FastQCFastQC Report
Fri 27 May 2016
SRR939998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772857
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40890.529075883378167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.35918675770550046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.2564510640390137No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT17170.22216270280271772No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA17150.22190392271791548No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT14020.1814048394463659No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13130.16988912567266648No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC13060.16898339537585866No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA13030.1685952252486553No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG12990.16807766507905084No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG12570.16264328329820393No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC12490.161608162958995No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT12310.1592791421957749No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT11790.15255085999091683No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG11710.15151573965170786No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC11660.15086878943970228No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA11640.15061000935490007No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT11560.14957488901569113No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA11110.14375233710764088No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA10510.13598893456357386No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT10300.1332717436731504No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG10250.1326247934611448No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT10170.1315896731219359No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9800.12680224155309455No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9760.12628468138349008No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT9750.12615529134108897No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9420.1218854199418521No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9350.1209796896450443No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG9170.1186506688818242No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG9130.11813310871221974No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA8860.11463957756738957No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG8720.11282811697377393No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT8610.11140482650736165No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8600.11127543646496053No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8590.1111460464225594No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT8520.11024031612575158No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC8440.10920519578654267No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA8400.10868763561693819No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT8270.10700556506572367No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG8230.10648800489611922No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC8190.10597044472651473No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC8170.1057116646417125No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG8140.10532349451450915No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7830.10131240320007454No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT7820.10118301315767342No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC7760.10040667290326671No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC7750.1002772828608656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.068.4777451
GTATGGT1400.059.6419031
GTATCAA33850.051.625051
GGATGCC1500.051.5625728
GGGATGC1650.046.875077
GGGCCTA951.4876059E-743.8386277
TCAACGC40050.043.0207334
CAACGCA40250.042.888365
ATCAACG40600.042.4379463
AACGCAG41350.041.872426
GGTACTC2000.041.6466948
GTGGTAC2100.039.6660846
TGGTACT2100.039.6635177
ACATATA3400.038.5843122
GACATAT3200.037.276191
GTCTTTA801.1804392E-437.276191
ACGCAGA46550.036.809117
CGCAGAG46650.036.73028
GTATTGA650.001915966736.702711
GGATGAC3600.036.3582278
Produced by FastQC (version 0.11.5)