FastQCFastQC Report
Fri 27 May 2016
SRR939999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345481
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.3139397732112159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.20944182786676288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.14470661421454484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.060.6743551
GTATCAA48250.057.433681
ATCAACG58650.046.9322973
TCAACGC59850.046.0908474
CAACGCA60750.045.262785
TATCAAC62600.043.972552
AACGCAG63550.043.255656
ACGCAGA74600.036.845747
CGCAGAG76100.035.963128
GTACATG64950.035.692871
TACATGG66350.034.0339472
ACATGGG64500.033.9927253
GCAGAGT85000.031.9875839
TAACGCA1701.24437065E-831.5421624
TATAACG1602.5723602E-729.790932
ATAACGC1802.1706E-829.789823
CATGGGG37650.029.5920124
GAGTACT48600.027.17695612-13
GGGTACG900.00945557726.486741
GTGGTAT13050.026.486741
Produced by FastQC (version 0.11.5)