FastQCFastQC Report
Fri 27 May 2016
SRR939994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594337
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG38930.15005760624005285No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC34390.13255795218585711No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG31820.12265176035341592No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG31530.12153394104158403No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC26440.10191428484425885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33300.042.641811
GGTATCA20450.040.553131
ATCAACG33450.040.451043
CAACGCA34750.038.9219675
TCAACGC35250.038.388424
AACGCAG37100.036.456566
GTACATG93800.032.6936341
TATCAAC42450.032.1842882
TACATGG93200.032.006652
ACATGGG92100.031.130453
ACGCAGA46050.029.7592497
TATACCG1903.67163E-828.2236165
CATGGGG66050.027.707614
CGCAGAG50250.027.1533348
ATGGGGA41700.025.5764775
ATACCGG1802.1550079E-523.171246
GTATAGG8150.021.9617311
GAGTACA69900.021.4239461
AGTACAT66850.020.883372
GCAGAGT66700.019.9117729
Produced by FastQC (version 0.11.5)