FastQCFastQC Report
Fri 27 May 2016
SRR940002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443655
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49021.1049126010075396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.8015237064836416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.5373544758877957No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8470.19091411119000123No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.17423448400220892No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7350.16566927004091014No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7280.16409146746909198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.1591326593862348No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6580.14831344175091005No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6320.1424530321984425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.13569102117636453No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT5700.12847820941948135No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5450.12284320023441637No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA5400.12171619839740339No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA5320.11991299545818258No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT5300.1194621947233774TruSeq Adapter, Index 10 (95% over 21bp)
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT5270.1187859936211696No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5170.1165319899471436No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5110.11517958774272803No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4960.11179858223168904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.083.263091
TACTCGG250.00205620671.398317
CGTCAGA453.969426E-666.154282
TATAACG551.7172897E-764.951482
GTATCAA34000.063.9383471
GCGTCAG507.400853E-659.5590061
ATCACGC401.766068E-459.5321433
GTACTCG300.004235236459.4985926
GTATCAC453.1533316E-452.9413381
TCACGCA453.160365E-452.917464
TATCAAC43850.049.287582
ATCAACG43850.049.282023
TCAACGC44250.048.7019964
CAACGCA45450.047.3894165
AACGCAG46300.046.6479236
GACATAT909.6306394E-846.323671
ACGCAGA52000.041.6490147
CGCAGAG52200.041.3754548
GCCATAT300.001308503139.6657350-51
GCAGAGT54300.039.446589