##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 443655 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.152363886353136 34.0 31.0 34.0 26.0 34.0 2 31.064493807124904 34.0 31.0 34.0 26.0 34.0 3 31.23282505550484 34.0 31.0 34.0 26.0 34.0 4 34.17874474535393 37.0 35.0 37.0 30.0 37.0 5 34.608641850086215 37.0 37.0 37.0 31.0 37.0 6 34.45214637499859 37.0 37.0 37.0 31.0 37.0 7 34.512468021322874 37.0 37.0 37.0 31.0 37.0 8 34.331593242496986 37.0 37.0 37.0 31.0 37.0 9 35.783171608569724 39.0 39.0 39.0 30.0 39.0 10-11 35.95962966719635 39.0 39.0 39.0 31.0 39.0 12-13 35.941706956982344 39.0 39.0 39.0 30.5 39.0 14-15 37.43709526546528 41.0 39.5 41.0 31.0 41.0 16-17 37.562625238079136 41.0 39.0 41.0 31.0 41.0 18-19 37.66638717021109 41.0 39.5 41.0 31.0 41.0 20-21 37.763509934521196 41.0 40.0 41.0 31.0 41.0 22-23 37.84356200200607 41.0 39.5 41.0 30.0 41.0 24-25 37.98350069310613 41.0 39.0 41.0 30.0 41.0 26-27 38.04132264935592 41.0 39.0 41.0 30.0 41.0 28-29 37.96083894016748 41.0 39.0 41.0 30.0 41.0 30-31 37.86615275382899 41.0 39.0 41.0 30.0 41.0 32-33 37.769435710180204 41.0 39.0 41.0 30.0 41.0 34-35 37.62701761503871 41.0 39.0 41.0 30.0 41.0 36-37 37.431797229829485 41.0 39.0 41.0 30.0 41.0 38-39 37.3020218412956 41.0 38.5 41.0 30.0 41.0 40-41 37.22208360099627 41.0 39.0 41.0 30.0 41.0 42-43 37.049431427573225 41.0 38.0 41.0 30.0 41.0 44-45 36.93185357992134 40.5 38.0 41.0 30.0 41.0 46-47 36.802060159358064 40.0 38.0 41.0 29.0 41.0 48-49 36.66716367447679 40.0 38.0 41.0 29.0 41.0 50-51 36.54322164745129 40.0 38.0 41.0 29.0 41.0 52-53 36.687572550743255 40.0 38.0 41.0 30.0 41.0 54-55 36.528865898051414 40.0 37.5 41.0 30.0 41.0 56-57 36.44305710518309 40.0 37.0 41.0 30.0 41.0 58-59 36.28717697309847 40.0 37.0 41.0 30.0 41.0 60-61 36.087592836776324 40.0 36.5 41.0 30.0 41.0 62-63 35.791457326075445 39.5 36.0 41.0 29.0 41.0 64-65 35.495308291352515 39.0 35.0 41.0 29.0 41.0 66-67 35.160879512233606 39.0 35.0 41.0 28.0 41.0 68-69 34.76438561494855 38.5 35.0 40.5 28.0 41.0 70-71 34.38969582220419 37.0 35.0 40.0 27.0 41.0 72-73 33.96778239848531 37.0 35.0 39.0 26.0 41.0 74-75 33.51701885474073 36.0 35.0 39.0 26.0 41.0 76-77 33.1212958267122 36.0 35.0 38.5 25.0 40.0 78-79 32.715917773945975 35.5 34.0 37.0 24.0 39.0 80-81 32.33385964319122 35.0 34.0 37.0 22.0 39.0 82-83 31.94740958627763 35.0 34.0 36.0 19.0 38.5 84-85 31.637500986126607 35.0 34.0 36.0 17.5 37.0 86-87 31.392283418421975 35.0 34.0 36.0 11.5 37.0 88-89 31.162300661550077 35.0 34.0 35.0 7.0 36.5 90-91 30.971836224093046 35.0 34.0 35.0 2.0 36.0 92-93 30.80345200662677 35.0 33.5 35.0 2.0 36.0 94-95 30.641082597964633 35.0 33.0 35.0 2.0 36.0 96-97 30.5019091411119 35.0 33.0 35.0 2.0 35.5 98-99 30.418444512064553 35.0 33.0 35.0 2.0 35.0 100-101 30.335059900147638 35.0 33.0 35.0 2.0 35.0 102-103 29.679972050354444 34.0 31.0 35.0 2.0 35.0 104-105 30.481135116250236 35.0 33.5 35.0 2.0 35.0 106-107 30.54537760196549 35.0 34.0 35.0 2.0 35.0 108-109 30.518640610384196 35.0 34.0 35.0 2.0 35.0 110-111 30.445590605312688 35.0 34.0 35.0 2.0 35.0 112-113 30.374480170402677 35.0 34.0 35.0 2.0 35.0 114-115 30.29170639348142 35.0 33.0 35.0 2.0 35.0 116-117 30.164063292423165 35.0 33.0 35.0 2.0 35.0 118-119 30.031767927781722 35.0 33.0 35.0 2.0 35.0 120-121 29.854845544398238 35.0 33.0 35.0 2.0 35.0 122-123 29.6779276690221 35.0 33.0 35.0 2.0 35.0 124-125 28.438596431912185 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15580103274678336 1101 2 0.02274737874972743 1101 3 0.004949328008489573 1101 4 -0.12905192487457384 1101 5 -0.32678722335663224 1101 6 0.08739823907416167 1101 7 -7.377416131575387E-4 1101 8 -0.14116654437173537 1101 9 -0.15243843290947723 1101 10-11 -0.12492331452498462 1101 12-13 -0.011457257982755209 1101 14-15 0.01270541489517285 1101 16-17 -0.07594265546868684 1101 18-19 -0.005095900731582503 1101 20-21 -0.16728597398731893 1101 22-23 -0.2567122857899733 1101 24-25 -0.2043651520912917 1101 26-27 -0.11754305148150479 1101 28-29 -0.07285065809351465 1101 30-31 -0.24363841509809703 1101 32-33 -0.15200603579116745 1101 34-35 0.01715851878085317 1101 36-37 -0.04096905748793489 1101 38-39 -0.03099794257764188 1101 40-41 -0.029918159741313843 1101 42-43 -0.055796506175923355 1101 44-45 -0.13936567093091412 1101 46-47 -0.11431721738027534 1101 48-49 -0.3135625137611626 1101 50-51 -0.1471816602521585 1101 52-53 -0.1742018007552204 1101 54-55 -0.12908666698533722 1101 56-57 -0.09334622347583377 1101 58-59 -0.13246592241851118 1101 60-61 -0.3015914540731188 1101 62-63 -0.14454314775503008 1101 64-65 -0.11890548542835688 1101 66-67 -0.29845365936989054 1101 68-69 -0.12478883510863881 1101 70-71 -0.11081401649167333 1101 72-73 -0.25911369736473944 1101 74-75 -0.2961708675345278 1101 76-77 -0.3298242750313136 1101 78-79 -0.41407451983370436 1101 80-81 -0.27891769110449616 1101 82-83 -0.3010219837816983 1101 84-85 -0.1864055680547665 1101 86-87 -0.1533860972862513 1101 88-89 -0.11339107090682887 1101 90-91 -0.16604369559085796 1101 92-93 -0.10766985280500307 1101 94-95 0.05698311853530669 1101 96-97 -0.23123938215270812 1101 98-99 -0.10650858132880714 1101 100-101 0.18063746375893786 1101 102-103 -0.0374795756403401 1101 104-105 0.019766359666256506 1101 106-107 0.04040946435671344 1101 108-109 0.07501751086595476 1101 110-111 0.05972557418128588 1101 112-113 0.20944174359009793 1101 114-115 0.22777611916191276 1101 116-117 -0.11835080987757252 1101 118-119 4.749310155425235E-4 1101 120-121 0.010205330959127679 1101 122-123 -0.06813226953511986 1101 124-125 -0.2048409915845113 1102 1 -0.2310326844602706 1102 2 -0.2344087856722119 1102 3 -0.19207194262654426 1102 4 -0.4404877802874836 1102 5 -0.27013673553980055 1102 6 -0.3076528538736767 1102 7 -0.07104920198197817 1102 8 -0.14626183149379557 1102 9 -0.1881544695815336 1102 10-11 -0.2213748235003905 1102 12-13 -0.098964874649063 1102 14-15 -0.06608768493043016 1102 16-17 -0.059035407203886336 1102 18-19 -0.05604877195213476 1102 20-21 -0.38331389125806936 1102 22-23 -0.33119102566067227 1102 24-25 -0.4273303640622359 1102 26-27 -0.3748970741318942 1102 28-29 -0.28050199337391035 1102 30-31 -0.3712517195756462 1102 32-33 -0.21329305979329405 1102 34-35 -0.26387895871933864 1102 36-37 -0.3486893805046307 1102 38-39 -0.2493546336876804 1102 40-41 -0.35441966206307995 1102 42-43 -0.3898442302810068 1102 44-45 -0.3982167624779862 1102 46-47 -0.5203503174860273 1102 48-49 -0.5116028418206113 1102 50-51 -0.4436713401070378 1102 52-53 -0.46700046162271747 1102 54-55 -0.43130197876087806 1102 56-57 -0.5063061125351425 1102 58-59 -0.5632137915028963 1102 60-61 -0.5219810071586579 1102 62-63 -0.46821819800751996 1102 64-65 -0.5545682929841007 1102 66-67 -0.5272670703056761 1102 68-69 -0.5643212593446876 1102 70-71 -0.4492443879048338 1102 72-73 -0.47403676028015695 1102 74-75 -0.5939247389021673 1102 76-77 -0.5576501457826168 1102 78-79 -0.52775370234653 1102 80-81 -0.6068878407763663 1102 82-83 -0.588781388175903 1102 84-85 -0.7565244230684591 1102 86-87 -0.6389599561870654 1102 88-89 -0.4494016186764611 1102 90-91 -0.6025504647936089 1102 92-93 -0.6652150800442982 1102 94-95 -0.7957455740595414 1102 96-97 -0.9266515225935308 1102 98-99 -0.7601393673687724 1102 100-101 -0.602490657323294 1102 102-103 -0.6929891393717007 1102 104-105 -0.5564020544562673 1102 106-107 -0.7740170269340219 1102 108-109 -0.6388374678073774 1102 110-111 -0.8121200753890285 1102 112-113 -0.8947647573659268 1102 114-115 -0.788014018043139 1102 116-117 -0.8657156184278527 1102 118-119 -0.8173519202721131 1102 120-121 -0.9627528771807228 1102 122-123 -0.9356530534038967 1102 124-125 -0.8704763441223484 1103 1 0.1647090737814878 1103 2 0.47406191029848443 1103 3 0.19538226982766815 1103 4 0.2601074578077558 1103 5 0.48792187251881103 1103 6 0.5734368897160635 1103 7 0.4826138016810262 1103 8 0.3890403663084072 1103 9 0.48020633627927367 1103 10-11 0.4291517332761643 1103 12-13 0.39135105941686277 1103 14-15 0.5666046227618793 1103 16-17 0.43116605799757934 1103 18-19 0.4094732060698334 1103 20-21 0.053545083100914326 1103 22-23 0.20601135529170733 1103 24-25 0.30861285938099314 1103 26-27 0.23870182696700226 1103 28-29 0.3316546000326781 1103 30-31 0.31082245624853755 1103 32-33 0.3802051087048781 1103 34-35 0.4792300156396365 1103 36-37 0.5090258209605736 1103 38-39 0.5439832784002334 1103 40-41 0.4795089093654923 1103 42-43 0.38559075140397425 1103 44-45 0.31565686389564007 1103 46-47 0.2661423565066414 1103 48-49 0.4296975244797565 1103 50-51 0.3612279272922265 1103 52-53 0.36903433691208676 1103 54-55 0.48753959633069854 1103 56-57 0.42267740394837006 1103 58-59 0.4610536077645051 1103 60-61 0.3445025093248617 1103 62-63 0.2574457214063983 1103 64-65 0.49955258613678666 1103 66-67 0.4810891568005502 1103 68-69 0.3386091435857068 1103 70-71 0.30382337766292977 1103 72-73 0.349475144481751 1103 74-75 0.1915835028560764 1103 76-77 0.2987830044005335 1103 78-79 0.338134576041746 1103 80-81 0.23104714090862188 1103 82-83 0.3387827143882056 1103 84-85 0.20244993590590354 1103 86-87 0.27207484234773105 1103 88-89 0.1620681615433206 1103 90-91 0.25505026780712114 1103 92-93 0.29213382105460184 1103 94-95 0.3104128508488806 1103 96-97 0.40686496092295243 1103 98-99 0.49068480845540563 1103 100-101 0.44377765769502275 1103 102-103 0.4500511536685927 1103 104-105 0.5087994107451976 1103 106-107 0.5734417642747687 1103 108-109 0.5649400047200928 1103 110-111 0.5135667377977811 1103 112-113 0.5230923854912177 1103 114-115 0.5022332347041143 1103 116-117 0.49859298963075815 1103 118-119 0.6850061383359467 1103 120-121 0.8292114085335633 1103 122-123 0.6567004264495822 1103 124-125 0.5316756705296655 1104 1 0.1267217693444529 1104 2 0.3960322873182349 1104 3 0.2226136111972501 1104 4 0.4202029951250168 1104 5 0.3788297976278159 1104 6 0.5195480720705135 1104 7 0.6584409368438884 1104 8 0.3730330567418463 1104 9 0.7327358310215146 1104 10-11 0.5468020923425954 1104 12-13 0.6066450450542078 1104 14-15 0.5538641764936116 1104 16-17 0.6930344857995792 1104 18-19 0.5058408644437833 1104 20-21 0.26871884560411274 1104 22-23 0.2769991085617747 1104 24-25 0.49045756315118894 1104 26-27 0.6619417602832414 1104 28-29 0.5395652822588914 1104 30-31 0.5970833564793665 1104 32-33 0.717580076645369 1104 34-35 0.8154428266168097 1104 36-37 0.8983003144204389 1104 38-39 0.6894709034322943 1104 40-41 0.6044159368084223 1104 42-43 0.6446748114193639 1104 44-45 0.5386210854909166 1104 46-47 0.6846759548651917 1104 48-49 0.6868339697221231 1104 50-51 0.52762636278851 1104 52-53 0.574801841400415 1104 54-55 0.6975213224143104 1104 56-57 0.6435449340843036 1104 58-59 0.7325014148945357 1104 60-61 0.6762157691350694 1104 62-63 0.6112367575695146 1104 64-65 0.4903643327384799 1104 66-67 0.5103674974007077 1104 68-69 0.6109866762864016 1104 70-71 0.5551666708150194 1104 72-73 0.4900993429941849 1104 74-75 0.4179027513813409 1104 76-77 0.4552542149673471 1104 78-79 0.5572516573446435 1104 80-81 0.5624734681720227 1104 82-83 0.6247085928185676 1104 84-85 0.5676974352647051 1104 86-87 0.5897492460409879 1104 88-89 0.7103385513868687 1104 90-91 0.5963236712439013 1104 92-93 0.6355375784282877 1104 94-95 0.6367930124287753 1104 96-97 0.6019406213487031 1104 98-99 0.6041305637772822 1104 100-101 0.5672981116575748 1104 102-103 0.5037444083492808 1104 104-105 0.6775221601681274 1104 106-107 0.6899283634027498 1104 108-109 0.5016817974874712 1104 110-111 0.5197253682158376 1104 112-113 0.5432962839267148 1104 114-115 0.5461643708928818 1104 116-117 0.6764286526212686 1104 118-119 0.6162240788335076 1104 120-121 0.7926972382334831 1104 122-123 0.6568575180115239 1104 124-125 0.8548066015376179 1105 1 -0.2121997932135855 1105 2 -0.2936105034946195 1105 3 -0.12148965135460088 1105 4 -0.1378605224878129 1105 5 -0.5199599008802025 1105 6 -0.531550795012997 1105 7 -0.5390610751662663 1105 8 -0.457326628765486 1105 9 -0.5532296243118608 1105 10-11 -0.667184473620388 1105 12-13 -0.8467954923072725 1105 14-15 -0.6038387269918175 1105 16-17 -0.6508536464359196 1105 18-19 -0.6244553653587346 1105 20-21 -0.6252110745345618 1105 22-23 -0.5573815018511539 1105 24-25 -0.5150940371707335 1105 26-27 -0.4189952173679714 1105 28-29 -0.3776126413466301 1105 30-31 -0.46069847971205746 1105 32-33 -0.44011508833946067 1105 34-35 -0.5169830385475578 1105 36-37 -0.5846626026847446 1105 38-39 -0.7006903669199716 1105 40-41 -0.673631484723181 1105 42-43 -0.5665336328324813 1105 44-45 -0.7793554513752952 1105 46-47 -0.9128601065475479 1105 48-49 -0.8565093720719688 1105 50-51 -0.8730701022644212 1105 52-53 -0.901224283777573 1105 54-55 -0.718895132733337 1105 56-57 -0.8201181625540883 1105 58-59 -0.8072961459300814 1105 60-61 -0.7519260621037063 1105 62-63 -0.7346417170172472 1105 64-65 -0.8861616995774995 1105 66-67 -0.9017618284211224 1105 68-69 -0.8095435657739003 1105 70-71 -0.7846618440119428 1105 72-73 -0.7514177126611088 1105 74-75 -0.6458857089665813 1105 76-77 -0.7420729069295149 1105 78-79 -0.7210403008055479 1105 80-81 -0.6753260118007418 1105 82-83 -0.6578921624590883 1105 84-85 -0.6109737094142957 1105 86-87 -0.552001512332069 1105 88-89 -0.43950203550101463 1105 90-91 -0.490516234655928 1105 92-93 -0.4877614991424437 1105 94-95 -0.4984233560476703 1105 96-97 -0.5288493247913308 1105 98-99 -0.49195065952488903 1105 100-101 -0.4154280073296093 1105 102-103 -0.3899734768732763 1105 104-105 -0.3926784217671191 1105 106-107 -0.4430299106020783 1105 108-109 -0.40190112828483393 1105 110-111 -0.3666672523499983 1105 112-113 -0.34304061943489117 1105 114-115 -0.37960174066534336 1105 116-117 -0.34484912859584327 1105 118-119 -0.21342366461971807 1105 120-121 -0.2740829264418103 1105 122-123 -0.2126961252745545 1105 124-125 -0.3060706348890534 1106 1 -0.2721106541096674 1106 2 -0.121771423034847 1106 3 -0.0034272539818580583 1106 4 -0.20239254219224279 1106 5 -0.04132982816145869 1106 6 -0.2426345388553628 1106 7 0.04826006018442541 1106 8 0.18079206698867978 1106 9 -0.13399434399283905 1106 10-11 -0.3185098313496866 1106 12-13 -0.22867445078721715 1106 14-15 -0.05771510512927591 1106 16-17 -0.23499040458744958 1106 18-19 0.07418409042357865 1106 20-21 -0.25079165159296934 1106 22-23 -0.08119102566067227 1106 24-25 -0.1503157120475791 1106 26-27 -0.0477777648697284 1106 28-29 -0.1292722707156173 1106 30-31 0.010354769173702039 1106 32-33 0.07680374816064983 1106 34-35 0.1313983829865748 1106 36-37 0.1929118753823431 1106 38-39 0.21715504710771683 1106 40-41 0.19404972569201817 1106 42-43 0.15731694188016832 1106 44-45 0.14312285029019733 1106 46-47 0.0983702476631052 1106 48-49 0.07408527958179434 1106 50-51 -0.09719044005471034 1106 52-53 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7265.0 3 1186.0 4 1115.0 5 1630.0 6 3542.0 7 3797.0 8 2314.0 9 1879.0 10 1945.0 11 2083.0 12 2305.0 13 2231.0 14 2278.0 15 2212.0 16 1896.0 17 1748.0 18 1474.0 19 1108.0 20 935.0 21 777.0 22 775.0 23 1048.0 24 1936.0 25 3427.0 26 4447.0 27 2621.0 28 2275.0 29 3014.0 30 3396.0 31 4156.0 32 5506.0 33 7705.0 34 11582.0 35 20090.0 36 48074.0 37 170487.0 38 109246.0 39 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.79269189306221 24.008903964384142 13.929020879661161 22.269383262892482 2 14.098089577728231 24.74038633134317 39.87941546545224 21.28210862547636 3 16.595381052982834 30.717147553328928 30.713089120920994 21.974382272767244 4 10.931040707645094 21.33766585431898 40.04964346962722 27.6816499684087 5 10.84211309490259 42.94729437546642 34.587713819979754 11.622878709651237 6 25.426480702664307 43.43304281155923 18.692313763516026 12.448162722260433 7 23.995751647251424 37.984197210158165 22.703206108247308 15.316845034343107 8 23.014196879033204 39.26434354100669 20.68543037392034 17.036029206039764 9 24.285817456341093 20.81228813750581 22.093731804086715 32.808162602066375 10-11 20.96617061053467 32.02877110531289 29.00180636897285 18.00325191517959 12-13 21.679296886449777 30.829197791271056 28.00244863171993 19.489056690559238 14-15 20.458277868012637 31.662178719125873 25.580286544836454 22.299256868025036 16-17 19.347979996121882 33.85270814450051 27.0785138687843 19.720797990593308 18-19 19.0321867698664 33.146712312702405 28.94170658439555 18.87939433303564 20-21 20.29950308637445 32.23118847151356 28.687644453276455 18.781663988835533 22-23 20.78259084006327 32.09576933404638 27.588797952925407 19.53284187296494 24-25 19.880763465709343 32.76771545642487 28.05960924432775 19.291911833538038 26-27 19.4041257170296 32.43887565700708 28.713759641645826 19.443238984317496 28-29 19.638705081865037 31.88042193151221 29.033432403158965 19.447440583463795 30-31 20.261532201800428 31.778123672135727 28.43740032212508 19.522943803938762 32-33 19.472690018010542 32.630756781003946 28.764237685092723 19.132315515892785 34-35 19.85129030512876 32.42838400779052 28.347602430931257 19.372723256149463 36-37 20.528180165518393 31.47509278429525 27.690462800062665 20.306264250123693 38-39 19.402482087649638 32.911950245537554 28.043577984236457 19.64198968257636 40-41 19.413227900245815 32.204390816231076 29.249905044851516 19.132476238671597 42-43 19.286717416835923 32.05905624522386 28.689099233337316 19.965127104602907 44-45 19.353624338385796 32.136754641443424 28.70627936124361 19.80334165892717 46-47 19.775552439480766 32.07857468134128 27.957428005897494 20.18844487328046 48-49 19.160805266242843 32.06310022994725 28.377519274989854 20.398575228820054 50-51 19.104168075561866 31.75424359234463 27.980320551836073 21.161267780257432 52-53 19.250056639693774 32.12524529777826 28.429857515946473 20.1948405465815 54-55 20.017538480960155 31.755922055203385 27.73323023652152 20.493309227314942 56-57 19.65702331222511 32.35768857067497 27.79905632942354 20.186231787676377 58-59 19.11607727588854 31.2574183432733 28.3259257923326 21.300578588505555 60-61 19.801180070668988 32.09217400125108 27.517624980980237 20.589020947099698 62-63 19.58799957165482 31.627880762228973 28.043195229587383 20.740924436528825 64-65 19.669702678296634 31.291325214003308 28.1018783137678 20.93709379393226 66-67 19.646967842012586 31.662191198320745 27.576438068311205 21.114402891355468 68-69 19.81359576187969 31.117016179310642 28.013510755955206 21.055877302854462 70-71 19.772670652013396 31.51227111780972 28.433528767883047 20.281529462293832 72-73 20.28479257686221 30.904717461777427 28.010013841735308 20.800476119625056 74-75 19.73292096281903 31.87974229848472 27.37886991844261 21.008466820253645 76-77 19.719573499244834 31.856698451342396 28.071616960843986 20.35211108856879 78-79 19.93105531807442 31.262738307811627 28.004098804177264 20.80210756993669 80-81 20.28512232733044 31.105657808117883 27.868582662253928 20.74063720229775 82-83 20.457573660206016 31.354482062057414 27.879280453245016 20.308663824491553 84-85 20.004689083340566 30.87603574073427 27.863074257721486 21.256200918203675 86-87 20.17240679616904 30.95882257453601 28.325261980773046 20.543508648521904 88-89 20.87018279588075 30.714330002085553 27.952494490195086 20.462992711838613 90-91 20.42955235422963 30.57223669570256 28.361232516743456 20.63697843332435 92-93 20.86903387568094 30.56443684837115 28.113049713193117 20.45347956275479 94-95 20.53117197143485 30.1525766687897 28.313652990942344 21.0025983688331 96-97 20.686560871373832 30.346684944293443 27.82137377558877 21.14538040874395 98-99 19.859483298985808 30.988757762014497 27.62533088616614 21.52642805283356 100-101 19.661176672163798 31.42045858043193 27.873849237258618 21.04451551014565 102-103 20.02885970351164 31.512767036807393 27.495969787497888 20.962403472183077 104-105 19.26413246021538 31.948316347063262 27.791349007822213 20.996202184899147 106-107 19.09810884698853 32.64428849243257 27.08108565147544 21.176517009103463 108-109 19.099929208168565 33.21602330268693 26.64128832115143 21.042759167993076 110-111 18.993328989699272 32.97314236373351 26.972840263233245 21.06068838333397 112-113 18.831827640861345 32.8361203862569 27.7924827354528 20.539569237428953 114-115 18.649233543733093 33.637173128944994 26.734445446348058 20.97914788097385 116-117 18.55213208176662 33.88145733582204 26.602330006041424 20.964080576369916 118-119 19.305646525921574 32.61269772197677 26.89684016860551 21.184815583496142 120-121 18.778674831079176 33.184571785431665 26.81867474089789 21.21807864259127 122-123 19.092417499673108 33.26378462218255 26.612245081993084 21.031552796151264 124-125 19.43783457195643 32.92374042006851 26.354886089133522 21.283538918841536 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2326.0 1 1576.5 2 772.0 3 627.5 4 456.5 5 332.0 6 282.5 7 279.5 8 315.0 9 316.0 10 259.5 11 206.0 12 182.0 13 186.0 14 188.0 15 223.5 16 243.0 17 263.5 18 316.5 19 350.5 20 436.5 21 574.0 22 703.0 23 843.5 24 1011.5 25 1503.0 26 2755.0 27 4407.0 28 5020.5 29 5757.0 30 7876.5 31 9764.5 32 12308.5 33 15457.5 34 18863.0 35 20963.5 36 22677.0 37 25988.0 38 25749.5 39 23027.0 40 20718.5 41 18927.5 42 17885.5 43 17208.5 44 16536.0 45 15909.0 46 14336.5 47 11843.5 48 10955.5 49 10320.0 50 9128.0 51 7818.0 52 6926.5 53 6840.5 54 6517.5 55 5824.5 56 5281.0 57 4687.5 58 4064.5 59 3580.0 60 3121.0 61 2430.0 62 1737.5 63 1317.0 64 1026.0 65 829.5 66 702.5 67 584.5 68 428.0 69 309.5 70 254.0 71 213.5 72 181.0 73 168.5 74 129.5 75 90.5 76 84.5 77 85.5 78 69.5 79 45.5 80 38.0 81 34.5 82 30.0 83 26.5 84 22.0 85 17.5 86 12.5 87 15.0 88 13.5 89 8.5 90 8.0 91 9.5 92 6.5 93 6.0 94 7.5 95 7.5 96 6.5 97 4.5 98 6.0 99 8.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0579278944224679 2 0.2204415593197417 3 0.030203649231948248 4 0.11157318186428644 5 0.02614644261870147 6 0.04508007348051977 7 0.04260066943909118 8 0.1034587686377929 9 0.12644960611285797 10-11 0.05048968229818215 12-13 0.03200685217116904 14-15 0.013411321860454633 16-17 0.03178145180376644 18-19 0.05477228927883152 20-21 0.18516640182123498 22-23 0.10954457855766304 24-25 0.07539642289616932 26-27 0.016116126269285817 28-29 0.0064239104709740685 30-31 0.008677914145000055 32-33 0.00586040955246757 34-35 0.008790614328701356 36-37 0.004169906796948079 38-39 0.008227113410194858 40-41 0.005522309001363673 42-43 0.009917616165714349 44-45 0.009692215798311752 46-47 0.01735582829000011 48-49 0.016905027555194914 50-51 0.01014301653311695 52-53 0.01386212259525983 54-55 0.013298621676753334 56-57 0.015327224983376722 58-59 0.017017727738896215 60-61 0.008452513777597457 62-63 0.0197225321477274 64-65 0.032795753457078136 66-67 0.028400446292727456 68-69 0.03527515749850672 70-71 0.02558294170019497 72-73 0.015552625350779323 74-75 0.009241415063506554 76-77 0.01014301653311695 78-79 0.02501944078168847 80-81 0.020511433433636497 82-83 0.02208923600545469 84-85 0.016116126269285817 86-87 0.02501944078168847 88-89 0.028738546843831356 90-91 0.027836945374220958 92-93 0.03403545547779243 94-95 0.024230539495779378 96-97 0.02828774610902616 98-99 0.03358465474298723 100-101 0.0203987332499352 102-103 0.02930204776233785 104-105 0.024906740597987174 106-107 0.03211955235487034 108-109 0.022765437107662486 110-111 0.021187634535844293 112-113 0.02614644261870147 114-115 0.012397020207142938 116-117 0.011495418737532542 118-119 0.030316349415649545 120-121 0.02344163820987028 122-123 0.01825742975961051 124-125 0.019046331045519605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 443655.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.74600253514362 #Duplication Level Percentage of deduplicated Percentage of total 1 70.76922778902347 22.46640084800495 2 10.788765696757977 6.8500036632069845 3 5.073332989499586 4.831741258388448 4 3.002558593805399 3.8127693092345387 5 1.9528140931578075 3.0997020576025975 6 1.385442746444102 2.6389361364546478 7 1.0274738588106653 2.2832731408618026 8 0.7597383415522181 1.929492425357002 9 0.5834838729989029 1.6670952460294732 >10 3.9424844407707047 24.186813911143858 >50 0.4456976350233182 9.935700091825264 >100 0.2611731364467852 14.3041276997166 >500 0.007097096099097425 1.3459686693250217 >1k 7.097096099097425E-4 0.6479755428488052 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2876 0.6482514566498744 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 750 0.16905027555194915 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 741 0.16702167224532577 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 665 0.14989124432272824 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 643 0.14493243623987107 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 543 0.12239239949961118 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 542 0.12216699913220859 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 534 0.1203637961929878 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 530 0.1194621947233774 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 524 0.11810979251896181 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 502 0.11315098443610463 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 484 0.10909377782285785 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 476 0.10729057488363707 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1270018370129943E-4 0.0 16-17 0.0 0.0 0.0 3.381005511038983E-4 0.0 18-19 0.0 0.0 0.0 6.762011022077966E-4 0.0 20-21 0.0 0.0 0.0 9.016014696103954E-4 0.0 22-23 0.0 0.0 0.0 0.0014651023881168926 0.0 24-25 0.0 0.0 0.0 0.0039445064295454794 0.0 26-27 0.0 0.0 0.0 0.007663612491688361 0.0 28-29 0.0 0.0 0.0 0.01803202939220791 0.0 30-31 0.0 0.0 0.0 0.043389570725000284 0.0 32-33 0.0 0.0 0.0 0.09647135724831232 0.0 34-35 0.0 0.0 0.0 0.17445988436961152 0.0 36-37 0.0 0.0 0.0 0.2874981686220148 0.0 38-39 0.0 0.0 0.0 0.44640542764084706 0.0 40-41 0.0 0.0 0.0 0.632698831299095 0.0 42-43 0.0 0.0 0.0 0.795663296931174 0.0 44-45 0.0 0.0 0.0 0.9707993824029933 0.0 46-47 0.0 0.0 0.0 1.1907901409879298 0.0 48-49 0.0 0.0 0.0 1.4229525194126067 0.0 50-51 0.0 0.0 0.0 1.6539878960002705 0.0 52-53 0.0 0.0 0.0 1.8752183566059213 0.0 54-55 0.0 0.0 0.0 2.100393323641118 0.0 56-57 0.0 0.0 0.0 2.3628720514814443 0.0 58-59 0.0 0.0 0.0 2.6611894377387832 0.0 60-61 0.0 0.0 0.0 2.9721292445706684 0.0 62-63 0.0 0.0 0.0 3.2796880458915147 0.0 64-65 0.0 0.0 0.0 3.6021232714609326 0.0 66-67 0.0 0.0 0.0 3.9679480677553505 0.0 68-69 0.0 0.0 0.0 4.354058897116003 0.0 70-71 0.0 0.0 0.0 4.803394529533083 0.0 72-73 0.0 0.0 0.0 5.246193551295488 0.0 74-75 0.0 0.0 0.0 5.679413057443283 0.0 76-77 0.0 0.0 0.0 6.158839638908612 0.0 78-79 0.0 0.0 0.0 6.702618025267381 0.0 80-81 0.0 0.0 0.0 7.305451307885631 0.0 82-83 0.0 0.0 0.0 7.891717663499792 0.0 84-85 0.0 0.0 0.0 8.488014335463367 0.0 86-87 0.0 0.0 0.0 9.142915102951617 0.0 88-89 0.0 0.0 0.0 9.848530953105454 0.0 90-91 0.0 2.2540036740259885E-4 0.0 10.614216001172082 0.0 92-93 0.0 2.2540036740259885E-4 0.0 11.452930768277152 0.0 94-95 0.0 2.2540036740259885E-4 0.0 12.269330899009365 0.0 96-97 0.0 2.2540036740259885E-4 0.0 13.111652071992879 0.0 98-99 0.0 2.2540036740259885E-4 0.0 14.012690040684767 0.0 100-101 0.0 2.2540036740259885E-4 0.0 14.98867363153802 0.0 102-103 0.0 2.2540036740259885E-4 0.0 16.01210399972952 0.0 104-105 0.0 2.2540036740259885E-4 0.0 17.04748058739336 0.0 106-107 0.0 2.2540036740259885E-4 0.0 18.08420957726161 0.0 108-109 0.0 2.2540036740259885E-4 0.0 19.131081583662983 0.0 110-111 0.0 2.2540036740259885E-4 0.0 20.257069119022663 0.0 112-113 0.0 2.2540036740259885E-4 0.0 21.466567490505007 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCATA 25 0.0020398034 71.54259 1 GCTTTCG 15 0.004238745 59.491093 72-73 TCCAACG 35 0.007728428 51.107624 2 GGTATCA 795 0.0 48.744953 1 GTATCAA 1410 0.0 43.551342 1 GCATATA 60 0.0012948771 39.745884 1 CGCTCGC 30 0.0013093139 39.66073 66-67 TAAGTCG 30 0.00131004 39.656258 42-43 GTACATG 2375 0.0 37.151943 1 TAGGGTC 195 1.8189894E-12 36.65537 4 TCAACGC 1540 0.0 36.35784 4 AACGCAG 1525 0.0 36.31257 6 CAACGCA 1625 0.0 35.17724 5 CTAGGGG 85 1.7001166E-4 35.03031 3 ATCAACG 1615 0.0 34.66157 3 ATAGGGT 275 0.0 34.648163 3 CGTCTTA 95 2.7284841E-11 34.504467 14-15 GGTGCTC 70 0.0027768791 34.025604 8 GACATAT 90 2.3800887E-4 33.08045 7 TATCAAC 1820 0.0 32.761303 2 >>END_MODULE