FastQCFastQC Report
Fri 27 May 2016
SRR939993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155883
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107160.9270834504876359No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59320.513200730523764No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40440.3498623995681224No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37430.3238217016774189No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33870.2930227367302746No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24740.21403550359335677No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23020.19915510479866907No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22560.1951754632605549No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20910.1809006620912324No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA18120.15676327102310528No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16930.14646811139189694No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16590.1435266372115517No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16150.139720023566399No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15990.13833580042270716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG150.0019914272024.7649110-111
CCGCTCC3100.01845.7229108-109
CCGCTCG900.01658.4757108-109
CGCGTTC200.00353992851518.5737110-111
CGTCGCC1400.01421.5505108-109
CGACGCC401.1252454E-51243.8568108-109
CCGTACC401.1252454E-51243.8568108-109
CCGCGCC2200.01243.8568108-109
CGGCGGT250.00553053521214.8589110-111
CGTTGCG250.00553053521214.8589110-111
CTCCGCC1900.01118.949110-111
CCGCACC1200.01036.5472108-109
CGTCGGC300.0079631021012.38245110-111
CAGCGCG502.1970878E-5995.08545108-109
CGGTGCG350.004143405867.75635110-111
GGTCGCC1203.45608E-10829.23785108-109
CGTTGTC754.066785E-5809.90594110-111
TGTCGCT754.066785E-5809.90594110-111
GGACGCC654.824836E-5765.4504108-109
CCGCGCG706.025203E-5710.77527108-109
Produced by FastQC (version 0.11.5)