FastQCFastQC Report
Fri 27 May 2016
SRR940002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443655
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28760.6482514566498744No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7500.16905027555194915No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7410.16702167224532577No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6650.14989124432272824No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6430.14493243623987107No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5430.12239239949961118No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT5420.12216699913220859No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA5340.1203637961929878No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5300.1194621947233774No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT5240.11810979251896181No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5020.11315098443610463No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA4840.10909377782285785No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4760.10729057488363707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA250.002039803471.542591
GCTTTCG150.00423874559.49109372-73
TCCAACG350.00772842851.1076242
GGTATCA7950.048.7449531
GTATCAA14100.043.5513421
GCATATA600.001294877139.7458841
CGCTCGC300.001309313939.6607366-67
TAAGTCG300.0013100439.65625842-43
GTACATG23750.037.1519431
TAGGGTC1951.8189894E-1236.655374
TCAACGC15400.036.357844
AACGCAG15250.036.312576
CAACGCA16250.035.177245
CTAGGGG851.7001166E-435.030313
ATCAACG16150.034.661573
ATAGGGT2750.034.6481633
CGTCTTA952.7284841E-1134.50446714-15
GGTGCTC700.002776879134.0256048
GACATAT902.3800887E-433.080457
TATCAAC18200.032.7613032
Produced by FastQC (version 0.11.5)