FastQCFastQC Report
Fri 27 May 2016
SRR939996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951878
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.044.9437071
GGTATCA18250.044.136971
ATCAACG29100.038.9255833
CAACGCA29500.038.3790475
TCAACGC30250.037.450584
TATCAAC33700.034.5243232
AACGCAG32650.034.4938166
GTACATG71100.033.7355731
TACATGG71800.032.4086272
ACATGGG76900.029.304883
ACGCAGA38300.029.2497187
CGCAGAG39300.028.20228
CATGGGG49650.024.8588714
GAGTACA55650.021.0146661
AGTACAT52700.020.831842
CATGGGA33600.020.5849114
GCAGAGT53100.020.413989
GTATAAG12800.018.6458261
ATGGGGA27950.018.5481265
GTCCTAC5151.8189894E-1218.537211
Produced by FastQC (version 0.11.5)