##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939998_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 772857 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.10220001889095 34.0 33.0 34.0 31.0 34.0 2 33.17147415369208 34.0 33.0 34.0 31.0 34.0 3 33.2287952363762 34.0 33.0 34.0 31.0 34.0 4 36.46927051187995 37.0 37.0 37.0 35.0 37.0 5 36.48512596767578 37.0 37.0 37.0 35.0 37.0 6 36.572129126086715 37.0 37.0 37.0 35.0 37.0 7 36.551587162955116 37.0 37.0 37.0 35.0 37.0 8 36.56406553864428 37.0 37.0 37.0 35.0 37.0 9 38.447918567082915 39.0 39.0 39.0 37.0 39.0 10-11 38.42758880362085 39.0 39.0 39.0 37.0 39.0 12-13 38.43227337010599 39.0 39.0 39.0 37.0 39.0 14-15 40.02843604961849 41.0 40.0 41.0 38.0 41.0 16-17 40.02350628900301 41.0 40.0 41.0 38.0 41.0 18-19 40.10813966878737 41.0 40.0 41.0 38.0 41.0 20-21 40.10710843014944 41.0 40.0 41.0 38.0 41.0 22-23 40.05556784760958 41.0 40.0 41.0 38.0 41.0 24-25 40.03578022842518 41.0 40.0 41.0 38.0 41.0 26-27 39.94831385366245 41.0 40.0 41.0 38.0 41.0 28-29 39.877277426483815 41.0 40.0 41.0 38.0 41.0 30-31 39.751275462342974 41.0 40.0 41.0 37.0 41.0 32-33 39.58084678019349 41.0 40.0 41.0 36.0 41.0 34-35 39.48573798257634 41.0 40.0 41.0 35.5 41.0 36-37 39.399387596929316 41.0 40.0 41.0 35.0 41.0 38-39 39.29827121964348 41.0 40.0 41.0 35.0 41.0 40-41 39.21686935616809 41.0 40.0 41.0 35.0 41.0 42-43 39.09038800192015 41.0 40.0 41.0 35.0 41.0 44-45 38.89770164467683 41.0 40.0 41.0 35.0 41.0 46-47 38.62594956117367 41.0 40.0 41.0 35.0 41.0 48-49 38.37376060513135 41.0 39.0 41.0 35.0 41.0 50-51 38.42884065228108 41.0 40.0 41.0 35.0 41.0 52-53 38.41333002094825 41.0 40.0 41.0 35.0 41.0 54-55 38.329581669053916 41.0 39.5 41.0 35.0 41.0 56-57 38.237082021641775 41.0 39.0 41.0 35.0 41.0 58-59 38.10821018636048 41.0 39.0 41.0 35.0 41.0 60-61 37.975669496426896 41.0 39.0 41.0 35.0 41.0 62-63 37.75775855041748 41.0 38.5 41.0 34.5 41.0 64-65 37.50723031556937 40.0 37.5 41.0 34.0 41.0 66-67 37.218036454350546 39.5 37.0 41.0 34.0 41.0 68-69 36.84322067342342 39.0 37.0 41.0 34.0 41.0 70-71 36.46108400389723 39.0 36.0 41.0 33.5 41.0 72-73 36.048277365670494 37.5 36.0 40.5 33.0 41.0 74-75 35.60028310541277 37.0 35.0 39.0 32.5 41.0 76-77 35.1966243431838 37.0 35.0 39.0 32.0 41.0 78-79 34.79011058966924 36.0 35.0 38.5 32.0 40.5 80-81 34.39622659819345 36.0 35.0 37.0 32.0 39.0 82-83 34.033169137369526 35.5 35.0 37.0 31.5 39.0 84-85 33.68033607769613 35.0 35.0 36.5 31.0 38.0 86-87 33.45556098993734 35.0 35.0 36.0 31.0 37.0 88-89 33.28114385973085 35.0 35.0 36.0 31.0 37.0 90-91 33.2180176927944 35.0 35.0 36.0 32.0 36.5 92-93 33.10374105429594 35.0 35.0 35.5 32.0 36.0 94-95 32.99121312222054 35.0 35.0 35.0 31.0 36.0 96-97 32.911671887554874 35.0 35.0 35.0 31.0 36.0 98-99 32.85900755249678 35.0 35.0 35.0 31.0 36.0 100-101 32.824435180117405 35.0 35.0 35.0 31.0 36.0 102-103 32.38341245534426 35.0 34.0 35.0 30.0 35.0 104-105 32.73706002533457 35.0 35.0 35.0 31.0 36.0 106-107 32.763326203942 35.0 35.0 35.0 31.0 36.0 108-109 32.747204204658814 35.0 35.0 35.0 31.0 36.0 110-111 32.689350681950216 35.0 35.0 35.0 31.0 36.0 112-113 32.648444020045105 35.0 35.0 35.0 31.0 36.0 114-115 32.58413264032026 35.0 35.0 35.0 31.0 36.0 116-117 32.50519759800325 35.0 35.0 35.0 31.0 36.0 118-119 32.428132241798934 35.0 35.0 35.0 31.0 36.0 120-121 32.31663554836147 35.0 35.0 35.0 30.0 35.0 122-123 32.21075050106294 35.0 35.0 35.0 30.0 35.0 124-125 31.209210759558367 34.5 33.0 35.0 24.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1111846148739275 1101 2 0.116706943145239 1101 3 0.1105607169077274 1101 4 -0.15059048447928802 1101 5 -0.0014177291935482117 1101 6 0.08162053219196963 1101 7 0.10628003523423502 1101 8 0.13629928928091317 1101 9 0.1355008790206469 1101 10-11 0.22164957443223443 1101 12-13 0.2216679292501169 1101 14-15 0.18254242614298022 1101 16-17 0.17952608186419639 1101 18-19 0.197054868003967 1101 20-21 0.22930235539815413 1101 22-23 0.2398505923388896 1101 24-25 0.13544257428913653 1101 26-27 0.1495299646597772 1101 28-29 0.20529087421044068 1101 30-31 0.1550500124723868 1101 32-33 0.10715403637159682 1101 34-35 0.04620428516030728 1101 36-37 0.15806702328919897 1101 38-39 0.16011666542215153 1101 40-41 0.1146170561809754 1101 42-43 -0.03770807526372266 1101 44-45 -0.0719776513184982 1101 46-47 -0.017639714835041787 1101 48-49 0.04150672215373419 1101 50-51 -0.012310425262334945 1101 52-53 -0.04501107281807748 1101 54-55 -0.05848949044957408 1101 56-57 0.011682609676732625 1101 58-59 -0.00807617794299631 1101 60-61 -0.04127441549919553 1101 62-63 -0.0988337654578828 1101 64-65 -0.0647423893745227 1101 66-67 -0.07062162028378083 1101 68-69 -5.243432368402523E-4 1101 70-71 -0.030038764835289555 1101 72-73 0.018566737148688617 1101 74-75 -0.09909180877843937 1101 76-77 -0.0315301417034064 1101 78-79 -0.10956634472280768 1101 80-81 -0.11671635468235309 1101 82-83 -0.09908992966708752 1101 84-85 -0.15341717445370762 1101 86-87 -0.17449780760214395 1101 88-89 -0.15017968825850403 1101 90-91 -0.1493749107047151 1101 92-93 -0.2056416608996443 1101 94-95 -0.18646874731208385 1101 96-97 -0.1490831593425881 1101 98-99 -0.2456286668956409 1101 100-101 -0.1876145397819471 1101 102-103 -0.123918818690548 1101 104-105 -0.06655736180390193 1101 106-107 -0.0865890583838933 1101 108-109 -0.10470122436594664 1101 110-111 -0.22010898654862388 1101 112-113 -0.22704323206036037 1101 114-115 -0.23263688985974795 1101 116-117 -0.28640374942260394 1101 118-119 -0.19409265683928822 1101 120-121 -0.21875805940808135 1101 122-123 -0.11233916958780554 1101 124-125 -0.11094630034078534 1102 1 0.2887049619991586 1102 2 0.2467457758621947 1102 3 0.2123561377609633 1102 4 0.17915140543977515 1102 5 0.2414864435748072 1102 6 0.18489841063044565 1102 7 0.19793291903167187 1102 8 0.18816838173256656 1102 9 0.22402187373388927 1102 10-11 0.24643222629388362 1102 12-13 0.2831876626333951 1102 14-15 0.3658498860410333 1102 16-17 0.3620505694912026 1102 18-19 0.3562853664662313 1102 20-21 0.3948237088796702 1102 22-23 0.320253608700078 1102 24-25 0.3015049006870356 1102 26-27 0.35510305749050985 1102 28-29 0.4157581078362753 1102 30-31 0.2735913373878276 1102 32-33 0.3704420009370253 1102 34-35 0.4292012638350329 1102 36-37 0.39157045189543993 1102 38-39 0.44256996349540856 1102 40-41 0.39587220360315456 1102 42-43 0.4439766915256129 1102 44-45 0.5146316049029664 1102 46-47 0.5249781202353461 1102 48-49 0.41889597219265795 1102 50-51 0.40079601158203815 1102 52-53 0.35888020911286844 1102 54-55 0.46156881503596026 1102 56-57 0.5024900188056947 1102 58-59 0.519262415820755 1102 60-61 0.55244702688465 1102 62-63 0.604092565200105 1102 64-65 0.5626649118983167 1102 66-67 0.583652686266781 1102 68-69 0.5885584961320021 1102 70-71 0.6212977033956761 1102 72-73 0.5374985328177431 1102 74-75 0.5311658951497407 1102 76-77 0.46010423957926605 1102 78-79 0.5367817778360546 1102 80-81 0.5106458185057008 1102 82-83 0.5373333988450639 1102 84-85 0.5363283708402236 1102 86-87 0.601875609248232 1102 88-89 0.5368621206556412 1102 90-91 0.4865305730345071 1102 92-93 0.47499421637904504 1102 94-95 0.45620876965043067 1102 96-97 0.48106567308104076 1102 98-99 0.46538461655611485 1102 100-101 0.46772196906428576 1102 102-103 0.49989064602267064 1102 104-105 0.46073497567294197 1102 106-107 0.49489477560793205 1102 108-109 0.4953054320265551 1102 110-111 0.41756495401318716 1102 112-113 0.45253890443856193 1102 114-115 0.497018805417369 1102 116-117 0.47652036995923197 1102 118-119 0.5186010836697079 1102 120-121 0.5558327980932276 1102 122-123 0.4850370835626947 1102 124-125 0.562214673794049 1103 1 0.18670329449287237 1103 2 0.18732381537637366 1103 3 0.1871433547193746 1103 4 -0.03484926772239305 1103 5 0.05706420810444257 1103 6 0.09729603388402097 1103 7 0.049876839783323135 1103 8 0.12873982161764275 1103 9 0.1789354075932863 1103 10-11 0.20393556460626883 1103 12-13 0.1624941956062358 1103 14-15 0.22046940642398738 1103 16-17 0.20744380839117582 1103 18-19 0.23533836368778793 1103 20-21 0.17379883047784261 1103 22-23 0.2591448930098963 1103 24-25 0.30229960602459727 1103 26-27 0.2794278604365843 1103 28-29 0.3129875835397442 1103 30-31 0.29919734833001144 1103 32-33 0.2819728729020241 1103 34-35 0.36284584300968703 1103 36-37 0.33891421117616716 1103 38-39 0.4060217675040221 1103 40-41 0.3286648806546921 1103 42-43 0.4088369663781677 1103 44-45 0.24118257273857324 1103 46-47 0.1944802442159812 1103 48-49 0.3155441305661597 1103 50-51 0.29391171847931474 1103 52-53 0.20453853311505554 1103 54-55 0.14125636659557017 1103 56-57 0.2859435387178806 1103 58-59 0.22596444916648295 1103 60-61 0.14683272240856127 1103 62-63 -0.02333967310809726 1103 64-65 -0.01760747679343666 1103 66-67 0.052917113605964516 1103 68-69 0.07803868734525565 1103 70-71 0.054501207358747195 1103 72-73 -0.023660780028876616 1103 74-75 0.026047332812936475 1103 76-77 0.07316468558219924 1103 78-79 0.044415898798447984 1103 80-81 0.12345767847916278 1103 82-83 0.06713677389538475 1103 84-85 0.009954053076882019 1103 86-87 0.022209286495574077 1103 88-89 0.09982478215405877 1103 90-91 0.05580138001516133 1103 92-93 0.11061879427776944 1103 94-95 0.09050572216170849 1103 96-97 0.03026755793511171 1103 98-99 0.002703869214116139 1103 100-101 0.03614637361275186 1103 102-103 0.14882887569417136 1103 104-105 0.11928886494436597 1103 106-107 0.1805560914419857 1103 108-109 0.17541370994133132 1103 110-111 0.2822335096403563 1103 112-113 0.33973914378510983 1103 114-115 0.24242491359408547 1103 116-117 0.19107431202768765 1103 118-119 0.1714127456543082 1103 120-121 0.24025943331631794 1103 122-123 0.28009935106326367 1103 124-125 0.2202933897702124 1104 1 0.1987782297449172 1104 2 0.21824428977619448 1104 3 0.17895223279742822 1104 4 -0.03224338858666442 1104 5 0.020549978450837614 1104 6 0.1078185078624756 1104 7 0.11300742742024283 1104 8 0.09984105826637091 1104 9 0.13124005178278253 1104 10-11 0.13931050688109536 1104 12-13 0.13430569126806802 1104 14-15 0.28998806894508533 1104 16-17 0.276579409871502 1104 18-19 0.16410603503843646 1104 20-21 0.16611845661025626 1104 22-23 0.18152342541792876 1104 24-25 0.17559737852464252 1104 26-27 0.17757458984013397 1104 28-29 0.11402142272645932 1104 30-31 0.19034044503802505 1104 32-33 0.2729666822099759 1104 34-35 0.3211831665621858 1104 36-37 0.35898128942064034 1104 38-39 0.24895556817183007 1104 40-41 0.21399342998125093 1104 42-43 0.23667763001013498 1104 44-45 0.28364808128402785 1104 46-47 0.2265589462349169 1104 48-49 0.3427430605489832 1104 50-51 0.33599310192554555 1104 52-53 0.34240060560297536 1104 54-55 0.3299006284475823 1104 56-57 0.3636352341673259 1104 58-59 0.47108680524725344 1104 60-61 0.40407097999157315 1104 62-63 0.34646851971631776 1104 64-65 0.31271244301911594 1104 66-67 0.33699624550452256 1104 68-69 0.3240330393230195 1104 70-71 0.27315936581407385 1104 72-73 0.2934845001270716 1104 74-75 0.2880562964412974 1104 76-77 0.15582074571427285 1104 78-79 0.18986045177685895 1104 80-81 0.2777610500547567 1104 82-83 0.361113245435007 1104 84-85 0.35125292794147356 1104 86-87 0.2839176536127681 1104 88-89 0.27252678832985566 1104 90-91 0.3146612626707892 1104 92-93 0.2779398568890912 1104 94-95 0.21212760260834784 1104 96-97 0.2535011677108301 1104 98-99 0.3083519978545297 1104 100-101 0.2908750493494523 1104 102-103 0.3000209403719012 1104 104-105 0.28898597006695326 1104 106-107 0.24476829448987303 1104 108-109 0.2769227576303095 1104 110-111 0.26355875187326205 1104 112-113 0.32789404515752807 1104 114-115 0.38468101543725197 1104 116-117 0.28503386503801664 1104 118-119 0.3350122638486397 1104 120-121 0.35470955983287666 1104 122-123 0.30052342091656925 1104 124-125 0.4071643982209636 1105 1 0.11152567310422512 1105 2 0.11896483497365296 1105 3 0.09053541815798383 1105 4 0.08466846074416878 1105 5 0.06114487010188441 1105 6 0.10719989946419872 1105 7 0.11358904262526437 1105 8 0.12050483946396184 1105 9 0.10346946712591887 1105 10-11 0.10965943490056418 1105 12-13 0.10360134086854345 1105 14-15 0.24938444279006688 1105 16-17 0.24888178401243266 1105 18-19 0.22990870512492023 1105 20-21 0.20212670609235772 1105 22-23 0.19986691132763212 1105 24-25 0.17703548891233112 1105 26-27 0.1440967775353812 1105 28-29 0.15808237106752188 1105 30-31 0.22395133007962187 1105 32-33 0.23402877734002914 1105 34-35 0.24025130802416328 1105 36-37 0.049262972035485575 1105 38-39 0.09730043105039243 1105 40-41 0.05301395298280198 1105 42-43 0.11870274016764881 1105 44-45 0.08122441057276575 1105 46-47 0.16487176889777544 1105 48-49 0.2209260165517506 1105 50-51 0.17829471565084276 1105 52-53 0.20952413820631222 1105 54-55 0.24064016348424389 1105 56-57 0.19128754336465903 1105 58-59 0.2107256283704686 1105 60-61 0.18776561351234022 1105 62-63 0.15842235632149482 1105 64-65 0.13090631201389158 1105 66-67 0.2398331096326345 1105 68-69 0.22163947696071773 1105 70-71 0.19817775965183415 1105 72-73 0.18345543991859614 1105 74-75 0.1882162920021102 1105 76-77 0.10318354207085179 1105 78-79 0.15197890809200487 1105 80-81 0.06784471632683164 1105 82-83 0.06300770796501354 1105 84-85 0.10756900189498708 1105 86-87 0.20427541889505818 1105 88-89 0.18635628259180237 1105 90-91 0.12391202719678773 1105 92-93 0.08324005665233614 1105 94-95 0.1031317828391849 1105 96-97 0.17162598274485674 1105 98-99 0.16345017367906678 1105 100-101 0.17428278010476816 1105 102-103 0.16547772410147132 1105 104-105 0.22781258708053542 1105 106-107 0.1505435485098019 1105 108-109 0.19668092490530142 1105 110-111 0.1786711906289753 1105 112-113 0.21960058226581936 1105 114-115 0.31526020553294387 1105 116-117 0.32097994540367836 1105 118-119 0.3662627776429943 1105 120-121 0.30345497678726474 1105 122-123 0.2846893066158245 1105 124-125 0.23334982454696274 1106 1 0.1953266854918283 1106 2 0.13408383767755083 1106 3 0.17089564893205278 1106 4 0.09684973157357746 1106 5 0.09224552721060775 1106 6 0.14373781683827502 1106 7 0.12726963748158227 1106 8 0.18299108419765986 1106 9 0.1480149145257812 1106 10-11 0.18732363347944414 1106 12-13 0.15338754472945482 1106 14-15 0.18427781982585145 1106 16-17 0.26808020569387736 1106 18-19 0.2535913436208048 1106 20-21 0.29046793887862066 1106 22-23 0.2658627140063743 1106 24-25 0.30617484155019525 1106 26-27 0.2648110295632975 1106 28-29 0.2517478744316648 1106 30-31 0.35983272824349655 1106 32-33 0.248716454193449 1106 34-35 0.3212010502111724 1106 36-37 0.406001873500621 1106 38-39 0.5029972728412488 1106 40-41 0.5446576287043641 1106 42-43 0.6338600611133813 1106 44-45 0.6550646451197153 1106 46-47 0.6849839332692795 1106 48-49 0.7022669978131475 1106 50-51 0.6692683133996908 1106 52-53 0.6912748298996974 1106 54-55 0.7749575878695794 1106 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-0.3593115497797612 2216 76-77 -0.3795449525528056 2216 78-79 -0.3410846479755776 2216 80-81 -0.38601953098499564 2216 82-83 -0.3561359172269931 2216 84-85 -0.3292061517148426 2216 86-87 -0.31546344439218643 2216 88-89 -0.5383941014327718 2216 90-91 -0.45465940137464145 2216 92-93 -0.403602301258573 2216 94-95 -0.5299759360548251 2216 96-97 -0.4221432184840168 2216 98-99 -0.3595096957525783 2216 100-101 -0.4126699848721884 2216 102-103 -0.5236447797387811 2216 104-105 -0.4758740488641635 2216 106-107 -0.4218546849771485 2216 108-109 -0.3472562870762559 2216 110-111 -0.351901966053056 2216 112-113 -0.25506339622881313 2216 114-115 -0.2768960924701318 2216 116-117 -0.38599970129371286 2216 118-119 -0.33379962635086 2216 120-121 -0.2671431414923049 2216 122-123 -0.29541822026589415 2216 124-125 -0.474378670076689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 0.0 4 11.0 5 34.0 6 100.0 7 239.0 8 380.0 9 716.0 10 1484.0 11 2087.0 12 2568.0 13 4141.0 14 7771.0 15 7361.0 16 2185.0 17 1329.0 18 1044.0 19 1044.0 20 1334.0 21 1748.0 22 1765.0 23 1200.0 24 1366.0 25 1702.0 26 2123.0 27 2657.0 28 3345.0 29 4255.0 30 5319.0 31 6878.0 32 8687.0 33 11378.0 34 16196.0 35 24958.0 36 49825.0 37 288323.0 38 306929.0 39 368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.59126826983055 25.122877309045165 15.286092540396156 21.999761880728126 2 13.621477399327924 23.40395248120223 42.824369105276574 20.15020101419327 3 14.844919564680142 31.854146368603764 32.151484686041535 21.14944938067456 4 10.434497530837001 20.23547431124541 44.02573993901925 25.30428821889833 5 9.947679897275522 40.46653170272033 38.38783667636612 11.19795172363802 6 24.522940030074253 44.50976636808092 19.212453122209656 11.754840479635174 7 23.395660516757953 34.461226332943866 27.60588310644789 14.537230043850286 8 22.241113168412785 37.878029182630165 23.521039467844634 16.359818181112416 9 24.9765480548148 17.34809932497215 25.262500048521265 32.412852571691786 10-11 20.536464054799268 29.53534741873335 32.12968246389694 17.79850606257044 12-13 22.073617758524538 28.22669652988845 31.172454930213476 18.527230781373525 14-15 20.84247150507791 29.352972153968977 28.660476647038198 21.144079693914914 16-17 18.017175234228326 32.19088395395267 30.582048166737184 19.20989264508182 18-19 17.836999600184768 30.69532914885936 33.360246462152766 18.107424788803105 20-21 17.90557632265736 30.41934018841778 33.78923914773367 17.88584434119119 22-23 18.867591287909665 29.572029495754066 33.15264013912017 18.407739077216096 24-25 18.660502525046677 30.448970508127637 33.11362904133623 17.77689792548945 26-27 17.997249167698552 30.468249624445402 33.71606907875584 17.81843212910021 28-29 17.995955267274542 28.576308424456272 34.81750181469535 18.610234493573845 30-31 18.80056724594589 29.370569199735524 33.70190086911292 18.126962685205672 32-33 18.36375834323707 29.82791760177989 33.79754197577429 18.010782079208752 34-35 18.773791686398724 29.308705137063406 33.869980617296406 18.04752255924146 36-37 19.330089493491297 28.696790254765 32.89586135463632 19.077258897107395 38-39 18.42026605800173 30.233722677038816 32.9695447564111 18.376466508548354 40-41 18.073977462842414 29.11055990953048 34.72699348003576 18.08846914759134 42-43 18.662996257347018 28.912883848365944 33.205774748575884 19.21834514571115 44-45 18.196727568915332 29.436805695280004 33.097410171824954 19.26905656397971 46-47 19.141186532566827 29.202038669508074 32.50769547277181 19.149079325153295 48-49 18.694535180796628 29.88929841552656 31.76341652611428 19.652749877562535 50-51 18.640715645725088 29.33082570077526 31.857414304762756 20.1710443487369 52-53 18.584744655220824 29.791798482772364 32.4492111736065 19.174245688400312 54-55 19.246768807166138 29.797815119744016 31.43544019139375 19.519975881696098 56-57 18.717405438977288 30.128647631000945 31.378376717269578 19.775570212752193 58-59 18.181424124971777 29.256974056297082 32.605633848377394 19.95596797035375 60-61 19.27912412669187 30.70673991057791 30.852628221592376 19.16150774113785 62-63 18.696238367820136 29.986957466850228 32.3093176477895 19.007486517540137 64-65 18.754747058602803 29.399286016507798 31.9360753163304 19.909891608559 66-67 18.537519821078178 30.113741126206005 30.988747514884814 20.359991537831007 68-69 18.48947500195704 29.61348928415004 31.82135484158967 20.07568087230325 70-71 19.115571088272095 29.243616799659446 32.04403447503976 19.59677763702871 72-73 19.08341387863473 29.32560616000114 31.239673057240864 20.351306904123273 74-75 18.71117083184265 29.931041532289633 30.5361991521065 20.821588483761218 76-77 18.46791067082284 29.83694927842842 31.95974001943514 19.735400031313606 78-79 18.595511033505186 29.077227743269884 31.67281382363083 20.6544473995941 80-81 18.58345075479681 30.286909480020235 30.521428931872265 20.60821083331069 82-83 19.34445828917898 29.5293954767829 30.900477061086335 20.22566917295179 84-85 18.628955458714174 29.08556057014502 30.73075708799569 21.554726883145115 86-87 18.243113853033424 29.47146687093068 31.679381411284897 20.606037864751002 88-89 19.053848253946075 28.705569076814985 31.68872119939394 20.551861469845004 90-91 18.743841838685448 28.716327028368138 31.837928515837028 20.701902617109386 92-93 19.208257720587046 29.331922973659267 31.189521933357273 20.270297372396413 94-95 18.713269069030215 28.005110952966294 31.725625929999353 21.55599404800414 96-97 19.2173300407774 28.02125108930594 31.069172787374594 21.69224608254206 98-99 18.564883283712252 28.229931280948477 31.282954026424036 21.922231408915234 100-101 18.45655494461772 28.322888301888256 31.52782117743873 21.69273557605529 102-103 19.346916699984604 27.839173352897106 30.905458577718775 21.908451369399515 104-105 18.516426712833034 28.289321310410593 31.222593571643912 21.97165840511246 106-107 18.922295306173016 27.82544731841852 30.915934479343164 22.336322896065305 108-109 19.000212847308255 28.19029972653326 30.045888326974868 22.763599099183608 110-111 18.483044081893546 28.09187210570649 30.262260676942827 23.162823135457142 112-113 17.88222142000396 27.914090187447353 31.835126032370802 22.368562360177886 114-115 18.144953114668827 28.439023190997396 30.615597217028558 22.800426477305223 116-117 17.950803127110685 28.658129478530224 30.352498660807864 23.038568733551227 118-119 18.206984626472384 27.438929319813752 30.64735988468722 23.70672616902664 120-121 17.746297180461585 27.91564286795617 30.553388272345337 23.78467167923691 122-123 17.82861231587773 28.397722214746345 30.616117362095913 23.15754810728001 124-125 17.77778927918929 28.001286145342185 30.601072391609257 23.619852183859262 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 220.5 2 245.5 3 362.5 4 225.0 5 146.5 6 310.0 7 748.5 8 1276.0 9 1340.5 10 864.5 11 447.0 12 357.5 13 376.0 14 411.5 15 439.0 16 467.0 17 491.5 18 561.0 19 650.5 20 728.5 21 873.5 22 1007.0 23 1183.0 24 1346.0 25 1729.5 26 2991.0 27 4562.5 28 5136.0 29 6443.0 30 10002.0 31 13352.0 32 19635.5 33 29024.5 34 36808.5 35 43503.0 36 54434.5 37 63218.0 38 62075.5 39 58704.5 40 52147.5 41 44178.0 42 38468.0 43 35329.0 44 34001.0 45 33004.0 46 28083.5 47 20121.0 48 15708.0 49 12608.0 50 9904.0 51 6587.0 52 3993.0 53 2723.0 54 1857.0 55 1544.5 56 1263.0 57 978.5 58 773.5 59 634.0 60 543.0 61 468.5 62 337.5 63 236.0 64 150.5 65 83.0 66 57.0 67 38.0 68 27.5 69 23.0 70 15.5 71 18.0 72 19.0 73 15.0 74 14.5 75 13.0 76 6.5 77 5.5 78 6.5 79 3.0 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01746765572415078 2 0.0033641411024290394 3 0.0 4 0.14724586825247102 5 0.039463962932340654 6 0.01332717436731504 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 6.469502120055846E-5 34-35 3.881701272033507E-4 36-37 9.704253180083768E-4 38-39 0.002329020763220104 40-41 0.0 42-43 0.0012292054028106105 44-45 8.410352756072598E-4 46-47 0.0 48-49 0.001746765572415078 50-51 0.002134935699618429 52-53 0.0 54-55 0.0 56-57 0.0012292054028106105 58-59 0.0013585954452117273 60-61 7.116452332061429E-4 62-63 1.293900424011169E-4 64-65 0.001293900424011169 66-67 7.116452332061429E-4 68-69 8.410352756072598E-4 70-71 0.0010351203392089353 72-73 0.0 74-75 6.469502120055846E-5 76-77 0.0038817012720335073 78-79 8.410352756072598E-4 80-81 0.0 82-83 0.0 84-85 1.293900424011169E-4 86-87 1.293900424011169E-4 88-89 0.0 90-91 6.469502120055846E-5 92-93 5.82255190805026E-4 94-95 9.057302968078183E-4 96-97 3.2347510600279225E-4 98-99 0.0 100-101 1.9408506360167534E-4 102-103 0.0 104-105 0.0 106-107 1.9408506360167534E-4 108-109 3.2347510600279225E-4 110-111 0.0 112-113 0.0 114-115 0.0018114605936156367 116-117 3.881701272033507E-4 118-119 3.2347510600279225E-4 120-121 0.0 122-123 1.293900424011169E-4 124-125 6.469502120055845E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 772857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.057776619666248 #Duplication Level Percentage of deduplicated Percentage of total 1 66.85307639379268 8.729525378876277 2 11.632036229200365 3.037770614255266 3 4.706796026860244 1.8438087333922109 4 2.6847417330314727 1.4022703132568244 5 1.7834099275685478 1.1643684227742632 6 1.2958390087409086 1.0152465786713116 7 0.9501483701831521 0.868477762137409 8 0.7013713295047395 0.7326680118488976 9 0.6054572998558373 0.7115333556837429 >10 5.869738672820139 16.876412700261465 >50 1.3597811631813224 12.656265838559703 >100 1.430717579482479 37.482439549178565 >500 0.10390686331436998 9.081508692615134 >1k 0.0229794024637549 4.397704048488939 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4089 0.529075883378167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2776 0.35918675770550046 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.2564510640390137 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1717 0.22216270280271772 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1715 0.22190392271791548 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1402 0.1814048394463659 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1313 0.16988912567266648 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1306 0.16898339537585866 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1303 0.1685952252486553 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1299 0.16807766507905084 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1257 0.16264328329820393 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1249 0.161608162958995 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1231 0.1592791421957749 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1179 0.15255085999091683 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1171 0.15151573965170786 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1166 0.15086878943970228 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1164 0.15061000935490007 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1156 0.14957488901569113 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1111 0.14375233710764088 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1051 0.13598893456357386 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1030 0.1332717436731504 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1025 0.1326247934611448 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1017 0.1315896731219359 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 980 0.12680224155309455 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 976 0.12628468138349008 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 975 0.12615529134108897 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 942 0.1218854199418521 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 935 0.1209796896450443 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 917 0.1186506688818242 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 913 0.11813310871221974 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 886 0.11463957756738957 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 872 0.11282811697377393 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 861 0.11140482650736165 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 860 0.11127543646496053 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 859 0.1111460464225594 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 852 0.11024031612575158 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 844 0.10920519578654267 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 840 0.10868763561693819 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 827 0.10700556506572367 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 823 0.10648800489611922 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 819 0.10597044472651473 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 817 0.1057116646417125 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 814 0.10532349451450915 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 783 0.10131240320007454 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 782 0.10118301315767342 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 776 0.10040667290326671 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 775 0.1002772828608656 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.2939004240111688E-4 0.0 16-17 0.0 0.0 0.0 1.2939004240111688E-4 0.0 18-19 0.0 0.0 0.0 1.2939004240111688E-4 0.0 20-21 0.0 0.0 0.0 2.5878008480223377E-4 0.0 22-23 0.0 0.0 0.0 7.763402544067014E-4 0.0 24-25 0.0 0.0 0.0 0.002005545657217312 0.0 26-27 0.0 0.0 0.0 0.004658041526440208 0.0 28-29 0.0 0.0 0.0 0.010998153604094937 0.0 30-31 0.0 0.0 0.0 0.0317652554094742 0.0 32-33 0.0 0.0 0.0 0.0750462245926478 0.0 34-35 0.0 0.0 0.0 0.1334011337155515 0.0 36-37 0.0 0.0 0.0 0.21776344136107972 0.0 38-39 0.0 0.0 0.0 0.34792982401660333 0.0 40-41 0.0 0.0 0.0 0.4943346569934671 0.0 42-43 0.0 0.0 0.0 0.6315527969598516 0.0 44-45 0.0 0.0 0.0 0.7960722358728718 0.0 46-47 0.0 0.0 0.0 0.9860815131389119 0.0 48-49 0.0 0.0 0.0 1.1784845061893727 0.0 50-51 0.0 0.0 0.0 1.3808505325047196 0.0 52-53 0.0 0.0 0.0 1.592338556809345 0.0 54-55 0.0 0.0 0.0 1.8018210354567534 0.0 56-57 0.0 0.0 0.0 2.052126072481714 0.0 58-59 0.0 0.0 0.0 2.318798949870416 0.0 60-61 0.0 0.0 0.0 2.5985402215416302 0.0 62-63 0.0 0.0 0.0 2.872458941304795 0.0 64-65 0.0 0.0 0.0 3.1807954123466566 0.0 66-67 0.0 0.0 0.0 3.5211559188827946 0.0 68-69 0.0 0.0 0.0 3.8912114401499887 0.0 70-71 0.0 0.0 0.0 4.312052553059622 0.0 72-73 0.0 0.0 0.0 4.711738394036672 0.0 74-75 0.0 0.0 0.0 5.14254254021119 0.0 76-77 0.0 0.0 0.0 5.612551869233247 0.0 78-79 0.0 0.0 0.0 6.144927198692644 0.0 80-81 0.0 0.0 0.0 6.724465198607246 0.0 82-83 0.0 0.0 0.0 7.295204675638573 0.0 84-85 0.0 0.0 0.0 7.893568926722537 0.0 86-87 0.0 0.0 0.0 8.533596771459662 0.0 88-89 0.0 0.0 0.0 9.219428691206783 0.0 90-91 0.0 0.0 0.0 9.959798513825973 0.0 92-93 0.0 0.0 0.0 10.77023304440537 0.0 94-95 0.0 0.0 0.0 11.5965178551789 0.0 96-97 0.0 0.0 0.0 12.484133546050563 0.0 98-99 0.0 0.0 0.0 13.382359220399117 0.0 100-101 0.0 0.0 0.0 14.351037772835078 0.0 102-103 0.0 0.0 0.0 15.376065688736727 0.0 104-105 0.0 0.0 0.0 16.412674013433275 0.0 106-107 0.0 0.0 0.0 17.494633547991413 0.0 108-109 0.0 0.0 0.0 18.54605703254289 0.0 110-111 0.0 0.0 0.0 19.700345600803253 0.0 112-113 0.0 0.0 0.0 20.91965266537018 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1620 0.0 68.477745 1 GTATGGT 140 0.0 59.641903 1 GTATCAA 3385 0.0 51.62505 1 GGATGCC 150 0.0 51.562572 8 GGGATGC 165 0.0 46.87507 7 GGGCCTA 95 1.4876059E-7 43.838627 7 TCAACGC 4005 0.0 43.020733 4 CAACGCA 4025 0.0 42.88836 5 ATCAACG 4060 0.0 42.437946 3 AACGCAG 4135 0.0 41.87242 6 GGTACTC 200 0.0 41.646694 8 GTGGTAC 210 0.0 39.666084 6 TGGTACT 210 0.0 39.663517 7 ACATATA 340 0.0 38.584312 2 GACATAT 320 0.0 37.27619 1 GTCTTTA 80 1.1804392E-4 37.27619 1 ACGCAGA 4655 0.0 36.80911 7 CGCAGAG 4665 0.0 36.7302 8 GTATTGA 65 0.0019159667 36.70271 1 GGATGAC 360 0.0 36.358227 8 >>END_MODULE