FastQCFastQC Report
Fri 27 May 2016
SRR939993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155883
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115090.9956890100468647No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56260.4867274629006569No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38900.33653925181008804No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37500.32442729930278413No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35810.3098064423475386No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25330.21913982643572055No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25120.21732303355962498No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24420.211267057305973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.20477851131991734No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23030.1992416187451498No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19000.1643764983134106No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18800.16264621938379575No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17920.15503299209349042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.15235105975258742No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC17170.14854444610743475No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15810.13677854938605377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16400.034.148134
TGAGTCG800.005328164429.7887945
GTACATG51400.028.9824471
TACATGG52100.027.558922
ACATGGG49600.026.3054243
GTGTAGC7200.024.0006831
CATGGGG22350.023.9909734
GAGTACT38400.023.47268312-13
AGTACTT40150.022.89413512-13
GTATAAC4750.022.580681
ACACGTC1350.002536028922.0657715
GGACCTG37550.021.548166
GTACTTT41100.021.49687414-15
ACACCGC1400.003157175821.2482536
ACCTGGA40450.021.0328838
GACCTGG39650.021.0071037
CACCGCC1703.9358306E-420.9982767
CTACAGT53500.020.8243164
TACAGTG56000.020.7457665
ATGGGCG1754.7415803E-420.42665
Produced by FastQC (version 0.11.5)