FastQCFastQC Report
Fri 27 May 2016
SRR939995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449914
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94442.099067821850398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68131.5142893975293055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46051.0235289410865187No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.38918548878230064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.3722933716221322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.3665144894357588No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.26493952177527264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.18136799477233426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.15425170143627448No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5530.12291237881017261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4630.10290855585734161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4620.10268629115786573No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.10201949705943804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG750.087.370562
GGTATCA26350.074.864231
GTATCAA54200.061.686571
TATCACG507.3923093E-659.570832
ATAACGC1100.059.570833
TAACGCA1300.050.4060864
TATCAAC71350.046.8384552
ATCAACG71700.046.526733
TCAACGC72400.045.9946064
CAACGCA73950.044.9050255
AACGCAG75450.044.0073856
GTATAAC2200.040.6345631
GATATAG1551.1823431E-1038.449911
ACGCAGA86250.038.4193657
GTCCAAG3300.037.9255941
CGCAGAG87300.037.8209888
CTTACTA1304.1984094E-836.6100859
GTGGTAT9750.036.0640451
GCAGAGT92100.035.7852639
CCAAGAC2950.034.3289533
Produced by FastQC (version 0.11.5)