##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1155883 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.823288343197365 34.0 31.0 34.0 30.0 34.0 2 32.009803760415195 34.0 31.0 34.0 30.0 34.0 3 32.08508646636381 34.0 31.0 34.0 30.0 34.0 4 35.36732783508366 37.0 35.0 37.0 33.0 37.0 5 35.40433417569079 37.0 35.0 37.0 33.0 37.0 6 35.47465963250606 37.0 37.0 37.0 35.0 37.0 7 35.38861891731256 37.0 37.0 37.0 33.0 37.0 8 35.41214551991854 37.0 37.0 37.0 35.0 37.0 9 37.076235224499364 39.0 39.0 39.0 35.0 39.0 10-11 37.05079363568804 39.0 39.0 39.0 35.0 39.0 12-13 36.995255575174994 39.0 38.0 39.0 34.0 39.0 14-15 38.50010295159631 41.0 39.0 41.0 34.5 41.0 16-17 38.51382017038057 41.0 39.0 41.0 34.0 41.0 18-19 38.53064194213428 41.0 39.5 41.0 34.0 41.0 20-21 38.575988227182165 41.0 39.0 41.0 34.0 41.0 22-23 38.5594515188821 41.0 39.0 41.0 34.0 41.0 24-25 38.5702324543228 41.0 39.0 41.0 34.0 41.0 26-27 38.517831822078875 41.0 39.0 41.0 34.0 41.0 28-29 38.469297930672916 41.0 39.0 41.0 34.0 41.0 30-31 38.39210672706494 41.0 39.0 41.0 34.0 41.0 32-33 38.28159121641204 40.5 39.0 41.0 33.5 41.0 34-35 38.187048343128154 40.0 38.0 41.0 33.0 41.0 36-37 38.36353376596074 41.0 39.0 41.0 34.0 41.0 38-39 38.31999778524297 41.0 39.0 41.0 33.0 41.0 40-41 38.266068451564735 41.0 39.0 41.0 33.0 41.0 42-43 38.20271774911475 41.0 39.0 41.0 33.0 41.0 44-45 38.10945398452958 41.0 38.0 41.0 33.0 41.0 46-47 37.991923057956555 41.0 38.0 41.0 33.0 41.0 48-49 37.88997891655124 40.0 38.0 41.0 33.0 41.0 50-51 37.773450686617934 40.0 38.0 41.0 33.0 41.0 52-53 37.6942415452083 40.0 38.0 41.0 32.5 41.0 54-55 37.547272950636014 40.0 38.0 41.0 32.0 41.0 56-57 37.32735147069383 40.0 37.5 41.0 31.0 41.0 58-59 37.15856319367964 40.0 37.0 41.0 31.0 41.0 60-61 36.90693954318906 40.0 36.5 41.0 31.0 41.0 62-63 36.70497619568762 40.0 36.0 41.0 31.0 41.0 64-65 36.432188205899735 39.0 36.0 41.0 30.0 41.0 66-67 36.09987948607255 39.0 35.0 41.0 30.0 41.0 68-69 35.677714786012075 39.0 35.0 40.5 29.0 41.0 70-71 35.236027781358494 37.5 35.0 40.0 29.0 41.0 72-73 34.838218487511284 37.0 34.5 39.5 28.5 41.0 74-75 34.427500447709676 37.0 34.0 39.0 28.0 41.0 76-77 33.95808788605767 36.0 34.0 39.0 28.0 40.5 78-79 33.45109972203069 36.0 34.0 38.5 26.0 39.5 80-81 32.95284687117987 35.0 34.0 37.0 26.0 39.0 82-83 32.70183098116332 35.0 34.0 37.0 26.0 39.0 84-85 32.242415971166636 35.0 34.0 36.5 25.0 38.5 86-87 31.573769144454932 35.0 34.0 36.0 11.0 37.0 88-89 16.03927819684172 18.0 16.5 18.5 2.0 19.5 90-91 2.7423740119025886 2.0 2.0 2.0 2.0 2.0 92-93 2.2892684640227428 2.0 2.0 2.0 2.0 2.0 94-95 2.0996151859660537 2.0 2.0 2.0 2.0 2.0 96-97 2.0000134096617046 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.14904961594179866 1101 2 0.03053423639397579 1101 3 -0.01552890597213974 1101 4 0.032369939618781984 1101 5 0.020695683746382088 1101 6 -0.07153907656812208 1101 7 -0.09423384304182747 1101 8 0.03353941737404398 1101 9 -0.034470126913099364 1101 10-11 0.004633584677208091 1101 12-13 0.047485193672521575 1101 14-15 3.828488641346439E-4 1101 16-17 0.04217380494674927 1101 18-19 0.07637411489186263 1101 20-21 0.05028424413920618 1101 22-23 -0.05189679481165399 1101 24-25 0.07809624864223252 1101 26-27 0.13365801661842625 1101 28-29 0.049259326121358527 1101 30-31 0.001954258623115379 1101 32-33 0.034297293821907715 1101 34-35 0.05681014694035014 1101 36-37 0.09262697983536583 1101 38-39 0.052324162260234175 1101 40-41 -0.103561507781194 1101 42-43 -0.027641030400566535 1101 44-45 -0.07046375555297857 1101 46-47 -0.0627617983167923 1101 48-49 -0.07481013257896763 1101 50-51 -0.12081487409159308 1101 52-53 -0.09564792064209371 1101 54-55 -0.0937468506433845 1101 56-57 -0.1343791370440215 1101 58-59 -0.11999773998257979 1101 60-61 -0.10789621151265294 1101 62-63 -0.11191875297659948 1101 64-65 -0.057225084203722076 1101 66-67 -0.12141921425138236 1101 68-69 -0.20989018012190286 1101 70-71 -0.22706041237245955 1101 72-73 -0.23942180959061687 1101 74-75 -0.15766394158869446 1101 76-77 -0.201658301226729 1101 78-79 -0.05795327378575621 1101 80-81 0.020166724541432757 1101 82-83 -0.027141095453146136 1101 84-85 -0.002776097728165894 1101 86-87 0.05219675987674677 1101 88-89 0.7741638821821688 1101 90-91 -0.12829122223658063 1101 92-93 -0.23114295061306223 1101 94-95 -0.060332865329295604 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.07495426467225386 1102 2 0.1548816654871672 1102 3 0.15764806144211718 1102 4 0.1767162273549019 1102 5 0.11248590231626565 1102 6 0.18114025916206344 1102 7 0.04581181124111566 1102 8 0.06749364895539145 1102 9 0.09096045004290687 1102 10-11 0.08871051214396886 1102 12-13 0.019940252737747244 1102 14-15 0.03022134683822486 1102 16-17 0.12601275946367707 1102 18-19 0.038629436469214795 1102 20-21 -0.0030234086599776333 1102 22-23 2.0273152515670745E-4 1102 24-25 0.051539722933164 1102 26-27 0.10242535097899008 1102 28-29 0.11165046919046517 1102 30-31 0.08923440471681943 1102 32-33 0.08774076811283749 1102 34-35 0.10559346029992867 1102 36-37 0.19573660718227615 1102 38-39 0.16235440822267577 1102 40-41 0.07161226383324504 1102 42-43 0.12222885489305213 1102 44-45 0.1840799869568599 1102 46-47 0.19003566787050374 1102 48-49 0.2101262367641752 1102 50-51 0.15156713812092448 1102 52-53 0.1678737366083709 1102 54-55 0.1586808158520796 1102 56-57 0.1600031034399123 1102 58-59 0.1563961423435103 1102 60-61 0.13603405157014947 1102 62-63 0.055845899194835624 1102 64-65 0.0645799720081115 1102 66-67 0.08843126797198408 1102 68-69 0.06807820428716127 1102 70-71 0.1671934254407006 1102 72-73 0.22160027566875584 1102 74-75 0.10962419400452461 1102 76-77 0.1617880264568896 1102 78-79 0.1187641832706845 1102 80-81 0.11348065480451908 1102 82-83 0.04052083458166322 1102 84-85 -0.0865682566050694 1102 86-87 -0.03908840818807846 1102 88-89 1.0791430524155743 1102 90-91 0.19175614408197195 1102 92-93 0.022532219163802214 1102 94-95 0.13779188499884487 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.027624543544138902 1103 2 0.07490200309140249 1103 3 0.010440414502923545 1103 4 -0.00510602198412613 1103 5 -0.04203107958326768 1103 6 -0.07070029402076727 1103 7 -0.03545318774200723 1103 8 0.0017177693540091354 1103 9 0.05726510735427581 1103 10-11 -0.029678749600996923 1103 12-13 -2.082117731347921E-4 1103 14-15 -0.0010863611137708062 1103 16-17 0.013100414537909444 1103 18-19 0.04637806368092612 1103 20-21 0.08761925963370487 1103 22-23 0.09634672176311199 1103 24-25 0.01048826879446807 1103 26-27 0.004684858808957415 1103 28-29 0.09169521191977026 1103 30-31 0.0985103860062253 1103 32-33 0.09044363571503311 1103 34-35 0.08341123536602879 1103 36-37 0.10362556386318289 1103 38-39 0.060312512757960235 1103 40-41 0.08787624593615817 1103 42-43 0.059399894144625875 1103 44-45 0.06271940123386344 1103 46-47 0.08655183626586904 1103 48-49 0.03476321052867348 1103 50-51 -0.02680381978023405 1103 52-53 -0.07343397134362561 1103 54-55 -0.07121749612677775 1103 56-57 -0.05767840967784821 1103 58-59 -0.046827367926979946 1103 60-61 -0.10706336555411156 1103 62-63 -0.1928748654990855 1103 64-65 -0.10082779695670752 1103 66-67 -0.002496126780918928 1103 68-69 -0.08778105949156867 1103 70-71 1.627156583126066E-4 1103 72-73 0.05265376356787499 1103 74-75 -0.08328001587955214 1103 76-77 -0.11804317142799903 1103 78-79 -0.03671273354851223 1103 80-81 -0.09051771818714371 1103 82-83 -0.17415478063086454 1103 84-85 -0.10753429280600812 1103 86-87 -0.05716650458832362 1103 88-89 0.5952402661637954 1103 90-91 -0.5942942931765192 1103 92-93 -0.30019505356347054 1103 94-95 -0.10584447863751878 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.09208257074781656 1104 2 0.1144752056795113 1104 3 0.1684519082938678 1104 4 0.24324498801539818 1104 5 0.09485207905370174 1104 6 0.25110941176594537 1104 7 0.1489618747504622 1104 8 0.257647885935981 1104 9 0.26268246056731215 1104 10-11 0.29161198193166626 1104 12-13 0.34301954869129503 1104 14-15 0.30846123070685394 1104 16-17 0.2681611898755776 1104 18-19 0.25493319259333447 1104 20-21 0.23977644617580296 1104 22-23 0.10825390191165951 1104 24-25 0.18225102759656409 1104 26-27 0.16897044261389738 1104 28-29 0.12306219120099371 1104 30-31 0.18207236515956993 1104 32-33 0.21310277137902744 1104 34-35 0.1288179204705031 1104 36-37 0.06192604648548894 1104 38-39 0.11980859076668082 1104 40-41 0.1918826321874505 1104 42-43 0.08785396830260339 1104 44-45 0.01604514754477293 1104 46-47 -0.011123831312943366 1104 48-49 -0.008414862854763783 1104 50-51 -0.0867289968817957 1104 52-53 -0.06611827935969217 1104 54-55 -0.06436944725806626 1104 56-57 0.01120615188847296 1104 58-59 0.004772117665595488 1104 60-61 -0.175657111558138 1104 62-63 -0.12539706941340256 1104 64-65 -0.21494824428657466 1104 66-67 0.0201188019948475 1104 68-69 -0.01942497118008646 1104 70-71 -0.1949041974952408 1104 72-73 -0.27635472342396383 1104 74-75 -0.24941772216314462 1104 76-77 -0.27262315118782254 1104 78-79 -0.2292742852468308 1104 80-81 -0.05668446949234607 1104 82-83 -0.14739061524595343 1104 84-85 -0.1081106264109053 1104 86-87 -0.037694831704680354 1104 88-89 0.5572776922268616 1104 90-91 -0.5815844474622125 1104 92-93 -0.30019505356347054 1104 94-95 -0.10584447863751878 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.37935908155598597 1105 2 -0.28856127455151537 1105 3 -0.30004160114116374 1105 4 -0.22392421966852538 1105 5 -0.21312982946967907 1105 6 -0.27194377264537195 1105 7 -0.2244912190619175 1105 8 -0.3845330005180614 1105 9 -0.323797770459997 1105 10-11 -0.2816054130655772 1105 12-13 -0.27049172663040366 1105 14-15 -0.3191951586421098 1105 16-17 -0.2986240340029056 1105 18-19 -0.21354120612695482 1105 20-21 -0.23863269299265966 1105 22-23 -0.4319571653155876 1105 24-25 -0.3126124369636756 1105 26-27 -0.40583491121744686 1105 28-29 -0.35290573111041823 1105 30-31 -0.37699920800615416 1105 32-33 -0.41019389769417103 1105 34-35 -0.256109741935191 1105 36-37 -0.43311896986478615 1105 38-39 -0.3778691034901982 1105 40-41 -0.421568479773029 1105 42-43 -0.3384406056700229 1105 44-45 -0.4960607824392298 1105 46-47 -0.4723262491722693 1105 48-49 -0.5307860778715394 1105 50-51 -0.5870584797600102 1105 52-53 -0.5639584146918608 1105 54-55 -0.6261225368236154 1105 56-57 -0.5970482612710271 1105 58-59 -0.5847654738181092 1105 60-61 -0.5886414266159647 1105 62-63 -0.5743958803022622 1105 64-65 -0.6642057576286788 1105 66-67 -0.6110497120409164 1105 68-69 -0.4837679164951254 1105 70-71 -0.6981590353372908 1105 72-73 -0.5471811556658679 1105 74-75 -0.5486263970120291 1105 76-77 -0.5451741484840085 1105 78-79 -0.4643155502345806 1105 80-81 -0.5499171530613722 1105 82-83 -0.4707708052206172 1105 84-85 -0.6008998706175319 1105 86-87 -0.6140959302168802 1105 88-89 0.4019670369416879 1105 90-91 -0.7321832498574219 1105 92-93 -0.30019505356347054 1105 94-95 -0.10584447863751878 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.17824108944529016 1106 2 -0.16372631634847679 1106 3 -0.1104036370994983 1106 4 -0.15545214221980785 1106 5 -0.09178272051143921 1106 6 -0.09199745806787263 1106 7 -0.27818958601676513 1106 8 -0.15217799544247157 1106 9 -0.19359461065048578 1106 10-11 -0.20367093925765545 1106 12-13 -0.2200134630949364 1106 14-15 -0.17032183716317206 1106 16-17 -0.2100688054651485 1106 18-19 -0.21261499633141767 1106 20-21 -0.2138897085464464 1106 22-23 -0.12774067953063906 1106 24-25 -0.24552166908500084 1106 26-27 -0.4307569038459178 1106 28-29 -0.19764653543794708 1106 30-31 -0.06938667641146878 1106 32-33 -0.023134264635402246 1106 34-35 -0.08506325090432654 1106 36-37 0.0034625699802859344 1106 38-39 -0.02818964906489896 1106 40-41 -0.0331462725391205 1106 42-43 -0.3652211494733777 1106 44-45 -0.06080604919718269 1106 46-47 -0.14086032374594737 1106 48-49 0.07390407276103161 1106 50-51 -0.0011313161625423618 1106 52-53 0.031629740450838995 1106 54-55 0.10221151186115662 1106 56-57 -0.14105531940525395 1106 58-59 0.047807372803731596 1106 60-61 0.09980752113612823 1106 62-63 0.1350983662282843 1106 64-65 0.13593356558072145 1106 66-67 0.06409540210874098 1106 68-69 0.16509331213799783 1106 70-71 0.12440502268111686 1106 72-73 0.28436073327070943 1106 74-75 0.03870200380279698 1106 76-77 -0.012265767971541663 1106 78-79 0.08204773929199405 1106 80-81 0.22212793451807755 1106 82-83 0.2552461860793471 1106 84-85 0.21305885019782522 1106 86-87 0.06877378725336669 1106 88-89 0.9476217878971909 1106 90-91 -0.27195270230698965 1106 92-93 -0.26532473656058864 1106 94-95 -0.10584447863751878 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.12047709864579303 1107 2 0.13136480675904494 1107 3 0.1368115270844541 1107 4 0.2470790101120457 1107 5 0.2187940542582183 1107 6 0.2351137973609525 1107 7 0.22743366356719008 1107 8 0.13678088753840711 1107 9 0.19566382178852848 1107 10-11 0.24471563378288863 1107 12-13 0.2775544226054407 1107 14-15 0.20987136817839058 1107 16-17 0.2345616284350811 1107 18-19 0.23780997424130135 1107 20-21 0.16379263914198816 1107 22-23 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2213 90-91 -0.7321832498574219 2213 92-93 -0.30019505356347054 2213 94-95 -0.10584447863751878 2213 96-97 0.0 2213 98-99 0.0 2213 100-101 0.0 2213 102-103 0.0 2213 104-105 0.0 2213 106-107 0.0 2213 108-109 0.0 2213 110-111 0.0 2213 112-113 0.0 2213 114-115 0.0 2213 116-117 0.0 2213 118-119 0.0 2213 120-121 0.0 2213 122-123 0.0 2213 124-125 0.0 2214 1 0.08684565660503196 2214 2 -0.01802306730375136 2214 3 -0.07936678437769729 2214 4 -0.08980840855411287 2214 5 -0.05246291331025077 2214 6 0.03261778663545556 2214 7 0.026327887680629658 2214 8 0.01647952426304755 2214 9 0.039422410569017075 2214 10-11 -0.010907603832137625 2214 12-13 -0.04622617094032222 2214 14-15 -0.13263557206628462 2214 16-17 -0.7446346875883236 2214 18-19 -0.18615540104195105 2214 20-21 -0.1481310290960991 2214 22-23 -0.10270560690309338 2214 24-25 -0.15037518402571237 2214 26-27 -0.15573875254983705 2214 28-29 -0.23025863739530195 2214 30-31 -0.10262673121570742 2214 32-33 -0.054063774990211755 2214 34-35 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0.0 2214 102-103 0.0 2214 104-105 0.0 2214 106-107 0.0 2214 108-109 0.0 2214 110-111 0.0 2214 112-113 0.0 2214 114-115 0.0 2214 116-117 0.0 2214 118-119 0.0 2214 120-121 0.0 2214 122-123 0.0 2214 124-125 0.0 2215 1 0.16744308732501167 2215 2 -0.01707064449792739 2215 3 -0.009753826287642653 2215 4 -0.08894696191484996 2215 5 -0.047792289437339264 2215 6 -0.028348066719701137 2215 7 -0.11892738398988456 2215 8 -0.19200958005603042 2215 9 -0.23170224245585302 2215 10-11 -0.09841318383218578 2215 12-13 -0.16075025893637473 2215 14-15 -0.19961471873553904 2215 16-17 -0.18641148296057253 2215 18-19 -0.09281616710753582 2215 20-21 -0.10813518213240769 2215 22-23 -0.07140183876793316 2215 24-25 -0.2150524280852153 2215 26-27 -0.14969089343234288 2215 28-29 -0.1571920544061598 2215 30-31 -0.2427129375583874 2215 32-33 -0.182100265170817 2215 34-35 -0.13918050890026734 2215 36-37 -0.06506319410932804 2215 38-39 -0.036781210257203156 2215 40-41 -0.0044781484866689425 2215 42-43 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114-115 0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.11251887495709667 2216 2 -0.030190351879138433 2216 3 -0.023434985875198322 2216 4 -7.556844054903422E-4 2216 5 0.0010006724767279707 2216 6 -0.015157666855124319 2216 7 0.018813072645087914 2216 8 0.02760864206167213 2216 9 -0.03201352983329997 2216 10-11 -0.049341058744005295 2216 12-13 -0.04110407241113023 2216 14-15 -0.016516017120821402 2216 16-17 -0.030972778393113742 2216 18-19 -0.03275136085543551 2216 20-21 0.004815777001091703 2216 22-23 -0.025903050398625282 2216 24-25 -0.10650099401365054 2216 26-27 -0.18595301154265087 2216 28-29 -0.083171614179534 2216 30-31 -0.13718408805210913 2216 32-33 -0.10714086525862854 2216 34-35 -0.10441387344408781 2216 36-37 -0.14721771825994523 2216 38-39 -0.2334207685205527 2216 40-41 -0.22039571528024737 2216 42-43 -0.22130747647484128 2216 44-45 -0.24373133047691908 2216 46-47 -0.29567409187604454 2216 48-49 -0.2827796859674905 2216 50-51 -0.3418280920119656 2216 52-53 -0.25959318820608246 2216 54-55 -0.2924682351614507 2216 56-57 -0.3738356715314879 2216 58-59 -0.23455841777852982 2216 60-61 -0.24964079955483953 2216 62-63 -0.34984420611773004 2216 64-65 -0.4468392541352202 2216 66-67 -0.536690618853676 2216 68-69 -0.4792646335784241 2216 70-71 -0.38785160374759897 2216 72-73 -0.4596992637721229 2216 74-75 -0.48910765497902275 2216 76-77 -0.5029296243982202 2216 78-79 -0.4937548111263368 2216 80-81 -0.5254137099465765 2216 82-83 -0.39950439349777866 2216 84-85 -0.432597180891495 2216 86-87 -0.5013307737605537 2216 88-89 -1.1586667982693974 2216 90-91 -0.7321832498574219 2216 92-93 -0.30019505356347054 2216 94-95 -0.10584447863751878 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13123.0 3 2236.0 4 1407.0 5 1219.0 6 1246.0 7 1384.0 8 1620.0 9 1841.0 10 2200.0 11 2752.0 12 3283.0 13 3829.0 14 4549.0 15 5193.0 16 5825.0 17 6786.0 18 8628.0 19 10536.0 20 11611.0 21 16175.0 22 19345.0 23 24316.0 24 37426.0 25 64183.0 26 116188.0 27 304586.0 28 466918.0 29 16819.0 30 659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.519859708984384 20.20342889375482 14.73972711771001 23.536984279550786 2 12.925035881969732 21.046256811950794 43.91503218779226 22.113675118287215 3 20.186652841812453 25.34751823383448 29.19568602232806 25.270142902025 4 11.338437649844812 17.522733134723143 40.76038505680471 30.37844415862734 5 12.158743210784845 38.887672300002166 35.22238331205505 13.731201177157942 6 28.64277785900476 37.76264552727223 19.841627569572353 13.752949044150661 7 27.225938957489642 32.21666898812423 22.779122108379482 17.77826994600665 8 23.153381440855174 37.91854365883052 21.03456837759531 17.893506522718994 9 24.384993982955024 19.55933256220569 22.941249244084393 33.1144242107549 10-11 23.7140217204169 26.69052169316472 29.965368107686757 19.630088478731622 12-13 26.44854076375067 24.396478297789137 28.875475132617535 20.279505805842653 14-15 20.750283549459592 29.0413043534683 26.882738131800537 23.32567396527157 16-17 19.921825659801467 30.490025763964706 29.728786255988954 19.85936232024487 18-19 20.65556565455119 29.578021086183753 30.035633928760696 19.730779330504365 20-21 21.865449444156297 28.702939078457472 28.118826271969116 21.31278520541711 22-23 20.416375942627187 30.290121323305176 28.538726172344237 20.754776561723396 24-25 21.77664175353388 28.772202722939948 29.56752543293742 19.883630090588753 26-27 20.241754572045785 29.5541157712329 29.941395452653946 20.262734204067367 28-29 19.556498184482844 31.000917924090015 27.97289312197144 21.469690769455706 30-31 20.672896824332568 28.796167086115116 28.930782786839153 21.600153302713164 32-33 20.086964684223116 29.732630314009818 29.83861095286111 20.341794048905957 34-35 20.898379288880392 29.625701250517462 29.50747978923445 19.968439671367697 36-37 20.13094762268121 28.987431242981547 29.983683455577648 20.897937678759597 38-39 20.800937465124065 30.18925790932127 28.945922727473285 20.06388189808138 40-41 21.96766454736336 28.70454881679201 29.569991080412116 19.757795555432516 42-43 19.709131460537098 30.48959107452917 29.13681575038304 20.664461714550693 44-45 20.037469222636915 29.76294293016069 28.70042097722778 21.499166869974616 46-47 21.774392390925378 28.999864173104022 28.734266357408146 20.49147707856245 48-49 20.9079210040813 29.250248186991325 28.94221514730087 20.899615661626505 50-51 19.144601265527104 30.49411618140952 29.81364014665857 20.547642406404805 52-53 19.776612338792074 30.136960228673665 29.27683857276212 20.80958885977214 54-55 19.746274622444318 28.746271161805627 29.815738292659294 21.691715923090765 56-57 20.877459050786282 28.88955889134108 28.112793422863735 22.120188635008905 58-59 21.75596492032498 29.39133978092939 27.562910779032133 21.2897845197135 60-61 20.429143780123074 28.363251297925483 30.74143317273461 20.466171749216834 62-63 20.45185369107427 28.54934279680556 30.071815226973662 20.926988285146507 64-65 20.662359998684988 30.07426363590747 28.287965380549224 20.975410984858318 66-67 21.00753276932008 30.17602127550972 27.4033358047484 21.4131101504218 68-69 21.226962337753346 28.56700398571131 29.415059880835276 20.79097379570007 70-71 22.499076463621318 28.393920491952905 28.58546236946127 20.52154067496451 72-73 21.3549295214135 29.045630050792337 29.06544174453643 20.533998683257735 74-75 20.21882837622839 30.650290730117145 29.2621312018604 19.868749691794065 76-77 20.782293709657466 28.593594680430456 29.603298949807204 21.020812660104873 78-79 20.731390801813337 28.65284458594318 29.346039381250648 21.269725230992837 80-81 21.527957414374985 29.15753584056518 28.979273853841608 20.33523289121823 82-83 20.849971169015323 28.81043746531306 30.320592763314703 20.018998602356916 84-85 20.4260990421596 29.211125880983474 30.087513296129405 20.275261780727522 86-87 19.724649203933655 30.752047590085745 28.277333716305712 21.245969489674884 88-89 29.41407748164305 18.970864571035552 34.975799038817954 16.63925890850344 90-91 30.425652597514958 14.88613894198931 16.64319429201902 38.04501416847671 92-93 22.4127297397311 17.32758182834007 35.37356968197418 24.88611874995465 94-95 15.082406962531477 6.772692628524657 27.155249991001046 50.98965041794282 96-97 25.896787793649434 7.047670357372432 14.725563550126633 52.3299782988515 98-99 57.09309040779448 14.11849450747344 18.785744890925688 10.002670193806393 100-101 30.745099935612075 6.855120004226857 12.595227412957644 49.80455264720342 102-103 22.751040768624343 1.9700609016767554 37.056342118972644 38.22255621072625 104-105 40.93667943695923 4.536696655331238 41.20821316344539 13.318410744264131 106-107 4.996051158130489 1.5965108740496257 6.026286320504508 87.38115164731538 108-109 17.57169056805774 10.604460218741389 21.186360598251316 50.637488614949554 110-111 39.87794847265266 2.6376131325683407 24.818444982069824 32.66599341270917 112-113 26.84468301892582 2.732335616296185 38.57361900902747 31.849362355750532 114-115 27.531962219459142 3.9158434843658245 23.414683756926376 45.13751053924865 116-117 18.871991640564907 4.033478970635036 19.78073232671373 57.31379706208632 118-119 19.44321193073273 4.154649657977445 15.01725519196401 61.384883219325815 120-121 24.721080964258228 4.105000143346411 29.55516300692253 41.61875588547284 122-123 33.67606768734891 1.5723878592532905 33.67750022383383 31.074044229563974 124-125 38.11869605906208 1.8793109424283387 19.498284288239574 40.50370871027001 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 13.0 2 42.0 3 72.5 4 93.5 5 100.0 6 89.0 7 82.0 8 73.0 9 72.5 10 80.0 11 70.5 12 83.5 13 115.0 14 130.0 15 164.5 16 236.0 17 334.0 18 471.5 19 707.0 20 1088.0 21 1599.5 22 2238.5 23 3188.5 24 4522.5 25 6089.5 26 7872.5 27 10246.5 28 13276.0 29 16938.5 30 21442.0 31 27167.0 32 33685.0 33 40597.0 34 47428.5 35 52616.0 36 55056.0 37 54906.5 38 53416.5 39 51792.0 40 49922.0 41 47702.5 42 46375.0 43 47333.0 44 49032.5 45 47713.0 46 43760.5 47 40382.5 48 38322.5 49 36860.5 50 34703.0 51 30954.5 52 25982.0 53 21200.0 54 17464.5 55 14403.5 56 12036.5 57 9870.5 58 7927.0 59 6309.5 60 4868.0 61 3716.0 62 2857.5 63 2122.5 64 1521.5 65 1131.5 66 829.5 67 636.0 68 462.5 69 337.0 70 249.0 71 173.5 72 139.0 73 102.0 74 76.5 75 58.5 76 39.5 77 25.0 78 16.0 79 9.5 80 5.5 81 3.5 82 3.5 83 3.5 84 3.5 85 5.0 86 5.0 87 2.5 88 1.0 89 2.5 90 2.5 91 2.5 92 3.5 93 2.5 94 2.0 95 2.0 96 2.5 97 3.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 8.651394648074242E-4 3 0.0411806385248334 4 0.0450737661164668 5 0.048274782136254274 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0010381673577689091 12-13 0.0018600498493359624 14-15 0.0 16-17 4.325697324037121E-4 18-19 0.008867679514276099 20-21 0.002379133528220417 22-23 3.893127591633409E-4 24-25 0.0 26-27 0.0 28-29 0.001211195250730394 30-31 0.0 32-33 9.949103845285378E-4 34-35 1.2977091972111364E-4 36-37 8.651394648074243E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 8.651394648074243E-5 46-47 0.0 48-49 4.3256973240371215E-5 50-51 8.651394648074243E-5 52-53 0.0 54-55 0.0023358765549800456 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.651394648074243E-5 66-67 0.0 68-69 3.460557859229697E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.595418394422273E-4 80-81 0.0 82-83 7.353685450863106E-4 84-85 1.2977091972111364E-4 86-87 0.0010381673577689091 88-89 19.24494088069467 90-91 26.253738483912297 92-93 40.3836720498528 94-95 44.72078921482537 96-97 48.453173893897564 98-99 93.03402680029033 100-101 51.69627894864791 102-103 53.08629852675401 104-105 74.8774313663234 106-107 81.65147337576553 108-109 12.565069301996829 110-111 39.04759391737745 112-113 52.06854846035455 114-115 86.81471221568273 116-117 66.42700861592394 118-119 71.92276380913985 120-121 80.38525525507339 122-123 75.8431432939147 124-125 50.07972260168201 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1155883.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.95919089186312 #Duplication Level Percentage of deduplicated Percentage of total 1 88.02174815594977 65.98039022657339 2 8.589315572583745 12.87696291271515 3 1.7915067056080307 4.028696793891755 4 0.5935040316005328 1.779543279993388 5 0.28981814511791026 1.0862266831909562 6 0.16417799058967897 0.7383989602112552 7 0.1093425477174165 0.5737360234866733 8 0.06918691921888986 0.41489563879589425 9 0.05553679663073092 0.3746694006148985 >10 0.2771852576875338 3.800029802235453 >50 0.021258324134115124 1.1086009206025378 >100 0.013843362428380374 2.1325306051543613 >500 0.0016150538795467247 0.9100488584100329 >1k 0.0017304148709429193 2.7556563066279476 >5k 1.1536099139619462E-4 0.5129617852611474 >10k+ 1.1536099139619462E-4 0.9266518022350734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10716 0.9270834504876359 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5932 0.513200730523764 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4044 0.3498623995681224 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3743 0.3238217016774189 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3387 0.2930227367302746 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2474 0.21403550359335677 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2302 0.19915510479866907 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2256 0.1951754632605549 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2091 0.1809006620912324 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1812 0.15676327102310528 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1693 0.14646811139189694 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1659 0.1435266372115517 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1615 0.139720023566399 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1599 0.13833580042270716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 8.651394648074243E-5 0.0 8 0.0 0.0 0.0 1.7302789296148486E-4 0.0 9 0.0 0.0 0.0 1.7302789296148486E-4 0.0 10-11 0.0 0.0 0.0 2.1628486620185608E-4 4.3256973240371215E-5 12-13 0.0 0.0 0.0 3.8931275916334097E-4 8.651394648074243E-5 14-15 0.0 0.0 0.0 4.3256973240371217E-4 8.651394648074243E-5 16-17 1.2977091972111364E-4 0.0 0.0 4.3256973240371217E-4 8.651394648074243E-5 18-19 1.7302789296148486E-4 0.0 0.0 5.190836788844546E-4 8.651394648074243E-5 20-21 1.7302789296148486E-4 0.0 0.0 5.190836788844546E-4 8.651394648074243E-5 22-23 1.7302789296148486E-4 0.0 0.0 6.488545986055682E-4 8.651394648074243E-5 24-25 1.7302789296148486E-4 0.0 0.0 9.516534112881667E-4 8.651394648074243E-5 26-27 1.7302789296148486E-4 0.0 0.0 0.0011246813042496515 8.651394648074243E-5 28-29 1.7302789296148486E-4 0.0 0.0 0.001816792876095591 8.651394648074243E-5 30-31 1.7302789296148486E-4 0.0 0.0 0.003417300885989326 8.651394648074243E-5 32-33 1.7302789296148486E-4 0.0 0.0 0.00817556794243016 8.651394648074243E-5 34-35 1.7302789296148486E-4 0.0 0.0 0.014880398794687697 8.651394648074243E-5 36-37 1.7302789296148486E-4 0.0 0.0 0.024569960800530852 8.651394648074243E-5 38-39 1.7302789296148486E-4 0.0 0.0 0.040704811819189314 8.651394648074243E-5 40-41 1.7302789296148486E-4 0.0 0.0 0.06233329843937492 8.651394648074243E-5 42-43 1.7302789296148486E-4 0.0 0.0 0.08344270138067608 8.651394648074243E-5 44-45 1.7302789296148486E-4 0.0 0.0 0.10567678562622687 8.651394648074243E-5 46-47 1.7302789296148486E-4 0.0 0.0 0.1329719357409011 8.651394648074243E-5 48-49 1.7302789296148486E-4 0.0 0.0 0.16156479505278648 8.651394648074243E-5 50-51 1.7302789296148486E-4 0.0 0.0 0.19539174812675678 8.651394648074243E-5 52-53 1.7302789296148486E-4 0.0 0.0 0.23107875105006304 8.651394648074243E-5 54-55 1.7302789296148486E-4 0.0 0.0 0.26966397118047414 8.651394648074243E-5 56-57 1.7302789296148486E-4 0.0 0.0 0.3194527473801414 8.651394648074243E-5 58-59 1.7302789296148486E-4 0.0 0.0 0.3717936850009906 8.651394648074243E-5 60-61 2.1628486620185608E-4 0.0 0.0 0.43300230213611585 8.651394648074243E-5 62-63 2.595418394422273E-4 0.0 0.0 0.4935620646726355 8.651394648074243E-5 64-65 2.595418394422273E-4 0.0 0.0 0.5528241180119442 8.651394648074243E-5 66-67 3.460557859229697E-4 0.0 0.0 0.6271395980389018 8.651394648074243E-5 68-69 3.460557859229697E-4 0.0 0.0 0.7219588833817956 8.651394648074243E-5 70-71 3.460557859229697E-4 0.0 0.0 0.8277221829545032 8.651394648074243E-5 72-73 3.460557859229697E-4 0.0 0.0 0.925353171558021 8.651394648074243E-5 74-75 3.460557859229697E-4 0.0 0.0 1.0278289411644606 8.651394648074243E-5 76-77 3.460557859229697E-4 0.0 0.0 1.1380909659541665 8.651394648074243E-5 78-79 4.3256973240371217E-4 0.0 0.0 1.1679382774900229 8.651394648074243E-5 80-81 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 82-83 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 84-85 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 86-87 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 88-89 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 90-91 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 92-93 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 94-95 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 96-97 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 98-99 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 100-101 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 102-103 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 104-105 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 106-107 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 108-109 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 110-111 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 112-113 4.3256973240371217E-4 0.0 0.0 1.1684573611689073 8.651394648074243E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGCG 15 0.001991427 2024.7649 110-111 CCGCTCC 310 0.0 1845.7229 108-109 CCGCTCG 90 0.0 1658.4757 108-109 CGCGTTC 20 0.0035399285 1518.5737 110-111 CGTCGCC 140 0.0 1421.5505 108-109 CGACGCC 40 1.1252454E-5 1243.8568 108-109 CCGTACC 40 1.1252454E-5 1243.8568 108-109 CCGCGCC 220 0.0 1243.8568 108-109 CGGCGGT 25 0.0055305352 1214.8589 110-111 CGTTGCG 25 0.0055305352 1214.8589 110-111 CTCCGCC 190 0.0 1118.949 110-111 CCGCACC 120 0.0 1036.5472 108-109 CGTCGGC 30 0.007963102 1012.38245 110-111 CAGCGCG 50 2.1970878E-5 995.08545 108-109 CGGTGCG 35 0.004143405 867.75635 110-111 GGTCGCC 120 3.45608E-10 829.23785 108-109 CGTTGTC 75 4.066785E-5 809.90594 110-111 TGTCGCT 75 4.066785E-5 809.90594 110-111 GGACGCC 65 4.824836E-5 765.4504 108-109 CCGCGCG 70 6.025203E-5 710.77527 108-109 >>END_MODULE