FastQCFastQC Report
Fri 27 May 2016
SRR940000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371270
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17400.047.3077471
GGTATCA11500.042.5322531
TCAACGC19000.042.3511284
ATCAACG19900.041.0272983
CAACGCA20000.039.904915
AACGCAG21100.037.542296
GTACATG52350.036.1197661
TATCAAC22850.036.020332
TACATGG53950.033.8286552
ACATGGG54000.033.2183573
ACGCAGA27450.028.6406657
CATGGGG39650.028.4121044
CGCAGAG28250.027.4079468
ATGGGGA21300.027.4029965
GCAGAGT34400.022.1576549
CATGGGA22200.021.7478774
GAGTACA37900.021.4043521
TGGGGAG22150.020.435796
AGTACAT36750.019.961932
GTGTAGG8050.019.2654821
Produced by FastQC (version 0.11.5)