FastQCFastQC Report
Fri 27 May 2016
SRR939995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449914
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44010.9781869423934352No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.17803402428019577No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.1378041136750579No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6140.13647052547820251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.049.9623151
GGTATCA10700.044.060741
ACGCAAA1655.456968E-1239.7848051
ATCAACG23150.038.8734863
TCAACGC23200.038.5328184
GAACAAA6950.037.7812541
TACAAGG2850.037.682462
GTACATG49100.037.4349941
TATCAAC26100.036.804172
CGATAGC650.001922141136.6753434
AACGCAG24400.036.621446
CAACGCA24450.036.546555
TACATGG49700.035.8943752
GTATAGA1051.4518111E-534.101261
GTACAAG4350.032.9253541
TCGACCG552.0043233E-532.53668618-19
ACGAATC552.0436308E-532.4444372-73
ACATGGG53050.032.0174673
GAGAACA8450.031.7807641
ATGGGAT14000.031.0619565
Produced by FastQC (version 0.11.5)