-103 1.6858283498805697 1103 104-105 1.664719979809675 1103 106-107 1.6225325550520395 1103 108-109 1.7546955021940356 1103 110-111 2.031082683868057 1103 112-113 2.1289680661723587 1103 114-115 1.7754455740987467 1103 116-117 1.5315404860133164 1103 118-119 1.1637314224018738 1103 120-121 1.4411316446232831 1103 122-123 1.2829404036586176 1103 124-125 1.5948847224533864 1104 1 0.3037566206973281 1104 2 0.16089668843951443 1104 3 0.26672743405513444 1104 4 0.29046413394173953 1104 5 0.31025719331179147 1104 6 0.2944017701446171 1104 7 0.2824255546188681 1104 8 0.14444189948671493 1104 9 0.21029013766830218 1104 10-11 0.3401032218548181 1104 12-13 0.2956906180473169 1104 14-15 0.4598636758142618 1104 16-17 0.46599059182650393 1104 18-19 0.24636981172518801 1104 20-21 0.19986632639916024 1104 22-23 0.17809977220856865 1104 24-25 0.25747264388405 1104 26-27 0.26606961875225466 1104 28-29 0.5429889397754195 1104 30-31 0.7330923973107488 1104 32-33 0.47947726283643277 1104 34-35 0.8006849180963371 1104 36-37 1.0260424735919074 1104 38-39 0.8885757677923678 1104 40-41 0.4880077123751221 1104 42-43 0.587468763566001 1104 44-45 0.5959517982969871 1104 46-47 0.6532002909412462 1104 48-49 0.6542641959992288 1104 50-51 0.14546470478138573 1104 52-53 -0.3509546056852635 1104 54-55 -0.05998487121058105 1104 56-57 0.28636937789676153 1104 58-59 0.10513618502759314 1104 60-61 0.13846352687543018 1104 62-63 0.13067207475297948 1104 64-65 0.21417497469242264 1104 66-67 -0.09875076600960142 1104 68-69 0.2208589354812993 1104 70-71 0.1756035706200727 1104 72-73 0.02699262233123534 1104 74-75 0.10149248148071166 1104 76-77 0.43426083397741166 1104 78-79 0.5018621216990269 1104 80-81 0.4729460998511712 1104 82-83 0.4392662148770121 1104 84-85 0.44985234699732857 1104 86-87 0.8467238817575513 1104 88-89 1.1264610547282636 1104 90-91 0.9396471250043419 1104 92-93 1.337557744976209 1104 94-95 1.3845818707024193 1104 96-97 1.2307798250903623 1104 98-99 0.8282552736992308 1104 100-101 0.6553797103709869 1104 102-103 0.5969480112888021 1104 104-105 0.34355799443068236 1104 106-107 0.09902351311283297 1104 108-109 0.10606141215940568 1104 110-111 -0.08946368011809014 1104 112-113 -0.31316352290036065 1104 114-115 -0.1023151030847842 1104 116-117 0.07498027054582579 1104 118-119 0.4624232269420787 1104 120-121 0.2725668119953468 1104 122-123 0.0939446360556957 1104 124-125 0.4404022291867449 1105 1 -0.26947147465465804 1105 2 -0.34360781606498847 1105 3 -0.18165014706580962 1105 4 -0.8809462144597546 1105 5 -0.6871118571546049 1105 6 -0.5374566369350262 1105 7 -0.5772571369222348 1105 8 -0.3474659375538707 1105 9 -0.29317793217144583 1105 10-11 -0.21945190849677232 1105 12-13 0.14935401595850095 1105 14-15 -0.08940683363324808 1105 16-17 0.07637088362272237 1105 18-19 0.07631480096220855 1105 20-21 -0.6834949109443826 1105 22-23 -0.9142625015696382 1105 24-25 -0.9577071376674127 1105 26-27 -0.6313233494371815 1105 28-29 -0.6131539287568373 1105 30-31 -0.4262793638309219 1105 32-33 -1.1456124286249363 1105 34-35 -1.279399591191705 1105 36-37 -1.4883878859711999 1105 38-39 -1.0604794821478478 1105 40-41 -0.826669796992654 1105 42-43 -1.4573371042487153 1105 44-45 -2.6165571708491484 1105 46-47 -2.7087545842879663 1105 48-49 -1.9713038499228013 1105 50-51 -1.8269900508592087 1105 52-53 -2.1649544462337786 1105 54-55 -2.5772056317941754 1105 56-57 -2.7033061383274273 1105 58-59 -2.3713048577851303 1105 60-61 -2.4002831844376544 1105 62-63 -2.5066555183268235 1105 64-65 -2.237232184679339 1105 66-67 -1.9071299416454117 1105 68-69 -2.1661298231888715 1105 70-71 -2.5380614218059776 1105 72-73 -2.383276053424165 1105 74-75 -2.614325106018292 1105 76-77 -2.4287703387882758 1105 78-79 -2.598512589295151 1105 80-81 -2.627867102731944 1105 82-83 -2.544469733460705 1105 84-85 -2.35713162812824 1105 86-87 -2.772944459149727 1105 88-89 -2.644447021489544 1105 90-91 -2.4903935351248165 1105 92-93 -2.202384852488514 1105 94-95 -2.500094841407922 1105 96-97 -2.573652926245046 1105 98-99 -2.8056441124125477 1105 100-101 -3.142714844360736 1105 102-103 -3.049189276441407 1105 104-105 -3.223411630061065 1105 106-107 -2.911819187995352 1105 108-109 -2.827208140579174 1105 110-111 -2.8675072103740114 1105 112-113 -2.807263817885609 1105 114-115 -2.7999950839506056 1105 116-117 -2.8710055382718416 1105 118-119 -2.4983915701559027 1105 120-121 -2.172342699285842 1105 122-123 -2.0973572853347235 1105 124-125 -2.224151705278693 1106 1 0.13515729204306837 1106 2 0.2902070332671016 1106 3 0.3327176690261453 1106 4 -0.1962615297750716 1106 5 -0.025415678217044047 1106 6 -0.0029738783143358205 1106 7 0.13274596963260166 1106 8 0.1608440966182343 1106 9 0.14414732411932363 1106 10-11 0.34799916326464597 1106 12-13 0.3182341029420428 1106 14-15 0.6943769501505415 1106 16-17 0.7062637074810638 1106 18-19 0.2145953228634241 1106 20-21 0.13158741275863406 1106 22-23 0.25679217379538954 1106 24-25 0.09650224412818176 1106 26-27 -0.11396547432067905 1106 28-29 -0.13590944289165918 1106 30-31 -0.46487830760227666 1106 32-33 -0.41425175760910093 1106 34-35 -0.41841791986520604 1106 36-37 -0.1687622258282957 1106 38-39 -0.3354095846641556 1106 40-41 -0.38790775909268405 1106 42-43 -0.1714952278896007 1106 44-45 -0.3296280002992802 1106 46-47 0.40117862478317434 1106 48-49 1.4594976412235177 1106 50-51 1.250324332489658 1106 52-53 1.252760682688244 1106 54-55 1.3397328584201809 1106 56-57 1.2780157007530342 1106 58-59 1.2286485438924082 1106 60-61 1.3966646254411934 1106 62-63 1.5282155596476983 1106 64-65 1.5172723198251639 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82-83 -1.5238282128191862 2216 84-85 -1.2735260445226544 2216 86-87 -1.294650230855499 2216 88-89 -1.1815473085898311 2216 90-91 -1.1601292048604854 2216 92-93 -1.635188535292194 2216 94-95 -1.7741023654154446 2216 96-97 -1.51880687139899 2216 98-99 -1.4683507751192089 2216 100-101 -2.0586142602601534 2216 102-103 -2.0012237284758605 2216 104-105 -2.3077597144091513 2216 106-107 -2.3321736083497733 2216 108-109 -1.8914344548054878 2216 110-111 -1.850355443222245 2216 112-113 -1.772465283087076 2216 114-115 -1.894812428767949 2216 116-117 -1.939290856557161 2216 118-119 -2.020047841812172 2216 120-121 -1.9250652020083479 2216 122-123 -2.115721803699241 2216 124-125 -2.124656605783592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 26.0 6 161.0 7 283.0 8 333.0 9 505.0 10 819.0 11 1192.0 12 1419.0 13 2410.0 14 4507.0 15 4490.0 16 1478.0 17 963.0 18 745.0 19 677.0 20 645.0 21 742.0 22 699.0 23 802.0 24 871.0 25 932.0 26 1036.0 27 1118.0 28 1161.0 29 1232.0 30 1503.0 31 1623.0 32 1876.0 33 2243.0 34 3587.0 35 20174.0 36 6369.0 37 13901.0 38 8289.0 39 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.344718575269724 16.036090766928798 15.966339284709798 10.652851373091679 2 16.574424046076313 24.483666306695465 42.91756659467242 16.024343052555796 3 18.15206020315189 37.19726881067278 32.7618983340645 11.888772652110822 4 8.742621547334744 21.30063533546614 37.030820528995626 32.92592258820349 5 6.391717774151803 38.33567771338547 32.6990378664266 22.573566646036124 6 18.34117144078666 43.727638892014134 15.582457640467137 22.348732026732073 7 13.929290543200711 45.507823485078575 22.293839075805128 18.26904689591559 8 25.642583156165987 28.399644540433528 19.68751054567543 26.27026175772506 9 28.51775610524303 12.806668241487532 22.8978953644023 35.777680288867145 10-11 21.586856995016817 29.980089764789252 23.523886657892664 24.90916658230126 12-13 30.66176222454696 30.178067244850897 28.346213118257797 10.813957412344346 14-15 24.479465460803834 23.514887681526226 40.840166931011595 11.165479926658342 16-17 10.362883721976624 27.103791943666405 41.07751493267641 21.455809401680558 18-19 9.676711774035704 14.277438441377294 62.51532638162409 13.530523402962913 20-21 10.456248101778423 12.667746543830638 66.99400443199586 9.882000922395077 22-23 9.559725081272004 13.113195873969335 67.23641435786679 10.090664686891866 24-25 9.458486597149575 13.52489904273389 67.33990258608084 9.676711774035704 26-27 10.028796724372603 12.563133443570793 67.45295222668422 9.95511760537239 28-29 9.68739805847085 12.52488779401343 68.08906736858683 9.698646778928897 30-31 10.031102887000342 13.240380883816938 67.44039550723578 9.288120721946939 32-33 9.495607374661132 13.150879087503798 67.46420094714226 9.889312590692809 34-35 9.306066434943025 12.983273152678883 67.48669838805836 10.223962024319734 36-37 10.325821020602149 12.726311467572568 66.64173186274233 10.306135649082943 38-39 10.828885276698182 12.542676033364645 66.0965279847913 10.531910705145872 40-41 10.219462536136515 12.914093521861888 65.62840976838885 11.238034173612752 42-43 10.448995202393743 13.298874559188286 64.4544058673656 11.79772437105238 44-45 10.993936939673112 13.679568949032047 62.595192296876235 12.731301814418611 46-47 11.000123735925039 14.186886241690008 60.272893958312245 14.54009606407271 48-49 11.91027919638237 14.635312380481 57.602533240342865 15.851875182793766 50-51 11.821479794144944 15.634860372901374 55.7760341966872 16.767625636266487 52-53 11.555248090529702 16.274086322680795 54.22501940404278 17.945646182746714 54-55 11.452322298338565 16.236965545169237 53.623212859537226 18.68749929695497 56-57 12.347296902067537 15.691579113140902 52.99556795428468 18.96555603050687 58-59 12.521794886216634 15.168678358099823 53.755469813379534 18.554056942304015 60-61 13.57848320547144 15.17075749735652 53.637877117595444 17.612882179576594 62-63 14.583966073859097 14.968109877501433 54.23795543256954 16.209968616069922 64-65 14.647123669823845 15.409795495961664 54.8999977502306 15.043083083983891 66-67 14.864059213264492 15.54010731279317 54.782955938761965 14.812877535180375 68-69 15.010377002986552 15.681366952198294 54.68202500604622 14.626231038768934 70-71 14.01815543481929 16.294896455528185 54.38250149045546 15.304446619197066 72-73 13.664383176413683 16.17903463481029 54.366190845791294 15.790391342984735 74-75 13.178438452626015 16.21390566823024 54.64909616531119 15.958559713832551 76-77 12.36789263798505 16.37371970459697 56.09507792857906 15.163309728838916 78-79 12.225740591798512 16.183062706344874 56.577445063752485 15.01375163810413 80-81 11.44669793810954 16.861831966613796 56.19242061215537 15.499049483121293 82-83 11.428699985376664 16.96588263085074 54.967434954273955 16.637982429498642 84-85 11.945083437853283 16.733128230510076 54.49079568271681 16.830992648919835 86-87 12.138000078741486 16.284301759872214 54.65446548591933 16.923232675466966 88-89 12.554134467204356 16.009178955893766 54.94043802517463 16.49624855172724 90-91 12.376967119990102 16.689726543605666 54.19352298676026 16.73978334964398 92-93 11.435642172164572 17.458871171855225 53.994206811215165 17.111279844765036 94-95 11.698800872910526 17.743368804697518 53.137865868748456 17.4199644536435 96-97 12.516592049314944 17.41377758779725 52.35213390627461 17.717496456613198 98-99 12.820729142060092 17.184107807736872 51.94659107526519 18.04857197493785 100-101 12.671683595990956 17.753855498936996 51.87853631649399 17.69592458857805 102-103 12.865724023892284 17.64586778253974 51.621503054027606 17.866905139540375 104-105 13.150316651480894 17.702111384829973 51.46908289182106 17.678489071868075 106-107 12.685744496563517 18.522143106221666 51.27166784778231 17.5204445494325 108-109 13.060889323839413 18.564325807939348 50.757601322849524 17.617183545371713 110-111 12.647498847006153 18.730244434695553 50.76322568307855 17.859031035219743 112-113 12.915853473343194 19.174114298891432 49.82423775429282 18.085794473472554 114-115 13.030101914553757 19.461067740556594 49.7328398839119 17.77599046097775 116-117 12.672388580170532 19.12247744606178 50.222164728115374 17.98296924565232 118-119 12.592942552784619 19.644765407934848 50.19629017199294 17.566001867287596 120-121 13.040641627014926 19.75387799637791 49.55567554190711 17.649804834700053 122-123 12.518138561738601 19.982227021676284 49.21427687600535 18.285357540579756 124-125 11.963711219599992 20.36041305766159 49.37568899187833 18.300186730860087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1239.0 1 932.0 2 2014.5 3 2787.0 4 1248.0 5 549.0 6 1573.0 7 4160.5 8 6843.5 9 6689.0 10 3668.5 11 1146.0 12 521.0 13 424.5 14 400.0 15 404.0 16 408.0 17 425.5 18 462.5 19 446.0 20 394.0 21 391.0 22 407.0 23 438.0 24 478.5 25 491.0 26 547.5 27 610.5 28 667.5 29 727.0 30 810.0 31 948.5 32 1043.5 33 1127.0 34 1263.5 35 1308.0 36 1395.5 37 1619.0 38 1718.0 39 1799.5 40 1848.0 41 1824.0 42 1816.5 43 1743.5 44 1642.0 45 1575.0 46 1570.5 47 1572.5 48 2059.5 49 2255.0 50 2762.0 51 2689.5 52 1500.0 53 1147.0 54 1112.5 55 1167.0 56 1077.0 57 841.0 58 716.0 59 697.0 60 666.5 61 599.0 62 486.0 63 335.5 64 245.0 65 230.5 66 208.0 67 150.0 68 91.0 69 63.0 70 54.0 71 46.0 72 41.5 73 51.0 74 42.0 75 20.5 76 16.0 77 14.5 78 8.0 79 2.5 80 1.5 81 0.0 82 1.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013498464549657477 2 0.0033746161374143694 3 0.0 4 0.14285874981720828 5 0.03824564955736285 6 0.019122824778681425 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 5.624360229023949E-4 32-33 0.0 34-35 0.0 36-37 5.624360229023949E-4 38-39 0.0028121801145119746 40-41 0.0 42-43 5.624360229023949E-4 44-45 0.0 46-47 0.0 48-49 0.0011248720458047897 50-51 0.0016873080687071847 52-53 0.0 54-55 0.0 56-57 0.0011248720458047897 58-59 0.0022497440916095794 60-61 0.0011248720458047897 62-63 0.0 64-65 0.0011248720458047897 66-67 0.0 68-69 5.624360229023949E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.003937052160316764 78-79 5.624360229023949E-4 80-81 0.0 82-83 0.0 84-85 5.624360229023949E-4 86-87 5.624360229023949E-4 88-89 0.0 90-91 0.0 92-93 0.0016873080687071847 94-95 0.0011248720458047897 96-97 0.0011248720458047897 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 5.624360229023949E-4 114-115 0.0011248720458047897 116-117 0.0011248720458047897 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0011248720458047897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 88899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.21185840110687 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87157230222923 26.98343063476529 2 11.047543894259222 7.559140147808187 3 4.070493851515749 4.177774778118989 4 1.933320181495364 2.6456990517328656 5 1.1705135792727033 2.0022722415325256 6 0.6082725060827251 1.2486079708433164 7 0.3814033011113303 0.9133961011934891 8 0.2794765568488196 0.7649129911472571 9 0.19727756954034328 0.6074309047345864 >10 1.1803774577497206 7.572638612357845 >50 0.13151837969356217 3.1541412164366305 >100 0.09206286578549353 6.292534224231994 >500 0.009863878477017163 2.172127920449049 >1k 0.01643979746169527 11.241971225773069 >5k 0.009863878477017163 22.663921978874903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8856 9.961866837647218 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6264 7.046198494921203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 5.655856646306483 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3474 3.9078054871258394 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2101 2.363356168235863 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1903 2.1406315031665146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 1.4319621143094974 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1243 1.3982159529353535 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 783 0.8807748118651504 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.6782978436202882 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 545 0.6130552649636104 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 418 0.4701965151464021 No Hit GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.40945342467294343 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 340 0.38245649557362854 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.36333367079494705 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.3217134051001698 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 250 0.2812180114511974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 247 0.27784339531378305 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.262095186672516 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.2609703146267112 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 220 0.24747185007705375 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 215 0.24184748984802978 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.2328485134815915 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 206 0.2317236414357867 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 185 0.2081013284738861 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 152 0.17098055096232803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 146 0.1642313186874993 No Hit GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 146 0.1642313186874993 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 146 0.1642313186874993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 145 0.1631064466416945 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 143 0.1608567025500849 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 140 0.15748208641267056 No Hit GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.15748208641267056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 134 0.15073285413784182 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 121 0.13610951754237957 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 120 0.13498464549657477 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 118 0.1327349014049652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 110 0.12373592503852687 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 107 0.12036130890111249 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 97 0.1091125884430646 No Hit GTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.10573797230565023 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 92 0.10348822821404065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 92 0.10348822821404065 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 89 0.10011361207662628 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.10011361207662628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.002812180114511974 0.0 22-23 0.0 0.0 0.0 0.007874104320633528 0.0 24-25 0.0 0.0 0.0 0.015748208641267056 0.0 26-27 0.0 0.0 0.0 0.023059876938998188 0.0 28-29 0.0 0.0 0.0 0.04049539364897243 0.0 30-31 0.0 0.0 0.0 0.0860527115040664 0.0 32-33 0.0 0.0 0.0 0.12317348901562447 0.0 34-35 0.0 0.0 0.0 0.1642313186874993 0.0 36-37 0.0 0.0 0.0 0.21541299677161724 0.0 38-39 0.0 0.0 0.0 0.3205885330543651 0.0 40-41 0.0 0.0 0.0 0.4589477946883542 0.0 42-43 0.0 0.0 0.0 0.5618735868794924 0.0 44-45 0.0 0.0 0.0 0.6473638623606565 0.0 46-47 0.0 0.0 0.0 0.7457901663685755 0.0 48-49 0.0 0.0 0.0 0.8712133994758096 0.0 50-51 0.0 0.0 0.0 1.0039483008807748 0.0 52-53 0.0 0.0 0.0 1.1063116570490106 0.0 54-55 0.0 0.0 0.0 1.203613089011125 0.0 56-57 0.0 0.0 0.0 1.3205997817748232 0.0 58-59 0.0 0.0 0.0 1.4775194321645913 0.0 60-61 0.0 0.0 0.0 1.6541243433559432 0.0 62-63 0.0 0.0 0.0 1.8110439937457112 0.0 64-65 0.0 0.0 0.0 1.9353423548071407 0.0 66-67 0.0 0.0 0.0 2.0759513605327395 0.0 68-69 0.0 0.0 0.0 2.2559308878615054 0.0 70-71 0.0 0.0 0.0 2.450533751785734 0.0 72-73 0.0 0.0 0.0 2.635575203320622 0.0 74-75 0.0 0.0 0.0 2.8324278113364603 0.0 76-77 0.0 0.0 0.0 3.027030675260689 0.0 78-79 0.0 0.0 0.0 3.2463807241926235 0.0 80-81 0.0 0.0 0.0 3.470792697330679 0.0 82-83 0.0 0.0 0.0 3.71770211138483 0.0 84-85 0.0 0.0 0.0 3.9623617813473717 0.0 86-87 0.0 0.0 0.0 4.213208247561839 0.0 88-89 0.0 0.0 0.0 4.478115614348868 0.0 90-91 0.0 0.0 0.0 4.740773237044285 0.0 92-93 0.0 0.0 0.0 5.033802404976434 0.0 94-95 0.0 0.0 0.0 5.330768625068899 0.0 96-97 0.0 0.0 0.0 5.6097368924284865 0.0 98-99 0.0 0.0 0.0 5.935387349688973 0.0 100-101 0.0 0.0 0.0 6.32234333344582 0.0 102-103 0.0 0.0 0.0 6.694675980607206 0.0 104-105 0.0 0.0 0.0 7.05744721537925 0.0 106-107 0.0 0.0 0.0 7.373536260250397 0.0 108-109 0.0 0.0 0.0 7.699186717510884 0.0 110-111 0.0 0.0 0.0 8.109202578206729 0.0 112-113 0.0 0.0 0.0 8.541715879818671 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAC 15 2.6760512E-4 119.193344 1 GTGTATC 20 5.619815E-6 119.193344 1 ATAACGC 60 0.0 119.193344 3 TATAACG 55 0.0 119.193344 2 TACAACG 15 2.6760512E-4 119.193344 2 TGTATCA 35 7.354174E-9 102.16573 2 TAACGCA 75 0.0 95.354675 4 GTATAAC 80 0.0 81.94543 1 ACAACGC 25 0.0020374137 71.51601 3 GTATCAC 25 0.0020374137 71.51601 1 CATATAG 35 9.033136E-5 68.11049 3 AGGATAT 45 3.96024E-6 66.106735 8 TCACGCA 30 0.004196626 59.596672 4 TATCACG 30 0.004196626 59.596672 2 ATATAGG 40 1.7489407E-4 59.596672 4 GACATAT 40 1.7489407E-4 59.596672 1 TACTAAG 30 0.004196626 59.596672 2 ACCTCGA 15 0.0042317775 59.49606 96-97 CCCGTCA 20 1.7684665E-4 59.49606 90-91 TAGGATA 55 1.3013563E-5 54.087326 7 >>END_MODULE