FastQCFastQC Report
Fri 27 May 2016
SRR939992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465508
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4200.01988.2769108-109
CCGCGCC3000.01855.7252108-109
CCGCTCG1300.01751.9082108-109
CCGCGCT456.7357178E-91687.0228108-109
CTCCGCC2750.01528.6674110-111
CGTCGCA200.0036239531501.3696110-111
TTTCGCT1056.91216E-111143.9006110-111
CCGCGCG1350.01124.6819108-109
CCGTTCG703.940295E-81084.5148108-109
CGTTGTT2050.01025.3256110-111
CGTCGCG250.0079741461012.2137108-109
CGCCGCT905.41786E-81000.913110-111
CAGCGCG555.9257036E-7920.1943108-109
CGTCGCC1450.0872.598108-109
CCGTGCG951.3356475E-7799.1161108-109
TGCGTCG400.0087879095750.6848110-111
GGTCGCC1601.3442332E-9632.63354108-109
CGCCGCC2950.0610.7266110-111
CGTGGCC2600.0583.9694108-109
CGGCGCC2251.0913936E-11562.34094108-109
Produced by FastQC (version 0.11.5)