FastQCFastQC Report
Fri 27 May 2016
SRR939997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375419
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.19455889441690133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.14562835034269558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35750.061.189271
GGTATCA20600.059.3160061
TCAACGC43650.049.0171474
CAACGCA44150.048.66825
ATCAACG44100.048.516973
TATCAAC46650.045.9926722
AACGCAG46850.045.8484086
ACGCAGA55400.038.4447257
CGCAGAG57000.037.3655748
GTACATG53200.032.37961
TACATGG52700.032.1177832
GTGGTAT9600.031.0444531
GCAGAGT67900.031.01689
ACATGGG53950.029.6060963
GAGTACT40250.026.30980712-13
TAACGCA1401.044223E-425.542364
CAGAGTA63250.025.34905210-11
AGAGTAC62300.024.97164510-11
CATGGGG37350.024.733124
GTACTTT42500.024.2870114-15
Produced by FastQC (version 0.11.5)