FastQCFastQC Report
Fri 27 May 2016
SRR939994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594337
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG37750.14550923800570242No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC37250.1435819633301302No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG32760.1262750367434917No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG30670.11821902859959982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.057.009361
GTATCAA50150.055.9860231
ATCAACG57600.047.389313
CAACGCA59100.046.1286435
TCAACGC59450.045.9146234
AACGCAG62000.044.0517356
TATCAAC64800.042.4925462
ACGCAGA76100.035.9616667
CGCAGAG78500.034.786428
TACATGG100300.034.3457262
ACATGGG98050.034.3430023
GTACATG104350.034.1044921
CATGGGG71300.028.336594
GCAGAGT97050.027.9534849
ATGGGGA45850.023.627885
AGAGTAC88900.023.2217210-11
CATGGGA40750.022.0844574
AGTACTT49550.021.8521612-13
CAGAGTA93100.021.72680910-11
GTACGAT1400.003158839221.247547
Produced by FastQC (version 0.11.5)