FastQCFastQC Report
Fri 27 May 2016
SRR939996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951878
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.17034876155169534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.1224974101864973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.058.0919231
GTATCAA44600.057.220411
ATCAACG54250.046.9212763
TCAACGC54650.046.467574
CAACGCA55550.045.540785
AACGCAG58000.043.701786
TATCAAC58550.043.4753042
ACGCAGA67200.037.6254357
CGCAGAG68700.036.544138
GTACATG66450.030.598591
GCAGAGT82600.030.1783779
TACATGG66800.029.2711012
ACATGGG68400.027.976323
TATACCG1306.3626656E-527.4728375
AGAGTAC75800.025.82192210-11
GTGTACG3059.094947E-1225.414731
TGTACGC1901.1497323E-625.1002522
CAGAGTA78050.024.50586110-11
GAGTACT48550.024.38516612-13
GTCCTAC5550.023.6358761
Produced by FastQC (version 0.11.5)