FastQCFastQC Report
Fri 27 May 2016
SRR940000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371270
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.2006898714330511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.142203942330832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.066.832391
GTATCAA34400.053.7234951
TCAACGC41650.043.4971664
ATCAACG42500.042.6272243
CAACGCA43000.042.07475
ATAACGC853.4438071E-642.0663383
TATCAAC45250.040.302962
AACGCAG45400.039.9713676
GTACATG49550.035.3724141
TATAACG1209.1709444E-734.7656942
ACGCAGA52900.034.414317
TACATGG51100.034.406082
CGCAGAG54950.033.130438
ACATGGG53000.031.8209363
GCAGAGT61600.029.2641039
TAACGCA1254.8310285E-528.605114
CATGGGG39450.026.1337344
ACGACCG1200.00128628124.7973145
GAGTACT41350.024.53139112-13
CAGAGTA61950.024.44104610-11
Produced by FastQC (version 0.11.5)