FastQCFastQC Report
Fri 27 May 2016
SRR939999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345481
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22050.048.687731
GGTATCA15050.045.573981
ATCAACG25500.040.431673
TCAACGC25800.039.9645164
CAACGCA26650.038.4461565
GTACATG66100.037.2652661
AACGCAG27950.037.0842176
TACATGG65900.036.0207942
TATCAAC29700.035.1428682
ACATGGG67700.034.3314173
ACGCAGA33900.030.3996167
TCGACGC400.00537201629.74030960-61
CGCAGAG34800.029.4422448
CATGGGG38450.028.9863414
CGTCGAT450.0095446126.43681344-45
CATGGGA33800.025.744494
TAAGGTG6250.023.8276775
GCAGAGT43200.023.709429
ATGGGGA21200.022.7599285
GTATTGG6050.022.6739941
Produced by FastQC (version 0.11.5)