FastQCFastQC Report
Fri 27 May 2016
SRR940001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803137
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17690.220261300375901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.052.9597131
GGTATCA9900.043.396631
ATCAACG19450.041.6893733
CAACGCA19450.041.6763655
TCAACGC19600.041.375494
AACGCAG20750.039.0653156
TATCAAC22250.038.081762
GTACATG46350.035.274391
TACATGG45750.035.084872
ACATGGG46600.032.113963
ACGCAGA25900.031.7577577
CGCAGAG27300.030.1291548
CATGGGG23500.029.1803364
GTCTACG850.007109161528.0801721
TAGACGG850.007148284528.0486245
GCAGAGT31150.025.4390859
CATGGGA28200.024.5283994
ATACGTC1501.666255E-423.8413315
ATGGGGA14750.023.4372395
GATATAG1953.948983E-521.420131
Produced by FastQC (version 0.11.5)