FastQCFastQC Report
Fri 27 May 2016
SRR939997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375419
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.046.7747651
TCAACGC20550.042.632054
ATCAACG20700.042.3184973
CAACGCA20900.041.329735
AACGCAG22850.037.8026856
TATCAAC24800.035.8328972
GGTATCA14350.035.7458421
GTACATG53650.034.0197141
TACATGG53600.033.270072
ACATGGG55650.030.946813
ACGCAGA29400.029.1780347
CGCAGAG31000.027.2877358
CATGGGG38200.026.3665944
GTATAGG5450.024.0771521
AGTACAT38150.023.7627322
GAGTACA40900.022.7499391
GTACGGG1350.00251950322.090991
ATGGGGA22300.021.9053345
GTCTTAG6550.021.8549041
CATGGGA25250.021.4788344
Produced by FastQC (version 0.11.5)