FastQCFastQC Report
Fri 27 May 2016
SRR939992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465508
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.21651195353420113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.15155154390150036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.042.526281
GTATCAA50550.041.9547921
ATCAACG57750.036.5127643
TCAACGC58100.036.0877654
CAACGCA59150.035.4471475
TATCAAC61100.034.6083262
AACGCAG62000.033.8733026
CGCAGAG69850.029.8961478
ACGCAGA70100.029.8743977
CGCGATC400.005366107429.74709122-23
GTACATG53800.028.3472181
TACATGG52950.027.3359242
ACATGGG50500.026.6569523
GCAGAGT81050.025.61819
GAGTACT43350.025.25243212-13
GTCCTAC24250.025.05771
GTAGGAC25500.024.994073
GGACCGT1200.001288765724.7892446
AGAGTAC68900.023.7026910-11
AGTACTT45950.022.98197412-13
Produced by FastQC (version 0.11.5)