##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 449914 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.065305814000006 34.0 33.0 34.0 31.0 34.0 2 33.086605440150784 34.0 33.0 34.0 31.0 34.0 3 33.13879319158772 34.0 33.0 34.0 31.0 34.0 4 36.40526189449539 37.0 37.0 37.0 35.0 37.0 5 36.44061976288802 37.0 37.0 37.0 35.0 37.0 6 36.55367025698244 37.0 37.0 37.0 35.0 37.0 7 36.53487110870078 37.0 37.0 37.0 35.0 37.0 8 36.55114088470241 37.0 37.0 37.0 35.0 37.0 9 38.39288841867557 39.0 39.0 39.0 37.0 39.0 10-11 38.38319990042541 39.0 39.0 39.0 37.0 39.0 12-13 38.36176913810195 39.0 39.0 39.0 37.0 39.0 14-15 39.812653084811764 41.0 40.0 41.0 37.5 41.0 16-17 39.84322003760718 41.0 40.0 41.0 37.5 41.0 18-19 39.98204101228235 41.0 40.0 41.0 38.0 41.0 20-21 39.99329316269331 41.0 40.0 41.0 38.0 41.0 22-23 39.90530968140578 41.0 40.0 41.0 37.5 41.0 24-25 39.83741892895087 41.0 40.0 41.0 37.0 41.0 26-27 39.67917424218851 41.0 40.0 41.0 36.5 41.0 28-29 39.52888774299088 41.0 40.0 41.0 35.0 41.0 30-31 39.13751516956574 41.0 38.5 41.0 35.0 41.0 32-33 38.694377147632665 41.0 37.5 41.0 35.0 41.0 34-35 38.4483445725183 41.0 37.0 41.0 35.0 41.0 36-37 38.24420111399067 41.0 36.5 41.0 35.0 41.0 38-39 38.04021435207618 41.0 36.0 41.0 35.0 41.0 40-41 37.75363736180692 40.5 35.5 41.0 35.0 41.0 42-43 37.38482243273158 40.0 35.0 41.0 33.5 41.0 44-45 36.84254546424428 40.0 35.0 41.0 32.5 41.0 46-47 36.0071680365581 40.0 35.0 41.0 27.5 41.0 48-49 35.26604973394915 40.0 35.0 41.0 21.5 41.0 50-51 35.09709299999555 40.0 35.0 41.0 18.0 41.0 52-53 34.93868050338509 40.0 35.0 41.0 17.0 41.0 54-55 34.74871197606654 40.0 35.0 41.0 15.0 41.0 56-57 34.53568348617736 39.0 35.0 41.0 11.5 41.0 58-59 34.28903634916895 39.0 35.0 41.0 9.0 41.0 60-61 34.029671226056536 38.5 35.0 41.0 7.0 41.0 62-63 33.736849709055505 37.0 35.0 41.0 6.0 41.0 64-65 33.411241926234794 37.0 35.0 40.5 2.0 41.0 66-67 33.071969309690296 36.0 35.0 39.5 2.0 41.0 68-69 32.714559893668564 35.5 35.0 39.0 2.0 41.0 70-71 32.35987877683291 35.0 35.0 39.0 2.0 41.0 72-73 32.03433545077504 35.0 34.0 37.5 2.0 41.0 74-75 31.71685366536716 35.0 34.0 37.0 2.0 39.5 76-77 31.408433611756912 35.0 34.0 36.5 2.0 39.0 78-79 31.13309543601666 35.0 34.0 36.0 2.0 39.0 80-81 30.876235236067338 35.0 34.0 36.0 2.0 37.0 82-83 30.6413914659246 35.0 34.0 35.5 2.0 37.0 84-85 30.43766364238499 35.0 34.0 35.0 2.0 37.0 86-87 30.256889094360254 35.0 33.0 35.0 2.0 36.0 88-89 30.169401263352555 35.0 33.5 35.0 2.0 36.0 90-91 30.172191796654474 35.0 34.0 35.0 2.0 36.0 92-93 30.085698600176926 35.0 34.0 35.0 2.0 36.0 94-95 30.005512164547003 35.0 34.0 35.0 2.0 35.5 96-97 29.929408731446454 35.0 34.0 35.0 2.0 35.0 98-99 29.858564081135505 35.0 34.0 35.0 2.0 35.0 100-101 29.780588067941874 35.0 34.0 35.0 2.0 35.0 102-103 29.29893601888361 34.5 32.0 35.0 2.0 35.0 104-105 29.64729370501918 35.0 33.0 35.0 2.0 35.0 106-107 29.6692112270345 35.0 33.5 35.0 2.0 35.0 108-109 29.627933338371335 35.0 34.0 35.0 2.0 35.0 110-111 29.556007370297436 35.0 33.0 35.0 2.0 35.0 112-113 29.493158692550132 35.0 33.0 35.0 2.0 35.0 114-115 29.398526162777777 35.0 33.0 35.0 2.0 35.0 116-117 29.313046493329836 35.0 33.0 35.0 2.0 35.0 118-119 29.220761078783944 35.0 33.0 35.0 2.0 35.0 120-121 29.11895273318901 35.0 33.0 35.0 2.0 35.0 122-123 28.979153793836154 35.0 33.0 35.0 2.0 35.0 124-125 27.975428637472938 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18161983658679048 1101 2 0.24500606493024435 1101 3 0.2303184233260822 1101 4 0.06755697153604245 1101 5 0.17480394894018758 1101 6 0.1301082964468705 1101 7 0.20166350032115332 1101 8 0.18022973671914144 1101 9 0.19996517345968812 1101 10-11 0.24817836271787286 1101 12-13 0.21769043853255 1101 14-15 0.29080410766542286 1101 16-17 0.17289862035035952 1101 18-19 0.19188535901970027 1101 20-21 0.19226992518999708 1101 22-23 0.17875178842494677 1101 24-25 0.20623515987470142 1101 26-27 0.25314761660439444 1101 28-29 0.31898987411037183 1101 30-31 0.3310167358337779 1101 32-33 0.44740667646308196 1101 34-35 0.3124613111935943 1101 36-37 0.20595074779522093 1101 38-39 0.1924864816175642 1101 40-41 0.37189018286015596 1101 42-43 0.3746935743574227 1101 44-45 0.4386386599359966 1101 46-47 0.44933877740625405 1101 48-49 0.7922996421421047 1101 50-51 0.6250151106330293 1101 52-53 0.6616719352533664 1101 54-55 0.7074278592698846 1101 56-57 0.5825663610269487 1101 58-59 0.39074484954007005 1101 60-61 0.49334927272220597 1101 62-63 0.5410474120157645 1101 64-65 0.6854024909992731 1101 66-67 0.6778586424441997 1101 68-69 0.7827043009584074 1101 70-71 0.7770870780821824 1101 72-73 0.7043393069093042 1101 74-75 0.6672046243328964 1101 76-77 0.6632778194691049 1101 78-79 0.6860837536986928 1101 80-81 0.6647372611535438 1101 82-83 0.5111367890520278 1101 84-85 0.64328954320181 1101 86-87 0.5075500615682067 1101 88-89 0.4663197075328007 1101 90-91 0.46284177524891135 1101 92-93 0.4934874971916905 1101 94-95 0.7407671957776856 1101 96-97 0.7568533307604817 1101 98-99 0.6987770193715548 1101 100-101 0.554837082519839 1101 102-103 0.5449108389332835 1101 104-105 0.5765693504853573 1101 106-107 0.6394947974480587 1101 108-109 0.6262056954207509 1101 110-111 0.7937062383437876 1101 112-113 0.8468628694065536 1101 114-115 0.6927053104231007 1101 116-117 0.6621805501840186 1101 118-119 0.683393909594237 1101 120-121 0.6643355815948517 1101 122-123 0.4595049351521858 1101 124-125 0.4256128929088838 1102 1 0.14639256385951427 1102 2 0.2166943766185554 1102 3 0.1628508908585502 1102 4 0.06538164686071468 1102 5 0.0445766762129125 1102 6 -0.026222872384295215 1102 7 0.028189474347129817 1102 8 0.09009986658927716 1102 9 0.04931582281033542 1102 10-11 0.11366537570488333 1102 12-13 0.10762550346760946 1102 14-15 0.16043073104204097 1102 16-17 0.21806095801269265 1102 18-19 0.17108990447425043 1102 20-21 0.19259460051467414 1102 22-23 0.2681349053080595 1102 24-25 0.2082806144201541 1102 26-27 0.23694631790309018 1102 28-29 0.29647364034414636 1102 30-31 -0.0062397576727164505 1102 32-33 0.07170862451503268 1102 34-35 0.31127624625852945 1102 36-37 0.2725903581848357 1102 38-39 0.2813988192799002 1102 40-41 0.08249083221080866 1102 42-43 0.0585247431885918 1102 44-45 -0.10722172967439292 1102 46-47 -0.31053135246386887 1102 48-49 -0.5161906175981557 1102 50-51 -0.45272839586047553 1102 52-53 -0.3019319608505242 1102 54-55 0.060836950178973837 1102 56-57 0.13881636102695438 1102 58-59 0.05368316122838479 1102 60-61 0.12065446752739462 1102 62-63 0.22208637305472223 1102 64-65 0.12593820528498156 1102 66-67 0.1519333177688793 1102 68-69 0.02961988537398952 1102 70-71 -0.03818889594379016 1102 72-73 0.026368527688518384 1102 74-75 0.11976955939783807 1102 76-77 0.0612972999885848 1102 78-79 0.04760972772466587 1102 80-81 -0.11966208949580803 1102 82-83 -0.23672035380511147 1102 84-85 -0.25184032692806113 1102 86-87 -0.27158954882140307 1102 88-89 -0.12066081194771527 1102 90-91 -0.1694958870887504 1102 92-93 -0.1852138015096081 1102 94-95 -0.23661916785867732 1102 96-97 -0.24983498092782952 1102 98-99 -0.14562233127779933 1102 100-101 -0.18886421618146088 1102 102-103 -0.20583591431346804 1102 104-105 -0.22724558457957755 1102 106-107 -0.22086234540908478 1102 108-109 -0.2969274214623674 1102 110-111 -0.29509246295491565 1102 112-113 -0.24487414358045712 1102 114-115 -0.10804144282365158 1102 116-117 -0.16582269656922932 1102 118-119 -0.06780738910706319 1102 120-121 -0.16503130152203127 1102 122-123 -0.16984571419846262 1102 124-125 -0.17596178241578997 1103 1 0.23738385105333037 1103 2 0.19147984431541687 1103 3 0.21354627142734728 1103 4 -0.016778471501936565 1103 5 0.07799110988050728 1103 6 -0.06715662217880691 1103 7 0.10277109525786443 1103 8 0.08475053237918218 1103 9 0.18604112282677931 1103 10-11 0.18590440250087426 1103 12-13 0.19992371158860323 1103 14-15 0.2390975073037538 1103 16-17 0.34421417188742254 1103 18-19 0.24241655250975924 1103 20-21 0.22396070276685265 1103 22-23 0.1576170687142735 1103 24-25 0.3227586680121348 1103 26-27 0.32405629653206347 1103 28-29 0.27564674571978287 1103 30-31 0.38075679008332486 1103 32-33 0.4434396782713961 1103 34-35 0.6451805697831077 1103 36-37 0.7407721763666544 1103 38-39 0.8917292483445038 1103 40-41 0.995635662064501 1103 42-43 0.701682724447835 1103 44-45 0.9105080089414201 1103 46-47 1.2164273850011895 1103 48-49 1.3774375264097358 1103 50-51 1.3773998303436983 1103 52-53 1.2343889334450466 1103 54-55 1.0294215301559575 1103 56-57 1.0873697064157426 1103 58-59 1.1070762238619523 1103 60-61 1.0241132871887473 1103 62-63 0.806508533173087 1103 64-65 0.7632618942542422 1103 66-67 0.581237937200072 1103 68-69 0.6025279899276654 1103 70-71 0.6726906043027938 1103 72-73 0.6798931043776548 1103 74-75 0.7321006460327197 1103 76-77 0.540832069017025 1103 78-79 0.7213233558686767 1103 80-81 0.8691789428895298 1103 82-83 0.8462633713305117 1103 84-85 0.8105137746665463 1103 86-87 0.6671002424000321 1103 88-89 0.7101036135002516 1103 90-91 0.847742317744391 1103 92-93 0.7620227593978406 1103 94-95 0.7185361831194612 1103 96-97 0.784475392243305 1103 98-99 0.8685894063516599 1103 100-101 0.9416815671491356 1103 102-103 1.012383714159327 1103 104-105 1.0389224246264064 1103 106-107 0.9953944357844051 1103 108-109 1.0338797460536604 1103 110-111 1.0044883359929742 1103 112-113 0.9933027428242731 1103 114-115 1.0675018746184008 1103 116-117 1.0076936604914337 1103 118-119 0.9917799855436016 1103 120-121 0.9735467027521771 1103 122-123 0.9134832353330218 1103 124-125 0.7834677753681945 1104 1 0.2923837254756734 1104 2 0.27020447762865984 1104 3 0.21429659792925548 1104 4 0.1479637175677837 1104 5 0.21265117116240617 1104 6 0.19221147105004377 1104 7 0.1475960400036982 1104 8 0.19084481608422266 1104 9 0.12734612584063854 1104 10-11 0.22312875954327183 1104 12-13 0.27577575599286064 1104 14-15 0.31498565528446676 1104 16-17 0.330810326699563 1104 18-19 0.3554270256863745 1104 20-21 0.3172947267772983 1104 22-23 0.2927150820757376 1104 24-25 0.24790182654137283 1104 26-27 0.36366348962026507 1104 28-29 0.3679730090310116 1104 30-31 0.32578360091314806 1104 32-33 0.38406342249482606 1104 34-35 0.4790287715110537 1104 36-37 0.6400777319222115 1104 38-39 0.6146342990778777 1104 40-41 0.40638919079666636 1104 42-43 0.4247431775320223 1104 44-45 0.4040156440629801 1104 46-47 0.2595570313745128 1104 48-49 0.4332222611897194 1104 50-51 0.40438018999810765 1104 52-53 0.24825427652321252 1104 54-55 0.11020563704766317 1104 56-57 0.07909413880472016 1104 58-59 -0.06252895998374441 1104 60-61 -0.11173505267462502 1104 62-63 0.06775872153956897 1104 64-65 0.16322987195164984 1104 66-67 0.16468899958705663 1104 68-69 0.10616660254570576 1104 70-71 5.989828440817746E-4 1104 72-73 0.16981549738548551 1104 74-75 0.1794318068725822 1104 76-77 0.26977583534212 1104 78-79 0.25632184893678556 1104 80-81 0.20357654686782922 1104 82-83 0.30473797952822324 1104 84-85 0.27248101145577763 1104 86-87 0.35397863299677823 1104 88-89 0.4570191122947094 1104 90-91 0.3275243149314484 1104 92-93 0.3309130924297854 1104 94-95 0.3806977513332441 1104 96-97 0.401099362506514 1104 98-99 0.27181769397472877 1104 100-101 0.486186288869046 1104 102-103 0.4003919897269341 1104 104-105 0.48482827905678505 1104 106-107 0.4775404323686949 1104 108-109 0.37543684621439866 1104 110-111 0.11513480977235702 1104 112-113 0.17989858369226752 1104 114-115 0.22353368343897273 1104 116-117 0.21833134383481223 1104 118-119 0.2442570048323276 1104 120-121 0.09913220857898253 1104 122-123 0.15486703832679183 1104 124-125 0.02263670243269189 1105 1 0.19089684007111174 1105 2 0.11991895691630816 1105 3 0.1093253919671966 1105 4 -0.017329788045842065 1105 5 0.09479523813879354 1105 6 0.16177205951307627 1105 7 0.09604503342220028 1105 8 0.1534004684264616 1105 9 0.18324043478372687 1105 10-11 0.2566713940767542 1105 12-13 0.266732250379242 1105 14-15 0.3599460937281336 1105 16-17 0.3358341604200419 1105 18-19 0.2652520837583765 1105 20-21 0.340266440869442 1105 22-23 0.28393471525421177 1105 24-25 0.31359578705239954 1105 26-27 0.19840022984480754 1105 28-29 0.3014593863054955 1105 30-31 0.2874409518616474 1105 32-33 0.28693193778712356 1105 34-35 0.13216949934690803 1105 36-37 -0.1033044786856081 1105 38-39 -0.192291392946899 1105 40-41 -0.1740375174882729 1105 42-43 -0.3895050319143536 1105 44-45 -0.4879946153253343 1105 46-47 -0.6959573898411264 1105 48-49 -0.6966594170913467 1105 50-51 -0.5921233911091335 1105 52-53 -0.7084064619591715 1105 54-55 -0.9208386912527615 1105 56-57 -1.213274231307551 1105 58-59 -1.290897136522652 1105 60-61 -1.137356302190689 1105 62-63 -1.2544229712595012 1105 64-65 -1.237267369627908 1105 66-67 -1.1568756781132912 1105 68-69 -1.3274437826652878 1105 70-71 -1.2177517720920363 1105 72-73 -1.2293932714879148 1105 74-75 -1.1616716822873059 1105 76-77 -1.1356333303566792 1105 78-79 -1.0229319257438156 1105 80-81 -0.9060162231670148 1105 82-83 -0.8271036290664391 1105 84-85 -0.923696519515957 1105 86-87 -1.013290530766291 1105 88-89 -1.039124543338275 1105 90-91 -0.8650327892110212 1105 92-93 -0.9525055341671909 1105 94-95 -1.0404392502153428 1105 96-97 -0.92006304554614 1105 98-99 -0.9228475450883771 1105 100-101 -1.0459468896055988 1105 102-103 -1.0100107638541722 1105 104-105 -0.9214489421975713 1105 106-107 -1.0131619969770291 1105 108-109 -0.9493822836733301 1105 110-111 -1.0079052599140539 1105 112-113 -0.779378419792053 1105 114-115 -0.7542023550821249 1105 116-117 -0.791891749467549 1105 118-119 -0.9142977280364271 1105 120-121 -0.98372626509504 1105 122-123 -0.9796936711195876 1105 124-125 -1.009556967718293 1106 1 0.038573761501460524 1106 2 0.07279738623692822 1106 3 -0.003677188570549106 1106 4 0.037711774071389925 1106 5 0.047542856790016685 1106 6 0.05972189956730034 1106 7 0.07072127701545128 1106 8 0.07389136519305595 1106 9 -0.015003134682679331 1106 10-11 0.13393531054819618 1106 12-13 0.1349137442370818 1106 14-15 0.1792579106883352 1106 16-17 0.11251953697639294 1106 18-19 0.17505515424154794 1106 20-21 0.2209998130495805 1106 22-23 0.2964687557579566 1106 24-25 0.2563631462228244 1106 26-27 0.25241017145568634 1106 28-29 0.30465235582661165 1106 30-31 0.3045266090663503 1106 32-33 0.24032171790628354 1106 34-35 0.4542610430317211 1106 36-37 0.4958916302915739 1106 38-39 0.5905331661616913 1106 40-41 0.6364653900761539 1106 42-43 0.7863342374485995 1106 44-45 0.5781955833879664 1106 46-47 0.5160867052463374 1106 48-49 0.30653355242976943 1106 50-51 0.3642185479806628 1106 52-53 0.48480089673557814 1106 54-55 0.5975729104644216 1106 56-57 0.4939358149518682 1106 58-59 0.46121961794572286 1106 60-61 0.3618707256719844 1106 62-63 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110-111 -0.28169161509811147 2216 112-113 -0.32003664946835286 2216 114-115 -0.1939434682593557 2216 116-117 -0.32587922035632033 2216 118-119 -0.2406901022488448 2216 120-121 -0.27851693505947495 2216 122-123 -0.3617015785413713 2216 124-125 -0.5333758191562517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 1.0 4 20.0 5 59.0 6 172.0 7 353.0 8 542.0 9 950.0 10 1843.0 11 2701.0 12 3399.0 13 5995.0 14 11364.0 15 11759.0 16 4180.0 17 2914.0 18 2818.0 19 2301.0 20 1966.0 21 1743.0 22 1586.0 23 1697.0 24 1884.0 25 2161.0 26 2398.0 27 2773.0 28 3187.0 29 3624.0 30 4494.0 31 5249.0 32 6617.0 33 8654.0 34 12999.0 35 31126.0 36 63793.0 37 158951.0 38 83028.0 39 608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.78542735128238 17.69316784822677 14.01443659701798 19.506968203472873 2 16.508076221213113 20.72522621643428 40.448898757726695 22.31779880462591 3 19.393304927841605 28.600418303094155 29.20609095536248 22.80018581370176 4 11.970744757805145 16.837121928744878 36.15283771842942 35.039295595020555 5 12.155650675412751 38.76680193451554 31.196842514870198 17.880704875201513 6 29.080796786534385 37.56704908448685 16.443892170178522 16.908261958800242 7 26.541072293816153 36.70301435385429 18.977626835350755 17.7782865169788 8 28.307409860551125 31.97833363709509 18.23326235680597 21.480994145547815 9 29.89638019710433 14.502104846704036 19.373702529816807 36.22781242637482 10-11 25.490315927043834 27.617944762776887 23.879007988193298 23.01273132198598 12-13 28.396093475642008 25.52865658770343 26.32147477073396 19.753775165920597 14-15 24.607813937774775 24.139724480678527 28.68492645261094 22.567535128935752 16-17 20.23875674017701 28.19905581955663 28.49789070800197 23.06429673226439 18-19 20.045275319283242 22.867814737927695 36.601995047942495 20.484914894846572 20-21 21.850286943727024 21.581124392661707 37.219890912485496 19.34869775112577 22-23 20.944891690411946 22.05321461434852 37.23111527980903 19.770778415430502 24-25 20.139182154811806 22.278924416666296 37.650862164769265 19.931031263752626 26-27 20.0073791880226 22.46884960236845 37.374920540369935 20.14885066923901 28-29 19.98008503866303 22.340430260961565 37.61571681636228 20.06376788401313 30-31 20.12964714328421 22.1584815747916 37.69924663296389 20.0126246489603 32-33 19.909404707132268 22.32909473609342 37.27765145705948 20.483849099714835 34-35 20.260939627149725 22.23232295168505 37.28080460089462 20.225932820270607 36-37 20.695047031419477 22.000302281335017 36.63783139814008 20.666819289105426 38-39 20.48210445596546 22.075894221525775 36.52847005740132 20.91353126510744 40-41 20.51647648217215 22.30304013655943 36.398511715572305 20.781971665696112 42-43 20.811588146910033 22.31567342656721 35.62883967388888 21.24389875263387 44-45 20.890488219323622 22.57671247948203 35.128208832645605 21.404590468548747 46-47 21.560144338856247 23.196792272292342 33.58301095655054 21.660052432300876 48-49 21.868349371708373 23.750946021486495 32.10482973835907 22.275874868446056 50-51 22.117335882019535 24.33213678443227 31.262711016770204 22.287816316777985 52-53 22.180177056453136 24.51962935056333 30.8022884424322 22.49790515055133 54-55 21.710371315406945 24.710944758331593 30.778548789324184 22.80013513693728 56-57 22.07672414846953 24.68946601601874 30.38259831677634 22.851211518735393 58-59 21.974912676688703 23.957204631608427 30.96963049153328 23.09825220016959 60-61 22.224025053927512 24.050392356685066 31.364418844234233 22.361163745153192 62-63 22.35249403219282 24.039149704165684 31.52513591486373 22.083220348777765 64-65 22.20310728812429 23.938120957524838 31.63299326531973 22.22577848903114 66-67 22.411407576823443 24.39057459078063 31.07055498692521 22.12746284547072 68-69 22.785087256243187 24.14646913108274 31.11641093061842 21.952032682055652 70-71 22.426904906642545 24.314502551062933 31.091072557764264 22.167519984530255 72-73 22.492076263463684 24.553470218752917 30.663415674995665 22.291037842787734 74-75 22.221246727152298 25.043563881097274 30.266228657032237 22.46896073471819 76-77 22.145341025404505 24.72446685433779 30.76046814795716 22.36972397230054 78-79 22.09386321708786 24.772621190904847 30.637349692160655 22.496165899846634 80-81 21.773827887107313 25.048120307436534 30.464377636614998 22.713674168841155 82-83 21.98097858701885 24.791293447192132 30.023404472854814 23.204323492934208 84-85 21.87589600656127 24.577862831258486 30.006245651937153 23.53999551024309 86-87 21.72264415564787 24.5774183008715 29.958125237545925 23.741812305934705 88-89 21.657583449281418 24.579808585640812 30.025293722800356 23.737314242277414 90-91 21.63365098769205 24.859833856583226 30.00605673325369 23.500458422471034 92-93 21.59131826365273 24.36720677468827 30.30761707897135 23.733857882687648 94-95 21.513588243008062 24.26779963148267 30.456579190106485 23.76203293540278 96-97 21.828466646292334 24.244714249993056 30.301336370961018 23.625482732753593 98-99 21.657694581631155 24.294865240912706 29.911049667269747 24.13639051018639 100-101 21.63304724130305 24.355348305841012 30.184024262441554 23.82758019041438 102-103 21.95397342603253 23.914348075409965 30.30912574403108 23.82255275452642 104-105 21.646581346657364 24.042039145258872 30.276786230257336 24.03459327782643 106-107 21.720643768906434 24.12510863211332 30.041919215492776 24.112328383487476 108-109 21.78301336370961 24.03167282527158 29.74489484066346 24.440418970355346 110-111 21.65436061113902 24.39232831163289 29.76602195086172 24.187289126366373 112-113 21.403185511833424 24.47234125784598 29.555001867031777 24.569471363288823 114-115 20.922277443388428 24.57413515652089 29.70962952425829 24.793957875832394 116-117 20.98550711752671 24.44808467461862 29.900502433261728 24.665905774592947 118-119 20.7692376081598 24.66932495838084 29.674514850648904 24.886922582810456 120-121 20.754188578261623 24.839858284027613 29.54709122187796 24.858861915832804 122-123 20.851744341115598 24.80740678177066 29.173149415885774 25.167699461227972 124-125 20.60545105992832 24.88350512599672 29.03753507626483 25.47350873781013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 728.0 1 515.5 2 878.0 3 1289.5 4 674.5 5 387.0 6 1116.0 7 2816.5 8 4821.5 9 5092.0 10 3233.5 11 1557.5 12 1147.0 13 1192.5 14 1240.0 15 1333.5 16 1448.5 17 1542.5 18 1687.0 19 1739.0 20 1776.0 21 1888.5 22 1978.5 23 2209.0 24 2503.0 25 2721.5 26 3089.0 27 3472.5 28 3780.0 29 4349.5 30 4938.0 31 5525.5 32 6472.5 33 6926.0 34 7158.5 35 7261.5 36 7830.0 37 8936.5 38 9340.0 39 9334.5 40 9232.0 41 9394.5 42 9664.5 43 10337.0 44 11316.0 45 12150.5 46 12677.0 47 13313.0 48 14364.0 49 15078.5 50 15344.0 51 15081.0 52 14860.5 53 15410.0 54 15849.0 55 15388.5 56 14169.5 57 12946.0 58 12545.5 59 11994.5 60 10691.0 61 9332.0 62 7972.0 63 6029.5 64 4510.0 65 3746.5 66 2780.0 67 2134.0 68 1510.5 69 896.5 70 687.5 71 573.5 72 529.5 73 568.5 74 445.0 75 323.0 76 241.0 77 124.0 78 73.5 79 34.0 80 13.5 81 7.0 82 2.0 83 1.5 84 2.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018892499455451486 2 0.002889441093186698 3 2.2226469947589985E-4 4 0.1393599665713892 5 0.04200802820094507 6 0.01266908787012629 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.2226469947589985E-4 30-31 1.1113234973794992E-4 32-33 2.2226469947589985E-4 34-35 3.333970492138497E-4 36-37 4.445293989517997E-4 38-39 0.002556044043972848 40-41 0.0 42-43 0.0013335881968553989 44-45 7.779264481656495E-4 46-47 1.1113234973794992E-4 48-49 7.779264481656495E-4 50-51 0.0020003822952830985 52-53 2.2226469947589985E-4 54-55 0.0 56-57 7.779264481656495E-4 58-59 0.001222455847117449 60-61 0.0010001911476415493 62-63 0.0 64-65 8.890587979035994E-4 66-67 5.556617486897496E-4 68-69 7.779264481656495E-4 70-71 7.779264481656495E-4 72-73 0.0 74-75 0.0 76-77 0.0030005734429246478 78-79 8.890587979035994E-4 80-81 0.0 82-83 0.0 84-85 2.2226469947589985E-4 86-87 2.2226469947589985E-4 88-89 0.0 90-91 3.333970492138497E-4 92-93 8.890587979035994E-4 94-95 6.667940984276994E-4 96-97 3.333970492138497E-4 98-99 0.0 100-101 1.1113234973794992E-4 102-103 0.0 104-105 0.0 106-107 2.2226469947589985E-4 108-109 3.333970492138497E-4 110-111 0.0 112-113 4.445293989517997E-4 114-115 0.0013335881968553989 116-117 7.779264481656495E-4 118-119 2.2226469947589985E-4 120-121 0.0 122-123 4.445293989517997E-4 124-125 3.333970492138497E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 449914.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.19668847257632 #Duplication Level Percentage of deduplicated Percentage of total 1 73.22954876401865 36.026512975307625 2 13.939014528769999 13.71506710773226 3 5.20342036012501 7.67973151346793 4 2.5525570478509048 5.023094155664002 5 1.38524337979687 3.407469350728267 6 0.8493612527747468 2.5071456572061823 7 0.5676964378413556 1.9550149359630724 8 0.40140573826693404 1.579826644529829 9 0.28246703232701387 1.2506798333848723 >10 1.4504210089168912 12.735261627196378 >50 0.08141889536244155 2.732668421088736 >100 0.05292228198468627 4.891431967684167 >500 0.00135698158935093 0.4590793935368016 >1k 0.0022616359822515497 2.419346178638927 >5k 9.046543929006199E-4 3.617670237870957 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9444 2.099067821850398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6813 1.5142893975293055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4605 1.0235289410865187 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1751 0.38918548878230064 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1675 0.3722933716221322 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1649 0.3665144894357588 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1192 0.26493952177527264 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 816 0.18136799477233426 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 694 0.15425170143627448 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 553 0.12291237881017261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 463 0.10290855585734161 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 462 0.10268629115786573 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.10201949705943804 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1113234973794992E-4 0.0 22-23 0.0 0.0 0.0 6.667940984276995E-4 0.0 24-25 0.0 0.0 0.0 0.0015558528963312988 0.0 26-27 0.0 0.0 0.0 0.0030005734429246478 0.0 28-29 0.0 0.0 0.0 0.005334352787421596 0.0 30-31 0.0 0.0 0.0 0.01289135256960219 0.0 32-33 0.0 0.0 0.0 0.02622723453815618 0.0 34-35 0.0 0.0 0.0 0.040785572353827626 0.0 36-37 0.0 0.0 0.0 0.0674573362909356 0.0 38-39 0.0 0.0 0.0 0.11613330547615766 0.0 40-41 0.0 0.0 0.0 0.18070120067390655 0.0 42-43 0.0 0.0 0.0 0.23115528745493583 0.0 44-45 0.0 0.0 0.0 0.29038882986526315 0.0 46-47 0.0 0.0 0.0 0.3655142982881173 0.0 48-49 0.0 0.0 0.0 0.4514196046355526 0.0 50-51 0.0 0.0 0.0 0.5491049400552106 0.0 52-53 0.0 0.0 0.0 0.6460123490267029 0.0 54-55 0.0 0.0 0.0 0.7406971110034362 0.0 56-57 0.0 0.0 0.0 0.8482732255497717 0.0 58-59 0.0 0.0 0.0 0.976408824797628 0.0 60-61 0.0 0.0 0.0 1.1401067759616281 0.0 62-63 0.0 0.0 0.0 1.2919135657036678 0.0 64-65 0.0 0.0 0.0 1.4316069293242708 0.0 66-67 0.0 0.0 0.0 1.5895259983018977 0.0 68-69 0.0 0.0 0.0 1.7777841987579848 0.0 70-71 0.0 0.0 0.0 2.005938912769996 0.0 72-73 0.0 0.0 0.0 2.2335379650333174 0.0 74-75 0.0 0.0 0.0 2.4445782971856844 0.0 76-77 0.0 0.0 0.0 2.6721773494490058 0.0 78-79 0.0 0.0 0.0 2.9506750178923085 0.0 80-81 0.0 0.0 0.0 3.2495099063376554 0.0 82-83 0.0 0.0 0.0 3.563792191396578 0.0 84-85 0.0 0.0 0.0 3.872073329569651 0.0 86-87 0.0 0.0 0.0 4.18657787932805 0.0 88-89 0.0 0.0 0.0 4.542090266139752 0.0 90-91 0.0 0.0 0.0 4.947834475033006 0.0 92-93 0.0 0.0 0.0 5.405477491253884 0.0 94-95 0.0 0.0 0.0 5.846117257964856 0.0 96-97 0.0 0.0 0.0 6.259085069591077 0.0 98-99 0.0 0.0 0.0 6.713838644718768 0.0 100-101 0.0 0.0 0.0 7.257386967287082 0.0 102-103 0.0 0.0 0.0 7.829829700787261 0.0 104-105 0.0 0.0 0.0 8.407606787074863 0.0 106-107 0.0 0.0 0.0 8.95748965357824 0.0 108-109 0.0 0.0 0.0 9.535489004565317 0.0 110-111 0.0 0.0 0.0 10.188391559275773 0.0 112-113 0.0 0.0 0.0 10.888969892023809 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 75 0.0 87.37056 2 GGTATCA 2635 0.0 74.86423 1 GTATCAA 5420 0.0 61.68657 1 TATCACG 50 7.3923093E-6 59.57083 2 ATAACGC 110 0.0 59.57083 3 TAACGCA 130 0.0 50.406086 4 TATCAAC 7135 0.0 46.838455 2 ATCAACG 7170 0.0 46.52673 3 TCAACGC 7240 0.0 45.994606 4 CAACGCA 7395 0.0 44.905025 5 AACGCAG 7545 0.0 44.007385 6 GTATAAC 220 0.0 40.634563 1 GATATAG 155 1.1823431E-10 38.44991 1 ACGCAGA 8625 0.0 38.419365 7 GTCCAAG 330 0.0 37.925594 1 CGCAGAG 8730 0.0 37.820988 8 CTTACTA 130 4.1984094E-8 36.610085 9 GTGGTAT 975 0.0 36.064045 1 GCAGAGT 9210 0.0 35.785263 9 CCAAGAC 295 0.0 34.328953 3 >>END_MODULE