##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940002_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 443655 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.05261069975544 34.0 33.0 34.0 31.0 34.0 2 33.1070336184648 34.0 33.0 34.0 31.0 34.0 3 33.15976828842231 34.0 33.0 34.0 31.0 34.0 4 36.426624291397594 37.0 37.0 37.0 35.0 37.0 5 36.45059336646719 37.0 37.0 37.0 35.0 37.0 6 36.54472506790186 37.0 37.0 37.0 35.0 37.0 7 36.52255919577149 37.0 37.0 37.0 35.0 37.0 8 36.54061601920411 37.0 37.0 37.0 35.0 37.0 9 38.40134113218605 39.0 39.0 39.0 37.0 39.0 10-11 38.3866574252516 39.0 39.0 39.0 37.0 39.0 12-13 38.38675096640408 39.0 39.0 39.0 37.0 39.0 14-15 39.914026664863464 41.0 40.0 41.0 38.0 41.0 16-17 39.92678207165478 41.0 40.0 41.0 38.0 41.0 18-19 40.03165973560537 41.0 40.0 41.0 38.0 41.0 20-21 40.03375708602405 41.0 40.0 41.0 38.0 41.0 22-23 39.96442506001284 41.0 40.0 41.0 38.0 41.0 24-25 39.91998625057759 41.0 40.0 41.0 38.0 41.0 26-27 39.79701344513191 41.0 40.0 41.0 37.0 41.0 28-29 39.67003302115383 41.0 40.0 41.0 37.0 41.0 30-31 39.42057341853467 41.0 39.0 41.0 35.5 41.0 32-33 39.10838376666554 41.0 39.0 41.0 35.0 41.0 34-35 38.91929765245517 41.0 39.0 41.0 35.0 41.0 36-37 38.75409158016927 41.0 39.0 41.0 35.0 41.0 38-39 38.57109014887695 41.0 39.0 41.0 35.0 41.0 40-41 38.4072590188322 41.0 39.0 41.0 35.0 41.0 42-43 38.16160304741297 41.0 38.0 41.0 35.0 41.0 44-45 37.78040143805434 41.0 38.0 41.0 34.5 41.0 46-47 37.19516290811554 41.0 38.0 41.0 32.5 41.0 48-49 36.66812275304009 41.0 38.0 41.0 30.5 41.0 50-51 36.579161736033626 41.0 38.0 41.0 30.0 41.0 52-53 36.48377117354701 41.0 38.0 41.0 30.0 41.0 54-55 36.34309655024738 41.0 38.0 41.0 29.0 41.0 56-57 36.190739425905264 41.0 37.0 41.0 28.0 41.0 58-59 36.012972918145856 40.5 37.0 41.0 28.0 41.0 60-61 35.824583291070766 40.0 36.5 41.0 27.0 41.0 62-63 35.55628134473859 40.0 36.0 41.0 26.0 41.0 64-65 35.26925989789363 39.0 35.5 41.0 25.5 41.0 66-67 34.94195038937914 39.0 35.0 41.0 24.5 41.0 68-69 34.53636609527673 38.5 35.0 41.0 22.5 41.0 70-71 34.149442697591596 37.0 35.0 40.0 21.0 41.0 72-73 33.75592408515626 37.0 35.0 39.0 19.5 41.0 74-75 33.33348547858133 36.5 35.0 39.0 18.0 41.0 76-77 32.97073288929461 36.0 35.0 38.5 16.0 40.5 78-79 32.608657628111935 35.5 35.0 37.0 12.0 39.0 80-81 32.258241200933156 35.0 35.0 37.0 8.5 39.0 82-83 31.942068724572025 35.0 34.5 36.5 7.0 39.0 84-85 31.66496038588543 35.0 34.0 36.0 6.0 37.0 86-87 31.46017851709098 35.0 34.0 36.0 2.0 37.0 88-89 31.321722960408426 35.0 34.0 35.5 2.0 36.5 90-91 31.282072781778634 35.0 34.0 35.0 2.0 36.0 92-93 31.193862347995626 35.0 34.0 35.0 2.0 36.0 94-95 31.098563072657807 35.0 34.0 35.0 2.0 36.0 96-97 31.018699214479717 35.0 34.0 35.0 2.0 36.0 98-99 30.972332104901334 35.0 34.0 35.0 2.0 36.0 100-101 30.93010109206478 35.0 34.0 35.0 2.0 35.5 102-103 30.483544646177773 35.0 33.5 35.0 2.0 35.0 104-105 30.82257497379721 35.0 34.0 35.0 2.0 35.0 106-107 30.84203942252426 35.0 34.0 35.0 2.0 35.0 108-109 30.816200651407062 35.0 34.0 35.0 2.0 35.0 110-111 30.746329918517766 35.0 34.0 35.0 2.0 35.0 112-113 30.683655092357803 35.0 34.0 35.0 2.0 35.0 114-115 30.59315571784382 35.0 34.0 35.0 2.0 35.0 116-117 30.498763678984794 35.0 34.0 35.0 2.0 35.0 118-119 30.40724098680281 35.0 34.0 35.0 2.0 35.0 120-121 30.266762461822815 35.0 34.0 35.0 2.0 35.0 122-123 30.13189753299298 35.0 34.0 35.0 2.0 35.0 124-125 29.103121795088526 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22735069736470592 1101 2 0.1854865278559501 1101 3 0.15079110343704372 1101 4 0.1301846795966739 1101 5 0.1648787083801011 1101 6 0.14951321770021764 1101 7 0.16909019135482595 1101 8 0.1920844972066007 1101 9 0.19890441954424887 1101 10-11 0.1795836875073462 1101 12-13 0.2556748332314598 1101 14-15 0.37735447361064445 1101 16-17 0.30254282700473567 1101 18-19 0.26920123609634317 1101 20-21 0.2937318352806244 1101 22-23 0.30688886107041924 1101 24-25 0.23068934356409443 1101 26-27 0.32052388688324385 1101 28-29 0.3791335371831295 1101 30-31 0.4308562700729226 1101 32-33 0.2436205144665422 1101 34-35 0.32647610229246027 1101 36-37 0.33208895229589785 1101 38-39 0.46546661284452284 1101 40-41 0.44072363921439006 1101 42-43 0.2313208219600611 1101 44-45 0.17865991216684307 1101 46-47 0.4606474950780992 1101 48-49 0.41789327719598646 1101 50-51 0.49235218604032127 1101 52-53 0.3087576995543273 1101 54-55 0.2850414584484824 1101 56-57 0.2890601097763934 1101 58-59 0.27151253123248864 1101 60-61 0.29422595685885966 1101 62-63 0.39000610247179424 1101 64-65 0.3147669074999584 1101 66-67 0.3031948758407452 1101 68-69 0.12120471489833307 1101 70-71 0.30992367488489236 1101 72-73 0.1916232870069976 1101 74-75 0.1823103332363516 1101 76-77 0.22979674735437783 1101 78-79 -0.0062278166562208526 1101 80-81 0.12927279255544022 1101 82-83 0.10451779596293065 1101 84-85 0.2655238994090219 1101 86-87 0.144235697551931 1101 88-89 0.10408178886675756 1101 90-91 0.10322141085089243 1101 92-93 0.11601682607986774 1101 94-95 0.2214603184388686 1101 96-97 0.1718578322009101 1101 98-99 0.1972327690991058 1101 100-101 0.17903994449848426 1101 102-103 0.20984813430762017 1101 104-105 0.14487879330659226 1101 106-107 0.1824856596432305 1101 108-109 0.15872524590951897 1101 110-111 0.1844665810905859 1101 112-113 0.2474897241266767 1101 114-115 0.1576137062208538 1101 116-117 0.0646629826741183 1101 118-119 0.08662724944628408 1101 120-121 0.1821792435632723 1101 122-123 0.05558472839494044 1101 124-125 0.003541896113461007 1102 1 0.09231683384135891 1102 2 0.1087385467102635 1102 3 0.17348701810236378 1102 4 -0.01795685382210621 1102 5 0.11823319328334492 1102 6 0.12504533376770866 1102 7 0.07836606525785328 1102 8 0.0870900652523261 1102 9 0.2134233614054466 1102 10-11 0.2274426163414205 1102 12-13 0.2243046737542258 1102 14-15 0.3085260744763261 1102 16-17 0.35904273245301965 1102 18-19 0.2828201856617554 1102 20-21 0.1462083899560298 1102 22-23 0.16497198253785683 1102 24-25 0.11446601940577494 1102 26-27 0.06384923584912627 1102 28-29 0.17078356975094522 1102 30-31 0.14361514917979434 1102 32-33 0.1670826482537251 1102 34-35 0.09706036188246259 1102 36-37 -0.12889795754377786 1102 38-39 -0.1658394615781873 1102 40-41 -0.23054979735541536 1102 42-43 -0.2444756887312849 1102 44-45 -0.25589804103356073 1102 46-47 -0.12370489567965137 1102 48-49 0.26058372887305126 1102 50-51 0.3061816007301559 1102 52-53 0.03456996886564667 1102 54-55 -0.0537285987728211 1102 56-57 -0.15606487358951426 1102 58-59 -0.2699133787402701 1102 60-61 -0.24226575410703788 1102 62-63 -0.4672365592641725 1102 64-65 -0.4737769259769493 1102 66-67 -0.5046129950646403 1102 68-69 -0.6045760371730395 1102 70-71 -0.588205601829479 1102 72-73 -0.531627331081097 1102 74-75 -0.3546716459062367 1102 76-77 -0.44825464675820115 1102 78-79 -0.3365337439984728 1102 80-81 -0.49738974185088125 1102 82-83 -0.329005341193092 1102 84-85 -0.29280050403966484 1102 86-87 -0.226674940567122 1102 88-89 -0.2678111365634521 1102 90-91 -0.18799438418899683 1102 92-93 -0.37472060729619017 1102 94-95 -0.2575737131764271 1102 96-97 -0.19658920833025917 1102 98-99 -0.18367233599338206 1102 100-101 -0.1994488388660116 1102 102-103 -0.24027233158722439 1102 104-105 -0.2819504322798032 1102 106-107 -0.18922866809404226 1102 108-109 -0.24457636006894745 1102 110-111 -0.25668197717078556 1102 112-113 -0.17324091553321708 1102 114-115 -0.22182409625710164 1102 116-117 -0.2521780793868338 1102 118-119 -0.26402480722871857 1102 120-121 -0.08512897403175401 1102 122-123 -0.18213230282113457 1102 124-125 -0.29332423966280885 1103 1 0.22230767633219983 1103 2 0.17279532326703873 1103 3 0.18072145033679732 1103 4 0.11459809123283549 1103 5 0.13581561086576244 1103 6 0.10787500409737305 1103 7 0.19123236562415258 1103 8 0.19588127404353628 1103 9 0.18347830646038688 1103 10-11 0.2724288800776833 1103 12-13 0.27293104738059526 1103 14-15 0.34897937117961675 1103 16-17 0.31130921596950145 1103 18-19 0.28497220031377424 1103 20-21 0.3170417232893641 1103 22-23 0.33200494957082327 1103 24-25 0.37735521354497337 1103 26-27 0.4445268915267846 1103 28-29 0.38658027304764886 1103 30-31 0.4560235740882206 1103 32-33 0.5073070072280785 1103 34-35 0.5947480908201825 1103 36-37 0.6614866578408325 1103 38-39 0.6588171318284068 1103 40-41 0.5076828033771861 1103 42-43 0.5023191830635838 1103 44-45 0.567911482775969 1103 46-47 0.775974591499839 1103 48-49 0.8758538753931973 1103 50-51 0.77154332234187 1103 52-53 0.7377064157520934 1103 54-55 0.6729426466484227 1103 56-57 0.694850877326239 1103 58-59 0.6542853392084496 1103 60-61 0.5572534766621899 1103 62-63 0.6168293748017604 1103 64-65 0.605321059371029 1103 66-67 0.708642499440856 1103 68-69 0.6975072961602891 1103 70-71 0.8658237021998261 1103 72-73 0.7310558191020533 1103 74-75 0.7439089401743431 1103 76-77 0.8914431554396032 1103 78-79 0.7947162560015286 1103 80-81 0.6995882801271378 1103 82-83 0.6774964903087373 1103 84-85 0.7047498622607051 1103 86-87 0.7139844000922189 1103 88-89 0.8321888634365493 1103 90-91 0.69183620189159 1103 92-93 0.7117949604693798 1103 94-95 0.6267440523913415 1103 96-97 0.6595234290323795 1103 98-99 0.6970740010029566 1103 100-101 0.7219568387896658 1103 102-103 0.7511653973505013 1103 104-105 0.7767263076469355 1103 106-107 0.7335048483894724 1103 108-109 0.8187020282094366 1103 110-111 0.7584508067119984 1103 112-113 0.7106418683495654 1103 114-115 0.6707812150982093 1103 116-117 0.6219748510160983 1103 118-119 0.7501464381925267 1103 120-121 0.7873298171770386 1103 122-123 0.6811002979114669 1103 124-125 0.6761125735240014 1104 1 0.202312164662537 1104 2 0.21025043996362314 1104 3 0.19164091686667462 1104 4 0.09957821434132796 1104 5 0.16777252289448796 1104 6 0.14559300871394498 1104 7 0.2329257485428471 1104 8 0.18200592138900618 1104 9 0.17591867578585862 1104 10-11 0.22579031890815315 1104 12-13 0.21103697197664673 1104 14-15 0.231094977745407 1104 16-17 0.260615127741751 1104 18-19 0.3200420900290837 1104 20-21 0.2050482634278623 1104 22-23 0.20978130248182936 1104 24-25 0.17171113814102767 1104 26-27 0.21882158247318273 1104 28-29 0.2882890445275166 1104 30-31 0.32884673387277985 1104 32-33 0.3885858607802746 1104 34-35 0.25104297412359955 1104 36-37 0.433075526781586 1104 38-39 0.44930347448036656 1104 40-41 0.49938259819636954 1104 42-43 0.6299480622633808 1104 44-45 0.565231308372276 1104 46-47 0.6940080616203801 1104 48-49 0.7450110310741422 1104 50-51 0.6360903215724463 1104 52-53 0.5602586727410639 1104 54-55 0.6263553806827957 1104 56-57 0.4868744731200323 1104 58-59 0.42809432869293573 1104 60-61 0.6042382804580342 1104 62-63 0.6680989310982497 1104 64-65 0.5938998616280173 1104 66-67 0.7112313042626042 1104 68-69 0.7318991273990676 1104 70-71 0.8209788701916239 1104 72-73 0.8359192456216675 1104 74-75 0.9147251751063834 1104 76-77 0.7244227655960529 1104 78-79 0.6908450710823217 1104 80-81 0.7880532711504813 1104 82-83 0.7220399629940424 1104 84-85 0.7604708804704998 1104 86-87 0.7874263082737976 1104 88-89 0.723212202754322 1104 90-91 0.7417640680111077 1104 92-93 0.7344658376173641 1104 94-95 0.7552369351817987 1104 96-97 0.6773725570139106 1104 98-99 0.7086587022083926 1104 100-101 0.688581293778121 1104 102-103 0.6832665771401913 1104 104-105 0.5670427349359848 1104 106-107 0.5838591058913956 1104 108-109 0.5661180323130566 1104 110-111 0.6425508964786069 1104 112-113 0.606794728057185 1104 114-115 0.5743350109432974 1104 116-117 0.7061166181358729 1104 118-119 0.6477852558380768 1104 120-121 0.6665825101752425 1104 122-123 0.5464535936283177 1104 124-125 0.6613732172788147 1105 1 0.20722146943564468 1105 2 0.19908842671531346 1105 3 0.22517957841561298 1105 4 0.18030006167618495 1105 5 0.18926388672782934 1105 6 0.1451286986786542 1105 7 0.07257473015616966 1105 8 0.15080738241791636 1105 9 0.2508428384800965 1105 10-11 0.1888389785998612 1105 12-13 0.1582758749668045 1105 14-15 0.21692887856877974 1105 16-17 0.17753458542762957 1105 18-19 0.19377761903298563 1105 20-21 0.1541722651612929 1105 22-23 0.14555174760037914 1105 24-25 0.1827431445794545 1105 26-27 0.11053674374353051 1105 28-29 0.09747928782596915 1105 30-31 0.04266150512269462 1105 32-33 0.06509641609507355 1105 34-35 0.02427395288915335 1105 36-37 0.008038381978764164 1105 38-39 0.025473535769293676 1105 40-41 0.0711680250520601 1105 42-43 0.04554578404880516 1105 44-45 -0.05918211328314271 1105 46-47 0.1165534092338234 1105 48-49 0.07877875224048836 1105 50-51 -0.02159091411132863 1105 52-53 0.03010173594913823 1105 54-55 -0.1078147425141367 1105 56-57 -0.12362202907770126 1105 58-59 -0.08176761645657393 1105 60-61 -0.10792509476638656 1105 62-63 -0.028367669533217565 1105 64-65 -0.029315640136360344 1105 66-67 -0.06540306706160948 1105 68-69 -0.17210477280522696 1105 70-71 -0.2826984652878579 1105 72-73 -0.3066904863166684 1105 74-75 -0.3284432765744185 1105 76-77 -0.28608147510226445 1105 78-79 -0.29084408882606283 1105 80-81 -0.19782551297630846 1105 82-83 -0.2390059727454492 1105 84-85 -0.27440654168018597 1105 86-87 -0.3631091959668957 1105 88-89 -0.3169490675979354 1105 90-91 -0.3094261133793452 1105 92-93 -0.35202893115623723 1105 94-95 -0.44274486386481726 1105 96-97 -0.31969455126318636 1105 98-99 -0.4374395457950726 1105 100-101 -0.45258133855023885 1105 102-103 -0.5290685927972767 1105 104-105 -0.3678857613185862 1105 106-107 -0.3638781565366358 1105 108-109 -0.359007331396473 1105 110-111 -0.39435091742593187 1105 112-113 -0.5155896587440303 1105 114-115 -0.5123160755421843 1105 116-117 -0.43482933617601915 1105 118-119 -0.36835725638875516 1105 120-121 -0.2861615621333051 1105 122-123 -0.24334551489641498 1105 124-125 -0.18641821465333663 1106 1 0.18650835660430687 1106 2 0.1948191327908475 1106 3 0.14143573605107918 1106 4 0.042744349736238973 1106 5 0.12255030474331363 1106 6 0.14741582042390888 1106 7 0.15424256970578654 1106 8 0.17853433526802576 1106 9 0.20269599353522239 1106 10-11 0.2828455467443547 1106 12-13 0.2679990745914864 1106 14-15 0.317814405193225 1106 16-17 0.2753823452212032 1106 18-19 0.27536335677636004 1106 20-21 0.16746689335739973 1106 22-23 0.20088250059123425 1106 24-25 0.18275487340891772 1106 26-27 0.18806430649276962 1106 28-29 0.19891020502043943 1106 30-31 0.15100656728549922 1106 32-33 0.1782678915818252 1106 34-35 -0.09492027652675716 1106 36-37 -0.03477184556052748 1106 38-39 -0.1200519157906399 1106 40-41 -0.17998726465002335 1106 42-43 -0.14478966047382613 1106 44-45 -0.1453538233464826 1106 46-47 -0.3457090819695523 1106 48-49 -0.649018573586396 1106 50-51 -0.6414243647329556 1106 52-53 -0.5959245366288641 1106 54-55 -0.5319468091338848 1106 56-57 -0.5378021838982576 1106 58-59 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-0.648155458706654 2216 102-103 -0.8132491829145927 2216 104-105 -0.5384154449303828 2216 106-107 -0.5563239439522043 2216 108-109 -0.5755910498465262 2216 110-111 -0.5181600180302688 2216 112-113 -0.4410517988080187 2216 114-115 -0.44161753306084606 2216 116-117 -0.4879754435347117 2216 118-119 -0.49754317889142285 2216 120-121 -0.5671160591116227 2216 122-123 -0.5873643559913297 2216 124-125 -0.658929768154934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 2.0 4 10.0 5 35.0 6 138.0 7 346.0 8 566.0 9 882.0 10 1848.0 11 2552.0 12 2949.0 13 4742.0 14 9173.0 15 9202.0 16 2851.0 17 1708.0 18 1437.0 19 1560.0 20 1583.0 21 1853.0 22 1338.0 23 1056.0 24 1129.0 25 1238.0 26 1514.0 27 1823.0 28 2248.0 29 2737.0 30 3483.0 31 4363.0 32 5665.0 33 7801.0 34 10625.0 35 18100.0 36 36961.0 37 165018.0 38 134788.0 39 324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61532270609161 22.664836093846162 14.934723847268907 20.785117352793314 2 14.917083484544866 22.32378127261129 41.98210707354403 20.777028169299815 3 16.180627245556224 29.709863993111753 33.21146659333625 20.898042167995783 4 10.787307562964518 19.492762945559026 41.96363078270329 27.756298708773166 5 10.271368523596479 39.89054156443931 36.53936354452302 13.298726367441189 6 25.276437078299608 42.39693771965617 18.54072783262059 13.785897369423635 7 23.181751586255086 36.70735143298284 24.099581882318468 16.01131509844361 8 23.93909682072782 35.578771793398026 21.814247557223517 18.66788382865064 9 26.024050219201854 15.964431822023869 23.722036266919115 34.28948169185516 10-11 21.84884651361982 28.496917649975767 30.349257869290327 19.304977967114088 12-13 23.982711791820222 27.546066200087903 30.054546888911425 18.416675119180447 14-15 21.59831400525183 27.854752003245764 29.510993902920063 21.035940088582343 16-17 18.055696430785183 30.903742773100717 30.542651384521758 20.497909411592342 18-19 17.58618746548557 27.921245111629535 35.993508469418806 18.499058953466093 20-21 18.741927849342392 27.213262557617966 36.336342428238154 17.708467164801476 22-23 19.039343634130123 27.212473656332058 35.60086102940348 18.147321680134336 24-25 18.542899324925898 27.870980829698755 35.854549142915104 17.731570702460246 26-27 18.02515468100213 27.544037596781283 36.42131836674894 18.00948935546765 28-29 18.19180756131481 26.822313835362323 36.782124768681825 18.203753834641038 30-31 18.99203096102936 26.630069119100565 36.127324303033106 18.250575616836976 32-33 18.558852798411372 27.117559086088583 36.53064835507891 17.792939760421138 34-35 18.679463454546685 26.947749705287695 36.18278248991892 18.190004350246703 36-37 19.500213006227895 26.329598398734134 35.0537249041472 19.11646369089077 38-39 18.578472531373798 27.551801281437193 35.24042444085046 18.62930174633855 40-41 18.536813515006028 26.816896011540496 36.36880008114413 18.27749039230934 42-43 18.675144850033305 26.779759201259555 35.49566830497567 19.049427643731466 44-45 18.895729859427636 26.89481923270683 34.96840418302244 19.24104672484309 46-47 19.765853570575267 27.033679398810783 33.44362949505696 19.756837535556986 48-49 19.387329594633584 27.574901723889212 32.91393176572418 20.123836915753028 50-51 19.314585568609065 27.70983040455861 32.293911334313094 20.681672692519225 52-53 19.393898412054412 28.179215832121805 32.56280217736755 19.86408357845623 54-55 19.888877618870517 27.9790603058683 31.850424316191635 20.281637759069547 56-57 19.684751848583513 28.55998133666028 31.776702607119795 19.978564207636417 58-59 18.934465767751764 27.857796531468782 32.438588424103905 20.76914927667555 60-61 19.84413388932717 28.909838836920997 31.21514707539727 20.03088019835456 62-63 19.42099023112606 28.59232645259594 31.677275534538175 20.309407781739825 64-65 19.390917635709148 28.185217659827206 31.921713238559136 20.502151465904504 66-67 19.32924829511452 28.578287236716204 31.28638131506374 20.806083153105533 68-69 19.47334610616477 27.877155415304856 31.618280175814267 21.031218302716105 70-71 19.54466409933044 28.042118739863923 32.009430847029364 20.403786313776273 72-73 19.86814078506948 27.35751879275563 31.70447757829845 21.069862843876436 74-75 19.392386175247125 28.44629874440301 30.77469241198368 21.386622668366194 76-77 19.387813754185547 28.392985746309783 31.33354897388533 20.885651525619338 78-79 19.36400387241759 27.87002381379036 31.243287227220527 21.522685086571524 80-81 19.603182653187726 27.919554608874016 30.90013636722228 21.577126370715984 82-83 19.742818180793634 28.21888629678466 30.666959687144292 21.37133583527741 84-85 19.292893319779196 27.713100103008433 30.59327898154639 22.40072759566598 86-87 19.41246875373321 27.78319993328119 31.080145969935963 21.724185343049633 88-89 19.92697028096156 27.496365419075637 30.796114097665978 21.78055020229683 90-91 19.285862406289585 27.383839658833235 31.218359352107722 22.111938582769454 92-93 19.803742130638724 27.362273498763667 31.030810254005964 21.803174116591645 94-95 19.705692086797956 26.794372152885938 31.160867438594753 22.339068321721353 96-97 20.12315931595188 26.685269794185995 30.663153410435633 22.528417479426487 98-99 19.44923420225175 26.92508818789375 30.559894512628055 23.065783097226447 100-101 19.598740909290697 27.020073097586295 30.728823282133465 22.652362710989546 102-103 20.016341526636687 26.499081493502835 30.538932278459612 22.945644701400862 104-105 19.465012227969932 26.47980976208991 30.89145845307728 23.163719556862876 106-107 19.436902898320422 26.829496442606676 30.353643102105583 23.37995755696732 108-109 19.556093514736776 27.084629394209873 29.841632630981042 23.517644460072308 110-111 19.451262805558372 26.590594042668293 30.265183532249157 23.692959619524178 112-113 19.207873703381466 26.48415206444662 30.980786829376044 23.327187402795875 114-115 18.88371338184761 27.274120674090696 30.01096586381817 23.831200080243526 116-117 18.87998791832245 27.431381255818255 29.72160298615791 23.967027839701384 118-119 19.478060556335695 26.518810872461135 29.967113938145353 24.036014633057817 120-121 19.001046985886543 26.955209515512635 29.97557784266811 24.068165655932713 122-123 19.101167915952427 27.284919424731235 29.600803329625485 24.013109329690852 124-125 19.08851880361049 26.96835241174661 29.4871086877876 24.4560200968553 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 475.0 1 312.5 2 288.0 3 386.0 4 220.5 5 140.5 6 319.0 7 767.0 8 1328.0 9 1419.0 10 919.0 11 506.5 12 481.5 13 533.0 14 577.5 15 657.5 16 743.0 17 808.5 18 868.0 19 930.0 20 1040.0 21 1178.5 22 1334.5 23 1511.5 24 1637.0 25 1978.5 26 3167.0 27 4850.0 28 5337.5 29 5974.0 30 8058.5 31 9871.5 32 12298.0 33 15332.5 34 18516.0 35 20356.0 36 21908.5 37 25222.5 38 25106.0 39 22412.0 40 20003.0 41 18227.0 42 17297.0 43 16606.5 44 16081.5 45 15653.5 46 14121.0 47 11771.5 48 11215.0 49 10504.0 50 9199.5 51 7930.0 52 6914.5 53 6821.0 54 6533.0 55 5837.0 56 5236.0 57 4649.5 58 3991.0 59 3426.5 60 2987.5 61 2368.0 62 1660.0 63 1169.5 64 897.0 65 706.5 66 583.5 67 486.5 68 317.0 69 198.0 70 148.5 71 128.0 72 109.5 73 86.5 74 74.0 75 62.5 76 42.0 77 30.0 78 21.0 79 10.0 80 5.0 81 5.0 82 4.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017581228657402713 2 0.0036064058784415817 3 2.2540036740259885E-4 4 0.13253541603272814 5 0.04485467311311717 6 0.014425623513766327 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.508007348051977E-4 30-31 1.1270018370129943E-4 32-33 3.3810055110389833E-4 34-35 4.508007348051977E-4 36-37 0.0013524022044155933 38-39 0.0028175045925324855 40-41 0.0 42-43 0.0014651023881168926 44-45 0.001014301653311695 46-47 2.2540036740259885E-4 48-49 0.001577802571818192 50-51 0.0024794040414285873 52-53 0.0 54-55 0.0 56-57 0.001014301653311695 58-59 0.00202860330662339 60-61 0.0011270018370129944 62-63 2.2540036740259885E-4 64-65 9.016014696103954E-4 66-67 7.88901285909096E-4 68-69 0.0011270018370129944 70-71 0.001014301653311695 72-73 0.0 74-75 3.3810055110389833E-4 76-77 0.0034937056947402824 78-79 0.001014301653311695 80-81 0.0 82-83 0.0 84-85 4.508007348051977E-4 86-87 4.508007348051977E-4 88-89 0.0 90-91 2.2540036740259885E-4 92-93 9.016014696103954E-4 94-95 9.016014696103954E-4 96-97 4.508007348051977E-4 98-99 0.0 100-101 3.3810055110389833E-4 102-103 0.0 104-105 0.0 106-107 3.3810055110389833E-4 108-109 6.762011022077967E-4 110-111 0.0 112-113 2.2540036740259885E-4 114-115 0.0012397020207142936 116-117 0.0018032029392207908 118-119 4.508007348051977E-4 120-121 1.1270018370129943E-4 122-123 3.3810055110389833E-4 124-125 7.88901285909096E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 443655.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.070209109794 #Duplication Level Percentage of deduplicated Percentage of total 1 64.31265316868543 16.76644316513285 2 12.77006648798408 6.658366073754353 3 6.1814598238584 4.834558506353367 4 3.6471060633268624 3.8032327086611564 5 2.44548026340599 3.1877090920434137 6 1.7117248742405198 2.677501524593171 7 1.275079878496656 2.326911934486898 8 0.9837458490847386 2.0517167997224792 9 0.7823269249469205 1.83558838730295 >10 4.977498351395127 25.18980286853742 >50 0.5549763617020342 10.282895088787637 >100 0.3414574188042111 15.69883562472488 >500 0.013831186584474374 2.2430449464956466 >1k 0.002593347484588945 2.4433932794037774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4902 1.1049126010075396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3556 0.8015237064836416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2384 0.5373544758877957 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 847 0.19091411119000123 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 773 0.17423448400220892 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 735 0.16566927004091014 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 728 0.16409146746909198 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 706 0.1591326593862348 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 658 0.14831344175091005 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 632 0.1424530321984425 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 602 0.13569102117636453 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 570 0.12847820941948135 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 545 0.12284320023441637 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 540 0.12171619839740339 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 532 0.11991299545818258 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 530 0.1194621947233774 TruSeq Adapter, Index 10 (95% over 21bp) ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 527 0.1187859936211696 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 517 0.1165319899471436 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 511 0.11517958774272803 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 496 0.11179858223168904 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1270018370129943E-4 0.0 16-17 0.0 0.0 0.0 2.2540036740259885E-4 0.0 18-19 0.0 0.0 0.0 4.508007348051977E-4 0.0 20-21 0.0 0.0 0.0 6.762011022077966E-4 0.0 22-23 0.0 0.0 0.0 0.0012397020207142936 0.0 24-25 0.0 0.0 0.0 0.0037191060621428814 0.0 26-27 0.0 0.0 0.0 0.007438212124285763 0.0 28-29 0.0 0.0 0.0 0.017468528473701412 0.0 30-31 0.0 0.0 0.0 0.042713369622792485 0.0 32-33 0.0 0.0 0.0 0.09635865706461103 0.0 34-35 0.0 0.0 0.0 0.17536148583922193 0.0 36-37 0.0 0.0 0.0 0.28963947211233954 0.0 38-39 0.0 0.0 0.0 0.45046263425409383 0.0 40-41 0.0 0.0 0.0 0.6390100415863678 0.0 42-43 0.0 0.0 0.0 0.8034396096065637 0.0 44-45 0.0 0.0 0.0 0.980491598201305 0.0 46-47 0.0 0.0 0.0 1.2019474591743584 0.0 48-49 0.0 0.0 0.0 1.4387305451307886 0.0 50-51 0.0 0.0 0.0 1.6729215268620887 0.0 52-53 0.0 0.0 0.0 1.8983218942646878 0.0 54-55 0.0 0.0 0.0 2.126088965525014 0.0 56-57 0.0 0.0 0.0 2.392286799427483 0.0 58-59 0.0 0.0 0.0 2.6931962899099524 0.0 60-61 0.0 0.0 0.0 3.0071790017017728 0.0 62-63 0.0 0.0 0.0 3.3183442089010606 0.0 64-65 0.0 0.0 0.0 3.642920737960803 0.0 66-67 0.0 0.0 0.0 4.010661437378143 0.0 68-69 0.0 0.0 0.0 4.398462769494314 0.0 70-71 0.0 0.0 0.0 4.850728606687628 0.0 72-73 0.0 0.0 0.0 5.297922935614385 0.0 74-75 0.0 0.0 0.0 5.7354250487428295 0.0 76-77 0.0 0.0 0.0 6.2185707362702995 0.0 78-79 0.0 0.0 0.0 6.767307930711928 0.0 80-81 0.0 0.0 0.0 7.373747619208619 0.0 82-83 0.0 0.0 0.0 7.963733080884922 0.0 84-85 0.0 0.0 0.0 8.565777462217262 0.0 86-87 0.0 0.0 0.0 9.226989439992789 0.0 88-89 0.0 0.0 0.0 9.94105780392422 0.0 90-91 0.0 0.0 0.0 10.71384296356403 0.0 92-93 0.0 0.0 0.0 11.563376948304427 0.0 94-95 0.0 0.0 0.0 12.388905793916443 0.0 96-97 0.0 0.0 0.0 13.238777879207944 0.0 98-99 0.0 0.0 0.0 14.14646515873821 0.0 100-101 0.0 0.0 0.0 15.127971058592825 0.0 102-103 0.0 0.0 0.0 16.16064284184783 0.0 104-105 0.0 0.0 0.0 17.204133842738166 0.0 106-107 0.0 0.0 0.0 18.255288456120184 0.0 108-109 0.0 0.0 0.0 19.31128917740136 0.0 110-111 0.0 0.0 0.0 20.44820863058007 0.0 112-113 0.0 0.0 0.0 21.669089720616245 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 83.26309 1 TACTCGG 25 0.002056206 71.39831 7 CGTCAGA 45 3.969426E-6 66.15428 2 TATAACG 55 1.7172897E-7 64.95148 2 GTATCAA 3400 0.0 63.938347 1 GCGTCAG 50 7.400853E-6 59.559006 1 ATCACGC 40 1.766068E-4 59.532143 3 GTACTCG 30 0.0042352364 59.498592 6 GTATCAC 45 3.1533316E-4 52.941338 1 TCACGCA 45 3.160365E-4 52.91746 4 TATCAAC 4385 0.0 49.28758 2 ATCAACG 4385 0.0 49.28202 3 TCAACGC 4425 0.0 48.701996 4 CAACGCA 4545 0.0 47.389416 5 AACGCAG 4630 0.0 46.647923 6 GACATAT 90 9.6306394E-8 46.32367 1 ACGCAGA 5200 0.0 41.649014 7 CGCAGAG 5220 0.0 41.375454 8 GCCATAT 30 0.0013085031 39.66573 50-51 GCAGAGT 5430 0.0 39.44658 9 >>END_MODULE