##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939995_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 449914 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.858337371142042 33.0 31.0 34.0 20.0 34.0 2 29.912950030450265 34.0 31.0 34.0 18.0 34.0 3 29.974397329267372 34.0 31.0 34.0 18.0 34.0 4 32.56386331610041 37.0 35.0 37.0 16.0 37.0 5 33.2349004476411 37.0 35.0 37.0 25.0 37.0 6 32.74167285303414 37.0 35.0 37.0 13.0 37.0 7 32.98153202612055 37.0 35.0 37.0 17.0 37.0 8 32.67900087572291 37.0 35.0 37.0 13.0 37.0 9 33.8742981992114 39.0 37.0 39.0 10.0 39.0 10-11 34.16498375245047 39.0 37.0 39.0 10.5 39.0 12-13 34.060332641349234 39.0 37.0 39.0 10.5 39.0 14-15 35.38511915610539 41.0 38.0 41.0 12.5 41.0 16-17 35.530234667069706 41.0 38.0 41.0 13.0 41.0 18-19 35.56359548713754 41.0 38.0 41.0 14.0 41.0 20-21 35.67257853723156 41.0 38.0 41.0 17.5 41.0 22-23 35.85557128695706 41.0 37.0 41.0 21.5 41.0 24-25 36.11182581560031 41.0 37.0 41.0 25.5 41.0 26-27 36.29374724947434 41.0 37.0 41.0 27.0 41.0 28-29 36.29063332103469 41.0 37.0 41.0 27.5 41.0 30-31 36.23136532759594 41.0 36.5 41.0 27.5 41.0 32-33 36.18397738234418 41.0 36.0 41.0 28.0 41.0 34-35 36.0668761141018 41.0 36.0 41.0 27.5 41.0 36-37 35.864994198891345 40.0 35.0 41.0 27.0 41.0 38-39 35.711696013015825 40.0 35.0 41.0 26.5 41.0 40-41 35.58033757562556 40.0 35.0 41.0 26.0 41.0 42-43 35.347189685139824 40.0 35.0 41.0 25.0 41.0 44-45 35.175303502447136 40.0 35.0 41.0 25.0 41.0 46-47 34.98764986197362 40.0 35.0 41.0 25.0 41.0 48-49 34.803563569926695 40.0 34.0 41.0 25.0 41.0 50-51 34.61382286392511 39.5 34.0 41.0 24.5 41.0 52-53 34.67625924065488 39.5 34.0 41.0 25.0 41.0 54-55 34.425934734193646 39.0 34.0 41.0 23.5 41.0 56-57 34.262849122276705 39.0 34.0 41.0 23.5 41.0 58-59 34.006382330845454 38.5 34.0 41.0 21.5 41.0 60-61 33.69586632111914 37.5 33.0 41.0 18.5 41.0 62-63 33.34035504563094 37.0 33.0 40.5 9.0 41.0 64-65 32.9790904483968 36.5 33.0 40.0 2.0 41.0 66-67 32.630592735500564 36.0 33.0 39.5 2.0 41.0 68-69 32.27219535288967 35.0 32.5 39.0 2.0 41.0 70-71 31.90488848980027 35.0 32.0 39.0 2.0 41.0 72-73 31.55875789595345 35.0 32.0 37.5 2.0 40.0 74-75 31.21204052330001 35.0 32.0 37.0 2.0 39.5 76-77 30.87645527811982 35.0 31.0 36.5 2.0 39.0 78-79 30.561195028383203 35.0 31.0 36.0 2.0 39.0 80-81 30.269761554430403 35.0 31.0 36.0 2.0 37.0 82-83 29.99182399302978 35.0 31.0 35.0 2.0 37.0 84-85 29.761932280391363 35.0 31.0 35.0 2.0 36.5 86-87 29.559879221362305 35.0 30.0 35.0 2.0 36.0 88-89 29.374409331561143 35.0 30.0 35.0 2.0 36.0 90-91 29.22564979084892 35.0 30.0 35.0 2.0 36.0 92-93 29.076770227198978 35.0 30.0 35.0 2.0 35.5 94-95 28.949062487497613 35.0 30.0 35.0 2.0 35.0 96-97 28.85133158781456 35.0 29.5 35.0 2.0 35.0 98-99 28.758902812537507 35.0 29.0 35.0 2.0 35.0 100-101 28.648630627186527 35.0 29.0 35.0 2.0 35.0 102-103 28.002360451108437 33.5 28.0 34.5 2.0 35.0 104-105 28.83106882648684 35.0 30.0 35.0 2.0 35.0 106-107 28.918515538525142 35.0 30.0 35.0 2.0 35.0 108-109 28.89065688109283 35.0 30.0 35.0 2.0 35.0 110-111 28.82639793382735 35.0 30.0 35.0 2.0 35.0 112-113 28.75524655823113 35.0 30.0 35.0 2.0 35.0 114-115 28.673707641904898 35.0 29.0 35.0 2.0 35.0 116-117 28.58147445956338 35.0 29.0 35.0 2.0 35.0 118-119 28.475137470716625 35.0 29.0 35.0 2.0 35.0 120-121 28.35076703547789 35.0 29.0 35.0 2.0 35.0 122-123 28.19615859919896 35.0 29.0 35.0 2.0 35.0 124-125 26.968068341949795 33.5 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15803693499240623 1101 2 0.4573524422721853 1101 3 0.26862784513391347 1101 4 0.19274325011814142 1101 5 0.5009002466278716 1101 6 0.2899211083731217 1101 7 0.4711092747794723 1101 8 0.4348856841562494 1101 9 0.5757744223923638 1101 10-11 0.48690128075276107 1101 12-13 0.5245945017383491 1101 14-15 0.7840641846495089 1101 16-17 0.7381203473850917 1101 18-19 0.7231638120638948 1101 20-21 0.5704011449421529 1101 22-23 0.65721004271645 1101 24-25 0.6997526729579349 1101 26-27 0.6040037115710177 1101 28-29 0.6797008906415627 1101 30-31 0.7410693269970352 1101 32-33 0.7539097608500569 1101 34-35 0.778195029949238 1101 36-37 0.8683596839431047 1101 38-39 0.8202898620911441 1101 40-41 0.8079617483642494 1101 42-43 0.7046583656888288 1101 44-45 0.6897015036362575 1101 46-47 0.5525891022410718 1101 48-49 0.6313771307334264 1101 50-51 0.6435753611983444 1101 52-53 0.5413015104357441 1101 54-55 0.6428717753052311 1101 56-57 0.6276133198880558 1101 58-59 0.574172104851705 1101 60-61 0.5125337600204176 1101 62-63 0.5407763794022529 1101 64-65 0.4255954854292199 1101 66-67 0.6445908553541528 1101 68-69 0.4901214271330403 1101 70-71 0.5592140257741924 1101 72-73 0.4921388413689627 1101 74-75 0.3538319440575286 1101 76-77 0.5925287915483644 1101 78-79 0.581449548828413 1101 80-81 0.5666906774826153 1101 82-83 0.5840451159898876 1101 84-85 0.5074683820794448 1101 86-87 0.6957119416983133 1101 88-89 0.4101865216723155 1101 90-91 0.4163190440397635 1101 92-93 0.5042472075775315 1101 94-95 0.5751896103307459 1101 96-97 0.6073768572459635 1101 98-99 0.6322924165249653 1101 100-101 0.6041768465250215 1101 102-103 0.4319898781018132 1101 104-105 0.6033800159480123 1101 106-107 0.6633494959462247 1101 108-109 0.5700673586294762 1101 110-111 0.6349109177638219 1101 112-113 0.5527721377969712 1101 114-115 0.6200331707267708 1101 116-117 0.6621862839168777 1101 118-119 0.5953519491660302 1101 120-121 0.7992882089740405 1101 122-123 0.7760477588220027 1101 124-125 0.7284760581626699 1102 1 -0.21543709098162012 1102 2 -0.24245275253300846 1102 3 -0.38257345356738526 1102 4 -0.02534116546627274 1102 5 -0.2871517014240794 1102 6 -0.192059411107401 1102 7 -0.21109851742831864 1102 8 -0.3877441859736166 1102 9 -0.09120609708815408 1102 10-11 -0.15236819976672678 1102 12-13 -0.2370288748850342 1102 14-15 -0.05052997119464919 1102 16-17 -0.10692835391360944 1102 18-19 -0.16256670741662305 1102 20-21 -0.27159560830459384 1102 22-23 -0.29973800923160354 1102 24-25 -0.13432200236673708 1102 26-27 0.044312153129460796 1102 28-29 -0.055980927540261405 1102 30-31 0.033406989334700654 1102 32-33 0.04470521539550987 1102 34-35 0.08892554942975295 1102 36-37 0.13904150212491828 1102 38-39 0.13743271923399902 1102 40-41 0.09861109901360265 1102 42-43 0.1673207033511659 1102 44-45 0.22000994519470396 1102 46-47 0.19583585548782168 1102 48-49 0.1529355722918666 1102 50-51 0.20386756899055314 1102 52-53 0.2386878740721059 1102 54-55 0.3454529441364045 1102 56-57 0.3977918913166292 1102 58-59 0.1876948321244356 1102 60-61 0.24254999378665332 1102 62-63 0.4129679378438169 1102 64-65 0.4298811997149343 1102 66-67 0.36178241379570864 1102 68-69 0.20882272583434514 1102 70-71 0.26522051928068535 1102 72-73 0.08707390630402756 1102 74-75 0.1164455804211677 1102 76-77 0.005158661678233756 1102 78-79 0.046352146231011915 1102 80-81 0.07149587228780874 1102 82-83 0.03862303806780787 1102 84-85 0.1547735768846401 1102 86-87 0.2216859676723395 1102 88-89 0.040300158035954325 1102 90-91 -0.03577511180439075 1102 92-93 -0.040119675539354915 1102 94-95 -0.05687207798094107 1102 96-97 -0.013515999896895892 1102 98-99 -0.10798355750100797 1102 100-101 -0.09241406256589002 1102 102-103 0.08953857940051435 1102 104-105 0.11562027568827205 1102 106-107 -0.038306348209619756 1102 108-109 -0.1837800439679249 1102 110-111 -0.07776765366475047 1102 112-113 -0.06152981025497439 1102 114-115 -0.005859686416087584 1102 116-117 -0.14526501478441745 1102 118-119 -0.2271642846002031 1102 120-121 -0.15548451829868526 1102 122-123 -0.3105269165026705 1102 124-125 -0.18230316261654878 1103 1 0.14974127495624145 1103 2 0.13524575149460816 1103 3 0.23605551240335387 1103 4 0.3005868305883084 1103 5 -0.06636919279346642 1103 6 0.4266977810675101 1103 7 0.4862087322839912 1103 8 0.545351326109234 1103 9 0.5370990155207522 1103 10-11 0.47845869485763615 1103 12-13 0.5449832901651135 1103 14-15 0.5496722316657809 1103 16-17 0.4727609441301155 1103 18-19 0.43626281748884566 1103 20-21 0.21060232034902526 1103 22-23 0.23040172445243456 1103 24-25 0.08968260062520272 1103 26-27 0.14698517630881014 1103 28-29 0.22267105339020077 1103 30-31 0.273980719402104 1103 32-33 0.349671514918775 1103 34-35 0.3708374350125254 1103 36-37 0.38556131142954086 1103 38-39 0.40283959084340637 1103 40-41 0.42378453317437703 1103 42-43 0.3957523982385567 1103 44-45 0.43277338428724477 1103 46-47 0.2931858472681981 1103 48-49 0.33011130794862 1103 50-51 0.20001749501390265 1103 52-53 0.18532275817534583 1103 54-55 0.2549762056849829 1103 56-57 0.15513366346851853 1103 58-59 0.2830441663345269 1103 60-61 0.250790993293478 1103 62-63 0.24049382967350397 1103 64-65 0.3470240568577978 1103 66-67 0.26948913745179937 1103 68-69 0.32909068572255507 1103 70-71 0.447753808775996 1103 72-73 0.4713431813327986 1103 74-75 0.28087760409369267 1103 76-77 0.3826960609877865 1103 78-79 0.2978826410526487 1103 80-81 0.21144655451697147 1103 82-83 0.44338055902786166 1103 84-85 0.5176175683362274 1103 86-87 0.5003231532715517 1103 88-89 0.5071914945475413 1103 90-91 0.4738122628462733 1103 92-93 0.5427078766552889 1103 94-95 0.3432840949600404 1103 96-97 0.4084731501935188 1103 98-99 0.4578688179716224 1103 100-101 0.4730963763622711 1103 102-103 0.5255816502537094 1103 104-105 0.5587371588051582 1103 106-107 0.5761207436858271 1103 108-109 0.6069909571828234 1103 110-111 0.5924493445269334 1103 112-113 0.7110790998222889 1103 114-115 0.6151168054464797 1103 116-117 0.5745393580579297 1103 118-119 0.37343739220400707 1103 120-121 0.327882241523767 1103 122-123 0.40971638450012193 1103 124-125 0.24556466575760894 1104 1 0.21021947467494684 1104 2 0.3608742676690113 1104 3 0.25379649592756337 1104 4 0.35960832948322263 1104 5 0.1708407228183475 1104 6 0.4314885686905754 1104 7 0.32587117954138023 1104 8 0.6389035412991078 1104 9 0.6117863271542703 1104 10-11 0.40783382043530025 1104 12-13 0.42429688269073296 1104 14-15 0.4817080338558597 1104 16-17 0.3914933632581068 1104 18-19 0.5263632168257928 1104 20-21 0.3357781290691406 1104 22-23 0.4756376220815284 1104 24-25 0.5018112047039622 1104 26-27 0.4399908147456202 1104 28-29 0.43039533508600414 1104 30-31 0.436456231758946 1104 32-33 0.4737014275167297 1104 34-35 0.44617618074288146 1104 36-37 0.4020402394986604 1104 38-39 0.4713563303451167 1104 40-41 0.40957385153885184 1104 42-43 0.3868756275935823 1104 44-45 0.5296816623664142 1104 46-47 0.3056148958918712 1104 48-49 0.310593400574696 1104 50-51 0.36483032151580375 1104 52-53 0.36525984376908127 1104 54-55 0.4132338784798364 1104 56-57 0.27282661353884663 1104 58-59 0.3974359937405936 1104 60-61 0.21997423621089496 1104 62-63 0.2764906651165404 1104 64-65 0.37963814415937946 1104 66-67 0.35860375217954754 1104 68-69 0.29922360967272965 1104 70-71 0.13267632736149082 1104 72-73 0.06480749216261472 1104 74-75 0.030915277390864304 1104 76-77 0.040594267738843115 1104 78-79 0.24340391390778038 1104 80-81 0.19719662986356923 1104 82-83 0.1793080128152873 1104 84-85 0.14578683446039875 1104 86-87 0.12286967979355268 1104 88-89 0.1239017994500955 1104 90-91 0.21847678213499933 1104 92-93 0.17046744567276662 1104 94-95 0.1783394119180457 1104 96-97 0.10735205565866224 1104 98-99 0.25476265462020464 1104 100-101 0.2298712909694629 1104 102-103 0.22462380667323956 1104 104-105 0.015582396900395423 1104 106-107 0.16119175785098605 1104 108-109 0.17195227926439927 1104 110-111 0.17910734633525038 1104 112-113 0.2102126139874514 1104 114-115 0.08320281358391313 1104 116-117 0.12461187915497263 1104 118-119 0.08704341741999855 1104 120-121 0.15953126452959765 1104 122-123 0.005536846123590067 1104 124-125 0.07515264546425726 1105 1 -0.2510658524640377 1105 2 -0.29830957863373797 1105 3 -0.36721710260127693 1105 4 -0.65965222026513 1105 5 -0.524535293441815 1105 6 -0.3464464874456965 1105 7 -0.808855882014953 1105 8 -0.5810986364012436 1105 9 -0.8653841141930059 1105 10-11 -0.6213957575747742 1105 12-13 -0.8326354634184483 1105 14-15 -0.7817241428766266 1105 16-17 -0.7784519522664795 1105 18-19 -1.0924938534413329 1105 20-21 -1.0098471128975604 1105 22-23 -1.0021105147748415 1105 24-25 -0.7144677103173294 1105 26-27 -0.8333438494045922 1105 28-29 -0.6278339525640106 1105 30-31 -0.6925543663827511 1105 32-33 -0.7424534795680557 1105 34-35 -0.6686037505385727 1105 36-37 -0.7266316052555055 1105 38-39 -0.7307554340761016 1105 40-41 -0.7575434781165882 1105 42-43 -0.7245228189801622 1105 44-45 -0.8526983221477167 1105 46-47 -0.7341704796404613 1105 48-49 -0.9858702560261534 1105 50-51 -0.9512417119723864 1105 52-53 -0.8868997090764523 1105 54-55 -0.950312022604173 1105 56-57 -1.1963196069412163 1105 58-59 -1.2420517627441114 1105 60-61 -1.0528190274360298 1105 62-63 -0.9572854672876403 1105 64-65 -1.1181327375672936 1105 66-67 -1.0037323153775617 1105 68-69 -1.048031882971486 1105 70-71 -1.043965416734519 1105 72-73 -1.0792897300596067 1105 74-75 -1.0247612615173836 1105 76-77 -1.057166330402854 1105 78-79 -1.0664163048301205 1105 80-81 -1.0563058381968276 1105 82-83 -1.1980541871460026 1105 84-85 -1.1871744750634114 1105 86-87 -1.2048107063853095 1105 88-89 -1.2331714922649653 1105 90-91 -1.1831365308731279 1105 92-93 -1.2510446042691576 1105 94-95 -1.1696056858365012 1105 96-97 -1.0564079859596127 1105 98-99 -1.0560133326039534 1105 100-101 -0.975135000164876 1105 102-103 -1.0096695295636913 1105 104-105 -1.0118203324840422 1105 106-107 -1.0527219326252037 1105 108-109 -1.0507296796980476 1105 110-111 -1.127349535197851 1105 112-113 -1.1268228099382185 1105 114-115 -1.1797272822349036 1105 116-117 -0.9041246916928749 1105 118-119 -0.8594607686040057 1105 120-121 -0.8655637074022664 1105 122-123 -0.7184426592964641 1105 124-125 -0.7147295167502214 1106 1 0.2032039267037682 1106 2 0.22069959000500106 1106 3 0.29186650920022217 1106 4 0.2884099980459851 1106 5 0.4536550004065134 1106 6 0.3131841507914501 1106 7 0.25362999637869166 1106 8 0.4023954354970627 1106 9 0.4009999221485785 1106 10-11 0.3624223680272536 1106 12-13 0.41558670066570613 1106 14-15 0.5594390018118673 1106 16-17 0.40726649560546946 1106 18-19 0.4540420421955318 1106 20-21 0.32117820491289706 1106 22-23 0.31204914559309316 1106 24-25 0.4356345598423772 1106 26-27 0.5679267978703919 1106 28-29 0.5116548155562413 1106 30-31 0.3302453386986457 1106 32-33 0.40862392467258246 1106 34-35 0.48357959845240117 1106 36-37 0.44946036653695387 1106 38-39 0.4663405690633553 1106 40-41 0.33129183125065254 1106 42-43 0.3326325246356845 1106 44-45 0.4425324397649746 1106 46-47 0.26838627435223117 1106 48-49 0.17296659928535263 1106 50-51 0.10822065617640675 1106 52-53 0.141194855145649 1106 54-55 0.3618289912974362 1106 56-57 0.3631843827841976 1106 58-59 0.26659957925443933 1106 60-61 0.37426888922568224 1106 62-63 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-0.28523274604120985 2216 20-21 -0.2379121415123251 2216 22-23 -0.331372488741728 2216 24-25 -0.46059614273414695 2216 26-27 -0.4707325948686574 2216 28-29 -0.36246232179367155 2216 30-31 -0.46833111711843145 2216 32-33 -0.39113557593750414 2216 34-35 -0.3731234378523922 2216 36-37 -0.30929575647139984 2216 38-39 -0.23370717713905265 2216 40-41 -0.27904787413759635 2216 42-43 -0.32907590522161456 2216 44-45 -0.20550112404694687 2216 46-47 -0.25862481337698995 2216 48-49 -0.2724718700067683 2216 50-51 -0.3367447720362904 2216 52-53 -0.49810911132590974 2216 54-55 -0.4058282617043858 2216 56-57 -0.408892046507205 2216 58-59 -0.3794224547337848 2216 60-61 -0.3922451074851381 2216 62-63 -0.48183187374356606 2216 64-65 -0.4759219090933513 2216 66-67 -0.4328101439056553 2216 68-69 -0.3749784988477103 2216 70-71 -0.35171306526948243 2216 72-73 -0.33265760570727565 2216 74-75 -0.3523532891030925 2216 76-77 -0.3565182106722098 2216 78-79 -0.32083579536107365 2216 80-81 -0.4753152440569828 2216 82-83 -0.44053852575696695 2216 84-85 -0.3621929798746635 2216 86-87 -0.4585071552668971 2216 88-89 -0.512125654493488 2216 90-91 -0.46228476795135265 2216 92-93 -0.4796674852426044 2216 94-95 -0.3401740092103367 2216 96-97 -0.30802377191762176 2216 98-99 -0.3719590638599328 2216 100-101 -0.45845268715373777 2216 102-103 -0.49070164669463523 2216 104-105 -0.3750156169165315 2216 106-107 -0.3730402153787189 2216 108-109 -0.36029441043047683 2216 110-111 -0.2827935604004992 2216 112-113 -0.28005077115935606 2216 114-115 -0.5312482874523567 2216 116-117 -0.5601166398432973 2216 118-119 -0.6240272144165004 2216 120-121 -0.6115278532021264 2216 122-123 -0.6289254092016812 2216 124-125 -0.6138999596019481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15069.0 3 3345.0 4 3185.0 5 1935.0 6 2254.0 7 2280.0 8 2283.0 9 2204.0 10 2721.0 11 3462.0 12 3609.0 13 3019.0 14 2826.0 15 2770.0 16 2437.0 17 2415.0 18 1898.0 19 1462.0 20 1276.0 21 1042.0 22 1248.0 23 2208.0 24 5243.0 25 7202.0 26 8242.0 27 4159.0 28 3555.0 29 4162.0 30 4271.0 31 5428.0 32 7022.0 33 9805.0 34 15446.0 35 30754.0 36 72553.0 37 142702.0 38 64159.0 39 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.886954916729486 20.029621129739215 12.468227335499323 21.615196618031973 2 15.098655417640156 23.330074144685703 38.293759062066385 23.277511375607755 3 18.806954982914895 31.273412819275826 25.337755970894182 24.5818762269151 4 12.143435655989391 19.7290778500672 32.86599855816727 35.261487935776145 5 13.157186843480098 43.898713915720904 28.238053421962363 14.706045818836632 6 29.03534224949694 39.54240736417303 16.345039967092465 15.077210419237568 7 28.299839460658966 38.563906664828544 16.72143978440589 16.414814090106596 8 26.50432731884219 38.49823125013906 17.017376020646537 17.980065410372216 9 26.815465924895687 22.922726008344924 16.48289290681502 33.77891515994437 10-11 23.837431024150302 33.56882130376725 22.04025087585292 20.553496796229524 12-13 24.525312937723726 30.890566289436823 23.20374858260889 21.380372190230563 14-15 22.49344240430356 30.623527319610545 22.025852487440538 24.85717778864536 16-17 22.064316459748852 33.52680000711465 22.619148198214223 21.789735334922273 18-19 22.412343806637118 31.224773896278045 25.041195686739325 21.321686610345512 20-21 24.659840464621183 29.596663207542967 24.31678595447318 21.42671037336267 22-23 23.85863887742376 29.934105495732933 24.171145483005716 22.03611014383759 24-25 22.885896934000037 29.899171663967827 24.819162944641175 22.395768457390965 26-27 22.55149540064822 29.94836010545831 24.800709576166067 22.6994349177274 28-29 22.651914666607393 29.918034153362267 24.878162631353742 22.551888548676597 30-31 22.820923755393093 29.68962903523376 24.956043931617472 22.533403277755674 32-33 22.344278783904688 29.826232405128007 24.748573238567847 23.08091557239946 34-35 22.80851309762899 29.577446004025592 24.942900361547686 22.67114053679773 36-37 23.104767366390156 29.39116499070888 24.514772434273112 22.98929520862785 38-39 22.923427788990743 29.458561914838754 24.582773919924602 23.035236376245898 40-41 22.707626445219205 29.544653842007516 24.895168469520783 22.852551243252496 42-43 22.76207613625881 29.431084248701556 24.702993901497006 23.103845713542633 44-45 22.607581318117052 29.53304184106929 24.97115827051875 22.888218570294907 46-47 22.755540636521573 29.737426236924385 24.71036634503057 22.796666781523466 48-49 22.66870002967791 29.649678378141054 24.654897022093927 23.02672457008711 50-51 22.89092752063948 29.760195258143536 24.5948793640773 22.753997857139684 52-53 23.02529134225838 29.528572936985604 24.741390329159792 22.70474539159623 54-55 22.666226515548114 29.666296539589705 24.73426987710225 22.93320706775993 56-57 22.780645476816098 29.881757432675766 24.31108532404999 23.026511766458146 58-59 22.505040714747786 29.424102508241873 24.681051136300482 23.389805640709856 60-61 22.45267458609713 29.853466825529495 24.868849252218446 22.825009336154928 62-63 22.46651231188706 30.007814071776085 24.6891322156506 22.83654140068626 64-65 22.11951597752197 30.007503793584533 24.722053927263225 23.150926301630268 66-67 22.392351943973544 30.29775171608815 24.208098268626852 23.101798071311453 68-69 22.71451853531749 29.921758792964294 24.555821621609603 22.80790105010861 70-71 22.341954581985974 29.96557354999283 24.742579749421687 22.94989211859951 72-73 22.2496643221085 30.331832045456565 24.4056722894566 23.01283134297833 74-75 22.190842647176506 30.774693910213664 24.173868779286366 22.860594663323464 76-77 22.33133048355151 30.429756879377763 24.491896349745144 22.747016287325582 78-79 22.547662797173412 30.36935263522384 24.51720262649775 22.565781941105 80-81 22.326609192080422 30.844311749981102 24.33529262686129 22.493786431077183 82-83 22.418145540036573 30.769051205237798 24.099197990251387 22.71360526447425 84-85 22.50447950985492 30.355070627309228 24.334853921294012 22.80559594154184 86-87 22.483578465974716 30.497309302886567 24.252624277302434 22.766487953836286 88-89 22.397430752487598 30.38897775920086 24.312916382732507 22.900675105579044 90-91 22.390117066913007 30.627821483796165 24.14139628778607 22.840665161504763 92-93 22.347648116258465 30.1463125046553 24.55739939278254 22.9486399863037 94-95 22.198877449950476 30.15132970711204 24.725671134411417 22.924121708526066 96-97 22.43738459879566 30.254263383204812 24.36206269821772 22.94628931978181 98-99 22.096390658646158 30.615534608744372 24.030740600337733 23.25733413227174 100-101 21.859264084373965 30.855789260727377 24.354000715862583 22.930945939036075 102-103 21.953469964915715 30.66362217469218 24.668944847856924 22.713963012535185 104-105 21.48915498719408 31.213649864826408 24.488541370233353 22.80865377774616 106-107 21.36795085302377 31.77566353087139 24.062207478326993 22.794178137777845 108-109 21.441307247665627 31.831258337038683 23.815473543797243 22.911960871498444 110-111 21.403028642379873 32.31341101876256 23.87631987603531 22.407240462822266 112-113 21.297953466656292 32.56189068110319 23.778583100814835 22.361572751425683 114-115 20.887894460135225 32.90662558534766 23.658549327037754 22.546930627479355 116-117 21.065185568855156 32.85554072333562 23.847801416705476 22.231472291103742 118-119 20.901900405204856 33.02413998187973 23.55624232250749 22.51771729040793 120-121 20.921817890777152 33.04731478270149 23.7349820807655 22.295885245755855 122-123 21.04688076616973 32.97104187878761 23.601002174193347 22.381075180849315 124-125 21.077250051132474 33.12539460930344 23.432233911056173 22.36512142850791 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3699.0 1 3346.5 2 2851.0 3 2283.5 4 1466.5 5 962.0 6 776.0 7 716.0 8 743.0 9 723.0 10 606.0 11 496.5 12 460.5 13 454.0 14 465.5 15 480.0 16 494.5 17 569.5 18 650.5 19 712.0 20 845.5 21 1009.5 22 1177.0 23 1427.5 24 1761.5 25 2092.5 26 2508.0 27 3049.0 28 3572.5 29 4221.5 30 4868.5 31 5551.0 32 6624.0 33 7281.5 34 7733.0 35 8037.5 36 8647.0 37 9654.0 38 10029.5 39 10086.5 40 10103.5 41 10255.5 42 10512.0 43 11085.5 44 11865.0 45 12453.5 46 12686.0 47 13128.5 48 14126.5 49 14866.5 50 15249.0 51 15264.0 52 15342.5 53 16023.5 54 16473.0 55 16218.0 56 15098.0 57 13806.0 58 13552.5 59 13055.0 60 11586.5 61 10026.5 62 8521.5 63 6370.5 64 4704.5 65 4017.0 66 3012.0 67 2229.5 68 1617.5 69 1059.0 70 809.5 71 677.5 72 602.5 73 584.5 74 452.0 75 346.0 76 262.0 77 163.0 78 139.5 79 91.0 80 50.0 81 34.0 82 20.0 83 14.0 84 15.5 85 12.0 86 5.0 87 5.5 88 7.0 89 5.5 90 4.0 91 3.0 92 3.5 93 4.0 94 2.5 95 1.5 96 1.5 97 0.5 98 0.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05223220437683647 2 0.20603937641415915 3 0.02378232284392128 4 0.10802064394528732 5 0.022004205248114084 6 0.03756273421142707 7 0.04000764590566197 8 0.09868552656729954 9 0.11980067301751002 10-11 0.046564454540201014 12-13 0.032006116724529576 14-15 0.012002293771698591 16-17 0.03067252852767418 18-19 0.05134314557893287 20-21 0.19114764154927386 22-23 0.10746498219659757 24-25 0.07512546842285414 26-27 0.01511399956436119 28-29 0.007001338033490845 30-31 0.007334735082704695 32-33 0.007001338033490845 34-35 0.009446249727725743 36-37 0.004889823388469796 38-39 0.008446058580084195 40-41 0.0056677498366354455 42-43 0.011002102624057042 44-45 0.010001911476415493 46-47 0.01744777890885814 48-49 0.018781367105713535 50-51 0.010668705574843191 52-53 0.012557955520388343 54-55 0.01522513191409914 56-57 0.01689211716016839 58-59 0.017114381859644288 60-61 0.011113234973794992 62-63 0.018781367105713535 64-65 0.03145045497583983 66-67 0.028116484483701333 68-69 0.03356196962086088 70-71 0.025449308089990533 72-73 0.01733664655912019 74-75 0.010224176175891391 76-77 0.012224558471174492 78-79 0.025227043390514632 80-81 0.020448352351782783 82-83 0.02367119049418333 84-85 0.019559293553879184 86-87 0.02744969038527363 88-89 0.02945007268055673 90-91 0.028561013882653127 92-93 0.035006690167454224 94-95 0.02989460207950853 96-97 0.027671955084749532 98-99 0.03345083727112293 100-101 0.02378232284392128 102-103 0.031117057926625977 104-105 0.027116293336059784 106-107 0.03578461661561987 108-109 0.02533817574025258 110-111 0.024115719893135133 112-113 0.02867214623239108 114-115 0.01655872011095454 116-117 0.015002867214623239 118-119 0.03145045497583983 120-121 0.02622723453815618 122-123 0.020448352351782783 124-125 0.022670999346541782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 449914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.15846292741384 #Duplication Level Percentage of deduplicated Percentage of total 1 77.94295952792505 45.33042722157746 2 11.607368901652466 13.501334679031421 3 4.280430552170224 7.4682978474528445 4 2.049421091934196 4.767647223916597 5 1.1492402234905923 3.3419022466285195 6 0.7067760626935878 2.4663005664089126 7 0.4848984174476688 1.974066264428432 8 0.32418147094957545 1.5083116847980296 9 0.24402282163586742 1.2772793002997263 >10 1.1180155218067727 11.251278458501018 >50 0.057832630816501224 2.269108317179425 >100 0.0333207872587465 3.4104562105892287 >500 0.001148992664094707 0.4532870739043224 >1k 3.8299755469823563E-4 0.9803029052839916 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4401 0.9781869423934352 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 801 0.17803402428019577 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 620 0.1378041136750579 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 614 0.13647052547820251 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2226469947589985E-4 0.0 22-23 0.0 0.0 0.0 7.779264481656495E-4 0.0 24-25 0.0 0.0 0.0 0.001666985246069249 0.0 26-27 0.0 0.0 0.0 0.0032228381424005475 0.0 28-29 0.0 0.0 0.0 0.005556617486897496 0.0 30-31 0.0 0.0 0.0 0.01278022021986424 0.0 32-33 0.0 0.0 0.0 0.02600496983868028 0.0 34-35 0.0 0.0 0.0 0.04056330765435172 0.0 36-37 0.0 0.0 0.0 0.0674573362909356 0.0 38-39 0.0 0.0 0.0 0.11635557017563355 0.0 40-41 0.0 0.0 0.0 0.18047893597443065 0.0 42-43 0.0 0.0 0.0 0.2303773610067702 0.0 44-45 0.0 0.0 0.0 0.28905524166840774 0.0 46-47 0.0 0.0 0.0 0.36306938659388244 0.0 48-49 0.0 0.0 0.0 0.4476411047444623 0.0 50-51 0.0 0.0 0.0 0.5444373813662167 0.0 52-53 0.0 0.0 0.0 0.6414559226874469 0.0 54-55 0.0 0.0 0.0 0.7354738905657525 0.0 56-57 0.0 0.0 0.0 0.8422720786639224 0.0 58-59 0.0 0.0 0.0 0.9704076779117787 0.0 60-61 0.0 0.0 0.0 1.1325497761794476 0.0 62-63 0.0 0.0 0.0 1.2832452424241079 0.0 64-65 0.0 0.0 0.0 1.4226052089954968 0.0 66-67 0.0 0.0 0.0 1.577634836879937 0.0 68-69 0.0 0.0 0.0 1.764003787390479 0.0 70-71 0.0 0.0 0.0 1.9891579279595657 0.0 72-73 0.0 0.0 0.0 2.214312068528652 0.0 74-75 0.0 0.0 0.0 2.4244633418831154 0.0 76-77 0.0 0.0 0.0 2.64972861480194 0.0 78-79 0.0 0.0 0.0 2.925003445102842 0.0 80-81 0.0 0.0 0.0 3.2190596425094573 0.0 82-83 0.0 0.0 0.0 3.530897015874145 0.0 84-85 0.0 0.0 0.0 3.834510595358224 0.0 86-87 0.0 0.0 0.0 4.146347968722912 0.0 88-89 0.0 0.0 0.0 4.495303546900074 0.0 90-91 0.0 0.0 0.0 4.894379814809053 0.0 92-93 0.0 0.0 0.0 5.3454660223953905 0.0 94-95 0.0 0.0 0.0 5.778882186373396 0.0 96-97 0.0 0.0 0.0 6.184626395266651 0.0 98-99 0.0 0.0 0.0 6.630378250065569 0.0 100-101 0.0 0.0 0.0 7.164035793507203 0.0 102-103 0.0 0.0 0.0 7.727921336077562 0.0 104-105 0.0 0.0 0.0 8.298030290233244 0.0 106-107 0.0 0.0 0.0 8.836911054112564 0.0 108-109 0.0 0.0 0.0 9.398351684988686 0.0 110-111 0.0 0.0 0.0 10.03458438723845 0.0 112-113 0.0 0.0 0.0 10.720826646870291 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1935 0.0 49.962315 1 GGTATCA 1070 0.0 44.06074 1 ACGCAAA 165 5.456968E-12 39.784805 1 ATCAACG 2315 0.0 38.873486 3 TCAACGC 2320 0.0 38.532818 4 GAACAAA 695 0.0 37.781254 1 TACAAGG 285 0.0 37.68246 2 GTACATG 4910 0.0 37.434994 1 TATCAAC 2610 0.0 36.80417 2 CGATAGC 65 0.0019221411 36.675343 4 AACGCAG 2440 0.0 36.62144 6 CAACGCA 2445 0.0 36.54655 5 TACATGG 4970 0.0 35.894375 2 GTATAGA 105 1.4518111E-5 34.10126 1 GTACAAG 435 0.0 32.925354 1 TCGACCG 55 2.0043233E-5 32.536686 18-19 ACGAATC 55 2.0436308E-5 32.44443 72-73 ACATGGG 5305 0.0 32.017467 3 GAGAACA 845 0.0 31.780764 1 ATGGGAT 1400 0.0 31.061956 5 >>END_MODULE