FastQCFastQC Report
Fri 27 May 2016
SRR940001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803137
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.5137355146133225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.3420337999618994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.25537361620744653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32200.058.2949451
GGTATCA17900.056.5942231
TAACGCA1051.10958354E-1051.0742954
ACTATCG350.00779854750.9947978
ATAACGC1004.090907E-947.6693423
ATCAACG41800.044.3336263
TATCAAC42500.043.743632
CAACGCA42850.043.3430865
TCAACGC42800.043.1585734
TATAACG1151.4069883E-841.45162
AACGCAG45050.041.3405536
ACGCAGA52600.035.6284947
GTACATG43100.035.5330161
CGCAGAG54150.034.6086588
TACATGG43750.034.1857262
ACATGGG44050.033.1412853
GCAGAGT58650.031.5475069
GTATAAC2500.030.9870031
CATGGGG25850.028.5831644
TATCGTG850.007208305428.000636
Produced by FastQC (version 0.11.5)